Unico UV-2802 User manual

1
Contents
Safety ……….…………………………………………………………………. 2
General ………………………………………………………………………. 2
Electrical …………………………………………………………………….. 2
Warning ………………………………………………………………………….. 2
Performance ………………………………………………………………… 3
Radio Interference ………………………………………………………….. 3
Introduction ………………………………………………………………… 3
Working Principle ………………………………………………………….. 4
Unpacking Instructions ……………………………………………………. 4
Specifications ……………………………………………..……………………5
Installation ……………………………………………………………………….5
Operation ………………………………………………………..…………………6
Prepare the Spectrophotometer ……………………………………………6
Description of keys ………… ……………………………………… 6
Turn on spectrophotometer ……………………………………………….. 7
Basic operation………………………………………………………………. 8
Analyse Sample ………………………………………………………………12
Basic Mode …………………………………………………………….. 12
Quantitative ……………………………………………………….. 15
WL Scan ………………………………………………………………... 20
Kinetics …….………………………………………………………………… 25
DNA/Protein ………………………………………………………………28
Multi Wavelength ………………………………………………………….. 31
Setting and Calibration …………………………………………………. 33
Utility ……………………………………………………………………… 33
Defined Tests ……….…………………………………………………..43
Appendix A ………………………………………………………….. 46
Appendix B ……………………………………………………………47
Appendix C ……………………………………………………………54

2
Safety:
The safety statements in this manual comply with the requirements of the
HEALTH AND SAFETY AT WORK ACT, 1974.
Read the following before installing and using the instrument and its
accessories. The UNICOUV-2802 should be operated by appropriate
laboratory technicians.
General:
The apparatus described in this manual is designed to be used by properly
trained personnel in a suitable equipped laboratory. For the correct and safe
use of this apparatus it is essential that laboratory personnel follow generally
accepted safe procedures in addition to the safety precautions called for in this
manual.
The covers on this instrument may be removed for servicing. However, the
inside of the power supply unit is a hazardous area and its cover should not be
removed under any circumstances. There are no serviceable components
inside this power supply unit. For UNICOUV-2802, avoid touching the high
voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or
inflammable and samples may be radioactive, toxic, or potentially infective.
Care should be taken to follow the normal laboratory procedures for handling
chemicals and samples.
Electrical:
Before switching on the apparatus, make sure it is set to the voltage of the
local power supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth
contact. The protective action must not be negated by the use of an
extension cord without a protective conductor.
Warning:
Any interruption of the protective conductor inside or outside the apparatus or
disconnection of the protective earth terminal is likely to make the apparatus
dangerous. Intentional interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall
be made inoperative and be secured against any unintended operation.
NEVER touch or handle the power supply on UNICOUV-2802 due to the high
voltage.
The protection is likely to be impaired if, for example, the apparatus
ŸShows visible damage
ŸFails to perform the intended measurements
ŸHas been subjected to prolonged storage under unfavorable conditions
ŸHas been subjected to severe transport stresses

3
Performance:
To ensure that the instrument is working within its specification, especially
when making measurements of an important nature, carry out performance
checks with particular reference to wavelength and absorbance accuracy.
Performance checks are detailed in this manual.
Radio Interference:
For compliance with the EMC standards referred to in the EC Declaration of
Conformity, it is necessary that only shielded cables supplied by us are used
when connecting the instrument to computers and accessories.
Introduction:
The UNICOUV-2802 model spectrophotometer (Fig 1) is a single beam,
general purpose instrument designed to meet the needs of the Conventional
Laboratory, The UNICOUV-2802 model spectrophotometer is ideal for various
applications, such as: Chemistry, Biochemistry, Petrochemistry, Environmental
Protection, Food and Beverage Labs, Water and Waste Water Labs and other
fields of quality control and research.
The UNICOUV-2802 modelspectrophotometer incorporates a 320×240 dot
matrix LCD display for photometric results, easy operation and wavelength
range of 190nm to 1100nm. This instrument is ideal for measurements in the
visible and ultraviolet wavelength region of the electromagnetic spectrum.

