Sutter Instrument Lambda HPX User manual

Lambda HPX
High-Power LED Light Source System
(Liquid Cooled)
Operation Manual
Rev. 1.03e ((20141208)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax: [phone] Email: [e-mail]

LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)
2
Copyright © 2014 Sutter Instrument Company. All Rights Reserved.

3
DISCLAIMER
The Lambda HPX is a high-power LED illumination or light source device. The purpose of
the system is to be an illuminator for microscopes. No other use is recommended.
This instrument is designed for use in a laboratory environment. It is not intended, nor
should it be used in human experimentation or applied to humans in any way. This is not a
medical device.
Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for
reconfiguration, do not open or attempt to repair the instrument.
Do not allow unauthorized and/or untrained operative to use this device.
Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company
assumes no implied or inferred liability for direct or consequential damages from this
instrument if it is operated or used in any way other than for which it is designed.
SAFETY WARNINGS AND PRECAUTIONS
Electrical
Operate the Lambda HPX using 110 – 240 VAC., 50-60 Hz line voltage. This instrument
is designed for use in a laboratory environment that has low electrical noise and
mechanical vibration. Surge suppression is recommended at all times.
Fuse Replacement: Replace only with the same type and rating:
2 Amp, 250V, 5 x 20mm, Time Delay fuse (EIC 60127-2)
(Example: Bussmann GDC-2A or S506-2A (RoHS))
A spare fuse is located in the power input module.
Avoiding Electrical Shock and Fire-related Injury
Always use the grounded power supply cord set provided to connect the system’s
power adapter to a grounded/earthed outlet (3-prong). This is required to protect you
from injury in the event that an electrical hazard occurs.
Do not disassemble the system. Refer servicing to qualified personnel.
To prevent fire or shock hazard do not expose the unit to rain or moisture.
Electromagnetic Interference
To comply with FDA and CE electromagnetic immunity and interference standards; and to
reduce the electromagnetic coupling between this and other equipment in your lab always
use the type and length of interconnect cables provided with the unit for the interconnection
of its components (see the Technical Specifications appendix for more details).
Operational
Failure to comply with any of the following precautions may damage this device.
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

4
Operate only in a location where there is a free flow of fresh air on all sides.
NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.
This instrument is designed for operation in a laboratory environment (Pollution Degree
I) that is free from mechanical vibrations, electrical noise and transients.
DO NOT CONNECT OR DISCONNECT THE CABLES AND TUBING BETWEEN
THE HPX AND THE HIGH-POWER LED ASSEMBLY WHILE POWER IS ON.
Operate this instrument only according to the instructions included in this manual.
Do not operate if there is any obvious damage to any part of the instrument.
Do not operate this instrument near flammable materials. The use of any hazardous
materials with this instrument is not recommended and, if undertaken, is done so at the
users’ own risk.
Do not operate if there is any obvious damage to any part of the instrument.
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

5
Avoiding Physical Injury while Powered up and Emitting Light
WARNING
DO NOT LOOK DIRECTLY INTO THE OUTPUT OF THE LIGHT
APERTURE OF THE HIGH-POWER LED ASSEMBLY! Always direct
the output of the light aperture into the microscope using the
appropriate adapters, directed away from anyone’s eyes, and not
directed toward any reflective surface.
INFRARED AND ULTRAVIOLET RADIATION: The infrared radiation (and
ultraviolet radiation) generated by this lamp can cause significant skin burns and eye
damage.
Avoiding Physical Injury and Equipment Damage while Using High-Power LED
WARNING
Before removing the High-Power LED Assembly from the HPX
controller, make certain that the system is powered down. To
maximize LED life, run the system for five minutes with the LED off
before disconnecting the cooling hoses.
Other
Retain the original packaging for future transport of the instrument.
Sutter Instrument reserves the right to change specifications without prior notice.
This device is intended only for research purposes.
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

6
(This page intentionally left blank.)
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

