VWR International 89508-420 User manual

User Manual
VWR®Collection Model 89508-420
Refrigerated 6.1 cu ft Incubator
Operating and Maintenance Manual 7003734 • Rev. 0

VWR Collection
MANUAL NUMBER 7003734
0 -- 10/7/13 Original ccs
REV ECR/ECN DATE DESCRIPTION By
Preface
Refrigerated Incubator i

VWR Collectionii Refrigerated Incubator
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. VWR International makes no representations or warranties with respect to this manual.
In no event shall VWR be held liable for any damages, direct or incidental, arising out of or related to the use of
this manual.
©2013 VWR International, LLC. All rights reserved.

VWR Collection Refrigerated Incubator iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. VWR International
has contracted with one or more recycling/disposal companies in each EU Member State European
Country, and this product should be disposed of or recycled through them. Further information on
VWR’s compliance with this directive, the recyclers in your country and information on VWR
products will be available at www.vwrsp.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Refrigerated Incubator ivVWR Collection
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Leg Leveler Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Door Reversal (if necessary) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Door Handle Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
General Functional Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2
Accessing Controller Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Changing Chamber Temp Control Setpoint . . . . . . . . . . . . . . . . . .5-3
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Defrosting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Suggested Defrost Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2
Replacing Temp Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-3
Replacing RTD Temperature Sensor . . . . . . . . . . . . . . . . . . . . . . . .6-3
Replacing FAN2, THM1, HTR1 . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Replacing SSR1, K1, K2 or T1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6

Refrigerated Incubator 1-1VWR Collection
Section 1 Introduction
Thank you for selecting VWR for your equipment needs.
The Refrigerated 6.1 cu ft Incubator offers precise temperature control
over the -10°C to 60°C range. Performance meets requirements for the
preservation of vaccines, biologicals, incubation of bacterial cultures, and
determination of bio-chemical oxygen demand of sewage. The extended
operating temperature range also includes temperatures associated with
drug stability projects, dairy product evaluation and entomological studies.
Features include:
• Spacious 6.1 cu ft chamber accommodates up to 114 standard BOD
bottles or similar containers
• Under the counter model with reversible door
• Two removable adjustable shelves (17.5”x 20.5”, 50 lbs. weight load.)
• Three molded door shelves (18.5”x 2.75”)
• Microprocessor-based temperature controller with temperature read-
out to 0.1°C
• Solid state relay for heater
• Over/undertemperature safety relay and alarm LED
• Overtemperature thermostat
• Cooling control modes:
1) High precision with cooling (100% mode)
2) High precision without cooling (00% mode)
3) Frost-free with variable cooling (50% mode)
• Compressor relay for energy conservation
• Compressor overload relay
• RTD temperature probe
• Protected setpoint mode to avoid accidental change
• Convenience outlet inside chamber, 2 amp (115V)

Refrigerated Incubator 2-1VWR Collection
Section 2 Safety Information
These instructions contain important operating and safety information.
The user must carefully read and understand these instructions before
using the refrigerated incubator. Your unit has been designed to optimize
function, reliability, safety and ease of use. It is the userʼs responsibility to
install the incubator in conformance with local electrical codes.
Warnings
1. Use a properly grounded electrical outlet of correct voltage and current
handling capacity. Check the nameplate on the back of the unit for
voltage and current rating. This equipment must be earth grounded for
safe operation.
2. Maximum ratings of accessories that may be plugged into convenience
outlet: load current 2A at 115VAC, leakage current 250 microamps.
3. Disconnect unit power cord from the power supply prior to
maintenance and servicing.
4. When operating at 100% cooling, the unit should be defrosted
periodically, especially during times of high humidity. The customer
will be responsible for any repair work done caused by evaporator ice
build up. See “Defrosting” section of this manual.
5. The aluminum evaporator and other portions of these units should not
be exposed to the corrosive effects of acidic or caustic materials.
Extreme care must be exercised if such materials are stored within to
prevent voiding the warranty. This incubator is NOT suitable for
flammable material storage.
6. This equipment must be used only as specified in these instructions. If
used in a manner other than as specified, the protection provided by
the equipment may be impaired.

