3D Histech Pannoramic MIDI II 2.0.5 User manual
PMII205-Rev1
Pannoramic MIDI II 2.0.5
User’s Guide
December 7, 2018
Rev. 1
Pannoramic MIDI II 2.0.5 User’s Guide
Contents
Disclaimer....................................................................................................................... 5
Declaration Of Conformity............................................................................................... 7
Character ormats and Symbols.......................................................................................8
Notes Regarding Operational Safety................................................................................9
Notes on Warranty........................................................................................................ 11
Terms and Abbreviations............................................................................................... 12
1 Product Description.................................................................................................... 13
1.1 Product Overview....................................................................................................................13
1.1.1 Intended U e..................................................................................................................................................13
1.1.2 Feature and Benefit .....................................................................................................................................13
1.1.3 Warning and Information Label ....................................................................................................................15
1.1.4 Ba e Unit........................................................................................................................................................16
1.2 Sy tem Overview......................................................................................................................17
1.2.1 Pannoramic MIDI II y tem.............................................................................................................................17
1.2.2 Hardware Option ..........................................................................................................................................18
1.2.3 Control Software............................................................................................................................................20
2 Installation................................................................................................................. 22
2.1 Control computer port ...........................................................................................................23
2.2 Connector and cable .............................................................................................................24
2.3 Coupling the Lumencor SOLA SM II unit with the canner......................................................24
2.4 Coupling the Lumencor Spectra 6 unit with the canner........................................................25
2.5 External power upply unit......................................................................................................25
2.6 Power the canner on/off........................................................................................................26
3 Preparing Slides and Trays........................................................................................... 27
3.1 Affixing Barcode Sticker to Slide ...........................................................................................29
3.2 In erting and Removing Slide .................................................................................................30
4 Software Description.................................................................................................. 33
4.1 Starting / Exiting Pannoramic MIDI II Software Application....................................................33
4.1.1 Starting the oftware......................................................................................................................................33
4.1.2 Exiting the oftware........................................................................................................................................34
4.2 Main window function ...........................................................................................................34
5 Scanning modes and settings......................................................................................44
5.1 Brightfield Scanning Mode.......................................................................................................44
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Pannoramic MIDI II 2.0.5 User’s Guide
5.1.1 Profile Setting Panel......................................................................................................................................45
5.1.2 Micro cope Control Panel..............................................................................................................................52
5.1.3 Quick micro cope function ...........................................................................................................................62
5.2 Fluore cence Micro cope Mode..............................................................................................64
5.2.1 Profile Setting panel......................................................................................................................................64
5.2.2 Micro cope Control Panel..............................................................................................................................74
5.2.3 Quick preview function .................................................................................................................................87
5.2.4 Bottom menu bar of the main window..........................................................................................................90
5.3 Planner mode...........................................................................................................................92
5.3.1 General function at the main menu.............................................................................................................93
5.3.2 General function at the tray menu...............................................................................................................95
5.3.3 General function at the lide menu..............................................................................................................95
5.3.4 Slide view with card........................................................................................................................................96
5.3.5 Table view mode.............................................................................................................................................98
5.3.6 Card collection view mode.............................................................................................................................99
5.4 Profile and Preview overview on the Planner.......................................................................100
5.5 Progre feature ...................................................................................................................101
5.6 Setting and Service...............................................................................................................102
5.6.1 Setting .........................................................................................................................................................102
5.6.2 Service .........................................................................................................................................................111
5.7 Ho ting...................................................................................................................................119
6 Using Profiles............................................................................................................ 121
7 Troubleshooting and Maintenance............................................................................123
7.1 Safety Information.................................................................................................................123
7.1.1 Cleaning the device......................................................................................................................................124
7.1.2 Cleaning the tray .........................................................................................................................................124
7.1.3 Preventive maintenance...............................................................................................................................124
7.2 Trouble hooting.....................................................................................................................125
7.2.1 Fluore cent Application (optional).............................................................................................................127
7.3 Maintenance..........................................................................................................................128
7.3.1 Opening the protective cover .....................................................................................................................128
7.3.2 Removing a lide...........................................................................................................................................129
7.3.3 In talling and Removing the Filter Module (Fluore cent Option)................................................................131
7.3.4 Tran porting Pannoramic MIDI II..................................................................................................................132
8 Technical Data........................................................................................................... 134
Appendix – Packaging instructions...............................................................................138
Preparation .................................................................................................................................138
Main part and their acce orie .................................................................................................138
Packaging.....................................................................................................................................140
Packing the MIDI II canner.........................................................................................................142
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Pannoramic MIDI II 2.0.5 User’s Guide
Packing the acce orie ...............................................................................................................142
Clo ing the package.....................................................................................................................142
Handling of the package..............................................................................................................143
Index........................................................................................................................... 144
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Disclaimer
Copyright © 2001-2018 3DHISTECH Ltd.