4
Fig1
Working Principle:
The spectrophotometer consists of five parts: 1) Halogen or deuterium lamps
to supply the light; 2) A Monochromator to isolate the wavelength of interest
and eliminate the unwanted second order radiation; 3) A sample compartment
to accommodate the sample solution; 4) A detector to receive the transmitted
light and convert it to an electrical signal; and 5) A digital display to indicate
absorbance or transmittance. The block diagram (Fig 2) below illustrates the
relationship between these parts.
Block diagram for the Spectrophotometer
Light Mono-Sample Detector Display
Source chromator Compartment
Fig 2
In your spectrophotometer, light from the lamp is focused on the entrance slit
of the monochromator where the collimating mirror directs the beam onto the
grating. The grating disperses the light beam to produce the spectrum, a
portion of which is focused on the exit slit of the monochromator by a
collimating mirror. From here the beam is passed to a sample compartment
through one of the filters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Uponleaving the sample compartment,
the beam is passed to the silicon photodiode detector and causes the detector
to produce an electrical signal that is displayed on the digital display.
Unpacking Instructions:
Carefully unpack the contents and check the materials against the following
packing list to ensure that you have received everything in good condition.
100%T
0
A

5
Packing List
Description Quantity
ŸSpectrophotometer......................................................... 1
ŸMains Lead...................................................................... 1
ŸCuvettes........................................................................... Set of 4, glass
.................................................................... Set of 2, quartz
ŸDust Cover....................................................................... 1
ŸManual............................................................................. 1
Specifications:
ŸWavelength Range: 190-1100nm
ŸSpectral Bandpass: 4nm
ŸWavelength Accuracy: ±0.8nm
ŸWavelength Repeatability: ±0.05nm
ŸStray Radiant Energy: ＜0.15%@220nm&340nm
ŸPhotometric Range: 0-200%T,-0.3-.2.8A
ŸNoise:<0.001A @ 500nm 0A
ŸDrift: <0.002A/h @ 500nm
ŸPower Requirements: AC 110V/60Hz or 220V/50Hz
ŸDimensions: 550W×420L×270H
ŸLight Source: Tungsten Halogen/Deuterium
ŸWeight: 18kg
Installation:
1. After carefully unpacking the contents, check the materials with the
packing list (page4) to ensure that you have received everything in
good condition.
2. Place the instrument in a suitable location away from direct sunlight.
In order to have the best performance from your instrument, keep it as
far as possible from any strong magnetic or electrical fields or any
electrical device that may generate high-frequency fields. Set the unit
up in an area that is free of dust, corrosive gases and strong vibrations.
3. Remove any obstructions or materials that could hinder the flow of air
under and around the instrument.
4. Use the appropriate power cord and plug into a grounded outlet.
5. Turn on your UNICOUV-2802 model spectrophotometer. Allow it to
warm up for 15 minutes before taking any readings.We suggest you
then do the Calibrate System with the Search 656.1nm to set the
wavelength to the deuterium lamp emission line.
NOTE:
This symbol means Caution,Risk of Danger.Refer to this
Manual(see Appendix B–Lamp Replacement)

6
Operation:
Prepare the spectrophotometer
Fig 3 is the control panel. User can perform all operationsby pressing the
keys and all the results and operation information are displayed on the LCD.
Fig 3
ŸDescription of keys
【LOAD】 Load data or curve saved before;
【SAVE】 Save data or curve;
【SETλ】 Set wavelength;
【0Abs/100%T】 Blank or scan the base line;
【PRINT】 Print test results or screen
【START】 Start testing or scanning sample;
【ESC/STOP】 Exit to previous screenor cancel the operation;
【ENTER】 Confirm the inputted data or selected item; Go into next
setup or screen;
【F1】－【F4】 Function based on the information on the screen;
【0】－【9】 Input number or letter, consecutively press a numeric key
to select acharacter;
【+/-/.】 Input +,-or dot;
【CLEAR】 Clear all characterswhenyou areinputting or clear curve
displays on the screen;
【＜】,【＞】Change “x”scale; Search point after scan; 【＜】 clear a