Refrigerated Incubator 3-1VWR Collection
Section 3 Specifications
Power Requirements:
Catalog #89508-420 . . . . . . . . .120V, 9.5A, 60Hz
. . .including 2A for internal convenience outlet.
Dimensions:
Height: . . . . . . . . . . . . . . . . . . . .34.5 in (87.6cm)
Width: . . . . . . . . . . . . . . . . . . . . . . .24 in (61cm)
Depth: . . . . . . . . . . . . . . . . . . . . .24.5 in (62.2cm)
Volume: . . . . . . . . . . . . . . . . .6.1 cu ft (0.173m3)
Shelf Data: . . 2 chamber (50 lbs. weight capacity, 3 door)
Capacity: . . . . . . . . . . . . . . . . . .114 BOD bottles
Shipping Weight: . . . . . . . . . . . . . . .126 lb. (57 kg)
Operating Environment:
Indoor Use Only
Temperature Range: . . . . . . . . . . . . .10°C to 40°C
Humidity Range: . . . 0% to 90% RH Non-Condensing
Maximum Altitude: . . . . . . . . . . . .6600 ft (2 km)
Air Clearances: . . . . .Front and back, 3” minimum
Overvoltage Category II (IEC664)2
Pollution Degree 2 (IEC664)
Performance Characteristics:
Operating Range: . . . . . . . . . . . . . . .-10° to +60°C
Uniformity: . . . . . . . . . . . . . . . . . . . . . . . .±1.0°C1
Stability: . . . . . . . . . . . . . . . . . . . . . . . . . .±0.2°C1
Display Readability: . . . . . . . . . . . . . . . . . . .0.1°C
1Per ASTM method E 1292-94, cooling modes 00 and 100
2Voltage supply fluctuation not to exceed ±10% of rated voltage

Refrigerated Incubator 4-1VWR Collection
Section 4 Unpacking and Installation
The refrigerated incubator is shipped in a single carton. When unpacking
the unit, check each loose item against the list below. Should a shortage
exist, notify Technical Services.
If there is shipping damage, keep the entire shipment intact, retining the
carton and all packing material and file a claim with the final carrier.
Usually, the firm will send an investigator to ascertain liability.
Use the list below when unpacking to verify that the complete unit has
been received. Do not discard packing materials until all items are
accounted for.
The following items are included in the shipment:
Qty Item
1 Refrigerated incubator (89508-420 catalog number)
1 Leg leveler instructions
1Latching duct (used for door reversal)
1End cap for duct (in bag with J clamps)
3 J clamps (used for door reversal, in bag with end cap)
1 Plastic bag containing 4 feet
1 Door handle with 2 mounting screws
Unpacking

4-2 Refrigerated Incubator VWR Collection
Section 4
Unpacking and Installation
Leg Leveler
Installation
Use the adjustable feet to level the incubator.
1. Tip unit backwards so there is one foot of clearance on front of the
unit. Have someone assist in tilting the unit, to prevent the incubator
from falling while installing the leg levelers.
2. Screw front two leg levelers in until snug.
3. Tip the unit forward to screw in the back two leg levelers. Have
someone assist in tilting the unit, to prevent the incubator from falling
while installing the leg levelers. Leg leveler installation is complete.
4. If floor conditions do not allow the unit to sit level, adjust leg leveler(s)
by turning the required leveler counterclockwise to increase the height
and clockwise to reduce the height.
The Refrigerated Incubator should be installed on a level, stable floor.
Caution Do not place unit on its top, side, back, or front. If unit is
accidentally placed in any position other than right side up, the unit must
remain in upright position for at least 24 hours before connecting to
power. s
Locate the unit so that area in front of door and kick grille is clear. To
prevent heat build up within the unit, do not install it where it will be
exposed to strong sunlight, nor near radiators, furnaces, or other sources of
heat. Do not install the incubator where flammable or corrosive
atmospheres may exist. The Refrigerated Incubator is not to be used out-
of-doors or where excessive moisture or contamination could cause
degrading of mechanical or electrical components.
Installation
Figure 4-1. Leg Levelers

