Planer Kryo 1060 Series User manual

Kryo 1060
(180 & 380)
OPERATOR'S MANUAL

© 2013 Planer plc
Planer plc
110 Windmill Road
Sunbury
Middlesex
U.K.
TW16 7HD
Telephone: [phone]
Facsimile: [phone]
Kryo1060 (180 & 380) Operator's Manual
MA008876
Issue 06 - Aug 2013
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of Microsoft Corporation in the United States of America and
other countries.
Planer plc reserve the right to alter products and their
specifications without notice.

Notices
This equipment contains batteries and other components which
have a limited life. To ensure the long life of the equipment and
help ensure trouble-free operation, we recommend that you join
one of our Maintenance and Support Schemes. Please contact
your supplier for details.
Good practice demands that in critical applications, reliance
should not be placed on a single source of data. Hard copies and
backup copies should be routinely kept.
All equipment supplied by Planer plc. is subject to a Warranty
Agreement and Limited Liability. In addition, software products
are not sold but are licensed for use and are subject to a Licence
Agreement. Copies of these documents will have been provided
with the equipment or as part of the software installation. Please
ensure that you read these documents before using the equipment
or software. If you cannot locate this documentation, please
contact your supplier who will be able to provide you with
copies.
The freezer is fitted with a manually-resettable thermal cutout to
prevent over heating. This will operate if the chamber is
programmed to run above +40.0ºC and will require a Service
Engineer to reset it.
Planer plc reserve the right to alter products and their
specifications without notice.
i

Symbols Used in this Manual
Failure to follow these instructions could result in
personal injury.
Failure to follow these instructions could result in
damage to equipment or samples.
This indicates first-aid advice.
Symbols Used on the Equipment
Caution: Refer to this manual and accompanying
documentation
Mains supply ON
COOL manual override
HEAT manual override
STOP key
RUN key
Alternating current
Mains supply OFF
ii

SAFETY PRECAUTIONS
IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO
OPERATING ANY EQUIPMENT
First Aid
IF IN DOUBT, SEEK IMMEDIATE
MEDICAL ATTENTION. If any quantity
of liquid nitrogen comes into contact with
the skin or eyes, immediately flood that
area of the body with large quantities of
unheated water, apply cold compresses and
seek medical attention. If the skin is
blistered or there is any possibility that eyes
have been affected, the patient should be
taken immediately to a doctor or hospital
for treatment.
Safety
Failure to comply with these instructions could
result in personal injury
POperating the system in a manner not specified within this
manual or under conditions outside of the specifications, see
Appendix A, may result in the protection offered by the
equipment being impaired.
PNever leave a freezer unattended when running a program.
PAvoid nitrogen build up: anywhere liquid nitrogen is used
must be well ventilated to reduce the risk of nitrogen build
up. Small volumes of liquid nitrogen convert to very large
volumes of gas. This can result in drowsiness, or in extreme
cases, asphyxiation. A typical run from 20 ºC to -100 ºC,
iii

taking 75 minutes, will release approximately 85 m
3
of
nitrogen gas.
POxygen-deficiency alarms must be installed. Guidance on
ventilation requirements are given in BCGA Code of Practice
CP30, The safe use of liquid nitrogen dewars up to 50 litres.
PThe exhaust port/ports must be connected to ducting that will
route the exhaust gas away from any spaces where nitrogen
build up can occur. Under no circumstances should the
exhaust duct be connected so that the exhaust is released into
a confined space.
PWhen there is a risk of reduced or insufficient ventilation
when a room is unoccupied, then alarms to indicate oxygen
deficiency must be installed and positioned outside of the
room so that operators are aware of the hazard before
entering. The operational status of such an alarm should also
be visible from outside the room.
PIf liquid nitrogen is used in an area that requires forced
ventilation, an alarm to indicate its failure should be fitted.
PWhen dispensing or potentially being exposed to liquid
nitrogen, protect the face with a shield and wear gloves, boots
and a protective apron. Prevent spillage into shoes and onto
unprotected parts of the body.
PHandle vessels containing liquid nitrogen carefully. Liquid
nitrogen boils at -196ºC and both liquid and gas can cause
rapid and severe frostbite. Delicate tissue, e.g. the eyes, can
be damaged by an exposure to the cold gas, which may be too
brief to affect the skin of the hands or face.
PStand clear of boiling and splashing liquid nitrogen and its
gas when filling a dewar at normal room temperature or when
inserting objects (such as a pump) into the liquid.
PUse tongs or wear cryogloves when handling cold or hot
objects. Cryogloves are available as an accessory from Planer
iv

