Thermo Scientific CW3 User manual

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Thermo Scientific CW3 Cell Washer
Instruction Manual
50144259_h • 06 / 2018

Thermo Scientic CW3 |3
Table of Contents
1. Preface ............................................................... 5
1. 1. Items Supplied .............................................................5
1. 2. Intended Use...............................................................7
1. 3. Precautions ................................................................7
1. 4. Symbols Used on the Cell Washer .............................................10
1. 5. Symbols Used in the Manual .................................................11
2. Technical Specifications ................................................ 12
2. 1. Directives and Standards ....................................................13
2. 2. Mains Supply .............................................................13
2. 3. Location and Function of Parts................................................14
2. 4. Control Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2. 5. Condition-setting Panel .....................................................18
3. Transport and Set Up ................................................... 20
3. 1. Before Setting Up ..........................................................20
3. 2. Location .................................................................20
3. 3. Transporting ..............................................................20
3. 4. Leveling..................................................................21
3. 5. Check Mains Connection ....................................................21
3. 6. Setting Up ................................................................21
3. 7. Storage ..................................................................32
3. 8. Shipping .................................................................32
4. Operation ............................................................ 33
4. 1. Preparation ...............................................................33
4. 1. 1. Injection Volume Adjustment and Pump Ventilation..........................................33
4. 1. 2. Setting the Operating Conditions .......................................................35
4. 1. 3. Preparation of the Sample............................................................35
4. 2. Operation Modes...........................................................36

4|CW3 Thermo Scientic
4. 2. 1. Automatic Mode ...................................................................36
4. 2. 2. Manual Mode .....................................................................36
4. 2. 3. Agitation-Centrifugation..............................................................36
4. 2. 4. Operational Sequence for Antiglobulin Test................................................37
5. Maintenance .......................................................... 38
5. 1. Cleaning .................................................................38
5. 1. 1. Pump, Tank and Tubing..............................................................39
5. 1. 2. Rotor, Distributor and Bowl ...........................................................39
5. 1. 3. Chamber, Splash Guard, Drain Cover and Door Stopper Parts..................................40
5. 2. Preventive Maintenance .....................................................41
5. 3. Shipping and Disposal ......................................................41
6. Troubleshooting ....................................................... 42
6. 1. Mechanical Emergency Door Release ..........................................42
6. 2. Error Codes ...............................................................43
6. 3. Troublshooting when no Error Code is indicated ..................................44
Decontamination Information Certificate ..................................... 45
Chemical Compatibility ................................................... 46
Index .................................................................. 49

Items Supplied
Thermo Scientic CW3 |5
1. Preface
1. 1. Items Supplied
Article No. Description Quantity
Thermo Scientific CW3 cell washer 1
S402776A Bowl Assembly 1
S413259C Tank (5 L) 1
4744346 Tubing 3 m
75000015 Tube Connector 1
S4011034 Drain Tube 2.5 m
480270 Test Tubes (12 mm in diameter and 75
mm in length) 50 (1 box)
483719 Grease 1
8046004 Hex. Wrench 1
S411107 Motor Guard Plate 1
4666354
4666357
4666355
Hose Band
Small
Medium
Big
1
1
2
S413181A L-shaped Adapter 1
480879 D10 Adapter 25
Power Cord 1
Instruction Manual 1 
Rotor Assembly Options 1
75000020 24 place rotor assembly with distributor
75000021 12 place rotor assembly with distributor

Items Supplied
6|CW3 Thermo Scientic
Article No. Description Quantity
Replacement Parts
Rotor Assembly 1
75000022 24 places
75000023 12 places
Distributor Assembly 1
75000024 24 places
75000025 12 places

Intended Use
Thermo Scientic CW3 |7
1. 2. Intended Use
The Thermo Scientific CW3 cell washer is designed to perform cell washing in multiple washing cycles using saline
solution. The cell washer provides blood cells after sample separation, which can be used for further blood testing such
as Antiglobulin test, ABO compatibility, Rh testing, Cross-matching and Antibody screening.
The centrifuge should always be operated by a trained individual such as a clinical laboratory technologist or a person
with a similar education.
1. 3. Precautions
Carefully read the following safety precautions for a thorough understanding.
Protection is impaired if the cell washer is not used as specified in the intended use.
Follow the instructions and procedures described in this instruction manual to operate this cell washer safely.
Be sure to observe the precautions in this instruction manual and on your equipment.
It is the general obligation of the operator to make sure, that the proper protective clothing is used. Mind the „Laboratory
Biosafety Manual“ of the World Health Organization (WHO) and the regulations in your country.
Safety messages are indicated as follows. They are stated with signal words combined with the safety alert symbol to call
your attention to items or operations that could be dangerous to you or other persons using this equipment. The definitions
of signal words are as follows:
WARNING: Warning notes indicate any condition or practice, which if not strictly observed, could result in seri-
ous injury or possible death.
CAUTION: Caution notes indicate any condition or practice, which if not strictly observed or stopped, could
result in personal injury, damage or destruction of the equipment.
NOTICE: Notes indicate an area or subject of special merit, emphasizing either the product’s capabilities or
common errors in operation or maintenance.
Do not operate this cell washer in any manner not described in this Instruction Manual. Should you have any troubles with
this cell washer, call a Thermo Fisher Scientific authorized sales/service representative.
The precautions described in this Instruction Manual are carefully developed to cover all possible risks. It is important that
you are alert for unexpected incidents. Be careful when operating this cell washer.

