tecan sunrise User manual

Operating Manual for
SUNRISE
Remote Control
Document Part No. I 137 301
July 2001
Document revision : 1.7
Firmware revision level: 3.x

TECAN Affiliates and Service Centers
Austria
Tecan Austria GmbH
Untersbergstrasse 1a
A-5082 Grödig / Salzburg
Austria
Tel.: [phone]
Fax: [phone]
Spain
Tecan Spain
Sabino de Arana, 32
E-08028 Barcelona
Spain
Tel.: [phone]
Fax: [phone]
Belgium
Tecan Benelux B.V.B.A.
Vaartdijk 55
B-2800 Mechelen
Belgium
Tel.: [phone]
Fax. [phone]
Germany
Tecan Deutschland GmbH
Theodor-Strom-Straße 17
D-74564 Crailsheim
Germany
Tel.: [phone]
Fax: [phone]
France
Tecan France S.A.
Parc d'Activités de Pissaloup
Batiment Hermes II
Rue Edouard Branly
F-78190 Trappes
France
Tel.: [phone]
Fax: [phone]
Italy
Tecan Italia S.r.l.
Via F.lli Cervi
Palazzo Bernini
Centro Direzionale Milano2
20090 Segrate (Mi)
Italy
Tel.: [phone]
Fax: [phone]
Japan
Tecan Japan Co. Ltd
Meiji Seimei Fuchu Building 10F
1-40 Miyamachi
Fuchu City, Tokyo
Japan
Tel.: [phone]
Fax: [phone]
Asia
Tecan Asia (Pte) Ltd.
80, Marine Parade
#13-04
Singapore 449269
Singapore
Tel.: [phone]
Fax: [phone]
Netherlands
Tecan Benelux B.V.B.A.
Industrieweg 30,
NL-4283 Giessen,
Netherlands;
Tel.: [phone]
Fax: [phone]
Switzerland
Tecan Schweiz AG
Seestrasse 103
CH-8708 Männedorf
Switzerland
Tel.: + 41 1 922 81 11
Fax. : [phone]
USA
Tecan Boston
200 Boston Avenue
Suite 3000
Medford, MA 02155
USA
Tel.: [phone]
Fax: [phone]
USA
Tecan US
P.O. Box 13953
Research Triangle Park,
NC 27709
USA
Tel.: [phone]
Fax: [phone]
United Kingdom
Tecan UK
Theale Court
11-13 High Street
Theale
UK-Reading RG7 5AH
United Kingdom
Tel.: [phone]
Fax: [phone]
2 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

WARNING
CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED IN
THIS MANUAL BEFORE OPERATING THE INSTRUMENT.
Notice
Every effort has been made to avoid errors in text and diagrams, however,
TECAN Austria Ges.m.b.H. assumes no responsibility for any errors, which may
appear in this publication.
It is the policy of TECAN Austria Ges.m.b.H. to improve products as new
techniques and components become available. TECAN Austria Ges.m.b.H.
therefore reserves the right to change specifications at any time.
We would appreciate any comments on this publication.
TECAN Austria Ges.m.b.H.
Untersbergstraße 1A
A-5082 Grödig/Salzburg
AUSTRIA / EUROPE
Telephone: [phone]
FAX: [phone]
E-mail: [email protected]
Copyright Information
The contents of this manual are the property of TECAN Austria Ges.m.b.H and
are not to be copied, reproduced or transferred to another person or persons
without our prior written permission.
Copyright TECAN Austria Ges.m.b.H
All rights reserved.
Printed in Austria.
Declaration for EU Certificate
See the back of this manual.
About this Manual
This manual describes the basic SUNRISE Remote Control, designed to
measure the light absorbency (optical density) of samples in 96 well microplates.
It is intended as a reference and instruction manual for the user.
This manual instructs how to:
• Install the instrument
• Clean and maintain the instrument
Warranty
3 Year Warranty
As an expert for microplate instrumentation TECAN proves its commitment to
quality and offers a unique 3 year warranty for your SUNRISE reader as a
standard.
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 3

Warnings, Cautions and Notes
There are three types of informational notices used in this manual. These notices
highlight important information or warn the user of a potentially dangerous
situation. The following notices are:
Note: Gives helpful information.
Caution
Indicates a possibility of instrument damage or data loss if instructions are
not followed.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF
LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT
FOLLOWED.
4 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

