Unico S1205 SERIES User manual

S1205 SERIES
SPECTROPHOTOMETER
USER’S MANUAL
V 3.0
United Products & Instruments, Inc.
182-E Ridge Road
Dayton, NJ 08810
732-274-1155 FAX [phone]
TABLE OF CONTENTS

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General information……………………………………………………………… 3
Safety………………………….........................................................……… 3
Electrical………………………………………………………………........ 3
Warning…………………………………………………………………….. 3
Performance………………………………………………………………… 4
Radio Interference………………………………………………………….. 4
Introduction…........................................................................……………… 4
Working Principle.......................................................................…………… 4
Unpacking Instructions.....................................................………………….. 5
Specifications….............................................................................................. 6
Installation.............................................................................…….................. 6
Operational Panel....................................................................……................ 8
Description of Keys....................................................................……............ 8
Operation Instruction............................................................……………................... 9
Prepare the spectrophotometer......................................................................... 9
BASIC MODE-%T/Abs Measure...............................................……………. 10
Quantitative Test.......................................................................……………… 12
Run test……………………………………………………………….. 12
Define/Establish Test Method……………………………………….. 14
By Known Factors……………………………………………. 15
By Standard………………………………………………….. 18
UTILITY……………………………………………………………………… 21
WL Calibration……………………………………………………….. 22
Dark Current………………………………………………………….. 22
EDIT CLOCK………………………………………………………… 22
SYSTEM BASELINE……………………………………..…………. 23
Turn on/off D2………………………………………………………… 24
Reset Defaults…………………………………………………………. 24
Memory Manager……………………………………………………… 24
WL Correction………………………………………………………… 25
PC LINK………………………………………………………………………. 25
Wavelength Calibration…………….……………………………….……………….. 26
Holmium Oxide Filter Method………………………………………………… 26
Didymium Filter Method……………………………………………………… 27
Absorbance Accuracy Checks………………………………………………… 28
Stary Light Check……………………………………………………………… 28
Trouble Shooting………………………………………………………….................. 29
Error Codes Definitions……………………………………………………………… 30
General information

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The apparatus described in this manual is designed to be used by properly trained personnel in a
suitable equipped laboratory. For the correct and safe use of this apparatus it is essential that
laboratory personnel follow generally accepted safe procedures in addition to the safety
precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the power
supply unit is a hazardous area and its cover should not be removed under any circumstances.
There are no serviceable components inside this power supply unit. For the 2100UV+, avoid
touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inflammable and samples
may be radioactive, toxic, or potentially infective. Care should be taken to follow the normal
laboratory procedures for handling chemicals and samples.
Safety
The safety statements in this manual comply with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its accessories. The S1205
should be operated by appropriate laboratory technicians.
Electrical
Before switching on the apparatus, make sure it is set to the voltage of the local power supply.
The power cord shall be inserted in a socket provided with a protective earth contact. The
protective action must not be negated by the use of an extension cord without a protective
conductor.
Warning
Any interruption of the protective conductor inside or outside the apparatus or disconnection of
the protective earth terminal is likely to make the apparatus dangerous. Intentional interruption
is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation. NEVER touch or handle the
power supply due to the high voltage.
The protection is likely to be impaired if, for example, the apparatus
•Shows visible damage
•Fails to perform the intended measurements
•Has been subjected to prolonged storage under unfavorable conditions.
•Has been subjected to severe transport stresses

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Performance
To ensure that the instrument is working within its specification, especially when making
measurements of an important nature, carry out performance checks with particular reference to
wavelength and absorbance accuracy. Performance checks are detailed in this manual.
Radio Interference
For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is
necessary that only shielded cables are used when connecting the instrument to computers and
accessories.
Introduction
The UNICO S1205 is single beam, general purpose instruments designed to meet the needs of
the Conventional Laboratory, They are ideal for various applications, such as: Clinical
Chemistry, Biochemistry, Petrochemistry, Environmental Protection, Food and Beverage Labs,
Water and Waste Water Labs and other fields of quality control and research.
Both S1205 features a digital display of photometric result, easy operation and wavelength range
of 325nm to 1000nm, the instrument is ideal for measurements in the visible wavelength region
of the electromagnetic spectrum.
Working Principle
The spectrophotometer consists of five parts: 1) Halogen lamp to supply the light; 2) A
Monochromator to isolate the wavelength of interest and eliminate the unwanted second order
radiation; 3) A sample compartment to accommodate the sample solution; 4) A detector to
receive the transmitted light and convert it to an electrical signal; and 5) A digital display to
indicate absorbance or transmittance. The block diagram below illustrates the relationship
between these parts.
Block diagram for the Spectrophotometer
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The grating