All right re erved.
Intellectual property related to the 3DHISTECH product are a follow :
EP 1,789,831; US 7,663,078; EP 2,024,772; JP 2009-512685; US 12/301,781; EP 1,644,767; JP 2007-
516451; US7,327,515; EP 1,994,398; JP 2008-557835; US 12/281,566; U0700210; US 6,246,785;
HU0900142; HU0700404; PCT/HU2007/000065; EP 08762668.5; US 12/663,537;
PCT/AT2006/000492; JP 2008-542552; EP 06817469; US 12/095,545; PCT/AT2006/000493; EP
06817470.5; US 12/095,596; HU0900741; US 61/264,732; US 11/826,752; P0401870;
PCT/IB2005/050344; US 11/662,976; HU0700409; US 12/042,050; US 77/636,144 and 007502032.
The above mentioned granted patent , granted utility model, regi tered trademark , pending
international and national patent application and the other corre ponding IP are the ole propertie
of the 3DHISTECH Ltd.
3DHISTECH Ltd. is not liable for damage of whatever nature (including, but not limited to, general
or specific damage, indirect damage, consequential damage or incidental damage, including the
results of the analysis of the digitized slides, for example, change of health status related to
erroneous diagnosis from the digitized slide(s)) that stems from or is associated with use of
Product, digitized slides, quality of staining, quality of stained slides, quality of used method of
staining. 3DHISTECH Ltd. assumes no responsibility for the functionality and fault-free condition of
your “application programs” (Workflows, VBA macros, Commander scripts).
CAUTION
Thi product ha not been approved or cleared a a medical device by the U.S. Food and Drug
Admini tration.
Do not u e thi device in clo e proximity to ource of trong electromagnetic radiation (e.g.
un hielded intentional RF ource ), a the e may interfere with the proper operation.
THE SO TWARE IS PROVIDED “AS IS” AND WITHOUT WARRANTY O ANY KIND, EXPRESSED,
IMPLIED OR OTHERWISE, INCLUDING WITHOUT LIMITATION, ANY WARRANTY O
MERCHANTABILITY OR ITNESS OR A PARTICULAR PURPOSE.
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Pannoramic MIDI II 2.0.5 User’s Guide
Declaration Of Conformity
3DHISTECH Ltd. declare that the product Pannoramic MIDI II lide canner i de igned and produced
with con ideration of pecified requirement according to the IEC 61326-2-6:2005 tandard, and
conform to the directive and other tandard li ted below:
IEC 61010-1:2001, “Safety Requirement for Electrical Equipment for Mea urement, Control, and
Laboratory U e - Part 1: General Requirement ”
IEC 61010-2-101:2002, “ Safety Requirement for Electrical Equipment for Mea urement, Control,
and Laboratory U e – Part 2-101: Particular requirement for in vitro diagno tic (IVD) medical
equipment”
UL 61010-1:2004 R10.08, “Safety Requirement for Electrical Equipment for Mea urement, Control,
and Laboratory U e - Part 1: General Requirement ”
Conformity with Directive IEC 61010-2-101 i marked by on the product labeling.
There i no further operator (u er) action required in ca e of re idual ri k .
Further information may be obtained from the manufacturer:
3DHISTECH Ltd.
3 Öv treet
1141 Budape t – HUNGARY
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Pannoramic MIDI II 2.0.5 User’s Guide
Character ormats and Symbols
Example Word or character that appear on the creen. The e include field name , creen
and window title , pu h-button and menu name , path or option .
Key on the keyboard. For example, function key ( uch a 11) or the Ctrl+M key
combination.