plc. When running a program, the chamber and contents may
get cold enough to cause frostbite.
PEnsure that the delivery pipe connections are secure and
leak-free before supplying liquid nitrogen to the freezer.
PLiquid nitrogen pressure must not exceed 2.0 bar (30 psi).
PTo maintain protection against electric shock, the mains lead
must be properly fitted to a 3-way mains connector plugged
into an earthed mains outlet.
PBecause of the potentially wet laboratory environment, the
operator should be provided with additional protection
against electric shock by supplying mains power to the
freezer through a residual current circuit breaker (RCCB)
operating at a differential of 30 mA. Note that care must be
taken to ensure that the freezer is not compromised by faulty
equipment sharing the same supply and causing nuisance
trips.
PThe 9-way plug on the Controller is only for connecting to
the RS232 serial port of a computer which complies with
IEC950.
PThe AUX socket on the Controller is to be used only for
calibration by qualified service personnel. It must not be
connected to any other equipment.
PThe Sample PRT socket inside the chamber' is only to be
used with a Planer Sample PRT.
PWhen heating under manual control, check the chamber
temperature at intervals of 30 seconds or less to avoid
overheating.
PUser servicing is limited to cleaning. All other servicing
must only be undertaken by suitably qualified engineers.
PTo avoid risk of fire, fuses must always be replaced with the
same type and rating.
v

EMC PRECAUTIONS
The following precautions must be taken to ensure
that the equipment is not damaged by electrostatic
discharge (ESD), and that its immunity to radio
frequency interference is not compromised.
|Ensure that the Controller is not running a program and that the mains
power is disconnected before connecting or disconnecting any cable.
|Fit the plastic covers and plugs provided to all unused connectors.
|Immediately before touching the sample PRT, touch an earthed part,
such as the stainless steel chamber enclosure.
|Do not touch any uncovered connectors with your hands or tools, even
when the equipment is switched off.
|When connecting the system to a PC via the 9 way plug, always use a
fully screened cable no longer than 2m.
|Take care to avoid placing freezers in environments influenced by
sources of electromagnetic interference, such as cyclotrons, large
transformers etc.
vi

Contents
Notices..............................................i
Symbols used in this Manual. . . . . . . . . . . . . . . . . . . . . . . . . . . ii
Symbols used on the Equipment. . . . . . . . . . . . . . . . . . . . . . . ii
SafetyPrecautions................................... iii
FirstAid......................................... iii
Safety........................................... iii
EMCPrecautions................................. iv
Chapter 1 An Introduction to the Kryo1060
AboutthisManual.................................... 1-1
The Kryo1060 Components. . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Symbols on the Kryo1060 Freezer. . . . . . . . . . . . . . . . . . . 1-3
Kryo1060 Chamber Controls. . . . . . . . . . . . . . . . . . . . . . . . 1-3
Controller - Front Controls. . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Controller - Rear Controls. . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
TheKryo1060Freezer............................. 1-6
Personal Computer (PC). . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Printer........................................... 1-8
Chapter 2 Installing the Kryo1060 System
Preparation......................................... 2-1
Unpacking the Equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
InstallingtheFreezer................................. 2-3
Chapter 3 Operating the Kryo1060 System
FreezingSamples....................................3-1
The Procedure to Follow. . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
StoppingaRun................................... 3-3
vii