Precautions
8|CW3 Thermo Scientic
WARNING
1. The cell washer is neither inert nor protected against explosion. Never use the cell washer in an explosion-prone
environment.
2. Do not set up the cell washer in or near places where flammable gases are generated or chemicals are stored.
3. If centrifuging any hazardous materials mind the „Laboratory Biosafety Manual“ of the World Health Organization
(WHO). Look on the internet page of the World Health Organization (www.who.int) for the „Laboratory Biosafety
Manual“.
For materials in a higher risk group, extra safety measures have to be taken.
4. Take all necessary safety measures before using samples that are toxic or radioactive, or blood samples that are
pathogenic or infectious. You use such samples at your own responsibility.
a. If the cell washer, rotor, or an accessory is contaminated by samples that are toxic or radioactive, or blood
samples that are pathogenic or infectious, be sure to decontaminate the item according to good laboratory
procedures and methods.
b. If there is a possibility that the cell washer, rotor, or an accessory is contaminated by samples that might
impair human health (for example, samples that are toxic or radioactive, or blood samples that are
pathogenic or infectious), it is your responsibility to sterilize or decontaminate the cell washer, rotor, or
the accessory properly before requesting repairs from a Thermo Fisher Scientific authorized sales/service
representative.
c. It is your responsibility to sterilize and/or decontaminate the cell washer, rotor, or parts properly before
returning them to a Thermo Fisher Scientific authorized sales/service representative.
5. Do not centrifuge explosive or flammable materials or substances which could react violently
6. Because the liquid samples, the saline, etc. are used in this cell washer, your cell washer must be grounded
properly.
7. To avoid electrical shocks, do not handle the power cord or turn on or off the POWER switch with wet hands.
8. The cell washer itself may move if the rotor fails during high-speed rotation. Make sure there is a 30 cm area
around the cell washer that will allow for such movement and do not allow individuals to enter into that area
during operation. Also do not place dangerous objects such as flammable or explosive materials on top of the
cell washer or in the surrounding area.
9. While the rotor spins, never unlock the door.
10. Repairs, disassembly, and other modifications to the cell washer are strictly prohibited unless performed by a
Thermo Fisher Scientific authorized sales/service representative.

Precautions
Thermo Scientic CW3 |9
CAUTION
1. Turn off the cell washer at the main switch. The mains plug must be freely accessible at all times.
Press STOP to shut the cell washer down.
Pull out the power supply plug or disconnect the power supply in an emergency.
2. Use the cell washer only with correct installed rotor and distributor assembly and correct inserted bowl, splash
guard and drain cover.
3. Do not move or relocate the cell washer while the rotor spins.
4. Do not lean on the cell washer.
5. Do not pour any solution such as water, detergent, or disinfectant directly into the rotor chamber. If you do so,
the bearings of the drive unit might corrode or deteriorate.
6. Connect the saline tank to the pump inlet connector of the cell washer correctly with the hose bands (standard
accessories). Otherwise the liquid might leak from the tube and the liquid might get into the inside of the cell
washer. If you think liquid is inside of the cell washer, contact a Thermo Fisher Scientific authorized sales/service
representative to clean and dry the cell washer.
7. Before you operate the cell washer, remove any dropped objects and tube fragments from inside the rotor
chamber.
8. Always check for corrosion and damage on the rotor before using it. Do not use a corroded or damaged rotor.
9. Be sure to use the specified test tubes with a wall thickness of 1 mm or more. When using thin test tubes such
as disposable test tubes, do not use them repeatedly. If any scratches, crack, inside warp, etc. is found on the
test tubes, do not use them because they cannot bear a centrifugal force.
10. Be sure to load test tubes to all the holders.
11. Use one or two drops (about 50 μl) of 3-5% erythrocyte suspension as sample volume for blood cell washing.
When using precipitated erythrocyte layer, use one or two drops (about 50 μl or less) per test tube. Use the 80%
or less of the test tube capacity as sample volume for centrifugation.
12. If you observe some abnormality in this product, stop using it immediately and contact a Thermo Fisher Scientific
authorized sales/service representative. Notify the service representative of the alarm code if displayed.
13. If the cell washer will not be used for a long time, remove the power cord from the socket.
14. For connection to a different outlet, the power cord might be needed to be replaced.
15. Follow local electrical codes.
16. Depending on the magnitude, an earthquake might damage the cell washer.
If you observe some abnormality, contact a Thermo Fisher Scientific authorized sales/service representative.
NOTICE
Usually the control panel and the surface of the cell washer get warm during operation.