Example Cro -reference within thi document or to other document .
Warning! It might cau e irrever ible damage or harm to the u er of the product or the
product it elf if thi in truction i not followed.
Caution! It might cau e the product to not work properly if thi in truction i not followed
and alert to a potential danger ituation.
Note: Call attention to ome important feature .
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Pannoramic MIDI II 2.0.5 User’s Guide
Notes Regarding Operational Safety
The below ection of thi U er’ Guide contain information and warning of a kind that mu t be
followed by owner/operator per onnel.
Warning and advi ory ymbol which are u ed on the ba e unit of the Pannoramic MIDI II and in the
ection 1.1.3 Warning and Information Labels have the following meaning :
CAUTION
Di connect main plug before any kind of intervention into the device!
CAUTION
Cru h hazard!
The Pannoramic MIDI II, including any of it original acce ory component , hould not be operated in
any other way than de cribed in ection 1.1.1 Intended Use.
Any of the following note hould be trictly ob erved:
Warning! If operating the device other than pecified by the manufacturer, the level of
afety will decrea e.
Caution!
In the event of any non-conforming type of u e, including non-conforming u e
of a emblie or ingle part , no liability will be a umed by the Manufacturer.
Thi hall al o apply to ervice or repair work of any kind, which i performed by
other than authorized ervice per onnel. All claim for warranty and warranty
ervice will be null and void in any uch ca e.
Note: The afety level of the equipment after ervicing will be identical with level of
the original equipment.
Warning!
In ert the power cord plug only into a grounding outlet. The protective action
mu t not be rendered ineffective by exten ion cable without protective
conductor. The Pannoramic MIDI II ha a built-in protection which mu t not be
rendered ineffective by exten ion cable without protective (earth-ground)
conductor.
Warning! The operation of the in trument in explo ion-ri k environment i not allowed.
Warning!
If protective device are found to be ineffective, the Pannoramic MIDI II mu t be
hut down and precaution mu t be taken to prevent unintended u age. For
nece ary repair work or work to re tore proper operating condition, you
hould contact the 3DHISTECH Cu tomer Service.
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Pannoramic MIDI II 2.0.5 User’s Guide
Note:
Electric power to the Pannoramic MIDI II i upplied via an external main
power upply unit. It allow for operation at line voltage in the range of 100 V
to 240 V ±10%, 50/60 Hz, without any extra facilitie required for voltage
tran formation.
Warning! Check that your local line voltage i compatible with Pannoramic MIDI II
pecification before you turn power on.
Disconnect plug! Remember to unplug the power cord before you open the Pannoramic MIDI II
ba e unit for maintenance!
Warning! Only connect external device to the in trument which are approved to avoid
the ri k of electrical hock.
Warning!
The Pannoramic MIDI II doe not include any pecial facilitie to protect from
ample with an etching, potentially infectiou , toxic, radioactive or other
health-damaging effect. You are under obligation to comply with all currently
binding legi lation, notably, national accident prevention rule when handling
ample of thi kind.
Caution!
Dirt or du t may adver ely impact the operating capability of the Pannoramic
MIDI II. For thi rea on, mea ure mu t be taken to eliminate uch influence to
a maximum po ible degree. During period of non-u e, the Pannoramic MIDI II
mu t be protected with a du t cover. Check that power i turned off before you
put the cover on.
Caution! Do not po ition the equipment o that it i difficult to operate and to
di connect the device if nece ary.
Crush hazard!
There i cru h hazard in the movement area of the lide in ert during operation.
Plea e note that the digitizing proce ha to be interrupted and the in ert
moved via oftware to the output po ition before removing the in ert.
Warning! A defective Pannoramic MIDI II i not cla ified a dome tic wa te. It mu t be
properly di po ed in accordance with currently valid legal requirement .
Warning!
The Pannoramic MIDI II may not be operated by other than properly in tructed
per on . Operating per onnel mu t be fully aware of the potential danger
which a particular field of application involve . The Pannoramic MIDI II i a
preci ion in trument which may uffer a ignificant reduction in operational
function or even phy ical de truction following intervention performed in any
nonconforming manner.
Warning!
