ThermoFisher Scientific Invitrogen iBright CL1000 User manual

For Research Use Only. Not for use in diagnostic procedures.
iBright™Imaging Systems
USER GUIDE
For use with the iBright™CL1000 Imaging System and the iBright™
FL1000 Imaging System
Catalog Numbers A32749 (CL1000) and A32752 (FL1000)
Publication Number MAN0015702
Revision A.0

Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL,
INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR
USE OF IT.
Revision history: Pub. No. MAN0015702
Revision Date Description
A.0 May 2017 New document. Describes installation, operation, and maintenance of the
iBright™Imaging Systems
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept
the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2017 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■Product information ....................................................... 6
Product description ............................................................. 6
Contents ....................................................................... 7
■Start, sign on, and configure the instrument ............................ 8
Create an instrument profile ..................................................... 8
Link a new profile to your Thermo Fisher Cloud account ............................. 8
Link an existing profile to your Thermo Fisher Cloud account ......................... 9
If the unit is off .................................................................. 9
If the unit is on and in active mode ................................................ 10
■Operate the iBright™imager ............................................ 11
Choose imaging mode and start image capture .................................... 11
Chemi Blots ................................................................... 12
Capture an image of the sample ............................................. 12
Fluorescent Blots (FL1000 model only) ........................................... 13
Image using Fluorescent Blots (FL1000 model only) ............................ 13
Select a dye in the Fluorescent Blots mode .................................... 13
Edit a dye in the Fluorescent Blots mode ...................................... 14
Edit dye channel exposure time .............................................. 14
Nucleic Acid Gels .............................................................. 15
Image using Nucleic Acid Gel ................................................ 15
Protein Gels ................................................................... 16
Image using Protein Gel .................................................... 16
Options available in modes ...................................................... 17
Image Gallery ................................................................. 20
View images .............................................................. 20
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■Export and analyze images .............................................. 21
Export images ................................................................. 21
Export new images ........................................................ 21
Export gallery images ...................................................... 21
Select an Export file type ................................................... 22
Analyze images ................................................................ 23
Analyze new images ....................................................... 23
Analyze gallery images ..................................................... 24
■Maintain the instrument ................................................. 26
Settings ....................................................................... 26
Manage accounts .......................................................... 27
Run instrument diagnostics ..................................................... 28
Clean the instrument ........................................................... 28
Replace the instrument fuses .................................................... 29
Materials required ......................................................... 29
Replace the fuses .......................................................... 29
Move the instrument ........................................................... 30
Prepare for shipping ....................................................... 30
■APPENDIX A Instrument specifications .............................. 31
Instrument dimensions and specifications ......................................... 31
Electrical requirements ......................................................... 31
Environmental requirements .................................................... 32
Network requirements .......................................................... 33
Instrument and computer connections ............................................ 34
■APPENDIX B Safety ..................................................... 36
Symbols on this instrument ..................................................... 36
Safety alerts on this instrument ............................................. 37
Location of safety labels on the instrument ................................... 38
Safety information for instruments not manufactured by Thermo Fisher Scientific ..... 38
Instrument safety .............................................................. 38
General .................................................................. 38
Physical injury ............................................................ 38
LED (Light-Emitting Diode) .................................................. 39
Electrical ................................................................. 39
Contents
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Cleaning and decontamination ............................................... 40
Instrument component and accessory disposal ................................ 40
Safety and electromagnetic compatibility (EMC) standards .......................... 41
Safety compliance ......................................................... 41
EMC ...................................................................... 41
Environmental design ...................................................... 42
Chemical safety ................................................................ 43
Biological hazard safety ......................................................... 44
■Documentation and support ............................................. 45
Related documentation ......................................................... 45
Customer and technical support ................................................. 45
Limited product warranty ....................................................... 46
Contents
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Product information
IMPORTANT! Before using this product, read and understand the information in the
“Safety” appendix in this document.