Table of Contents
1. Safety
1.1 Instrument Safety ......................................................................1-1
2. General
2.1 Introduction................................................................................2-1
2.1.1 Available Options for SUNRISE ..................................................2-2
2.2 Specifications ............................................................................2-3
2.2.1 Barcode Laser Scanner Specifications........................................2-5
2.3 Instrument Description .............................................................2-6
2.4 Back Panel Connections...........................................................2-7
2.5 Filter Carriage Description........................................................2-8
2.5.1 SUNRISE Gradient Filter Carriage ..............................................2-8
2.5.2 SUNRISE Standard Filter Carriage .............................................2-8
2.6 Software Features .....................................................................2-9
2.7 Instrument Features ..................................................................2-9
2.7.1 Measurement Modes ...................................................................2-9
2.7.2 Microplate Shaking ....................................................................2-10
2.8 Instrument Accessories ..........................................................2-10
Computer Software for Personal Computer...............................2-10
3. Installation Procedure
3.1 Introduction................................................................................3-1
3.2 Unpacking and Inspection........................................................3-1
3.3 Unpacking Procedure ...............................................................3-2
3.4 Power Requirements.................................................................3-2
3.5 Environmental Requirements...................................................3-2
3.6 Instrument Installation Procedure ...........................................3-3
3.7 Installation of Instrument Control Software............................3-3
3.8 Define Filter................................................................................3-4
3.9 Defining the SUNRISE Instrument Settings ............................3-5
3.9.1 Installation of SUNRISE Instrument Settings Software ...............3-5
3.9.2 Starting the SUNRISE Instrument Settings .................................3-5
3.9.3 Define Instrument Mode ..............................................................3-6
3.9.4 Define Filter .................................................................................3-6
3.9.5 Define Measurement Mode .........................................................3-7
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 5

4. Error Messages and Trouble Shooting
4.1 Introduction ............................................................................... 4-1
4.1.1 Table of Error Messages
and Trouble Shooting for SUNRISE Mode................................. 4-1
4.1.2 Table of Error Messages
and Trouble Shooting for SPECTRA Mode................................ 4-4
5. Maintenance & Cleaning
5.1 Introduction ............................................................................... 5-1
5.2 Lamp Replacement ................................................................... 5-1
5.3 Replacement of Filters in Standard Filter Carriages.............. 5-3
5.4 Filter Replacement for Gradient Filter..................................... 5-4
5.5 Fuse Replacement..................................................................... 5-4
5.6 Cleaning the Instrument ........................................................... 5-5
Liquid Spills ................................................................................. 5-5
5.7 Instrument Disinfection ............................................................ 5-5
5.7.1 Disinfection Procedure ................................................................ 5-5
5.8 Disinfection Certificate ............................................................. 5-6
5.9 Disposal of Instrument ............................................................. 5-7
5.10 Preventive Maintenance Plan for SUNRISE ............................ 5-7
5.10.1 Daily ............................................................................................ 5-7
5.10.2 Weekly ........................................................................................ 5-7
5.10.3 Every Six Months ........................................................................ 5-7
5.10.4 Yearly (Service Engineer Required)............................................ 5-7
5.10.5 Every Four Years ........................................................................ 5-7
6. Performance Testing / Quality Control
6.1 Introduction ............................................................................... 6-1
6.2 Operating for Maximum Performance ..................................... 6-1
6.2.1 Instrument Location..................................................................... 6-1
6.2.2 Operating Procedure................................................................... 6-2
6.2.3 Self Check Procedure ................................................................. 6-2
6.3 Performance Tests.................................................................... 6-3
6.3.1 QC PAC 2.................................................................................... 6-3
6.3.2 Microplate Test............................................................................ 6-3
6.3.3 High Meniscus Liquids ................................................................ 6-5
Agglutination Method .................................................................. 6-5
Manual Method............................................................................ 6-6
6.4 Quality Control Testing............................................................. 6-7
6.4.1 Precision Testing......................................................................... 6-7
6.4.2 Instrument Accuracy.................................................................... 6-8
6.4.3 Instrument Linearity..................................................................... 6-9
Index
6 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

1. Safety
1. Safety
1.1 Instrument Safety
1. Always follow basic safety precautions when using this product to reduce the
risk of injury, fire or electrical shock.
2. Read and understand all information in the Operating Manual. Failure to
read, understand and follow the instructions in the manual may result in
damage to the product, injury to operating personnel or poor instrument
performance.
3. Observe all WARNINGS and CAUTION statements in the manual.
4. Never open SUNRISE while the instrument is plugged into a power source.
5. Observe proper laboratory safety precautions, such as wearing protective
clothing and using approved laboratory safety procedures.
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 1-1

