3B SCIENTIFIC ME5 User manual

3B SCIENTIFIC®PHYSICS
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Monocular Microscope, ME5 1020249
Binocular Microscope, BE5 1020250
Trinocular Microscope, TE5 1020251
Instruction Manual
07/16 ALF
1. Description, technical data
The microscopes E5 allow two-dimensional viewing of objects (thin sections of plant or animal speci-
men) in 40x to 1000x magnification.
Besides the binocular eyepiece, the microscope model TE5 also offers the option of connecting a
camera for photographic and/or video documentation.
1 Eyepiece
2 Tube
3 Revolving nosepiece with objectives
4 Object stage with object guide
5 Condenser with iris diaphragm and
filter holder
6 Illumination
7 Mains switch
8 Head
9 Stand
10 x-y Cross-table adjustment
11 Retaining lever
12 Fine adjustment
13 Coarse and fine adjustment

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Art. No.
1020249
1020250
1020251
Description
Monocular Micro-
scope ME5
Binocular Micro-
scope BE5
Trinocular Micro-
scope TE5
Stand
Robust, all metal stand with arm permanently connected to the base. Fo-
cussing by means of separate knobs for coarse and fine adjustment located
on either side of the stand and operated by rack and pinion drive with ball
bearings and retaining lever, adjustable stopper for protecting the object
slides and objective.
Resolution of fine focussing adjustment: 0.002 mm
Tube
Monocular inclined 30°,
head rotation 360°
Monocular inclined 30°,
head rotation 360°
Monocular inclined 30°,
head rotation 360°
Eyepieces
Wide field plan eye-
piece PL 10x 18 mm
Wide field plan eyepiece PL 10x 18 mm
Objectives
Inverted objective revolver with 4 achromatic objectives 4x / 0.10, 10x / 0.25,
40x / 0.65, 100x / 1.25 (oil)
Enlargement
40x, 100x, 400x, 1000x
Object Stage
x-y mechanical stage, 132 mm x 140 mm, with object guide and coaxial
adjustment knobs perpendicular to the object stage, adjustment range 76
mm x 50 mm, accuracy 0.1 mm
Illumination
Adjustable LED lighting integrated in base, universal 100 to 240 V, 50/60 Hz
power supply
Condenser
Abbe condenser N.A.1.25 with iris diaphragm , filter holder and blue filter
Dimensions
350 mm x 213 mm x 366 mm approx.
Weight
8 kg approx.
2. Unpacking and assembly
The microscope is packed in a molded
styrofoam container.
Take the container out of the carton remove
the tape and carefully lift the top half off the
container. Be careful not to let the optical
items (objectives and eyepieces) drop down.
To avoid condensation on the optical com-
ponents, leave the microscope in the original
packing to allow it to adjust to room temper-
ature.
Using both hands (one around the pillar and
one around the base), lift the microscope
from the container and put it on a stable
desk.
The objectives will be found within individual
protective vials. Install the objectives into the
microscope nosepiece from the lowest mag-
nification to the highest, in a clockwise direc-
tion from the rear.
Insert the eyepiece into the tube and secure
it in place using the small hex key.
Secure the head with the large hex key.
3. Operation
3.1 General information
Set the microscope on a level table.
Place the object to be observed in the center
of the object plate.
Connect the mains cable to the net and turn
on the switch to get the object illuminated.
Make certain that the specimen is centered
over the opening in the stage.
Make the necessary eyepiece dioptre ad-
justments to suit your eyes (refer to 3.2).
Adjust the interpupillary distance so that one
circle of light can be seen (refer to 3.3).
Adjust the aperture of the iris diaphragm to
get the background brightness suitable for a
high contrast image.
Rotate the nosepiece until the objective with
the lowest magnification is pointed at the
specimen. There is a definite “click” when
each objective is lined up properly.
NOTE: It is best to begin with the lowest power
objective. This is important to reveal general
structural details with the largest field of view
first. Than you may increase the magnification
as needed to reveal small details.

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To determine the magnification at which you are
viewing a specimen, multiply the power of the
eyepiece by the power of the objective.
Adjust the coarse-focusing-knob which
moves the stage up until the specimen is fo-
cused. Be careful that the objective does not
make contact with the slide at any time. This
may cause damage to the objective and/or
crack your slide.
Adjust the fine-focusing-knob to get the im-
age more sharp and more clear.
Colour filters may be inserted into the filter
holder for definition of specimen parts.
Swing the filter holder out and insert colour
filters.
Be careful not to spill any liquids on the mi-
croscope.
Do not mishandle or impose unnecessary
force on the microscope.
Do not wipe the optics with your hands.
Do not attempt to service the microscope
yourself.
3.2 Dioptre adjustment (BE5 and TE5)
Look through the right-hand eyepiece with
your right eye and focus on the object.
Then look through the left-hand eyepiece
with your left eye. If the image is not well fo-
cussed, turn the dioptre adjustment ring (1)
until it is (see Fig. 1).
The dioptre adjustment ring (1) is marked with
dioptric values of ±5. The value to which the
scale marking (2) points corresponds to the
dioptric value for the user.
Set up the same value next time you use the
microscope as well.
Fig. 1 Dioptre adjustment
3.3 Setting of interpupillary distance (BE5
and TE5)
Look through the eyepieces with both eyes
and turn the tubes around the axle until you
can see only one image field.
Point (1) indicates the interpupillary distance of
the user on the scale (2) (see Fig. 2).
Set up the same interpupillary distance next
time you use the microscope as well.
Fig. 2 Adjustment of interpupillary distance
3.4 Adjustment of retaining attachment
You need to adjust the retaining attachment
(1) in order to fix the following problems: Dif-
ficulty turning the coarse focus adjustment
knob, slide slowly losing focus, sliding of ob-
ject stage (see Fig. 3).
Turning the knob for the retaining attachment in
the direction of the arrow secures the retaining
attachment. Turning in the opposite direction
loosens the focussing drive.
Fig. 3 Retaining attachment adjustment
3.5 Changing the lamp
Turn off the power switch and unplug the
mains plug.
Unscrew the base plate and remove it.
Unplug the connector to the LED unit by
pressing on the connector socket (1) and
pulling out the plug (2) (see Fig. 3).
Undo two screws (3), swap the faulty LED
unit for a new one and then screw the mi-
croscope back together.
Fig. 3 Changing the lamp

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Subject to technical amendments
© Copyright 2016 3B Scientific GmbH
4. Storage, cleaning, disposal
Keep the microscope in a clean, dry and
dust free place.
When not in use always cover the micro-
scope with the dust cover.
Do not expose it to temperatures below 5°C
and above 40°C and a max. relative humidi-
ty of over 80%.
Always unplug the mains plug before clean-
ing or maintenance.
Do not clean the unit with volatile solvents or
abrasive cleaners.
Do not disassemble objective or eyepieces
to attempt to clean them.
Use a soft linen cloth and some ethanol to
clean the microscope.
Use a soft lens tissue to clean the optics.
Die Verpackung ist bei den örtlichen Recyc-
lingstellen zu entsorgen.
Should you need to dis-
pose of the equipment
itself, never throw it away
in normal domestic waste.
If being used in private
households it can be dis-
posed of at the local public
waste disposal authority.
Comply with the applicable regulations for
the disposal of electrical equipment.