Timberline Instruments TL-105-D User manual

TL-105-D COLUMN HEATER
INSTRUCTION MANUAL
Timberline Instruments Inc.
1880 S. Flatiron Ct., Unit I
Boulder, Colorado 80301
Ph: [phone]
Fx: [phone]
[e-mail]
www.timberlineinstruments.com

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Timberline Model TL-105-D Column Heater
Instruction Manual
TABLE OF CONTENTS
TABLE OF CONTENTS ................................................................................................................................2
TL-105-D FEATURES...................................................................................................................................3
PRINCIPLE OF OPERATION.............................................................................................................................3
ADVANTAGES OF HPLC COLUMN TEMPERATURE CONTROL ...........................................................................3
ELECTRICAL DESCRIPTION ............................................................................................................................3
Heater and Control Circuit Descriptions.................................................................................................3
Thermal Fuse Protection ........................................................................................................................3
Electrical Fuse Protection.......................................................................................................................3
MOBILE PHASE HEAT EXCHANGER ................................................................................................................4
INJECTION OR SELECTION VALVE...................................................................................................................4
INSTALLATION.............................................................................................................................................5
CONNECTING THE TL-105-D.........................................................................................................................5
INSTALLING AN INJECTION OR SWITCHING VALVE............................................................................................5
CONNECTING THE COLUMN ...........................................................................................................................6
CONNECTING THE DRAIN...............................................................................................................................6
OPERATING INSTRUCTIONS .....................................................................................................................6
SETTING THE TEMPERATURE.........................................................................................................................6
APPENDIX.....................................................................................................................................................7
SPECIFICATIONS...........................................................................................................................................7
ORDERING INFORMATION ..............................................................................................................................7
WARRANTY ..................................................................................................................................................8
SHIPMENTS ..................................................................................................................................................8
DAMAGED SHIPMENTS ..................................................................................................................................8
FILING OF CLAIMS.........................................................................................................................................8
RETURNS .....................................................................................................................................................8
INDEX ..........................................................................................................................................................10

Timberline Instruments, Inc. - 3 - TL-105-D
TL-105-D FEATURES
PRINCIPLE OF OPERATION
Timberline’s Model TL-105-D Column Heater with integral mobile phase pre-heater is used in semi-
preparative HPLC applications where it is desirable to have independent temperature control of both the
column and mobile phase. The column heater provides temperature control with a stability of

0.1

C.
Typical accuracy of the RTD sensor and controller is within 1

C. The sophisticated microprocessor based
controller contains PID calibration capability and a high temperature shut off. The system is calibrated
before shipment and the PID constants are locked in memory.
ADVANTAGES OF HPLC COLUMN TEMPERATURE CONTROL
Controlling the temperature of HPLC columns has many advantages including
▪Retention time stability
▪Reduced detector noise and baseline drift
▪Simplified method transfer
Elevating the column temperature can improve separations.
▪Faster separation as retention decreases as temperature increases
▪Improved peak shape by reducing on-column silanol effects
▪Improved ion exchange efficiency
▪Lower backpressures by to reduction in mobile phase viscosity
▪Selectivity changes for better separations
ELECTRICAL DESCRIPTION
HEATER AND CONTROL CIRCUIT DESCRIPTIONS
The microprocessor base controller provides a 5-volt signal, actuating a solid-state relay which controls
power to the silicon heater. The controller sensor is a 100-ohm RTD probe inserted into a 0.14" hole in the
aluminum block. The RTD probe is placed very adjacent to the silicone heater to provide close temperature
control.
THERMAL FUSE PROTECTION
The heater is equipped with a secondary form of over temperature protection in a 12

C thermal fuse. If a
failure occurs in the electronic circuitry, the thermal fuse will protect the apparatus against an over
temperature condition. The thermal fuse must be manually reset when an over temperature condition opens
the heater circuit. Contact Timberline for instructions.
ELECTRICAL FUSE PROTECTION
The standard configuration for 120V operation is two 4A, 250V fuse. The configuration for 240V operation
is two 2A, 250V fuses.

Timberline Instruments, Inc. - 4 - TL-105-D
MOBILE PHASE HEAT EXCHANGER
The TL-105-D Column Heater comes equipped with a pre-column heat exchanger. This passive device
transfers heat from the floor of the heated zone to the inlet tubing. This action heats the incoming mobile
phase prior to entry into the HPLC column. Without preheating, there is a significant difference in
temperature between the incoming mobile phase and the column which greatly degrades column efficiency
and causes severe band broadening and peak splitting. This is due to radial temperature and viscosity
gradients in the column. When the mobile phase temperature is lower then the column temperature, the
fluid temperature in the heated column will be higher near the wall then it is in the center. This will result in
lower retention and viscosity near the tube wall causing peak broadening and splitting.
INJECTION OR SELECTION VALVE
The TL-105-D column heater has been designed to accommodate an injection or switching valve in the
heated compartment. This placement allows the entire flow path to be held at the same temperature. The
placement of the valve in the oven also allows for upstream preheating. In upstream preheating the heat
exchanger is placed between the pump and the injection valve eliminating extra column band broadening
from the volume of the heat exchanger.