2. General
2. General
2.1 Introduction
SUNRISE Remote Control is intended for use with only external software.
The SUNRISE instruments are fully automatic, microprocessor controlled readers
designed for professional use, enabling the user to measure the light absorbency
(optical density) of samples in 96 well microplates according to the specifications
described in this manual for in vitro diagnostics.
Results obtained using the SUNRISE are influenced by the proper use of
the instrument, according to the instructions given in this manual, as well
as the liquid compounds used (reagents, chemistry). The instructions for
use, storage and other manipulations in connection with samples or
reagents have to be strictly followed. Taking this fact into consideration,
results must be interpreted carefully.
By reading twelve wells simultaneously, the instrument is able to measure a
microplate in approximately six seconds.
The obtained transmission values are converted into OD values according to the
following formula:
T
1
LogOD =
lightdetectedI
lightemittedIo
=
=
I
I
TonTransmissi
o
=
With an innovative range of options, this versatile MTP reader gives diagnostic
and research laboratories all the features for numerous purposes.
Based on a new design concept EPAC, the excellent optical performance and
high quality of the SUNRISE will guarantee fast, reproducible and accurate
measurements.
The SUNRISE is designed to be fitted into TECAN robotic systems.
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 2-1

2. General
2.1.1 Available Options for SUNRISE
The Sunrise is a modular system, so you can create your own tailor-made
instrument that meets exactly your needs. Options such as a touch screen
combined with a WindowsCE based on-board software, free wavelength
selection, temperature control and a bar code scanner can be added onto the
basic system which is dedicated to remote controlled computer operation.
Touchscreen
Tuneable wavelength
from 400 – 700 nm
Temperature control
Barcode scanner
SUNRISE
Remote Controlled
B037302
B037306
B037303
B037304
Caution
If the instructions given in this manual are not correctly performed, the
instrument may be damaged or the procedure may not be correctly
performed and the safety of the instrument cannot be guaranteed.
For more information about the operating instructions, see Magellan
or XRead Plus manuals.
2-2 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

2. General
2.2 Specifications
The table below lists the specifications for the instruments.
The gradient filter slide can be used if the option tuneable wavelength is
available.
PARAMETERS CHARACTERISTICS
Measurement time:
dual wavelength
single wavelength
8 seconds
6 seconds
Wavelength Range
Standard
Gradient
340 - 750 nm
340 - 399 nm & 700 - 750 nm using
Standard filter carriages
400 - 700 nm using gradient filter
Measurement Range:
340 – 399 nm
400 – 750 nm
340 – 399 nm
400 – 750 nm
0 - 3.000 Abs.
0 - 4.000 Abs.
0 - 3.000 Abs.
0 - 4.000 Abs.
Indication Range:
340 – 399 nm
400 – 750 nm
0 - 3.000 Abs
0 - 4.000 Abs
Resolution: 0.001 Abs.
Accuracy: Using standard filter
(492 nm) 0.000 - 2.000 Abs
(492 nm) 2.000 - 3.000 Abs
Using gradient filter
(492 nm) 0.000 - 2.000 Abs
Better than +/- 1.0 % +/- 0.010 Abs.
Better than +/- 1.5 % +/- 0.010 Abs.
Better than +/- 1.5 % +/- 0.010 Abs.
Precision: Using standard filter
(492 nm) 0.000 - 2.000 Abs.
(492 nm) 2.000 - 3.000 Abs.
Using gradient filter
(492 nm) 0.000 - 2.500 Abs
Better than +/- 0.5 % +/- 0.005 Abs
Better than +/- 1.0 % +/- 0.005 Abs
Better than +/- 1.0 % +/- 0.005 Abs
Linearity: Using standard filter
(340 - 399 nm) 0.000 - 2.000 Abs.
(400 - 750 nm) 0.000 - 2.000 Abs.
2.000 - 3.000 Abs.
Using gradient filter
(492 nm) 0.000 - 2.500 Abs.
Better than +/- 2 %
Better than +/- 1 %
Better than +/- 1.5 %
Better than +/- 2 %
Stability:
Normal measurements
After 15 minutes warm up time
max. +/- 0.001 Abs
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 2-3

