ThermoFisher Scientific Invitrogen iBright CL1000 User manual
For Research Use Only. Not for use in diagnostic procedures.
iBright™Imaging Systems
USER GUIDE
For use with the iBright™CL1000 Imaging System and the iBright™
FL1000 Imaging System
Catalog Numbers A32749 (CL1000) and A32752 (FL1000)
Publication Number MAN0015702
Revision A.0
Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL,
INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR
USE OF IT.
Revision history: Pub. No. MAN0015702
Revision Date Description
A.0 May 2017 New document. Describes installation, operation, and maintenance of the
iBright™Imaging Systems
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2017 Thermo Fisher Scientific Inc. All rights reserved.
Contents
■Product information ....................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 7
■Start, sign on, and configure the instrument ............................ 8
Create an instrument profile ..................................................... 8
Link a new profile to your Thermo Fisher Cloud account ............................. 8
Link an existing profile to your Thermo Fisher Cloud account ......................... 9
If the unit is off .................................................................. 9
If the unit is on and in active mode ................................................ 10
■Operate the iBright™imager ............................................ 11
Choose imaging mode and start image capture .................................... 11
Chemi Blots ................................................................... 12
Capture an image of the sample ............................................. 12
Fluorescent Blots (FL1000 model only) ........................................... 13
Image using Fluorescent Blots (FL1000 model only) ............................ 13
Select a dye in the Fluorescent Blots mode .................................... 13
Edit a dye in the Fluorescent Blots mode ...................................... 14
Edit dye channel exposure time .............................................. 14
Nucleic Acid Gels .............................................................. 15
Image using Nucleic Acid Gel ................................................ 15
Protein Gels ................................................................... 16
Image using Protein Gel .................................................... 16
Options available in modes ...................................................... 17
Image Gallery ................................................................. 20
View images .............................................................. 20
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■Export and analyze images .............................................. 21
Export images ................................................................. 21
Export new images ........................................................ 21
Export gallery images ...................................................... 21
Select an Export file type ................................................... 22
Analyze images ................................................................ 23
Analyze new images ....................................................... 23
Analyze gallery images ..................................................... 24
■Maintain the instrument ................................................. 26
Settings ....................................................................... 26
Manage accounts .......................................................... 27
Run instrument diagnostics ..................................................... 28
Clean the instrument ........................................................... 28
Replace the instrument fuses .................................................... 29
Materials required ......................................................... 29
Replace the fuses .......................................................... 29
Move the instrument ........................................................... 30
Prepare for shipping ....................................................... 30
■APPENDIX A Instrument specifications .............................. 31
Instrument dimensions and specifications ......................................... 31
Electrical requirements ......................................................... 31
Environmental requirements .................................................... 32
Network requirements .......................................................... 33
Instrument and computer connections ............................................ 34
■APPENDIX B Safety ..................................................... 36
Symbols on this instrument ..................................................... 36
Safety alerts on this instrument ............................................. 37
Location of safety labels on the instrument ................................... 38
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 38
Instrument safety .............................................................. 38
General .................................................................. 38
Physical injury ............................................................ 38
LED (Light-Emitting Diode) .................................................. 39
Electrical ................................................................. 39
Contents
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Cleaning and decontamination ............................................... 40
Instrument component and accessory disposal ................................ 40
Safety and electromagnetic compatibility (EMC) standards .......................... 41
Safety compliance ......................................................... 41
EMC ...................................................................... 41
Environmental design ...................................................... 42
Chemical safety ................................................................ 43
Biological hazard safety ......................................................... 44
■Documentation and support ............................................. 45
Related documentation ......................................................... 45
Customer and technical support ................................................. 45
Limited product warranty ....................................................... 46
Contents
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Product information
IMPORTANT! Before using this product, read and understand the information in the
“Safety” appendix in this document.
Product description
The iBright™ Imaging Systems allow users to image a variety of blots and gels. The
iBright™ CL1000 Imaging System provides for imaging of chemiluminescent Western
blots, DNA and RNA gels stained with uorescent nucleic acid stains, and visible
protein gels. The iBright™ FL1000 Imaging System adds the capability of imaging
uorescent Western blots using multiple dyes.
The iBright™ imagers use a simple design and intuitive workows to deliver high-
resolution images. The instrument can be run directly from the touchscreen to start
and create images, while also providing on-board software for image analysis. To
further analyze data, the instrument can be integrated with the iBright™ Image
Analysis Software available through your Thermo Fisher Cloud account.
