Agilent Technologies 1260 Infinity II User manual

Agilent 1260 Infinity II
GPC/SEC Column Thermostat
User Manual

Notices
Manual Part Number
D0029605 Rev. A
Edition 06/2023
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WARNING
CAUTION

GPC/SEC Column Thermostat User Manual 3
In this Guide….
This manual covers the Agilent 1260 Infinity II GPC/SEC Column Thermostat
(G7886A).
This manual is intended for laboratory technicians who are responsible for control
and day-to-day maintenance of the Column Thermostat. It is assumed that the
user of this manual has basic knowledge of how to use general purpose GPC/SEC
laboratory equipment and is familiar with standard laboratory terminology.
NOTE Read this manual including the safety instructions before using the Column
Thermostat.

GPC/SEC Column Thermostat User Manual 4
Contents
1Introduction 5
Safety Instructions 6
Safety Symbols 13
About the Column Thermostat 14
Identification of Parts 15
Options 17
Specifications 18
2Using the Column Thermostat 20
Setup Mains Power Connection 21
Setup Solvent Connections 22
Setup Waste Connection 25
Start the Column Thermostat 26
3Maintenance 27
General Cleaning 29
Check for Leaks 30
Replace Fuses 31
Service 32
4Parts for Maintenance 33
Columns Thermostat 34
5Appendix 35
Storage, Packaging and Recycling 36
Waste Electrical and Electronic Equipment (WEEE) Directive 37
Agilent Technologies on Internet 38

Introduction
GPC/SEC Column Thermostat User Manual 5
1Introduction
Safety Instructions 6
General Safety 7
Site Requirements 8
Power Considerations 9
System Operation and Maintenance 10
Solvent Information 11
Handling Leak and Waste 12
Applications: Quality Control 12
Safety Symbols 13
About the Column Thermostat 14
Identification of Parts 15
Instrument – Front 15
Instrument – Rear 16
Options 17
Cooling Option 17
Specifications 18
This chapter provides an overview of the most important functions and options of
the Column Thermostat.

Introduction
GPC/SEC Column Thermostat User Manual 6
Safety Instructions
In this manual, Warning / Caution messages are always indicated using the
following conventions:
WARNING
A warning alerts you to situations that could cause physical injury or death.
Do not proceed beyond a warning until you have fully understood and met
the indicated conditions.
CAUTION A caution alerts you to situations that could cause loss of data, or damage of
equipment.
Do not proceed beyond a caution until you have fully understood and met the
indicated conditions.

Introduction
GPC/SEC Column Thermostat User Manual 7
Adhere to quality control procedures and the following equipment guidelines when
using the system:
General Safety
WARNING
•
The system is intended to be used in a regulated laboratory environment with
trained operators following good laboratory practices.
•
Use this system ONLY for its intended use. Use of the system for any other
purpose may cause unsafe situations.
•
Do not use the system if there is any visible damage.
•
The system working environment should meet the specifications indicated in
this manual.
•
When installing the instrument, make sure that air circulation will not be
impeded.

Introduction
GPC/SEC Column Thermostat User Manual 8
Site Requirements
WARNING
•
Pay attention to the weight of the Column Thermostat. The instrument
weighs 17 kg (37.5 lbs) or 12.5 kg (28 lbs), without cooling option. Use
proper lifting techniques to avoid potential injuries. Ensure that you can
handle the weight of the load.
•
Consider the entire weight of the combined system including all the
components. The lab table on which this instrument is installed should be
sufficiently strong to support the total weight of the whole LC system. It
should be level, stable and have a depth of at least 600 mm. Otherwise, the
instrument could tip over or fall off the table.
•
Take measures to prevent the instrument from falling in the event of an
earthquake or other disaster. Strong vibrations could cause the instrument
to fall over, resulting in injury and damage.
•
All other equipment connected to this instrument must be approved to an
appropriate safety standard and have reinforced insulation from the mains.
•
Make sure the instrument is placed on a stable flat surface.
•
Make sure the instrument is placed in such a way that the mains power
connection can be reached easily to disconnect it from the mains power
supply by removing the mains power cable.
•
Use only cables provided with the instrument to prevent damage to the
system.
CAUTION
•
Make sure that the ventilation holes in the back panel and on the right-hand
side panel of the instrument are not obstructed. Leave at least 10 cm free
space on the right-hand side and at least 6 cm at the back of the instrument.
•
Do not install the instrument in places subject to excessive dust, direct
sunlight or shock and do not place it near a heat source, as this will influence
the cooling capabilities of the instrument.
•
Allow sufficient time to let the instrument reach ambient temperature before
switching it ON.

