ThermoFisher Scientific Dionex DRS 600 User manual

Doc No. 1 November 2018
Thermo Scientific Dionex DRS 600, ERS 500e and ERS 500 Carbonate
Suppressor Installation Checklist
Thank you and congratulations on your recent purchase of a DIONEX suppressor product from
Thermo Fisher Scientific. To ensure a smooth installation experience, please review and
complete the following actions prior to installing the new suppressor:
Complete this Installation Checklist Sections I-II and check the box next to it to indicate
completion. If needed, complete Section III and check the box next to it to indicate completion.
If the instruction is unclear at any step, refer to the Installation Summary at the end of this
document.
I. Backpressure Check of Detector Cell and Backpressure Coils
Before putting the new suppressor to use, we recommend measuring the backpressure
to the suppressor, ensuring that the suppressor is not exposed to high backpressure
inadvertently. Care should be taken not to exceed 100 psi of backpressure to the
suppressor (150 psi for 2 mm anion versions). The backpressure to the suppressor comes
from any tubing, detector cell, backpressure coils, or any other components that are fluid-
connected to the eluent out port of the suppressor.
Step 1: Disconnect the column out/eluent in tubing from the old suppressor and
direct it to waste. Turn on the pump at the application flow rate for 10 minutes.
Record the system pressure 1 (P1) and turn off the pump.
□
P1, ________ psi
□
Step 2: Connect the column out/eluent in tubing to the detector cell inlet,remove
the backpressure coils after the detector cell if installed, and direct the detector
cell outlet tubing to waste. Turn on the pump at the application flow rate for 10
minutes. Record the system pressure 2 (P2) and turn off the pump.
□
P2,_________psi
□
Calculate the backpressure across the detector cell (BP1):
BP1= P2–P1= ________psi
The backpressure of the detector cell is typically ≤ 70 psi at a flow rate of 2
mL/min. If the backpressure exceeds this specification, see Detector Cell
Backflush Section
□
Step 3: Connect the recommended number of backpressure coil(s) (see table
below) to the detector cell outlet and direct the backpressure coil outlet to waste.
Turn on the pump at the application flow rate for 10 minutes. Record the system
pressure (P3) and turn off the pump.
□

Doc No. 2 November 2018
4 mm Chromatography
2 mm Chromatography
P/N: 045877
P/N: 045878
1 Coil = 1.5 –3.0 mL/min
1 Coil = 0.37 –0.75 mL/min
2 Coils = 0.5 –1.5 mL/min
2 Coils = 0.12 –0.37 mL/min
P3, ________ psi
□
Calculate the backpressure across the backpressure coil(s) (BP2):
BP2, = P3–P2= ________ psi
Pass: 30-40 psi for carbonate eluent or 10-40 psi for other eluents
If the back pressure exceeds the specification, see Backpressure Coil Adjustment
section
□
II. Hydration & Backpressure Check of Suppressor
Before putting the new suppressor to use, hydrate it properly first. Improper hydration
will result in irreversible damage to suppressor. This will void the warranty!
Step 4: Connect the column out tubing to the suppressor Eluent Inlet and direct
the suppressor Eluent Outlet to the Regen Inlet of the suppressor using ~ 6” of
green PEEK tubing (ID 0.030”) provided with the system ship kit. Direct the
suppressor Regen Outlet to waste (see Figure 1 below).
□
Turn on the pump and flow ≤10 mM eluent or DI water at 0.5 mL/min for 4mm
suppressor or 0.125 mL/min for 2mm suppressor for 20 minutes. The power to
the suppressor must be turned off during this step.
□
Figure 1: Suppressor Hydration Configuration

Doc No. 3 November 2018
Allow the suppressor to rest undisturbed for 20 minutes to fully hydrate the
resin, screens, and membranes.
□
Turn on the pump at the application flow rate for 10 minutes; record the system
pressure 4 (P4) and turn off the pump.
P4, _______ psi
□
Calculate the backpressure across the suppressor (BP3):
BP3= P4–P1= ________ psi Pass: ≤ 200 psi
If the backpressure exceeds this specification, see Suppressor Backflush with DI
Water section
□
Step 5: Connect the suppressor Eluent outlet tubing to the detector cell inlet.
Then connect the detector cell outlet tubing with backpressure coil(s) to the
Regen Inlet of the suppressor, as shown in Figure 2, for the Auto Suppression
Recycle Mode Configuration. For External Water Mode Configuration, refer to
the suppressor manual for detailed installation guide.
□
The suppressor is now ready to be installed and configured (see Configuration
of the New Suppressor below). Normal operation using the application eluent
strength, flow rate, and recommended current/voltage power settings can be
applied.
□
Figure 2: AutoSuppression Recycle Mode Configuration

Doc No. 4 November 2018
III. Troubleshooting Tips
Flow restriction or blockage from detector cell or backpressure coils should be eliminated
first to avoid irreversible damage to the suppressor.
Detector Cell Backflush:
Bypass the suppressor and connect the column outlet tubing to the detector
cell outlet port and direct the detector cell inlet tubing to waste.
□
Turn on the pump and flow eluent at the application flow rate for 10 minutes.
Turn off the pump. Re-measure the detector cell backpressure by following
the steps in Section I above.
□
Note: Repeat the backflush procedure if the backpressure remains high. If the
backpressure of the detector cell doesn’t come below 70 psi after multiple attempts,
replace the detector cell.
Backpressure Coil Adjustment:
If the pressure is greater than 40 psi for carbonate eluent or other eluents,
trim the backpressure coil and repeat the backpressure coil test in Section I.
□
If it is less than 30 psi for carbonate eluent or less than 10 psi other eluents,
add more tubing and repeat the backpressure coil test in Section I.
□
Note: Backpressure coils help to prevent gases generated during AutoSuppression from
out-gassing by compressing the bubbles. The bubbles can be occasionally trapped in the
detector cell causing noise. For example, carbonate eluent is suppressed to carbonic acid,
which is CO2gas in equilibrium with DI water, and CO2gas can come out of solution if
adequate pressure is not applied. Therefore, we highly recommend the addition of 30-40
psi for carbonate eluent or 10-40 psi for other eluents.
Suppressor Backflush with DI Water:
Turn off the pump and disconnect the column outlet and cell inlet tubing from
the suppressor eluent ports (if not already disconnected).
□
Connect the DI water tubing from the pump outlet directly to the suppressor
eluent out port and direct the eluent in tubing to waste. (Effectively plumbing
the eluent ports backwards to reverse flow through the eluent chamber).
□
Turn on the pump and flow DI water at 1.0 mL/min for 4 mm suppressor and
0.25 mL/min for 2 mm suppressor for 10 minutes. Turn off the pump. Re-
measure the suppressor backpressure by following the steps in Section II.
□
Note: Repeat the backflush procedure if the backpressure remains high. If the
backpressure of the suppressor doesn’t come below 200 psi after multiple attempts,
replace the suppressor or contact your Service Engineer or Technical Support team.

Doc No. 5 November 2018
Configuration of New Suppressor
When a new suppressor is plugged in, you’ll need to get into CM Configuration and open
up the Suppressor tab (ICS-6000) or Electrolytics tab (Integrion). Double click on the
Suppressor option and click OK to save the new configuration (see Figures 3 & 4 below). If
this is not done, CM will not recognize the new suppressor.
Figure 3: Suppressor Configuration in ICS-6000 DC Detector
Figure 4: Suppressor Configuration in Integrion

Doc No. 6 November 2018
Installation Summary