7
TABLE OF CONTENTS
DISCLAIMER ..........................................................................................................................................3
SAFETY WARNINGS AND PRECAUTIONS .....................................................................................3
Electrical ..................................................................................................................................................3
Avoiding Electrical Shock and Fire-related Injury..............................................................................3
Electromagnetic Interference ................................................................................................................3
Operational ..............................................................................................................................................3
Avoiding Physical Injury while Powered up and Emitting Light ......................................................5
Avoiding Physical Injury and Equipment Damage while Using High-Power LED ........................5
Other ........................................................................................................................................................5
1. INTRODUCTION ...............................................................................................................................9
1.1 Features Summary ...........................................................................................................................9
1.2 Description.........................................................................................................................................9
1.3 Technical Support...........................................................................................................................10
2. INSTALLATION ...............................................................................................................................11
2.1 Unpacking........................................................................................................................................11
2.2 Installation Instructions ................................................................................................................11
2.3 Configuring Input and Output TTL Logic...................................................................................11
2.3.1 TTL IN to TTL OUT Logic (Switch 1) ..................................................................................12
2.3.2 LED On/Off State Control by TTL IN (Switch 2) ................................................................13
2.3.3 LED On/Off State while Transitioning Between LED Switch Positions ON and TTL
(Switch 3)............................................................................................................................................13
2.3.4 Firmware and Lambda HPX Model (Switch 4) ....................................................................13
3. OPERATIONS...................................................................................................................................15
3.1 Indicators .........................................................................................................................................15
3.1.1 Status Display...........................................................................................................................15
3.1.2 Coolant Level Indicator ...........................................................................................................15
3.2 Controls............................................................................................................................................16
3.2.1 Power Switch ............................................................................................................................16
3.2.2 LED Control Switch.................................................................................................................16
3.2.3 ANALOG-IN Control Switch..................................................................................................16
3.2.4 INTENSITY (PWM) Control Knob .......................................................................................16
3.2.5 CURRENT Control Knob .......................................................................................................16
3.3 Connectors .......................................................................................................................................17
3.3.1 Main LED Connector...............................................................................................................17
3.3.2 Cooling Tube Connectors ........................................................................................................17
3.3.3 ANALOG-IN BNC Connector ................................................................................................17
3.3.4 TTL-IN BNC Connector..........................................................................................................17
3.3.5 TTL-OUT BNC Connector .....................................................................................................17
3.4 Fault Conditions .............................................................................................................................17
4. MAINTENANCE...............................................................................................................................19
4.1 Routine Maintenance .....................................................................................................................19
4.2 Liquid Coolant Replacement .........................................................................................................19
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)
8
APPENDIX A. LIMITED WARRANTY..............................................................................................21
APPENDIX B. ACCESSORIES............................................................................................................23
4.4 High-Power LED Assemblies ........................................................................................................23
4.5 Microscope Adapters.......................................................................................................................23
4.6 TTL Triggering ...............................................................................................................................23
4.7 Liquid Coolant.................................................................................................................................23
APPENDIX C. TECHNICAL SPECIFICATIONS .............................................................................25
APPENDIX D. COOLANT FLUID SAFETY DATA SHEET ...........................................................27
INDEX ....................................................................................................................................................33
TABLE OF FIGURES
Figure 1-1. HPX indicators, controls, and connectors............................................................................9
Figure 2-1. Lambda HPX-series left-side panel removed for access to configuration DIP switches.12
Figure 2-2. Configuration DIP switch bank as viewed head on and top-down.................................. 12
Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda HPX
controller.................................................................................................................................. 15
Figure 3-2. Status display. ...................................................................................................................... 15