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Parts of the instrument include:
1
2
3
1Touchscreen — Controls the instrument.
2USB port — For connection to an external network drive, jump drive, or other external
data storage device.
3Sample stage/turntable — Contains turntable for sample placement.
Contents
Table 1 Included imager system parts
Component Quantity
Power cord (region specific) 1
Quick reference card 1
White transilluminator screen 1
Reference target 1
Sample stage/turntable 1
Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage/turntable A33829
High-Power USB Wi-Fi Module A26774
Product information
Contents
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Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Enter a Screen name.
4. Enter and conrm a four-digit pin.
5. Touch Create prole.
6. (Optional) If you want to link your instrument to your Thermo Fisher Cloud
account, touch Yes. See “Link a new prole to your Thermo Fisher Cloud
account“ on page 8.
Link a new profile to your Thermo Fisher Cloud account
From your new My Prole screen:
1. Touch Cloud.
2. Enter your Thermo Fisher Cloud account username and password.
If you need to create a Thermo Fisher Cloud account, go to thermosher.com/
cloud.
3. Touch Link account.
If the link is successful, you can proceed to use the instrument. If not successful,
repeat the above steps or touch Skip to use the instrument without connecting to
a cloud account.
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Link an existing profile to your Thermo Fisher Cloud account
1. Touch .
2. From your My Prole screen, touch Edit.
3. Touch Cloud.
4. Enter your Thermo Fisher Cloud account username and password.
If you need to create a Thermo Fisher Cloud account, go to thermosher.com/
cloud.
5. Touch Link account.
If the link is successful, you can proceed to use the instrument. If not successful,
repeat the above steps or touch Skip to use the instrument without connecting to
a cloud account.
If the unit is off
1. Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
2. Touch to sign in to use or set up a user prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. Images cannot be transferred between user and guest galleries.
3. Enter a Screen name.
4. Enter and conrm a four-digit pin.
5. Touch Create prole.
6. (Optional) If you want to link your instrument to your Thermo Fisher Cloud
account, touch Yes.
a. Enter your Thermo Fisher Cloud username and password.
b. Touch Link account.
7. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer and place sample in the center of the transilluminator
glass.
8. Touch to close drawer (do not physically push on the drawer). Instrument
adjusts and acquires a live-view sample image.
Start, sign on, and configure the instrument
Link an existing profile to your Thermo Fisher Cloud account
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If the unit is on and in active mode
1. If not already signed in, touch to sign in or set up a new user prole.
Note: For those not logged on as a user, acquired images are saved to a guest
gallery. Images cannot be transferred between user and guest galleries.
2. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer and place sample in the center of the turntable glass.
3. Touch to open drawer.
4. Insert sample into the middle of the viewing area.
5. Touch to close drawer.
Start, sign on, and configure the instrument
If the unit is on and in active mode
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Operate the iBright™imager
Choose imaging mode and start image capture
On the Welcome screen:
1. Use the drop-down menu to select the desired mode.
•Chemi Blots to image chemiluminescent substrates.
•Fluorescent Blots to image uorescent substrates.
•Nucleic Acid Gels to image DNA and RNA gels.
•Protein Gels to image visible, stained protein gels.
2. Touch to open drawer.
3. Place sample in the center of the viewing area.
4. Touch to close drawer.
Instrument automatically aligns, zooms, focuses, and acquires a live-view sample
image.
• To image in Chemi Blots mode, proceed to “Capture an image of the sample“ on
page 12.
• To image in Fluorescent Blots mode, proceed to “Image using Fluorescent Blots
(FL1000 model only)“ on page 13.
• To image in Nucleic Acid Gels mode, proceed to “Image using Nucleic Acid
Gel“ on page 15.
• To image in Protein Gels mode, proceed to “Image using Protein Gel“ on
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Chemi Blots
Mode to image chemiluminescent Western blots.
After you choose the imaging mode, capture an image of the sample.
If you are using the Fluorescent Blots mode (FL1000 model only), select one or more
dyes (see “Select a dye in the Fluorescent Blots mode“ on page 13).
IMPORTANT! To image a Coomassie or silver-stained gel using the Protein Gel mode,
place a white background under the sample.
1. Touch Smart Exposure.
The imager acquires a series of short exposures, then renders a preview image
and recommends an exposure time. This is not a real image.
2. (Optional) Adjust the exposure time using the following methods:
• Touch or .