Timberline Instruments, Inc. - 5 - TL-105-D
INSTALLATION
1. The TL-105-D will operate at 110, 220, or 240V (must be specified when ordering). Either 50 or 60-
hertz power is acceptable. The set instrument voltage is shown on the input receptacle.
2. The TL-105-D is furnished with a US style 110V AC line cord. If 220 or 240V is to be used, acquire
the proper power cord to conform to local protocol.
CONNECTING THE TL-105-D
1. Connect the mobile phase to be heated to the desired inlet of the TL-150-D, port.
2. Always use two wrenches when making and breaking connections to the bulkhead fittings.
DO NOT permit the bulkhead fittings to turn. Failure to do this will result in internal leaks.
3. The inlets and outlets may be connected in series depending on application.
INSTALLING AN INJECTION OR SWITCHING VALVE
The TL-105-D is designed to accommodate the mounting of an injection or switching valve. Since the valve
will reside in the same thermal environment as the heat exchanger and columns it is advised that the heat
exchanger be plumbed into the system before the valve. Placing the heat exchanger before the injection
valve will eliminate any extra column band broadening due to its volume.
1. Release the set screws on top of the oven and open the column compartment
2. Disconnect the heat exchanger from the bulkhead.
3. Remove the plate from the front panel of the TL-105-D by releasing the 4 hex head screws.
4. Remove the handle from the valve and insert the shaft through the larger hole on the front panel
plate. Secure it to the plate with the mounting screws. Replace the handle.
5. Position the plate on the front panel so that the valve goes through the hole in the column
compartment.
6. Before securing the plate, reconnect the heat exchanger fitting and connect the column outlet
tubing to the outlet bulkhead fitting on the inside of the plate.
7. Secure the plate to the front panel.
8. The injection valve vent/waste line should be routed out of the compartment through the hole in the
left side of the case.

Timberline Instruments, Inc. - 6 - TL-105-D
CONNECTING THE COLUMN
1. Connect the outlet of the manual or automated injection valve to the bulkhead labeled INLET. The
other side of this bulkhead is attached to the heat exchanger.
2. Release the set screws on top of the oven and open the column compartment.
3. Attach the outlet of the heat exchanger to the inlet of the HPLC column or guard column.
4. Attach the outlet of the HPLC column to the bulkhead side of the labeled OUTLET that is in the oven
faceplate.
5. Connect the bulkhead labeled OUTLET on the outside of the TL-105-D to the HPLC detector or post
column reagent module.
CONNECTING THE DRAIN
Any accidental leaks inthe columncompartmentwilldrainfrom thebackof the compartment tothebulkhead
on the right side of the oven near the lower back corner. Some corrosive or harsh organic liquids are
incompatible with the drain materials. Use caution to avoid leaks in the oven when using such liquids.
1. Connect a piece of tubing to the bulkhead using a nut and ferrule.
2. Direct the open end of the tubing to a waste reservoir.
OPERATING INSTRUCTIONS
SETTING THE TEMPERATURE
The set point can be adjusted with the two up/down arrow keys below the dual digital display. The
maximum set temperature for a standard TL-105-D is 100

C.
The nominal warm up time for the TL-105-D is 15-20 minutes. Generally, the unit should be left on
since the silicone heater is typically on a 10 - 30% duty cycle due to excellent insulation. Since the
heater current is controlled by an oversized solid-state relay there is no wear on the parts.

Timberline Instruments, Inc. - 7 - TL-105-D
APPENDIX
SPECIFICATIONS
Case Dimensions TL-105-D & TL-105-D-HT
8”W x 25.5”L x 5”H
Heated Cavity Dimensions TL-105-D
3.25"W x 23"L x 2.75"H
Inlet Pre-heater
0.0625” OD x 0.010” ID x 30” SS coil cast
in tin alloy
Heating Elements
Etched Foil/Silicon, 150W
Temperature Range TL-105-D
Application dependent
Tubing Material
316 SS or Hastelloy
Temperature Stability
+/- 0.1C
Operating Voltage
120 VAC or 240 VAC (Must be specified
by customer)
ORDERING INFORMATION
Description
Catalog Number
TL-105-D, 316 Stainless Steel, 120VAC
2115-D-1
TL-105-D, 316 Stainless Steel, 240VAC
2115-D-2
TL-105-D, Hastelloy, 120VAC
2115-D-H-1
TL-105-D, Hastelloy, 240VAC
2115D-H-2