7
character;
【∧】,【∨】Change “y”scale; Search peak after scan; Scroll items for
selecting; Change capital/small letter last typed in; Browse
the items for selection;
【CELL】Set cell position.
ŸTurn on spectrophotometer
Turn on spectrophotometer by pressing the Power Switch (IO)(see Fig1). The
instrument starts to initiate and the steps are as below:
1.The instrument will check memory first (Fig 4), please wait or press any
key to skip this step ,after positioning filter, auto-cell changer(if installed) and
D2/W lamps,the screen display as Fig 4A. 15 minutes pass or press【ESC】,
the screen display as Fig5,Select “No”to skip to main menu( Fig 7) and select
“Yes”(recommended) to calibrate system (Fig 6).The calibrating process
include “get dark current”,“searching 656.1nm”and “check energy”.After finish
the calibration system,go to main menu too (Fig 7).
2.If the data in memory has been lost, the instrument willdirectly
calibrate system without any choice for you.
3.If no auto-cell changer installed“cell #1”will disappear in Fig7
Fig 4
D2
W
Wait until EasyRTOS booted:
Check memory . . . . . . . .√
Init Printer . . . . . . . . . . .√
Init Comm Port . . . . . . . .√
Start Kernel . . . . . . . . . .√
Positioning . . . . . . . . . . √
Warm up 15 minutes . . .
Press ESC to skip ...
WL : 656.1nm 16:20:05
Fig 4A
United Products & Instruments Inc.
D2
W
Wait until EasyRTOS booted:
Check memory . . . . . . . .
WL : 656.1nm RAM Checked: 16kb

8
D2
W
Wait until EasyRTOS booted:
Check memory . . . . . . . .√
WL : 656.1nm 16:20:18
Init Printer . . . . . . . . . . .√
Init Comm Port . . . . . . . .√
Start Kernel . . . . . . . . . .√
Positioning . . . . . . . . . . √
System calibrating? No
Warm up 15 minutes. . . .√
Fig 5
D2
W
Wait until EasyRTOS booted:
Check memory . . . . . . . .√
United Products & Instruments Inc.
WL : 656.1nm 16:28:03
Init Printer . . . . . . . . . . .√
Init Comm Port . . . . . . . .√
Start Kernel . . . . . . . . . .√
Positioning . . . . . . . . . . √
Init AD Converter . . . . . .√
Search 656.1nm . . . . . .√
Check Energy . . . . . . √
Fig 6
WL : 656.1nm 16:31: 35
D2
W
Cell #1
1
2
38 7
4
5
6
Basic mode
Quantitative
WL scan
Kinetics
DNA/Protein
Multi WL
Utility
Defined test
United Products & Instruments Inc.
UNICO SPECTROPHOTOMETER
SPECTRO-QUEST
Fig 7
ŸBasic operation
Blank ²Push the blank cuvette into the lightpath.
²Press the key 【0Abs/100%T】for blanking
Note:1. If the reference solution is too thick, “Energy Low…”will appear
following the “Blanking…”on the screen (Fig 8).If “Energy too Low…” appears
following the “Blanking…”,the test will be paused and“Warning…”will appear
on the screen.(Fig 9).
2.If no automatic changer installed “cell #1”and “Max E”will disappear in
Fig8

9
WL : 656.1nm Energy Low. . .
Blanking D2
W
Cell #1
Max E
F1:Unit F2:Mode F3: FFactor F4: Standard
Fig 8
NOTE: 1.Blanking is automatic after a wavelength change
DO NOT OPEN SAMPLE COMPARTMENT LID DURING
BLANKING.
2.The dark current don’t be taken after power on,if you bypass the
calibrating system. It is recommended to take the dark current after
warm up.See page 38.
Warning…... D2
W
Cell #1
Max E
F1:Unit F2:Mode F3: FFactor F4: Standard
WL : 656.1nm Energy too Low. . .
Fig 9
Set wavelength (Example: set wavelength in “Basic mode”)
²Press【SETλ】(Fig 10).
WL : 656.1nm 12: 35: 27
0.001 Abs D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:FFactor F4:Standard
Fig 10