If a Lumencor SOLA SM II fluore cent illumination unit i u ed, the
corre ponding warning included in the manufacturer’ operating manual have
to be ob erved.
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Pannoramic MIDI II 2.0.5 User’s Guide
Notes on Warranty
3DHISTECH Ltd. – a the Product Manufacturer – warrant the Pannoramic MIDI II to be free from
fault in material and workman hip at the moment of in tallation. Defect mu t be notified
immediately on identification and maximum effort mu t be undertaken in order to minimize
damage. On receiving notice of a fault, the Product Manufacturer will be under obligation to remove
the fault, at hi own option, either by providing adequate repair ervice or by delivering a faultle
product for replacement. No warranty will be accepted in ca e of natural wear (notably, wear and
tear) or improper product handling.
The Product Manufacturer will refu e any liability in the event of damage re ulting from faulty
operation, negligence or unauthorized intervention into the product, notably, from the replacement
of product part or the u age of acce ory part from other manufacturer . Any uch ca e will void
the u er’ claim for warranty.
Except for action which are explicitly de cribed in thi U er’ Guide, you are prohibited from
performing any kind of maintenance or repair work at the Pannoramic MIDI II. Only authorized
3DHISTECH Service per onnel or pecifically authorized repre entative are allowed to handle repair
work. In ca e of malfunction contact 3DHISTECH Service Support fir t.
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Pannoramic MIDI II 2.0.5 User’s Guide
Terms and Abbreviations
CSV Comma Separated Value
OV Field of View
NA Numerical Aperture
Virtual Slide / Slide A digital image of a thin gla plate on which pecimen are mounted for
micro copic tudy:
a dynamic, interactive image that you can manage, ave, magnify, zoom, name,
evaluate, annotate, mark, comment, end to a colleague electronically for co-
operation or advice, and o on.
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Pannoramic MIDI II 2.0.5 User’s Guide 1 Product Description
1 Product Description
1.1 Product Overview
1.1.1 Intended Use
The Pannoramic MIDI II i de igned to u e tran mitted light and reflected light fluore cence to digitize
biological ample and pecimen (hi tological ection , typically). The target u er of the product can
work for example, in education, re earch, indu try or medical ector .
Warning!
After each canning make ure to in pect the digital lide for over-
expo ed area . In ca e the lide i canned ba ed on improperly et
parameter , it can highly affect the diagno tic re ult becau e of
information lo .
Warning!
Any modification to a hardware or oftware component of the PC will
highly affect the functionality of the canner program.
1.1.2 eatures and Benefits
The Pannoramic MIDI II offer world cla brightfield and fluore cent whole lide canning on up to 9
channel . An out tanding 0.12 µm/pixel (in Brightfield) and 0.16 µm/pixel (in Fluore cent) re olution
i achieved with the 40×/0.95 NA (equivalent to 80× magnification) Plan-Apochromat objective.
Key feature of the Pannoramic MIDI II canner:
•A ingle monochrome camera i u ed both for Brightfield and Fluore cent canning
•LED-ba ed RGB illumination unit
•Up to 9 freely electable color filter for fluore cence
•High-end fluore cent illumination device – Lumencor SPECTRA 6 Light Engine
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Pannoramic MIDI II 2.0.5 User’s Guide 1.1.2 Features and Benefits
•Multiband filter upport
•FL canning with a low noi e, 16-bit cientific CMOS pco.edge 4.2 camera
•New oftware-aimed flat-field correction compen ation method to create homogeneou lide
image
•12 lide automatic loading and canning
•Improved permanent autofocu
•Compact and robu t device
•Good quality and table manufacturing
•3MP camera for barcode reading and preview image
•Image harpening (for both Fluore cence and Brightfield) feature to improve the vi ual
appearance of digital lide
•Focu algorithm find the focu which i mo t appealing to the human eye
Pannoramic MIDI II i an optimal choice for cu tomer who want to can in brightfield and in
fluore cent mode where the quantity of canned lide will be regularly over 10 lide per day. It ha
the be t price/performance ratio with a reliable loading/ canning mechani m.
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Pannoramic MIDI II 2.0.5 User’s Guide 1.1. Warning and Information Labels
1.1.3 Warning and Information Labels
The following figure contain the label u ed on the Pannoramic MIDI II main unit, the external main
power upply unit, and the Lumencor SOLA SM II illumination unit.