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disperses the light beam to produce the spectrum, a portion of which is focused on the exit slit of
the monochromator by a collimating mirror. From here the beam is passed to a sample
compartment through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment, the beam is
passed to the silicon photodiode detector and causes the detector to produce an electrical signal
that is displayed on the digital display.
The S1205 incorporate an RS-232C for printing and a USB port for connecting to a PC for using
the UNICO Application Software. There is a function select switch above the USB and RS-232 ports.
Set the switch at left for normal function (USB port for PC connection and RS-232 for printing). Set the
switch to the right for firmware update via RS-232 port (the USB port will not work at this setting).
Important Note:
The RS-232 port can also be used for firmware update. To update the firmware via RS-232 port
set the switch to the right (using null-modem RS232 cable).
Unpacking Instructions
Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition:
Unless otherwise specially ordered the spectrophotometer package should include the following
items.
Description: Quantity
Spectrophotometer...........……................. …........................ 1
Power Cord…………………………………......………….. 1
Cuvettes, Glass ..................................……............... …........ Set of 2
Dust Cover...................................…….......…. …................. 1
Manual................................................………....................... 1
Specifications for S1205
Model S1205
Wavelength Range 325-1000nm
Spectral Bandpass 5nm
Wavelength Accuracy + 2 nm
Wavelength Repeatability + 1nm
Stray Radiant Energy <0.3 @ 340 and 400nm
Photometric Range 0 to 125%T
-0.3 to 2.5 Abs
-9999 to 9999

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Photometric Accuracy + [email protected]
Data output RS232 and USB
Power Requirements 80-240Vac, 50-60 Hz
Dimensions 420W x 340D x 180H (mm)
Light Source Tungsten Halogen
Weight 8.5kg
Shipping Weight 19 lbs
Shipping dimension 20 x 16 x 11 inches
Installation:
1. After carefully unpacking the contents, check the materials with the packing list to ensure that
you have received everything in good condition.
2. Place the instrument in a suitable location away from direct sunlight. In order to have the
best performance from your instrument, keep it as far as possible from any strong magnetic or
electrical fields or any electrical device that may generate high-frequency fields. Set the unit up
in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air under and around the
instrument.
4. Turn on the instrument and allow it to warm up for 15 minutes before taking any readings.
Operational Panel
Description of Key Functions

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【CLEAR】Clear number or character when editing in Quantitative Mode;
【LOAD】Load saved data or saved curve ;
【PRINT】Print data;
【SAVE】Save data or curve;
【100%T】Set 0Abs and 100%T;
【EXIT】Return to previous screen;
【ENTER】Confirm the inputted data or selected item; Go to next setup or screen;
【∧】,【∨】Set Wavelength; Enter Standard solution value or Enter Known Factor
value; Browse the items in the menu
Operation Instruction:
Prepare the spectrophotometer
Turn on the spectrophotometer by pressing the Power Switch (IO). Press EXIT to skip 15
minutes warm up.
After 15 minutes warm up the main menu displays.
Note: a) If the saved wavelength has been out of order,`WL calibrating`will be
implemented and then go into Main Menu.
b) It is strongly recommended that taking dark current following the steps below
after warm-up:
1. Select UTILITY in main menu and then select Dark Current

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2. Dark Current will be taken shown and be saved shown after pressing EXIT
Important Notes: It is always a good practice to reset the dark current periodically.
It is especially important if the instrument is hanging on for a long time or the
environment is not stable.
BASIC MODE ━%T/Abs Measure
1. At main menu, scroll * to BASIC MODE and then press ENTER to Select basic T/Abs
mode. If the readouts are not 100.0%T and 0.000A, you may press 0Abs/100%T to blank.
BASIC MODE
2. Select the desired wavelength by pressing the appropriate key. At any time that the
wavelength is changed, blank will automatically be implemented.

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Select wavelength using【∧】,【∨】
Press Enter to set the new wavelength
Blank automatically
3. Make a blank reference solution by filling a clean cuvette with distilled or de-ionized
water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints
and droplets of liquid.
4. Fit the blank cuvette into the 4-cell holder and place the cuvette in the slot nearest you.
Push the rod so that the cuvette is in the light path. Close the lid.
5. Set 0.000A or 100%T by pressing 0A/100%T button.
6. Remove the blank cuvette if you are testing more than 3 samples. Set it aside in the case
that you may need to reset 0A/100%T later (i.e. change wavelength).
7. Rinse a second cuvette (or more) with a small amount of sample solution to be tested.
Fill the cuvette and wipe it.
8. Put the sample cuvette(s) in the sample compartment. Close the lid.
9. Read the % Transmittance and Absorbance from the LCD readout window. If you are
reading more than one cuvette, be sure to carefully move the cuvette holder to the next

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position by pulling on the sample holder rod until the holder “click” into place. Be sure
to make note of the readout (or print) for each sample. Remove the sample cuvette(s).
Operational Note:
1. If you are reading 3 or less samples, then place the reference cuvette in the position
nearest you, and the samples in the next available position. This will shorten the time to
read samples and minimize the sample handling (opening and closing the sample
compartment lid, etc.)
2. To save or print the readings please press PRINT/SAVE and follow the instruction on
line 4 of screen.
10. If you are to test the same sample at other wavelengths, repeat steps 2 to 9 for each
wavelength.
11. For each new sample you analyze, repeat steps 3 to 9.
Quantitative Test
Run Test using pre-defined Method
Test method (curve) must be defined and established before quantitative tests can be run.
This instrument has open platform for you to establish your own test methods (curves).
Such established method will be saved as defined test in “All Test List”. Refer to
“Define/Establish Test Method” section in this chapter.
Follow the procedures below to run quantitative test
1. At main menu scroll * to Quantitative and then press ENTER to Select Quantitative test
mode.
Fig 11
2. Select Run Test to run a pre-programmed and saved test. Place reference in the sample
holder and make sure it is in the optical path (working position). Scroll * to the left of the
desired test and then press ENTER to confirm.
Fig 12