10
²Use numeric keypad to input wavelength (Fig 11).
WL : 656.1nm 12: 35: 27
0.001 Abs D2
W
Cell #1
Max E
Please input wl: 450
Fig 11
²Press 【ENTER】 to change the wavelength from 656.1nm to
450.0nm,and then blank;after blanking, the screen displays as Fig
12.
WL : 450.0nm 12: 35: 27
0.000 Abs D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard
Fig 12
Load or delete data or curve (Take the “WL scan”test For example)
Press【３】in Fig.7 go into “WL scan”.After【LOAD】being pressed,the
first file (ABC.wav)in memory will appear on the bottom line of
screen.Showed as Fig 13.Press 【∧】or【∨】 to browse the filesstroed
in memory. Then if:
1. The key.【ENTER】be pressed,the file selected will be loaded and
displays on the screen.Fig 14.
Note(1).The file selected must match “WL scan” test’s type.if not ，the
“file type error…”will appear on the right of top line.
(2).Different test has different file type.Refer to table 1 on page
12.
2. The key 【CLEAR】be pressed the file selected will be deleted by
selecting ”Yes”.

11
D2
W
Cell #1
WL : 680.0nm %T: 12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200.0
To:
680.0
Step:
1.0nm
XScale
YScale
F1:Setup F2:Mode F3:Search
0%T120.0
Fig 13
D2
W
Cell #1
WL : 680.0nm %T: 12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200.0
To:
680.0
Step:
1.0nm
XScale
YScale
F1:Setup F2:Mode F3:Search
0%T120.0
Fig 14
Table 1
Test File Type
Quantitative Curve ***.fit
Quantitative Test Result ***.qua
WL Scan ***.wav
Kinetics ***.kin
DNA/Protein ***.dna
Multi WL ***.mul
WL Validity ***. wlv
Accu. Validity ***.phv
Save data or curve (Example: Save curve in “WL scan”)
²Press the key 【SAVE】 in Fig14 to save curve.
²Name the curve by pressing the numeric keypad (Fig 15), press
the key【ENTER】 to comfirm.
. Note(1).Pressing numeric key continually to scroll characters and
pressing 【∧】,【∨】to alter capital letter to
miniscule.Table 2 shows all characters built in.
(2) If the name already exists in memory, the warning
“duplicated name, are you sure？” will appear .
“Yes”for overwrite and“No”for Exit.
(3) The length of filename is less than 4.

12
D2
W
Cell #1
WL : 680.0nm %T: 12: 35: 27
200.0 Wavelength (nm) 680.0
From:
200.0
To:
680.0
Step:
1.0nm
XScale
YScale
Please input File Name:2
0%T120.0
Fig 15
Table 2
key representing key representing key representing
00,+,-,*,/ 11,#,?,:,I 22,A,B,C,=
33,D,E,F,% 44,G,H,I,{55,J,K,L,}
66,M,N,O,~77,P,Q,R,S, 88,T,U,V,“
99,W,X,Y,Z +/-/. -,.,
Print test report (For example: Print the report in “Basic mode”,Fig16)
Press the key【PRINT】to print the report (curve or data you have loaded or
tested, Fig 17).
Fig 16
Fig 17
Before measurement
ŸMake a blank reference solution by filling a clean cuvette (or test tube) half
full with distilled or de-ionized water or other specifiedsolvent. Wipe the
cuvette with tissue to remove the fingerprints and droplets of liquid.
ŸFit the blank cuvette into the 4-cell linear changer and place the cuvette in
the slot nearest you. For the UNICOUV-2802, push the changer so that
WL : 546.0nm 12: 35: 27
0.221 Abs D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard

13
the cuvette is inthe light path (Push the rod in). Close the lid.
Analyze Sample
For different user requirements, we have provided different test methods.
Basic Mode
Push the blank cuvette into the light path. In main menu (Fig7),press【1】to
enter “Basic mode” test . After automatically blanking, it will display as Fig 18
(automatic changer installed) or Fig 19 ( automatic changer uninstalled) and
wait for the operator. 【ESC/STOP】to exit.
Note:.If no automatic changer installed“cell #1”and“Max E”will disappear
in Fig18
WL : 656.1nm 12: 35: 27
0.000 Abs D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard
Fig18
WL : 656.1nm 12: 35: 27
0.000 Abs D2
W
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard
Fig19
JTest
There are three modes(T%,Abs,conc/factor) for you toselect by pressing
【F2】to make choice.
WL : 656.1nm 12: 35: 27
0.104 Abs D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard
Fig 20