9
1. INTRODUCTION
1.1 Features Summary
PRE-FILLED LIQUID COOLING
DIMMING VIA PWM OR CURRENT CONTROL
FRONT MOUNTED CONNNECTORS FOR EASE OF ACCESS
LONG STABLE LAMP LIFE
TTL AND ANALOG EXTERNAL CONTROL
INTEGRAL SHUTTERING
1.2 Description
Figure 1-1. HPX indicators, controls, and connectors.
The Lambda HPX is a liquid-cooled, high-output, LED light source (illuminator). Designed
around a single 90W 4.25mm LED die, the Lambda HPX provides light output comparable to
a 200W xenon arc lamp. The system includes a quiet, vibration-free, liquid cooling head (pre-
filled at the factory) that allows for the low-junction temperatures required to reach the LED
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)
10
bulb manufacturer’s projected life of 60,000 hours. The system has an expected original
output retention rate of 95% at 5,000 hours and 80% at 10,000 hours. The lightweight
precision-machined LED head mounts directly on the epifluorescence port of a microscope
using one of Sutter Instrument’s custom microscope adapters. Because LEDs exhibit color
shift with electrical current change, the Lambda HPX has the ability to dim the LED using
either PWM (pulse width modulation) or current control. The PWM method of dimming is
generally preferred for most applications, and allows the LED to run cooler. For applications
that are intolerant of pulsed output, current control is available instead. Dynamically
updated PWM and Current dimming control status information is displayed on the front
panel; either is manually selectable via control knobs on the front panel. Effective shuttering
is integrated into the system with the very fast on and off (“open” and ”close”) times of 5 and
2.5 microseconds, respectively. TTL input and output allows for software triggering. In
addition to manual control on the front panel, external PWM control is available via analog
input. The LED control/power cable and cooling tubing are easily installed and removed to
and from the controller’s front panel via no-drip quick connectors. Active temperature
monitoring ensures maximized LED life.
Light output is in the visible spectrum from 400 – 729 nm. Both a cool white and “tungsten”
white LEDs are available. Special order units are available with 623, 525, 460, 405, and 385
nm wavelength specific LEDs. A two-channel system with any combination of wavelengths
can be configured by combining a Lambda HPX and Lambda TLED/TLED+.
1.3 Technical Support
Sutter Instrument Company at no charge provides unlimited technical support to our
customers. Our technical support staff is available between the hours of 8:00 AM and 5:00
PM (Pacific Time) at [phone]. You may also e-mail your queries to [e-mail].
Furthermore, as this manual is currently under construction, if there are any areas that you
feel should be covered in detail we would like to hear from you.

11
2. INSTALLATION
2.1 Unpacking
The Lambda HPX and associated hardware comes packed in a single carton. The following is
a list of the components found there. If you believe that any of these components are missing
or show obvious signs of damage from shipping, please contact the factory.
1. Controller cabinet
2. High-Power LED Assembly attached to the controller’s front panel. The assembly
consists of the LED housing to which are attached the high-power control cable for the
LED and two clear tubes containing coolant fluid.
3. Adapter
4. Power Cord
5. Extra cooling fluid
6. Manual
7. One or more additional High-Power LED Assemblies containing different wavelength
LEDs, if ordered.
2.2 Installation Instructions
1. Turn power switch to OFF (“0”) position.
2. Plug power cable into the mains outlet.
3. Connecting the High-Power LED Assembly to the HPX:
a) Connect the power cable of the High-Power LED assembly into the High-Power LED
Connector” on the front panel of the HPX. Position the top of the connector slightly
to the left, gently push inwards toward the connector, and then twist the connector
toward the right (clockwise) until it snaps into position, and then release. Gently pull
on the connector to make sure that it is well seated and connected.
b) Connect one of the cooling tube connectors to one of the Cooling Tube Connectors on
the front panel of the HPX, and then connect the second tube to the other connector.
The fluid connectors are non-specific.
4. The Analog-IN and/or TTL-IN connections can be made to an external signaling source at
stage if desired.
Once all connections have been verified, the system is ready to be turned on.
2.3 Configuring Input and Output TTL Logic
Input and output TTL logic is configured using the first three switches of the four-switch
DIP bank located on the control board inside the controller cabinet. To access the DIP switch
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

12
bank, remove left-side panel (left-side while viewing the front panel) by unscrewing the six
screws.
Figure 2-1. Lambda HPX-series left-side panel removed for access to configuration DIP switches.
While viewing the left side of the cabinet, the location of the DIP switch bank on the
vertically-mounted control board as shown in the following figure.
Figure 2-2. Configuration DIP switch bank as viewed head on and top-down.
The switches on the DIP bank are numbered 1 through 4 left to right. The definitions of
these switches and their positions are described in the following paragraphs and are
summarized in the table that follows.
2.3.1 TTL IN to TTL OUT Logic (Switch 1)
DIP Switch 1 determines the TTL OUT logic as synchronized with TTL IN.
When ON (default), the state of TTL OUT is the same as that of TTL IN
(i.e., when TTL IN goes low, TTL OUT also goes low; when TTL IN goes high, TTL OUT
also goes high).
When OFF, the state of TTL OUT is the inverse of that of TTL IN
(i.e., when TTL IN goes low, TTL OUT then goes high; when TTL IN goes high, TTL OUT
then goes low).
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