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WARRANTY
Timberline Instruments, Inc. (Timberline) warrants its products and services to meet the written
performance and quality and to be free of defects in material and workmanship. They are not warranted,
nor does Timberline assume liability, if the buyer has misused the product in any manner, has failed to use
the product in accordance with industry standards and practices, or has failed to use the product in
accordance with instructions, if any, furnished by Timberline. Timberline’s sole responsibility and the buyer’s
exclusive remedy with respect to the purchase of any product proved to Timberline satisfaction to be
defective or nonconforming, is repair, replacement, or credit, in Timberline’s sole discretion. No other
warranty or representation is implied or expressed by Timberline for its products with respect to
merchantability, fitness for a particular purpose or any other matter. Timberline shall not under any
circumstances be liable for any incidental, consequential or compensatory damage arising from the use of,
or in conjunction with its products, even if Timberline has been advised of the possibility of such damages.
The maximum liability that can be assumed by Timberline for breach of warranty shall be the invoice price
of the product. All claims must be brought within one (1) year of shipment, regardless of their nature.
Components that are subject to normal wear and/or are scheduled for routine replacement within the
warranty period, and/or parts that are subjected to effects of corrosion or deterioration by chemical or other
action are excluded from the above warranty. Repair or replacement will not be made under warranty for
malfunction because of inadequate facilities, operating conditions or utilities.
Guarantees/Warranties on accessories and equipment included by Timberline from other manufacturers
are limited to the guarantees given on such equipment by the respective manufacturers.
SHIPMENTS
All shipments are made F.O.B. Boulder, CO. Instruments and major components will be packed and
shipped via surface, unless otherwise requested. Supplies and/or replacement parts are packed and
shipped via UPS, UPS Blue, air parcel post, or parcel post, unless otherwise requested.
DAMAGED SHIPMENTS
The Interstate Commerce Commission has held that carriers are as responsible for concealed damage as
for visible damage in transit. Unpack shipment promptly after receipt, as there may be concealed damage
even though no evidence of it is apparent. When concealed damage is discovered, cease further unpacking
of the unit involved and request immediate inspection by the local agent or carrier and secure a written
report of the findings to support a claim. This request must be made within 15 days of receipt. Otherwise,
the carrier will not honor the claim. Do not return damaged goods to the factory without first securing an
inspection report and contacting Timberline for a return authorization number.
FILING OF CLAIMS
After a damage inspection report has been secured, Timberline will cooperate in supplying replacements
and handling a claim that may be initiated by either party.
RETURNS
Timberline Instruments, Inc. tries to accommodate all requests for returns. Inspect shipment upon receipt
and report shortages, incorrect or damaged materials to us immediately. Report shipping damage to the
carrier. Damaged shipments must remain with the original packaging for freight company inspection.
Products not supplied in accordance with your orders or products that are defective at the time you receive
them are accepted for full credit. Products ordered in error are subject to a 15% restocking charge. Special
or custom orders cannot be returned unless defective. All returned merchandise must be in unused,
resalable condition, and must not consist of hazardous materials.
No returns will be accepted more then 90 days after shipment for any reason. Before 90 days, no returns
will be accepted without prior authorization. If it is necessary to return a product to us, please contact our
customer service department to obtain a return material authorization (RMA) and shipping instructions.

Timberline Instruments, Inc. - 9 - TL-105-D
When you call, be prepared to supply the information necessary for us to identify your order, including your
company name, address, purchase order number/invoice number, shipping date, product description and
catalog number. Write the RMA number on all shipping labels and correspondence about the shipment.
Returns without this number will be returned to you collect. Be careful to address the shipment to the street
address. Shipper will not deliver to our post office box numbers. We require that you prepay shipping costs;
COD's will not be accepted. Returns must be made through a traceable carrier.
Shipment of authorized returns should be made within 30 days of the issuance of RMA. If products are not
returned within the time limit, the RMA may expire. Timberline Instruments, Inc. reserves the right to refuse
any return or credit after a RMA has expired.

Timberline Instruments, Inc. - 10 - TL-105-D
INDEX
A
accuracy .....................................................................3
B
band broadening.........................................................4
C
column compartment will drain...................................6
corrosive or harsh organic liquids...............................6
D
drain............................................................................6
H
heat exchanger...........................................................4
I
injection valve ........................................................ 4, 5
P
peak broadening........................................................ 4
pre-column heat exchanger....................................... 4
S
set temperature.......................................................... 6
stability....................................................................... 3
switching valve....................................................... 4, 5
T
temperature,set.......................................................... 6
thermal fuse............................................................... 3
W
warm up time ............................................................. 6