Introduction
GPC/SEC Column Thermostat User Manual 9
Bench Space
The module dimensions and weight (see Table “Physical Specifications”on page
18) allow you to place the module on almost any desk or laboratory bench. It needs
additional 10 cm (3.9 inches) space on right-hand side and approximately 6 cm
(2.4 inches) in the rear for air circulation and electric connections.
If the bench shall carry a complete LC system, make sure that the bench is
designed to bear the weight of all modules.
NOTE The instrument is heavy and thus it is advisable to locate the system on a sturdy
bench.
NOTE Allow additional bench space for a PC if the instrument is to be computer-
controlled.
Power Considerations
WARNING
•
To prevent electric shock in the event of an accident or electrical discharge,
the system must be connected to a suitable mains power supply with a
correctly installed protective earth conductor. Never use the system without
a properly connected protective earth conductor.
•
Read the operating instructions before connecting the system to the mains
power supply.
•Replace faulty insulation on power cords immediately.

Introduction
GPC/SEC Column Thermostat User Manual 10
System Operation and Maintenance
WARNING
•
Temperatures inside the Column Thermostat may be high. Touching the
panels inside the Column Thermostat may cause burns. Make sure the
actual temperature inside the Column Thermostat has decreased to a safe
value of, for example, 25°C before you open the front door to prevent injuries.
•
Use of biological materials, viral samples and needles may carry a significant
health risk. Always wear protective goggles and gloves when handling toxic
and biological infectious samples.
•
Removal of panels may expose users to lethal voltages.
•
Do not remove protective panels.
•To prevent electric shock, disconnect power before servicing the instrument.
CAUTION
•
To keep up the specified performance of the system, maintenance must be
carried out as indicated in this manual.
•
Service contracts and preventive maintenance services are available. Please
contact your local dealer or the nearest sales office for more information.

Introduction
GPC/SEC Column Thermostat User Manual 11
Solvent Information
WARNING
•
The solvents used may be flammable and toxic and may carry a significant
health risk. Therefore, the room in which the system is installed should be
well ventilated to prevent that solvent vapors cause a fire.
•
Do not allow solvents to accumulate in the system.
•
Perform periodic leak checks on fluid lines.
•
When you use or analyze toxic fluids you need to take all possible
precautions and treat all specimens as potentially toxic. Leaking solvent
connections can cause vapor accumulation in the Column Thermostat.
These vapors can be toxic. Make sure you do not breathe in vapors while
opening the door.
•
When you analyze biological fluids, you need to take all possible precautions
and treat all specimens as potentially infectious.
•
Always wear protective goggles and gloves when handling toxic and
biological infectious samples.
CAUTION When handling solvents always observe good laboratory practices. Know the
properties of the solvents used. This information can be found in Material Safety
Data Sheets (MSDS) supplied by the vendor. Avoid the use of the
following solvents
when using a steel containing flow path:
•
Solvents containing strong complexing agents like EDTA.
•
Strongly acidic mobile phases (pH <1).
•
Halogenated solvents or additives that form radicals and/or acids.
•
Solvents able to form peroxides like chromatographic grade ether and non-
stabilized THF, dioxane, and diisopropyl ether. In case use of these solvents is
unavoidable, they need to be filtered through dried aluminum oxide in order to
remove the peroxides.

Introduction
GPC/SEC Column Thermostat User Manual 12
Handling Leak and Waste
WARNING
Liquids associated with this instrument may be classified as carcinogenic,
biohazardous, flammable, or radioactive. Dispose of waste (samples, solvents,
device) in accordance with a regulated waste disposal program.
Follow a regulated, approved waste disposal program and never dispose of
solvents through the municipal sewage system.
Applications: Quality Control
NOTE It is recommended that you routinely run several quality control samples.
Quality control samples should represent low, average and high levels of a
compound. Make sure that quality control sample results are within an acceptable
range, and evaluate precision from day to day and run to run.
Data collected when quality control samples are out of range may not be valid. Do
not report this data until you are certain that system performance is acceptable.
Apart from use of quality control samples, we recommend that you use blanks. The
blanks will help you assess whether carry-over is within an acceptable range, and
they will help you in monitoring the integrity of your data.
It is also recommended that you use a proper internal standard.
If the system runs into an error state, the results for the processed sample should
not be trusted.