2. General
PARAMETERS CHARACTERISTICS
Wavelength selection:
Gradient filter
Standard Filter
Special gradient filter any wavelength
between 400 and 700 nm in 1 nm steps.
Narrow band interference filters. Up to
four filters can be mounted in a filter
carriage. The instrument can use up to
eight different filter carriages (including
the Gradient filter).
Light source: Halogen lamp 20 W
All connected devices must
be approved and listed as per
EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data
Processing Devices.
Computer interface:
Serial RS 232 C
Printer
300 - 38,400 baud
Only functions with the touch screen
option.
Mains Power Input 100 - 120 & 220 - 240 V ± 10% 50/60 Hz
(Auto sensing)
Consumption Stand by mode: approx. 50VA
Operational mode: max.110VA
Fuse Rating 2 x F 2.0 A / 250 V (Fast Blow)
Outside Dimensions Width: 33,5 cm
Depth: 32,0 cm
Height: 17,0 cm
Weight 6 kg
Ambient temperature:
Operation 15°C to 35°C (59°F to 95°F)
Non-Operation Below -10°C (14°F)
Above 43°C (109°F)
Storage -40°C to 60°C (-40°F to 140°F)
Relative Humidity:
Operation 20 % to 90 %
Non-Operation Below 5 %
Above 95 %
Storage 5 % to 95 %
Overvoltage Category II
Pollution Degree 2
Method of disposal Contaminated waste
2-4 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

2. General
2.2.1 Barcode Laser Scanner Specifications
WARNING
LASER RADIATION – DO NOT STARE INTO THE BEAM!
CLASS II LASER PRODUCT.
The Class II Laser Scanner corresponds to the following norms:
• DIN EN 60825-1 : 1997 – 03
• CDRH 21 CFR 1040.10
PARAMETERS CHARACTERISTICS
Classification Class II Laser Product
Input Power 5V DC +/- 10%
Emission Duration > 0.25s
Scan Rate 42 +/- 3 Scans / second (bi-directional)
Laser Power < 1mW
Definition of depth Max. 40cm
Resolution 0.15cm
Min. Print Contrast 25% at 675nm
Ambient Light Sun Light: 40000 Lux
Halogen Light: 1500 Lux
Label Reproductions on the Instrument Casing and Interior:
LASER RADIATION -
DO NOT STARE INTO BEAM
CLASS II LASER PRODUCT
Complies with 21CFR
and 1040.10 and 1040.11
CAUTION
LASERSTRAHLUNG
NICHT IN DEN STRAHL BLICKEN
LASER KLASSE 2
Leistung: <1mW
Wellenlänge: 675nm
EN60825-1:1994+A11:1996
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 2-5

2. General
2.3 Instrument Description
The illustration below shows the components of the instruments.
Power ON LED
Lamp Compartment
Plate Support Cartridge and Filter Block Compartment
2-6 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

2. General
2.4 Back Panel Connections
The illustration below shows the connections located in the back panel of the
instrument.
TECAN
A-5082 AUSTRIA
MADE IN
AUSTRIA
TECAN
50 / 60
TYPE
ART.-NR.
SER.-NR.
VOLTAGE
M-CODE
VA
Hz
AC 100 - 120 / 220 - 240
Sunrise
F 0 3 9 3 x x 1 1 0
xxxxx
xxxxxx
2 x F2A
U
L
RU
L
R
C
LISTED
8C64
LABORATORY EQUIPMENT
TECAN
RS 232
PRINTER
LASERSTRAHLUNG
NICHT IN DEN STRAHL BLICKEN
LASER KLASSE 2
Leistung: <1mW
Wellenlänge: 675nm
EN60825-1:1994+A11:1996
I
0
Instruments Main Fuses
ON / OFF Switch
Mains Socket
Printer Connector Serial RS 232 Connector
( Only functions with
the touch screen option )
BarCode Laser Scanner labels
( only with the barcode option )
LASER RADIATION -
DO NOT STARE INTO BEAM
CLASS II LASER PRODUCT
Complies with 21CFR
and 1040.10 and 1040.11
CAUTION
All connected devices must be approved and listed as per EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data Processing Devices
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 2-7

2. General
2.5 Filter Carriage Description
The SUNRISE instrument can use the following types of filter carriages:
SUNRISE Standard and SUNRISE Gradient Filter (with tuneable wavelength
option).
2.5.1 SUNRISE Gradient Filter Carriage
The SUNRISE Gradient filter carriage is fitted with a gradient filter that allows the
selection of any wavelength from 400 to 700 nm.
Gradient Filter Carriage
Gradient Filter
700 nm 400 nm
The Gradient filter carriages are calibrated by the manufacturer and each one is
unique.
When another Gradient filter is inserted into the instrument, the instrument
must be re-calibrated. This re-calibration procedure can only be performed
by the manufacturer or by an service engineer.
When a wavelength is selected, the entered wavelength is compared against the
calibration table.
The filter carriage is moved the required distance, so that the required section of
the gradient filter is in the light beam.
2.5.2 SUNRISE Standard Filter Carriage
The SUNRISE standard filter carriage is fitted with up to four narrow band
interference filters which have a fixed wavelength.
SUNRISE Standard Filter Carriage
When a wavelength is selected, the entered wavelength is compared against the
list of entered filter values for this filter carriage.
If the required filter is fitted in the filter carriage, the filter carriage is moved so
that the required filter is in the light beam.
For more information about the definition of the new and customized filter
slides, see 3.8 Define Filter.
2-8 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