Shutting Down the System. . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
PowerFailures....................................3-3
Dealing with a System Failure During a Run. . . . . . . . . . . 3-4
RoutineMaintenence................................. 3-5
Disposal............................................ 3-6
Chapter 4 Troubleshooting
Kryo1060-180/380 Freezer. . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Appendix A Kryo1060 Specifications
Kryo1060-380Freezer................................ A-1
Kryo1060-180Freezer................................ A-3
AUXConnector...................................... A-5
Appendix B Spare Parts
OrderingSpares..................................... B-1
Kryo1060Fuses..................................... B-1
viii

C H A P T E R 1
An Introduction to the
Kryo1060
About this Manual
Welcome to the Kryo1060, a system that provides precision
cooling and heating of biological specimens.
This manual is aimed at the Kryo1060-180 and Kryo1060-380
users who are responsible for operating or installing the system.
The manual consists of a number of chapters and appendices:
PThe remainder of this chapter, Chapter 1, gives a description
of each of the major components in the Kryo1060 system.
PChapter 2: Installing the Kryo1060 System is useful if you
are installing the system for the first time.
PChapter 3: Operating the Kryo1060 System describes how to
operate the equipment.
PChapter 4: Troubleshooting is useful if you experience
difficulties with using or setting up the equipment. If you
have a problem, you should always refer to this chapter
before calling your supplier.
PAppendix A: Kryo1060 Specifications gives a full list of the
system specifications, including maximum temperature
ranges, freezing rates, etc.
PAppendix B: Spare Parts gives a listing of Kryo1060 parts,
together with part numbers. Refer to this appendix if you
need to order new parts.
An Introduction to the Kryo1060 1-1

The Kryo1060 Components
The major components of the Kryo1060 system are shown in the
following diagram. The diagram shows the Kryo 1060-380
freezer.
1-2 An Introduction to the Kryo1060
Kryo1060-380 Chamber
PC running DeltaT
Liquid-Nitrogen
Printer
Delivery System Controller

Kryo1060 Chamber Controls
The diagram shows the Kryo1060-380 freezer.
An Introduction to the Kryo1060 1-3
!
!
Solenoid Valve Selector
Valve A Off
!
Valve B
Manual Override Valve
Main Door Handles
Mains On/Off
Controller
Thermocouple
Entry
Port

Controller - Front Controls
1-4 An Introduction to the Kryo1060
-120.4
C
Chamber temperature
(or sample temperature while
sample button is depressed).
EEEE indicates that the chamber
or sample PRT is faulty. A profile
can be run without a working
sample PRT but the chamber
PRT must be in full working
order.
- - - - indicates a stuck button.
S indicates that samples are
ready for seeding if this feature
is selected.
Sample temperature button
Keep depressed to display the
sample temperature.
Indicator light
Lit when running a program.
Flashes for operator attention.
Run
Press to start a profile or after
manual seeding
Stop
Press to stop a profile

Controller - Rear Controls
An Introduction to the Kryo1060 1-5
RS232 Connection to PC
Press to force a reduction in
Press to force an increase in
Auxilliary connector
for service calibration only
Reset the controller
temperature
temperature
Mains inlet
37 Way D Type Connector
for Chamber Connection

The Kryo1060 Freezer
The Kryo1060 freezer has many important design features:
Precision temperature control - Accurate linear cooling and
heating is achieved by the controlled injection of atomised
liquid nitrogen into the chamber and the pulsing of a heater.
A fan system and well-designed ducting ensure uniform
circulation of nitrogen gas past the specimens and a uniform
temperature throughout the freezing chamber.
Precision temperature monitoring - The chamber temperature
is measured by a platinum resistance thermometer (PRT).
Optionally, a second PRT can be used to record the
temperature of the samples. Both of these temperatures can
be displayed as graphs within DeltaT.
Stainless steel construction - The chamber and other parts are
made from high-quality stainless steel for durability and
cleanliness.
Programmable controller - The freezer's operation is
controlled by a microprocessor-based controller. The
controller stores the program that specifies start temperature,
freezing rates, etc. in non-volatile memory. The program can
be retained in memory for several years, even if the freezer's
power is disconnected.
You can load a new program by using the DeltaT software on
a PC.
Stand-alone operation - The freezer can store a program from a
PC and can run that program independently of the PC.
Cool/heat switches - Push-button switches on the rear panel of
the Controller allow manual control of the cooling and
heating functions.
Dual Solenoids - Two solenoid valves are fitted to provide
backup should a failure of one or other occur. A Solenoid
Valve Selection lever on top of the freezer electrically
connects the selected solenoid valve and isolates the faulty
valve from the liquid nitrogen supply.
1-6 An Introduction to the Kryo1060