Introduction
GPC/SEC Column Thermostat User Manual 13
Safety Symbols
The following safety symbols are attached to the cover on the back of the
instrument. They are intended to alert you to the following safety information.
Table 1 Safety Symbols
Symbol
Description
The apparatus is marked with this symbol when the user
should refer to the instruction manual in order to protect risk of
harm to the operator and to protect the apparatus against
damage.
The hot surface sign calls attention to parts in the instrument
that must not be touched, as they may cause burns.
Confirms that a manufactured product complies with all
applicable European Community directives. The European
Declaration of Conformity is available at:
http://regulations.corporate.agilent.com/DoC/search.htm

Introduction
GPC/SEC Column Thermostat User Manual 14
About the Column Thermostat
Almost every parameter defining a chromatographic separation is temperature
dependent. For that reason, the Column Thermostat offers a stable and precise
temperature control. The Column Thermostat optimizes your GPC/SEC assay by
using forced air.
The Column Thermostat features:
quick heating
quick cooling (optional)
use of multiple columns (optional)
The Column Thermostat is compatible with most Agilent GPC/SEC instruments.
The instrument is controlled via a PC using LAN interface.
NOTE Procedures for use of control software are not included in this manual. Refer to the
documentation supplied with the software for more information.

Introduction
GPC/SEC Column Thermostat User Manual 15
Identification of Parts
WARNING
The temperature inside the Column Thermostat may be hot!
Make sure the temperature in the Column Thermostat has decreased to a
safe value before opening the front door.
Instrument – Front
Figure 1 Column Thermostat – front (multiple column setup)
1
Status LED
2
Fans; do not obstruct!
3
Columns
4
Door handle

Introduction
GPC/SEC Column Thermostat User Manual 16
Instrument – Rear
Figure 2 Column Thermostat – rear
1
Ethernet
2
OUT (serial) (not used)
3
IN (serial) (not used)
4
I/O connector
5
Ventilation holes; do not obstruct!
6
Fuse box, power connector, voltage selector,
ON/OFF switch: “I” indicates Power ON, “O” indicates Power OFF
WARNING
Any other instruments connected to this instrument must be approved to a
suitable safety standard and must include reinforced insulation from the
mains.

Introduction
GPC/SEC Column Thermostat User Manual 17
Options
The following options are available for the Column Thermostat:
Cooling Option
Temperature range from 5°C up to 90°C.
Without cooling option
Temperature range from ambient +10°C up to 90°C.

Introduction
GPC/SEC Column Thermostat User Manual 18
Specifications
Table 2 Physical Specifications
Type
Specification
Comments
Dimensions (height ×
width × depth)
600 x 170 x 345 mm
Weight
17 kg / 12.5 kg without cool option
(37.5 lbs / 28 lbs)
Operating altitude
up to 2000 m
Operating temperature
5 - 40°C
Operating humidity
20 – 80 % RH
Transport and storage
temperature
-29 - 60°C
Transport and storage
Humidity
Max. 85 % RH
Sound pressure level
LAeq <70 dB
Overvoltage category
II
For indoor use only
Pollution degree
2
Power requirements
100–120 V~ / 220–240 V~ ± 10 %, 50/60 Hz
Power consumption
550 VA maximum
Fuses
2 x T5AH, 250 V~, 1500 A
breaking capacity
IEC60127-2, UL recognized
ISM Classification
ISM Group 1 Class B
According to CISPR11

Introduction
GPC/SEC Column Thermostat User Manual 19
Table 3 Performance Specifications
Type
Specifications
Temperature range
With cool option:
5°C - 90°C, with 1°C increments 5°C - 75°C
Without cool option:
Ambient +10°C - 90°C, with 1°C increments
NOTE
Note that ambient temperature and humidity influence the
lower temperature limit of the Column Thermostat. When the
temperature and/or humidity go up, the lower temperature
limit will also go up. Typically, a ΔT of 18°C is feasible.
Temperature accuracy
Better than 0.1°C, measured at 30°C in the center of the Column Thermostat
Temperature stability
Better than 0.1°C, measured at 30°C in the center of the Column Thermostat
Temperature
reproducibility
Better than 0.1°C, measured at 30°C in the center of the Column Thermostat
Temperature change*
With cool option:
Typically, Up: 6°C/min from 40° to 60°C
Down: 2°C/min from 60°C to 40°C
Without cool option:
Up: 5°C/min from 40° to 60°C
Interfaces
Digital input and
output (TTL I/O)
2 programmable inputs, TTL 5 V
2 relay outputs, Vmax = 28 V, Imax = 0.25 A
Communication ports
Ethernet 10/100 Mb
* Tested for the 115/230 V AC ± 10 % voltage setting, usage ≤ 115 V AC will result in a lower
temperature change rate
Maximum length for IO cables is 3 m.
Table 4 Options
Type
Specifications
Cool / No-cool version
For details, see Table “Performance Specifications”on page 19.

Using the Column Thermostat
GPC/SEC Column Thermostat User Manual 20
2Using the Column Thermostat
Setup Mains Power Connection 21
Setup Solvent Connections 22
Setup Nut and Ferrule Connections 22
Setup Waste Connection 25
Start the Column Thermostat 26
This chapter explains the status of the Column Thermostat when the device is
initialized.