Refrigerated Incubator 4-3VWR Collection
Section 4
Unpacking and Installation
1. Remove all protective tape from doors and shelves.
2. Clean interior and exterior with warm soap and water. Shipping tape
residue can be removed using isopropyl alcohol.
3. Uncoil line cord from rear of unit. Connect the plug to a power outlet
that complies with the electrical requirements specified on the unit's
label and with proper safety ground connection.
4. Record serial number from unit on front cover of owner/operator
manual.
1. Disconnect unit from power source. On inside of door, directly behind
controller, open snapped conduit and remove controller cable.
2. Remove screw that secures cable clamp holding controller cable, then
replace screw.
3. Remove controller cable from anchors and ties that run along inside
chamber side and back walls.
4. Place 3 provided adhesive-backed mounting anchors at approximately
the same spacing (on opposite side) as existing anchors.
5. While supporting door with one hand, remove screw securing door to
upper hinge, then lift door off bottom hinge.
6. Remove the three screws securing each hinge to chassis.
7. Remove three plug screws covering holes on left side, then install them
in holes on right side.
8. Install hinges on left side using screws removed in Step 6. Position
hinge with door screw on bottom.
9. Position door on hinge, then insert screw previously removed in Step
5.
10. Attach supplied latching duct to inside on door, in line with controller
cable toward opposite side on door from other duct.
11. Route cable using mounting anchors, ties and latching duct.
12. Remove screw on left front of enclosure and install cable clamp with
removed screw.
13. Adjust size of loop in cable where door is hinged to ensure door can
fully open and close without interference.
Installation
(continued)
Door Reversal
(if necessary)

4-4 Refrigerated Incubator VWR Collection
1. Push the handle down over the top of door on left side until it
bottoms out. See Figure 4-2A.
2. While holding the handle in place, tighten the screws with a 1/4” nut
driver until they are tight against the top inner-side of the door. DO
NOT OVERTIGHTEN. SCREWS DO NOT GO INTO THE
DOOR.
Section 4
Unpacking and Installation
Door Handle
Installation
FRONT
OF
DOOR
HANDLE
(TOP OF DOOR)
(HANDLE)
SCREWS
Figure 4-2. Installing the Door Handle
A
B

Refrigerated Incubator 5-1VWR Collection
Section 5 Operation
Control descriptions are listed below.
Power ON/OFF Switch:
Located inside chamber area, is
used to turn power (I) ON or
(0) OFF to entire unit.
Note When power is first
applied, if display indicates S
instead of L, a switch setting on
the controller must be changed.
Refer to ‘Replacing Temp
Controller’ to change dipswitch setting as required. s
Keys located on front panel:
MENU - Used to access menus
SET - Used to display setting
s- INCREASE setting
t- DECREASE setting
Display (on front panel):
Displays chamber and set point
temperatures in °C and prompts
from menu.
Indicators (on front panel):
DELAY: Lights when delay start timer for compressor is timing. Will
begin timing when power is first applied or when cooling is first turned
off.
ALARM: Indicates that chamber temperature is not within limits and
the safety relay is opened.
COOL: Lights when cooling compressor is on.
HEAT: Lights when controller demands heat.
Figure 5-1. Power Switch
Figure 5-2. Front Panel

5-2 Refrigerated Incubator VWR Collection
Section 5
Operation
1. Turn power on to unit by placing the Power ON/OFF Switch to I
(ON) position. The Controller (mounted on front of door) will display
S , then, 88.8 (to test display), then actual chamber temperature.
2. After 3 seconds, the alarm LED on the controller should extinguish
and the safety relay should close, applying power to the heater, fan,
compressor relay and convenience outlet.
3. Press and hold MENU key, after 3-5 sec. the display will indicate CAL.
Release and press MENU again to display Pct, press SET, display
should indicate 100. Press and release MENU again to indicate Pro,
press SET, display should indicate 00, press and release MENU to
return to normal control mode. Press SET to display control setpoint,
display should read 25.0. If the control setpoint or any of the other
parameters aren't set correctly, use the up or down arrow keys while
pressing SET to change the setting.
4. The DELAY LED will be lighted for 7 minutes after power is first
applied, the COOL LED will be out during this delay period. When
the DELAY LED goes out, the COOL LED will light and the
compressor relay will close, starting the compressor.
5. Allow unit to stabilize for a minimum of 1 hour at 25°C, to ensure
that all systems are functioning.
Perform the procedure under General Functional Checks if incubator is
new or hasn't been operated for a long time.
Usually the only controller setting that is necessary to change is the
chamber temperature control setpoint. Other parameters of the controller
may be changed by accessing them through the MENU key, covered later.
General Functional
Checks
Operation
Controller