Manual override valve - In the event of a total control failure, a
manual override valve is provided which can be used to
manually introduce liquid nitrogen into the chamber.
Safety features - The lid activates an interlock switch to switch
off the heater, fan and liquid-nitrogen solenoid valves when it
is opened. Safety from overheating due to sensor failure is
provided by a thermal cutout. Relief valves set to 50psi
(3.4bar) are fitted on the input side of the solenoid valves.
Liquid-Nitrogen Delivery System
It is normal to use a 22psi Dura-Lo or Dura-Tech cylinder to
provide the supply of liquid nitrogen to the freezer. The cylinder
is filled by the supplier and, being self-pressurising, does not
require a pump to deliver the liquid nitrogen to the freezer.
Note The freezer must not be used with supply pressures in
excess of 30psi.
Personal Computer (PC)
The optional DeltaT software on a PC enables you to create and
store profiles. You can run one of these profiles from the PC and
view the recorded chamber and sample temperatures graphically.
All results from the run are stored on the PC for traceability
purposes.
Any profile you run from the PC can be stored in the freezer's
memory. This enables you to rerun the program directly from the
freezer, without having the PC connected.
Printer
If you are using a PC, it is strongly recommended that you
connect a printer. You can use the printer for tasks such as
printing programs and graphs.
An Introduction to the Kryo1060 1-7

1-8 An Introduction to the Kryo1060

C H A P T E R 2
Installing the Kryo1060 System
This chapter describes how to unpack and install the
Kryo1060-380 system.
Preparation
The freezer is intended to be floor mounted on a sturdy surface
able to carry the weight of the freezer easily.
It is important to ensure that there is adequate airflow
around the Kryo1060 for cooling purposes. The
underside of the freezer must be kept clear to ensure
adequate airflow to and from the motors and there must
also be a gap of at least 40mm between the
Kryo1060-380 and any wall or other piece of
equipment.
Ensure that access is provided to the mains inlet at the side of the
freezer.
Power sockets are required for the freezer, chart recorder and PC
(if using DeltaT). Ensure that sufficient power sockets are
nearby.
Unpacking the Equipment
The Kryo1060 is normally packed standing on a wooden pallet.
The freezer itself is provided with castors on its underside which
will permit easy movement into its final location. The freezer
should be positioned as close as possible to its final location
whilst still secured to the pallet. A fork lift truck or similar
handling equipment will be required at this stage.
Installing the Kryo1060 System 2-1

Lifting equipment should only be used by suitably
qualified persons trained in its use. Ensure that the
equipment is suitable for supporting the weight of the
Kryo1060. Refer to local Health and Safety regulations
before proceeding.
1. As described above, position the freezer as close as possible
to its final location whilst still mounted on its pallet.
2. Using a fork lift truck or similar equipment lift the freezer
from the pallet and carefully lower onto the floor.
The legs of the fork lift truck must run through the
channels provided on the underside of the freezer and
protrude past the far end of the freezer before lifting.
NB. These channels are not physically secured to the
freezer body and care must be taken to prevent the
freezer from moving off the channels.
3. From inside the Kryo1060 chamber, remove the following
items:
Operator's Manual
Liquid-nitrogen delivery hose
Mains supply cables
Serial control cable
Sample PRT
Exhaust-duct adapters (1 for the 180; 2 for the 380)
Spare-fuse pack
4. Close the lid and latch it by pushing the two handles
downwards.
5. Wheel the freezer into its final location.
6. Using an 8mm AF spanner, unscrew the feet positioned next
to the two front castors until the feet just begin to take the
load of the freezer and prevent the system from sliding.
2-2 Installing the Kryo1060 System