14
1. Abs mode
Push the blank cuvette into the light path.Press 【F2】 to select Abs
mode ,Press 【0Abs/100%T】for Blanking , and then Push the sample into
lightpath to take reading(Fig 20)
2. T% mode
The operation is the same as Abs test mode but pressing【F2】 to select
T% mode .
3. Conc/Factor mode
Press 【F1】 to select a concentration unit (Fig21). If no unit is
suitable for your test, please select the item “Other”,press enter and input a
new unit by pressing the numeric keypad (Fig 22).
WL : 656.1nm 12: 35: 27
D2
W
Cell #1
Max E
Please select unit: %
0.000 mg/ml
F factor 2.000
Fig 21
WL : 656.1nm 12: 35: 27
D2
W
Cell #1
Max E
Please input self defined unit: c
0.000 mg/ml
F factor 2.000
Fig 22
4. Push the blank cuvette into the light path and press【0Abs/100%T】for
Blanking.There are now two choicesfor you to take:
4.1 Press【F3】to input known F value, Fig 23. Then push the sample into
lightpath to take reading of concentration
4.2 Push sample of known concentration into the lightpath
Press【F4】to input known Conc value, Fig 24. Then push the sample
into lightpath to take reading of concentration.
Note:1.You can select wavelength at any time by pressing【SETλ】．
After your selection, instrument always blanksautomatically.
2.If F value is more than 9999,the “out of range”will display on
screen.

15
WL : 656.1nm 12: 35: 27
D2
W
Cell #1
Max E
Please input F factor: 4
0.000 mg/ml
Fig 23
WL : 656.1nm 12: 35: 27
0.000 mg/ml D2
W
Cell #1
Max E
F1:Unit F2:Mode F3:F Factor F4:Standard
F=4.000
Fig 24
JPrint Test Report
Press 【PRINT】to print test results (Fig 25).
Fig 25
Quantitative
Press【2】in Main Menu for “Quantitative”Test (Fig 26).Press【ESC/STOP】
to exit.
Note:.If no automatic changer installed“cell #1”will disappear in Fig26.
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Search
F1:Unit F2:Standard Curve
Quantitative Test
ID Abs Conc. (mg/L) WL(nm)
700.0
C=1.000*A^1 r=1.000
Scroll
Fig 26

16
JHow to operation
1. Press 【F1】to select unit of concentration(Fig 27).
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Please select unit: g/L
Quantitative Test
ID Abs Conc. (mg/L) WL(nm)
700.0
C=1.000*A^1 r=1.000
Search
Scroll
Fig 27
2Press 【SETλ】 to select correction methods and enter the
wavelength. There are three correction methods (single,
Isoabsorbance and 3 point, Fig 28) .
Note: Please refer to the Appendix C for the correction method.
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Quantitative Test
ID Abs Conc. (mg/L) WL(nm)
700.0
C=1.000*A^1 r=1.000
Correction method: Single WL
Search
Scroll
Fig 28
3.Press 【F2】in Fig 26 for more itemsto select .See Fig 29.
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Ｃａｌｉｂｒａｔｉｏｎ ｔａｂｌｅ
No Conc. (mg/L) Abs WL(nm)
700.0
C=1.000*A^1 r=1.000
F1:Method F2: Params F3:Standard F4: Curve
Fig 29
3.1Press 【F1】in Fig 29 to select fitting method. There are 4 methods for
you to choose:Linear fit, linear fit through zero,square fit and cubic fit.
3.2Press【F2】in Fig 29 to enter directlya known standard curve.Fig29A.