2. General
2.6 Software Features
SUNRISE Remote Control is intended for use with only external software.
For more information about the software features, see the appropriate
individual manuals. For example: refer to the Magellan Reference manual.
2.7 Instrument Features
Microplates can be measured using the following features:
• Various measurement modes
• Single or dual wavelength measurements
• Microplate shaking
2.7.1 Measurement Modes
The instrument can be set to use the following measurement modes:
Fast Plate transport is moved quickly under the measurement diodes so
that a fast measurement is obtained. (Default setting).
Accurate Plate transport is moved slowly under the measurement diodes so
that a very accurate measurement is obtained.
Center This option measures the optical density only at the center of the
well.
With the Fast and Accurate measurement modes, the optical density is measured
at three positions across the wells and the average measured optical density
value from the three measurements is used as the optical density of the well.
The accurate measurement cycle should always be used when measuring
high optical densities.
The Center measurement mode should be used if the liquid in the microplate
produces a high meniscus, as an incorrect optical density could be obtained if the
optical density is measured at three positions. If an Agglutination measurement is
performed, all the measurement positions are used.
For more information about setting the measurement mode,
see 3.9 Defining the SUNRISE Instrument Settings.
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 2-9

2. General
2.7.2 Microplate Shaking
The SUNRISE is able to shake the microplate before it is measured. Use external
software (for example: Magellan) to set the shaking modes.
The microplate can also be shaken between each of the kinetic measurement
cycles.
When using a 96 well plate, spillage may occur if the wells are filled with more
than 300µl, while using high shaking mode.
2.8 Instrument Accessories
The table below contains the order numbers for instrument accessories:
Part Name Part Order Number
Halogen lamp. ................................................................................ 3 709 008
Reader to external computer cable................................................. 335 0005
QC Pac 2 for SUNRISE and SPECTRA ........................................B 037 358
Additional filter slide .......................................................................B 036 301
Computer Software for Personal Computer
Related Software
Part of Reader PC Package:
Software Functionality
Magellan Instrument control and data reduction
XRead Plus Instrument control and transfer of raw data to
Excel.
SUNRISE instrument setting Enables settings of SUNRISE instrument
(SUNRISE, SPECTRA, ATC mode and so on).
RDR download Enables download of new firmware from PC to
reader.
SUNRISE Error Diagnosis Creates printout of instrument status for service
purposes.
2-10 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 July 2001

3. Installation Procedure
3. Installation Procedure
3.1 Introduction
This chapter contains the necessary information for installing the instrument.
The installation procedures involve unpacking, environmental requirements,
power requirements and interfacing.
3.2 Unpacking and Inspection
The delivered instrument is shipped in one carton, which includes:
• Power cable
• Computer connection cable
• SUNRISE Operating manual, XRead Plus manual
• Spare fuses
• A software CD, which also contains the XRead Plus program and Magellan
demo program (30 day working license).
Accessory box
Foam Packaging Foam
Packaging
Instrument
July 2001 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.7 3-1

3. Installation Procedure
3.3 Unpacking Procedure
1. Visually inspect the container for damage, before opening it.
Report any damage immediately.
2. Place the carton in an upright position and open it.
The cartridge, filter block and plate transport compartments are fixed with
adhesive tape. The location of the adhesive tape is indicated with a red
arrow.
3. Lift the instrument out of the carton and place it on a flat surface, free from
dust, vibration and away from direct sunlight.
4. Visually inspect the instrument for loose, bent or broken parts.
Report any damage immediately.
5. Compare the instrument's serial number, attached on the rear panel of the
instrument, against the serial number of the instrument, on the delivery
(shipping) note.
6. Check the instrument accessories against the delivery (shipping) note.
7. Open the plate support area cover and remove the foam strip that is used as
the microplate transport lock.
8. Please save all packing materials, as it may be required for later
transportation.
3.4 Power Requirements
The instrument is auto sensing for the supplied voltage, and therefore does not
have to be set for the correct voltage.
Connect the instrument only to a electricity supply system with protective earth.
WARNING
TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE
REPLACED WITH THE SAME TYPE AND RATING OF FUSES.
3.5 Environmental Requirements
The instrument should be placed on a flat, level surface that is free from dust,
solvents and acidic vapors.
Vibration and direct sunlight must be avoided, to ensure correct results.
3-2 Operating Manual for SUNRISE Remote Control No: I 137 301 Rev No: 1.6 July 2001