13
2.3.2 LED On/Off State Control by TTL IN (Switch 2)
DIP Switch 2 determines how the state at TTL IN determines the on/off state of the lamp
when the LED Control Switch is in the TTL position.
When ON, TTL IN going HIGH turns the LED on; TTL IN going LOW turns the LED off.
When OFF (default), TTL IN going LOW turns the LED on; TTL IN going HIGH turns the
LED off.
2.3.3 LED On/Off State while Transitioning Between LED Switch Positions ON and TTL
(Switch 3)
DIP Switch 3 determines the on/off state of the lamp when switching between manually
adjustable and TTL modes (from the LED switch’s ON or OFF position to TTL).
When ON (default), lamp turns on.
When OFF, lamp turns off.
After switching from manual control to TTL, TTL control of the lamp’s on/off state resumes
on the next transition of logic state at TTL IN.
Note that because the OFF position of the LED switch is located in between ON and TTL,
the lamp turns off while traversing the OFF position when switching from ON to TTL.
2.3.4 Firmware and Lambda HPX Model (Switch 4)
The position of DIP Switch 4 informs the control board’s firmware of the specific Lambda
HPX model it is controlling. This switch must be OFF (Lambda HPX with liquid-cooled
lamp).
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)
1
4
Table 2-1. DIP Switch configuration settings.
Switch Definition Position Definition
ON* Same as TTL IN:
TTL IN=low ÆTTL OUT=low;
TTL IN=high ÆTTL OUT=high
1 TTL OUT logic
OFF Inverse of TTL IN:
TTL IN=low ÆTTL OUT=high;
TTL IN=high ÆTTL OUT=low
ON TTL IN=high turns lamp on2 TTL IN logic
OFF* TTL IN=low turns lamp on
ON* Lamp turns on (Notes 1 and 2)3 Lamp state when switching
from manual control to TTL OFF Lamp turns off (Note 1)
ON Lambda HPX-L54 Lambda HPX model-specific
operation (Note 3) OFF* Lambda HPX
“*” = Default
NOTE 1: After switching from manual mode (LED switch position ON or OFF) to TTL mode
(LED switch position TTL), TTL control of the lamp’s on/off state will resume with the next
change of logic state at TTL IN.
NOTE 2: While using the LED switch to switch from ON to TTL, the lamp turns off while
transitioning through the OFF position (the OFF position is located in between ON and
TTL).
NOTE 3: Switch 4 must be OFF for proper operation of the Lambda HPX (liquid-cooled
lamp).

15
3. OPERATIONS
This chapter describes the operation of the Lambda HPX. The locations of the individual
indicators, controls, and connectors are indicated in the following figure.
Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda HPX controller.
3.1 Indicators
3.1.1 Status Display
Displays intensity as a percentage and current level in terms of amperes.
Intensity = 5%
Current = 18 AMPS
Figure 3-2. Status display.
Intensity ranges from 0 to 100%.
Current ranges from 4 to 18 Amps.
3.1.2 Coolant Level Indicator
This indicator is a liquid-level window through which blue coolant liquid should always be
visible. When the cooling fluid level becomes low, refill to top through the cap on top of the
unit. Additional cooling fluid is included in the shipping box. Additional fluid is available for
purchase from Sutter Instrument.
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