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
3. Touch Enter.
The image preview updates in real-time.
4. Touch Capture to acquire an image.
The captured image is displayed and automatically saves to the gallery.
5. (Optional) Touch More options4Image adjust to optimize image or touch Trash
to delete the image.
Capture an image
of the sample
Operate the iBright™imager
Chemi Blots
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Fluorescent Blots (FL1000 model only)
Mode to image uorescent Western blots using a single channel or up to 4 channels
using excitation wavelengths of approximately 455-485 nm, 515-545 nm, 610-635 nm,
655-680 nm, and 745-765 nm (not available on CL1000).
1. Touch Choose a dye to select one dye or multiple dyes from the list. See “Select a
dye in the Fluorescent Blots mode“ on page 13 for selection details.
2. Touch Done.
3. Touch Smart Exposure. Alternatively, for manual exposure, touch More
options4Manual Exposure.
The imager will acquire a series of short exposures for each dye type selected. It
will then render preview images and a recommended exposure time for each
channel. These are not real images.
4. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
Note: To adjust or set the exposure time for a specic dye channel, see “Edit dye
channel exposure time“ on page 14.
5. Touch Enter.
The image preview will update in real-time.
6. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to steps 3-5 to adjust exposure conditions.
If you are capturing an image in the Fluorescent Blots mode (FL1000 model only),
select up to 4 dyes before imaging.
1. Touch a dye channel.
2. Select a dye from the list.
3. (Optional) Select a false color from the list and enter an exposure time.
4. Touch Done.
Repeat as many times as necessary, up to 4 dyes.
Image using
Fluorescent Blots
(FL1000 model
only)
Select a dye in the
Fluorescent Blots
mode
Operate the iBright™imager
Fluorescent Blots (FL1000 model only)
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1. Touch Edit channels.
2. Touch a dye buon.
3. Select a dye from the dye table.
User-created dyes are available as channels.
4. (Optional) Touch to toggle the dye overlay on the image on or o .
5. Touch Back to return to previous screen.
6. Touch Done.
After the initial Smart Exposure, you have the option to change exposure times for
individual dye channels.
1. Touch Smart Exposure. .
The imager will acquire a series of short exposures for each dye type selected. It
will then render preview images and a recommended exposure time for each
channel. These are not real images.
2. Touch Edit channels.
3. Touch a dye buon.
4. To adjust or set a manual exposure time, select a method:
• Touch + or – within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
input a user-specied time.
5. Touch Enter.
The image preview will update in real-time.
6. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
7. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to steps 3-5 to adjust exposure conditions.
Edit a dye in the
Fluorescent Blots
mode
Edit dye channel
exposure time
Operate the iBright™imager
Fluorescent Blots (FL1000 model only)
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Nucleic Acid Gels
Mode to image DNA and RNA gels stained with uorescent nucleic acid stains such
as Ethidium Bromide, SYBR™ Safe, SYBR™ Green (I or II), SYBR™ Gold and similar
products.
1. Touch Smart exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
2. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
3. Touch Enter.
The image preview will update in real-time.
4. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
5. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
Image using
Nucleic Acid Gel
Operate the iBright™imager
Nucleic Acid Gels
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Protein Gels
• Use this mode with the provided white screen to image protein gels stained with
colorimetric stains such as Coomassie and silver stains.
• Use this mode without the white screen to image proteins transferred to
membranes that have been stained with colorimetric stains such as Ponceau S and
Pierce™ Reversible Membrane Stain.
• Use this mode without the white screen to image protein gels stained with
SYPRO™ dyes.
IMPORTANT! For visual stained gels (e.g., Coomassie or silver-stained gels), place
white screen under the sample.
1. Touch Smart exposure. Alternatively, to set a manual exposure time, touch the
center of the dial.
The imager acquires a series of short exposures and then renders a preview
image and a recommended exposure time. This is not a real image.
2. To adjust or set a manual exposure time, select a method:
• Touch or within dial.
• Finger swipe in the segmented dial.
• Touch the dial center box to select a preset exposure time or touch Custom to
manually input a time.
3. Touch Enter.
The image preview will update in real-time.
4. Touch Capture to acquire image with the indicated exposure time.
Captured image appears on screen and automatically saves to the gallery.
5. If an acceptable image, touch Export, Gallery or Analyze. If an unacceptable
image, touch More options4Image adjust to optimize image or touch Trash to
remove image, then return to step 2 to adjust exposure conditions.