Product description
The iBright™ Imaging Systems allow users to image a variety of blots and gels. The
iBright™ CL1000 Imaging System provides for imaging of chemiluminescent Western
blots, DNA and RNA gels stained with uorescent nucleic acid stains, and visible
protein gels. The iBright™ FL1000 Imaging System adds the capability of imaging
uorescent Western blots using multiple dyes.
The iBright™ imagers use a simple design and intuitive workows to deliver high-
resolution images. The instrument can be run directly from the touchscreen to start
and create images, while also providing on-board software for image analysis. To
further analyze data, the instrument can be integrated with the iBright™ Image
Analysis Software available through your Thermo Fisher Cloud account.
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Parts of the instrument include:
1
2
3
1Touchscreen — Controls the instrument.
2USB port — For connection to an external network drive, jump drive, or other external
data storage device.
3Sample stage/turntable — Contains turntable for sample placement.
Contents
Table 1 Included imager system parts
Component Quantity
Power cord (region specific) 1
Quick reference card 1
White transilluminator screen 1
Reference target 1
Sample stage/turntable 1
Table 2 Available accessories
Component Part number
White transilluminator screen A33828
Sample stage/turntable A33829
High-Power USB Wi-Fi Module A26774
Product information
Contents
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Start, sign on, and configure the
instrument
Create an instrument profile
1. Touch to sign in to your instrument user account.
2. Touch Get started.
3. Enter a Screen name.
4. Enter and conrm a four-digit pin.
5. Touch Create prole.
6. (Optional) If you want to link your instrument to your Thermo Fisher Cloud
account, touch Yes. See “Link a new prole to your Thermo Fisher Cloud
account“ on page 8.
Link a new profile to your Thermo Fisher Cloud account
From your new My Prole screen:
1. Touch Cloud.
2. Enter your Thermo Fisher Cloud account username and password.
If you need to create a Thermo Fisher Cloud account, go to thermosher.com/
cloud.
3. Touch Link account.
If the link is successful, you can proceed to use the instrument. If not successful,
repeat the above steps or touch Skip to use the instrument without connecting to
a cloud account.
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Link an existing profile to your Thermo Fisher Cloud account
1. Touch .
2. From your My Prole screen, touch Edit.
3. Touch Cloud.
4. Enter your Thermo Fisher Cloud account username and password.
If you need to create a Thermo Fisher Cloud account, go to thermosher.com/
cloud.
5. Touch Link account.
If the link is successful, you can proceed to use the instrument. If not successful,
repeat the above steps or touch Skip to use the instrument without connecting to
a cloud account.
If the unit is off
1. Ensure the unit is plugged in and turn on using the switch on the back of
instrument.
2. Touch to sign in to use or set up a user prole.
Note: For those not logged on as a user (guest), acquired images are saved to a
guest gallery. Images cannot be transferred between user and guest galleries.
3. Enter a Screen name.
4. Enter and conrm a four-digit pin.
5. Touch Create prole.
6. (Optional) If you want to link your instrument to your Thermo Fisher Cloud
account, touch Yes.
a. Enter your Thermo Fisher Cloud username and password.
b. Touch Link account.
7. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer and place sample in the center of the transilluminator
glass.
8. Touch to close drawer (do not physically push on the drawer). Instrument
adjusts and acquires a live-view sample image.
Start, sign on, and configure the instrument
Link an existing profile to your Thermo Fisher Cloud account
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If the unit is on and in active mode
1. If not already signed in, touch to sign in or set up a new user prole.
Note: For those not logged on as a user, acquired images are saved to a guest
gallery. Images cannot be transferred between user and guest galleries.
2. From the Welcome screen, choose the appropriate Mode from the drop-down
menu, touch Open Drawer and place sample in the center of the turntable glass.
3. Touch to open drawer.
4. Insert sample into the middle of the viewing area.
5. Touch to close drawer.
Start, sign on, and configure the instrument
If the unit is on and in active mode
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