17
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Ｃａｌｉｂｒａｔｉｏｎ ｔａｂｌｅ
No Conc. (mg/L) Abs WL(nm)
700.0
C=1.000*A^1 r=1.000
Input K1=1.033
Fig 29A
The constants to be entered are depending on which fitting method selected.The
table below liststhe their relation:
Fitting Method Fitting Equation constants
linear fit through zero
C=K1×AK1, r*
Linear fit C=K0+K1×AK0,K1,r*
square fit C=K0+K1×A+K2×A2K0,K1,K2
cubic fit C=K0+K1×A+K2×A2+K3×A3K0,K1,K2,K3
* r :regression co-efficients, default=1
3.3Press 【F3】in Fig 29 to establish a standard curve by measuring a
group of standard samples.See Fig 30.
3.3.1 Enter standard concentrations of samples by pressing the
Numeric keypad followed by【ENTER】.Press 【∧】or【∨】 to
modify the inputted data Fig31.
Press【ESC/STOP】to finish inputting andto exitFig 32.
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Setup Standard Conc.
C=1.000*A^1 r=1.000
Edit the number . . .
No Conc. (mg/L) Abs
1 0.000
2 -more- WL(nm)
700.0
Fig 30
WL : 700.0nm Abs: 12: 35: 27
D2
W
Cell #1
Setup Standard Conc.
C=1.000*A^1 r=1.000
Input Standard Conc: 3
No Conc. (mg/L) Abs
1 2.000
2 3.000
3 -more-
WL(nm)
700.0
Fig 31

18
3.3.2 Push the blank cuvette into the light path, press
【0Abs/%100T】,the instrument will step to the wavelength and
blank. See Fig 32.
D2
W
Cell #1
F1:Method F2:Params F3:Standard F4:Curve
Calibration table
C=1.000*A^1 r=1.000
No Conc. (mg/L) Abs
1 2.000
2 3.000
3 4.000
4 5.000
5 6.000
WL : 700.0nm Abs: Blank ….…….
WL(nm)
700.0
Fig 32
3.3.3 Pull the first sample cuvette of known concentrationinto the
light path, Press the key【START】to get valuesof standard
curve one by one(Fig 33).
Note:If auto-cell changer is installed,the vary samples are
measured by pressing【CELL】following numbers(1-8)
and pressing【ENTER】to comfirm.
3.3.4 Press【F4】to draw the curve.You can geta different curve by
pressing【F1】 to select a different fitting method.
See Fig 34-Fig37.
For linear fits, “r”represent fitting coefficient oflinear
regression.r=1 is best fitting.usually “r ”is very close to 1.
Note:If there are few standard samples,it is not suitable for
selecting square fitting,especially cubic fitting,otherwise invalid
fitting result will be obtained.
D2
W
Cell #1
F1:Method F2:Params F3:Standard F4:Curve
Calibration table
C=1.000*A^1 r=1.000
No Conc. (mg/L) Abs
1 2.000 0.247
2 3.000 0.375
3 4.000 0.532
4 5.000 0.603
5 6.000 0.764
WL : 656.1nm Abs: 12: 35: 27
WL(nm)
700.0
Fig 33
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs: 12: 35: 27
-1.0 Abs 4.0
0Conc.76
Fig 34 linear through zero fit

19
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs: 12: 35: 27
-1.0 Abs 4.0
0Conc.76
Fig 35 square fit
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs: 12: 35: 27
-1.0 Abs 4.0
0Conc.91438
Fig 36 cubic fit
D2
W
Cell #1
Press (ESC) to return . . .
WL : 656.1nm Abs: 12: 35: 27
-1.0 Abs 4.0
0Conc.76
Fig 37 linear fit
3.3.5 Press 【SAVE】to save calibration if required
3.3.6Press【ESC/STOP】to exit
4.Quantitative Test
Before test,the standard curve must be obtained.There are three ways
for you toobtained it(a, b or c).
a) Standard curve built up and saved in the instrument.
In Fig 33 press【Load 】ａｎｄ ｔｈｅｎ ｐｒｅｓｓ【∧】or【∨】 to select the
file with type ***.fit. At last press【ENTER】ＴＯ ｃｏｍｆｉｒｍ．
b) Known standard curve, which is not saved in the instrument.
See 3.2.For Fig 29 enter a known standard curve directly.
c) Use the standard samplesfor the test. First the standard curve must
be established using the method shown in 3.3.
Note:All sample results must be taken inscreen Fig26.