Figure 1 – Warning and information labels
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Pannoramic MIDI II 2.0.5 User’s Guide 1.1.4 Base Unit
1.1.4 Base Unit
The following figure how the main part of the ba e unit.
Figure 2 – Main parts of base unit
1. Protective cover
2. Point Grey Gra hopper 3 or pco.edge 4.2 camera
luorescent option A
3. Lumencor SOLA SM II fiber-coupled illumination unit (can be u ed with Point Grey Gra hopper 3
camera only) with Lumencor illumination adapter and cable
luorescent option B
4. Lumencor SPECTRA 6 Light Engine fiber-coupled illumination unit
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Pannoramic MIDI II 2.0.5 User’s Guide 1.2 System Overview
1.2 System Overview
The tandard cope of delivery of the product include the following hardware and oftware
component .
1.2.1 Pannoramic MIDI II system
The following figure how the main part of the Pannoramic MIDI II y tem.
Figure 3 – Pannoramic MIDI II system overview
1. Pannoramic MIDI II ba e unit
2. 24 V external power upply unit for Pannoramic MIDI II
3. Lumencor SPECTRA 6 Light Engine illumination unit (option)
4. SOLA SM II fiber-coupled illumination unit (option)
5. External power upply unit for SOLA SM II
6. Slide tray
7. Control computer
8. Keyboard
9. Mou e
10. Monitor
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Pannoramic MIDI II 2.0.5 User’s Guide 1.2.2 Hardware Options
1.2.2 Hardware Options
Note:
The configuration of the control computer and monitor can vary
depending on the product order.
Cameras
Point Grey Grasshopper 3
1 CCD monochrome camera for high-quality
en itive fluore cent application .
pco.edge 4.2
Equipped with a high-quality CMOS image
en or for fluore cent application allowing fa t
canning and an out tanding low read out noi e.
High quantum efficiency, cooled with Peltier
element.
Camera adapters
0.63× adapter (for Point Grey
Gra hopper 3 only)
1× adapter
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Pannoramic MIDI II 2.0.5 User’s Guide 1.2.2 Hardware Options
Warning!
The in tallation of the camera in Pannoramic MIDI II mu t be carried
out by trained profe ional !
In any other ca e the warranty will be automatically voided. The
upplier or di tributor i not liable for any damage cau ed by an
in tallation carried out by an unauthorized per on.
Base Unit options
•Pannoramic MIDI II Ba ic Scan Option / Fa t Option – ba e unit equipped with Point Grey
Gra hopper 3 camera for Brightfield canning (no fluore cent acce orie included)
•Pannoramic MIDI II Ba ic Brightfield and Fluore cence Option – ba e unit completely
equipped with Point Grey Gra hopper 3 camera and Lumencor SOLA SM II (non-triggered)
or Lumencor SPECTRA 6 fluore cent illumination unit, with filter for fluore cence canning
•Pannoramic MIDI II Advanced Brightfield and Fluore cence Option – ba e unit completely
equipped with pco.edge 4.2 camera and Lumencor SPECTRA 6 fluore cence illumination
unit, trigger cable and filter for fluore cence canning
Note:
If Lumencor SPECTRA 6 unit i ordered, the cu tom light ource and
filter module can be variable. For more information, contact
3DHISTECH cu tomer ervice.
Objectives
•Plan-Apochromat 20×/NA 0.8
•Plan-Apochromat 40× Corr/NA 0.95
Warning!
Do not change correction collar etting on 40×/NA objective, if you
need it to be et, contact cu tomer ervice.
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Pannoramic MIDI II 2.0.5 User’s Guide 1.2.2 Hardware Options
luorescent Option for Pannoramic MIDI II
luorescent illumination unit Description
Lumencor SOLA SM II Fluore cent
illumination unit (can be u ed with
Point Grey Gra hopper 3 camera only)
Lumencor SPECTRA 6 Light Engine
illumination unit
1.2.3 Control Software
•Pannoramic Scanner control software
Optional:
•Fluore cence
•Extended focu
•Z- tack
•Barcode reader (licen ed feature)
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Table of contents