Image using
Protein Gel
Operate the iBright™imager
Protein Gels
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Options available in modes
Each mode oers More Options on certain workow screens to provide detailed
camera and image adjustment.
Table 3 Camera
Suboption Detail
Resolution/Sensitivity Changes binning setting to increase resolution/decrease
sensitivity or decrease resolution/increase sensitivity.
Zoom/Focus Changes zoom level to increase or decrease image area.
Optimizes focus for sharpness.
Sample rotation
Imager mechanically rotates sample ±10° depending on
orientation. Rotation is automatic, but also allows ability to
adjust in More Options4Camera Settings.
Table 4 Routines
Suboption Detail
Multi-exposure Series of 5 preset exposure times producing one image for
each time period.
Signal accumulation
Series of exposures where user defines the first capture
(time interval), the last capture (cumulative capture time)
and the total number of captures.
Signal accumulation
Note: Images acquired using Signal Accumulation are not
recommended for publication or analysis. This feature is
recommended for determining an optimal exposure time.
Table 5 Image adjust
Suboption Detail
Contrast
Grayscale differentiation between image features. Can use
Auto contrast, Gamma, White slider bar, or Black slider
bar.
Channels and Layers
Allow user to navigate between individual channels
associated with a common image file; pertains only to
Chemi and Fluorescent blot modes (e.g., Channels:
membrane, Alexa Fluor™488, Alexa Fluor™555,
Composite). Layers allow toggling on and off analysis tools.
Operate the iBright™imager
Options available in modes
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Suboption Detail
Zoom Increases or decreases digital zoom to select an area of the
image.
Invert Produces a negative or positive (white to black or black to
white) of the displayed image.
Saturation False-colors saturated white pixels (65,536) as red to
differentiate from non-saturated pixels.
Table 6 Other options
Suboption Detail
Region of Interest
Specify an area on the image to determine the optimal
exposure time for that region.focus the Smart Exposure
tool.
Manual exposure Manually select an exposure time for sample.
Smart exposure
Predicts optimal exposure for minimizing pixel saturation
and maximizing dynamic range for a specific sample.
Renders a preview of how the image will appear at the
recommended exposure time.
Band excision (Nucleic Acid
Gels mode)
Opens drawer and turns on LED light to allow for band
excision on nucleic acid gels.
Membrane overlay (Chemi
and Fluorescent Blot
modes)
Allows visible membrane overlay to see any visible
prestained markers, which aid in identifying molecular
weights for unknown samples.
Operate the iBright™imager
Options available in modes
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Suboption Detail
2UP view (Fluorescent Blot
mode)
After a Smart Exposure or Capture, images are displayed
by default in a 2UP view.
• Top image displays individual channels in grayscale.
Individual channels can be toggled on and off in the
grayscale image by touching Edit channels and on
the left of the appropriate dye. After a dye is selected,
that channel image is displayed in the top view and
becomes editable so the user can increase or
decrease the exposure time dial and see in real time
the effect on the image preview or captured image for
that specific channel.
• Bottom image displays the color composite with
channels displayed as different false colors overlaid
on a black background. Individual channels can be
toggled on and off in the composite image by touching
Edit channels and on the left of the appropriate
dye.
1UP view (Fluorescent Blot
mode)
After a Smart Exposure or Capture, images can also be
displayed in a 1UP view.
Image displays the grayscale and false-color composite
images by toggling Gray or Color. Individual channels can
be toggled on and off in the grayscale and false-color
images by touching Edit channels and on the left of the
appropriate dye.
Note: Functionality for toggling between individual
channels in grayscale view or toggling on and off individual
channels in the false-color composite view is identical to
the 2UP view.
Operate the iBright™imager
Options available in modes
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Image Gallery
Mode to retrieve, analyze, and manage images. Any image acquired on the imager is
automatically saved into a user's individual image gallery. For those not logged on as
a user (guest), acquired images are saved to a common gallery that houses all guest
image les.
On the Welcome screen or mode drop-down menu:
1. Touch Gallery or .
2. Toggle between Thumbnail view and List view to see stored images as
thumbnails or a table.
Click the column headers to sort the images.
3. Touch an image to select it. Touch again to deselect images. To choose all stored
images, touch Select all. Unselect using Deselect all.
4. To lter images, touch Filter options.
5. Touch Done.
View images
Operate the iBright™imager
Image Gallery
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Table of contents