1. The chamber temperature is continuously displayed, unless a key is
pressed.
2. To display the setpoint temperature, press the SET key. The setpoint
temperature will remain on the display for 1 second after the SET key
is released.
3. To change the setpoint temperature, press and hold SET while pressing
the up or down arrow keys to change the setting. (Note that when
either up or down arrow keys are first pressed, the display will begin to
change slowly at first, then increase change rate after a few seconds.)
4. Select the desired chamber setpoint, then release all keys. The
controller display will revert back to actual chamber temperature after
a few seconds. Allow up to an hour for unit to stabilize at the new
setpoint temperature.
After the incubator temperature has stabilized, place samples into
incubator chamber. For best results, arrange samples evenly throughout the
chamber. Liquid samples should be covered to prevent evaporation and
eventual frost build-up on evaporator coils, particularly when operating
below ambient.
Note Frost build-up is normal when unit is being operated below 35°C,
with cooling set to 100% mode. s
The Refrigerated Incubator has an internal 2 amp convenience outlet
inside the chamber to operate devices such as: shakers, rotators,
photosynthesis lights, recording thermometers, etc.
The temperature controller has three menu selections that can be accessed
by pressing and holding the MENU key for 3 seconds. To access menu
parameters, press and hold the SET key. To change the parameter, use the
up or down arrow keys.
Note Once a menu parameter has been accessed, if no keys are pressed
within 8 to 10 seconds, the controller will automatically revert back to the
home display (measured control temperature). s
Refrigerated Incubator 5-3VWR Collection
Section 5
Operation
Changing Chamber Temp
Control Setpoint
Accessing Controller
Menus

CAL (Calibration) Menu - Used to change the temperature offset value to
correct for differences in chamber temperature and the displayed temper-
ature. Range -5° to 5°C. See Calibration Procedure.
Pct (Percent Cooling) Menu - Used to select one of three cooling modes:
100% Mode (-10°C to 60°C) - Compressor runs continuously which
provides high precision control (±0.2°C Stability) over entire temperature
range. Requires manual defrost.
Note All units are factory preset to 100%. s
50% Mode (15°C to 60°C) - Compressor runs approximately half the
time, which conserves power and also reduces the amount of frost
buildup on the evaporator. This method greatly reduces the frequency of
manual defrosting but provides less temperature stability (± 1.5°C).
00% Mode (35°C to 60°C) - Compressor is completely off. This mode is
ideal for incubating samples at temperatures 10°C or higher above ambi-
ent (35°C to 60°C). It provides the greatest power savings yet still main-
tain the advertised stability. No defrosting required.
Pro (Protection) Menu - Used to select the Protection mode of operation
that determines when the alarm is activated and if the control setpoint
can be changed. Range 00 to 02.
00 Mode- Normal mode that allows the user to change setpoint. Alarm
activates if chamber temperature (chamber) and setpoint temperature
(setpoint) are as follows:
chamber ≤ 15.0°C and chamber < (setpoint - 3) or
chamber ≥ 40.0°C and chamber > (setpoint +3).
Note All units are factory preset to 00. s
While ALARM is activated, the safety relay is opened, interrupting power
to the heater, fan, convenience outlet and compressor. When chamber
temperature returns to acceptable limits, the ALARM will automatically
terminate and power is reapplied. In order to allow changing of setpoint
and eliminate nuisance alarms, an alarm by-pass takes effect when the
setpoint is changed or when power is first applied. The alarm by-pass
works as follows: Present chamber temperature is stored as a nominal alarm
temperature (nominal). If chamber < setpoint then alarm temperature
(alarm) = nominal -3. If chamber > setpoint then alarm = nominal +3. If
chamber should go beyond alarm or < -13 or > 63 then the ALARM will
activate. This alarm by-pass is active until chamber reaches setpoint ±1°C.
5-4 Refrigerated Incubator VWR Collection
Section 5
Operation
Accessing Controller
Menus (continued)

