abi PRISM 377 User manual

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Find the Thermo / Applied Biosystems Prism 377 at our website: Click HERE

ABI P
RISM
®
377 DNA Sequencer
96-Lane Upgrade
User’s Manual
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

© Copyright 2000, Applied Biosystems
For Research Use Only. Not for use in diagnostic procedures.
ABI PRISM and the ABI PRISM design, AmpliTaq, GeneAmp, and GeneScan are registered trademarks of PE Corporation or its
subsidiaries in the U.S. and certain other countries.
ABI,AFLP Plant Mapping,AmpF l STR,AmpF l STR Profiler,AmpF l STR Profiler Plus,ApliTaq Gold,Applied Biosystems, BigDye
Primer, BigDye Terminator, and True Allele, are trademarks of PE Corporation or its subsidiaries in the U.S. and certain other
countries.
All other trademarks are the sole property of their respective owners.
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Contents
i
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Upgrade Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Important Upgrade Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Kit Configurations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
2 Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Preparing Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Setting Run Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Loading Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
3 Software and Firmware . . . . . . . . . . . . . . . . . . . . . . 3-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
4 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
A Filter Set/Dye Combinations . . . . . . . . . . . . . . . . . .A-1
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

ii
B Two-Pitch, Eight-Channel Loader Suppliers. . . . . B-1
Supplier Information Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-1
C Part Numbers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
ABI P
RISM
377 DNA Sequencer Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-1
ABI P
RISM
DNA Fragment Analysis Kits and Reagents . . . . . . . . . . . . . . . C-3
ABI P
RISM
DNA Sequencing Kits and Reagents . . . . . . . . . . . . . . . . . . . . . C-8
User’s Manuals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-12
Part Number Updates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-12
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com