1
6
3.2 Controls
3.2.1 Power Switch
Main power switch turns unit off/on.
3.2.2 LED Control Switch
This is a three-position toggle switch that is used to manually turn the LED on, off, or
activate TTL switching through the TTL-IN BNC connector.
ON: Manually turns on the LED for manual control.
OFF: The intensity of the LED is controlled via voltage range of 0-5 VDC applied to
ANALOG-IN.
TTL: LED on/off state is controlled by TTL triggering (via TTL-IN). Note that remote analog
control (via ANALOG-IN) is turned off when using TTL-IN.
3.2.3 ANALOG-IN Control Switch
Selects between LED dimming control through the ANALOG-IN BNC connector (REMOTE)
or manually using the INTENSITY (PWM) control knob (LOC-1).
REMOTE: Allows for the dimmed state of the LED to be controlled by a voltage (0 – 5 VDC)
applied to ANALOG-IN. The LED Control Switch must be set to OFF in order for dimming
via analog remote input to function properly. When controlling the LED’s dimmed state in
this mode, the status display turns blue.
LOC-1: Allows the INTENSITY (PWM) knob to control the dimmed state of the LED. For the
INTENSITY (PWM) knob to be effective, the LED Control Switch must be set to ON or TTL.
3.2.4 INTENSITY (PWM) Control Knob
Intensity (PWM) Knob: This knob dims the LED via Pulse Width Modulation. In essence, the
LED is being switched at 30KHz. This is the preferable mode of dimming for most
applications, as the LED will run cooler, and exhibit no color shift in the special output as the
light is dimmed. For the INTENSITY (PWM) knob to be effective, the LED Control Switch
must be set to ON or TTL.
The control of the intensity via PWM can also be controlled externally by connecting a 0-5
VDC source to ANALOG-IN connector. This external control is enabled by turning the
ANALOG-IN switch to the LOC-1 position and the LED switch to either ON or TTL. Note
that when PWM intensity control is set up for external control, the INTENSITY (PWM)
knob is disabled.
3.2.5 CURRENT Control Knob
Current Knob: Dims LED by adjusting the current delivered to the LED. Current control
may cause color shift in the LED’s spectral output. However, current-controlled dimming
may be preferable in situations involving the use of ultra high-speed cameras. For the
CURRENT knob to be effective, the LED Control Switch must be set to ON or TTL.
Note that the CURRENT knob is functional regardless of the positions of the LED and
ANALOG-IN switches, and manual or external state of intensity via PWM (manually
adjusting INTENSITY (PWM) knob or applying 0-5 VDC on ANALOG-IN input).
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

17
3.3 Connectors
3.3.1 Main LED Connector
This connector attaches the power leads and sensors for the LED to the main unit. The
connector is a twist connector and must be rotated 20 degrees after plugging in until a snap
is heard. Connector can be released by pulling back on the silver-colored thumb release.
3.3.2 Cooling Tube Connectors
These are the liquid cooling disconnects: These dripless quick disconnects allows removal of
the cooling tubes from the front of the unit. Pulling back on the spring-loaded collar
disconnects the plug and automatically seals the ends of the cooling hose. This allow for ease
of setup and routing of lines through Faraday cages.
CAUTION: THE COOLING HOSES MUST BE CONNECTED BEFORE POWERING
ON UNIT. The cooling tubes are interchangeable, but both must be plugged before
operation. Powering on unit without the cooling tubes attached may damage the fluid pump.
Additionally, powering the LED with the liquid cooling disconnected may burn out the LED.
3.3.3 ANALOG-IN BNC Connector
An analog input from 0-5v controls intensity through PWM dimming.
3.3.4 TTL-IN BNC Connector
Allows for the control of the LED like a shutter. When the LED switch is in the TTL
position, TTL-IN is gated low, which keeps the LED in the OFF state. Once TTL-IN receives
a high signal, the LED turns on for the duration that TTL-IN is held high.
3.3.5 TTL-OUT BNC Connector
Outputs a triggering signal for external software. When the LED is off, TTL-OUT is high;
when the LED is on, TTL-OUT is held low the duration that the LED is on.
3.4 Fault Conditions
The Lambda HPX is equipped with temperature and flow monitors to maximize the life of
the LED. Should a fault occur, the screen turns red and the lamp is turned off automatically.
If the screen turns red, and the text is unchanged, the LED temperature has exceeded it’s
safe operating temperature. If the corner of the screen displays “Pump” or “Fan” when
turning red, it means that the fan or pump has failed, and is no longer turning.
LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)

LAMBDA HPX HIGH-POWER LED LIGHT SOURCE SYSTEM OPERATION MANUAL – REV. 1.03E (20141208)
18
(This page intentionally blank.)