01 Mode - Same as 00 Mode, except that the setpoint is locked and
can't be changed.
02 Mode - Setpoint is locked, plus, the ALARM will activate and the
safety relay will open as follows: chamber < (setpoint -3) or chamber
> (setpoint +3)
In order to use this mode of protection, modes 00 or 01 will have to be
used to reach the setpoint temperature. Once the chamber temperature
reaches the setpoint and is stable, protection mode 02 can be set.
This mode can only be used if the door is to remain shut and other
disturbances don't effect the chamber temperature.
As an added safety feature, the incubator has a thermostat in the heater
compartment that limits the upper chamber temperature to 65°C.
Note If the chamber temperature falls outside the setpoint by ±3°C, the
ALARM will activate and safety relay will interrupt power. s
Refrigerated Incubator 5-5VWR Collection
Section 5
Operation
Accessing Controller
Menus (continued)

Section 6 Maintenance
Warning To reduce risk of electric shock, disconnect unit from power
source before servicing. s
Before re-applying power, after maintenance is complete, check to ensure
that safety ground is intact and making a good connection.
Disconnect incubator from power source. Immediately clean all spilled
materials from the incubator and wipe dry. If necessary, moisten a cloth
with soap and water and clean inside and out. Do not use any harsh
chemical cleaners.
Periodic defrosting may be necessary. The time between defrosts depends
on operating temperature, ambient humidity and moisture from samples.
Time between defrosts may be from 30 days to years. Defrosting may be
necessary when operating at 50% mode as well, especially for control
temperatures less than 15.0°C.
Method I: For control setpoint down to 10°C, this method is the least
disruptive, if samples can withstand temperature stability of ±1.5°C for 24
hours. Samples may remain in chamber during this procedure.
1. (Refer to Accessing Controller Menus) Press and hold MENU key
until CAL is displayed, then release and press MENU again until Pct is
displayed.
2. Select 50 in the Pct menu then press MENU key twice to return
control mode.
3. Allow incubator to remain in this mode for about 24 hours or until
frost disappears.
4. Use the MENU key to re-establish the previous selection for Pct (i.e.
100).
Refrigerated Incubator 6-1VWR Collection
Cleaning
Defrosting
Suggested Defrost
Methods

Method II:
1. Remove any samples that may be damaged by temperatures up to
35°C.
2. Refer to Accessing Menu section of manual and set Pct parameter to
00.
3. Place Setpoint to 35.0°C.
4. Allow temperature to stabilize for 1 hour.
5. After 1 hour, remove power from unit. Open door and wipe up any
excess moisture on floor of chamber with paper towels or sponge.
6. Apply power and set desired operating parameters back into controller.
After chamber temperature stabilizes, place samples previously removed
back into unit.
If it becomes necessary to calibrate the temperature controller, perform the
following:
1. Place an accurate temperature-measuring device in the geometric
center of the chamber.
2. Set the controller to the desired operating temperature in 100%
cooling mode and allow 2 hours for stabilization.
3. Note the chamber temperature on the controller display and the
temperature at the geometric center.
4. Subtract the chamber display reading from the geometric center
reading.
5. Enter into the Calibration Mode as described in Accessing Menu.
6. Press and hold SET key, using the up or down keys, set the calibration
parameter to the value determined in Step 4.
7. Allow unit to stabilize for 1 hour and check that controller value and
geometric center value agree ±0.2°C.
Repeat calibration if necessary.
6-2 Refrigerated Incubator VWR Collection
Section 6
Maintenance
Suggested Defrost
Methods (continued)
Calibration

Refrigerated Incubator 6-3VWR Collection
Section 6
Maintenance
1. Disconnect incubator from power source.
2. Remove front panel bezel by removing four screws in top and bottom
of bezel.
3. Remove four screws that mount controller to mounting bracket.
4. Note wire colors and positions on TB1 and TB2, then remove all wires
from TB1 and TB2 and remove old controller.
5. Locate switch DS1 on lower left corner of new controller and set the
switches as follows:
Set DS1-1 (A) to off (up).
Set DS1-2 (B) to off (up).
6. Attach wires previously removed from old controller to new controller.
7. Mount new controller and bezel, then apply power.
8. Refer to Calibration section of this manual, to calibrate new controller.
1. Disconnect incubator from power source.
2. Refer to Replacing Temp. Controller, but only remove sensor wires
from TB2.
3. Open incubator door, remove Sensor on door bracket.
4. Remove old sensor, then place new sensor on bracket.
5. Connect new sensor wires to controller then mount controller and
bezel.
6. Apply power, then refer to Calibration section.
Replacing Temp
Controller
Replacing RTD
Temperature Sensor