ALS SEC2020 User manual

Document Version: 2.5.0
Last Revision:July, 2019
SEC2020 SPECTROMETER SYSTEM
USER’S GUIDE
- DEVICE -

Device User’s Guide Contents
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Contents
1. SEC2020 Spectrometer system .........................................................................4
2. General Information.........................................................................................5
2-1 User Updates.................................................................................................. 5
2-2 Technical changes........................................................................................... 5
2-3 Damaged shipment ........................................................................................ 5
2-4 Product warranty ........................................................................................... 5
2-5 Service information........................................................................................ 6
3. System configuration.......................................................................................7
SEC2021 Spectrometer......................................................................................... 8
SEC2022 Deuterium Halogen light source .......................................................... 10
SEC2023 Cuvette holder ..................................................................................... 12
SEC2024 Platform............................................................................................... 13
AC adaptor and Power cable .............................................................................. 13
USB cable ........................................................................................................... 13
Collimator........................................................................................................... 14
Fiber collimator .................................................................................................. 14
External device connection trigger cable............................................................ 15
Trigger cable....................................................................................................... 15
Plastic cuvette .................................................................................................... 16
SMA905 adaptor for light source........................................................................ 16
SMA905 adaptor for light shielding .................................................................... 16
Hexagon wrench................................................................................................. 16
Software (USB memory)..................................................................................... 17
Quick manual ..................................................................................................... 17
Wavelength Calibration Data Sheet.................................................................... 17
Linearity Test Data Sheet.................................................................................... 17
Waterproof box.................................................................................................. 17
Warranty certificate ........................................................................................... 17
4. Instrument setting for absorbance/transmittance measurement .........18
Example: 1. Using cuvette .................................................................................. 18
Example: 2. Using thin film................................................................................. 19
Example: 3. Using probe..................................................................................... 20
5. Instrument setting for reflectance measurement.....................................22

Device User’s Guide Contents
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Application example using relectance probe...................................................... 22
6. Instrument setting for fluorescence measurement..................................24
Application example using high power LED ........................................................ 24
7. Instrument setting for light source measurement....................................25
Emission intensity measuremen for specific irradiation range measurement..... 25
8. Synchronization with other instruments.....................................................26
9. EU Declarationof Conformity .......................................................................30
10. Contact us .......................................................................................................31

Device User’s Guide 1. SEC2020 Spectrometer system
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1. SEC2020 Spectrometer system
The SEC2020 Spectrometer system is a compact spectrometer system developed by ALS
Co, .Ltd and provided by BAS Inc. One unit can measure a wide wavelength range from
ultraviolet to near infrared (UV/VIS/NIR) with high sensitivity, corresponding to optical
measurements such as spectrum, absorbance, transmittance, reflectance , fluorescence,
light source and concentration.
In addition, BAS, which also provides electrochemical measuring instruments, may also
offer know-how for spectroelectrochemical measurement, electrochemical
measurements combined with spectroscopy.
* Optional products may be prepared separately.

Device User’s Guide 2. General Information
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2. General Information
2-1 User Updates
In order to receive any updated information about product, and valuable information
related to current and other ALS products, please register your e-mail address at our
local distributors. We would like to know about your present status and interests
regarding electrochemical analysis.
2-2 Technical changes
We reserve the right to make technical changes to improve the instrument without
notice.
2-3 Damaged shipment
Breakage of any part of this instrument during shipping should be reported
immediately to the freight handler and ALS Customer service.
It is necessary to keep the original packing box and contents for inspection by the
freight forwarder. ALS will replace any new instrument damaged in shipping with an
identical product as expediently as possible after the claim filing date. Claims not filed
within 7 days after shipping may be invalid.
Do not return damaged goods to ALS directly. Please contact with your local
distributor informing them of its damaged status.
They will contact with our service department.
2-4 Product warranty
ALS Co., Ltd warrants equipment manufactured by the company to be free from
defects in material and workmanship for a period of 90 days from the date of
shipment. This assumes normal usage under commonly accepted operating
parameters. ALS agrees to either repair or replace, at its sole option and free of part
charges to the buyer, any parts of such instrumentation which, under proper and
normal conditions of use, prove to be defective within 90 days from date of shipment.
Electrochemical cells and working electrodes are warranted for 30 days.
ALS neither assumes nor authorizes any person to assume for it any other liability in

Device User’s Guide 2. General Information
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connection with the sale, installation, service, or use of its instrumentation.
All products manufactured by ALS are tested and inspected prior to shipment. Upon
prompt notification by the buyer, ALS will correct any defects in warranted
equipment by its manufacture either by return of the item to our factory, or
shipment of a repaired or replacement part. ALS will not be obliged, however, to
replace or repair any piece of equipment which has been abused, improperly
installed, altered, damaged or repaired by others. Defects in equipment do not
include decomposition, wear, or damage by chemical action or corrosion.
This instrument is manufactured, either wholly or in part, for research purposes only.
Use in medical diagnosis is not intended, implied or recommended by the
manufacturer. Use for this purpose and accountability for the same rests entirely with
the user.
Limited obligations covered by this warranty include:
In the case of instruments not of ALS manufacture, the original manufacturer’s
warranty applies.
Shipping charges under warranty are covered only in one direction. The buyer is
responsible for shipping charges to the factory, if return of the part is required.
Expendable items including disposable items such as working electrode, reference
electrodes, source lights, panel lights, fuses, etc. are excluded from the warranty
2-5 Service information
ALS provides a skilled service staff to solve your equipment oriented problems. For
further details, please contact us by e-mail ([e-mail]).
Following discussion of your specific difficulties, an appropriate course of action will
be described and the problem resolved accordingly.
Please contact with local distributor and describe to them the problem you are
having in full detail.
They will obtain a RETURN MERCHANDISE AUTHORIZATION NUMBER (RMA#). The
RMA# identifies you as the sender. All correspondence and shipments should be sent
to ALS.

Device User’s Guide 3. System Configuration
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3. System configuration
SEC 2020 spectrometer system consists of four basic accessories. It consists of SEC 2021
spectrometer, SEC 2022 deuterium halogen light source, SEC 2023 cuvette holder, and
SEC 2024 platform.
12
3
4
5
67
10
1314
8
12
15
916
17
18
11
Figure 3-1: Configuration and List of accessories
Spectrometerer
Cuvette holder
Light source
Platform
De sc ription Qty
1 SEC20 21 Spectrome te r 1
2
SEC20 22 Deu te rium halogen light source
1
3 SEC20 23 Cu vette h older 1
4 SEC20 24 Platform 1
5 AC adapto r 1
6 Power c able 1
7 USB cable 1
8 Collimator 2
9 Fibe r collimator 1
10 Platform sc rew 7
11
External device conn ection trigge r cable
1
12 Trigger c able 1
13 Plastic c uvette 1
14 SMA905 adaptor for light sourc e 3
15 SMA905 adaptor for light shielding 2
16 Hexagon wrenc h0.8 9mm 1
17 Hexagon wrenc h1.5 0mm 1
18 Software （USB me mory） 1
(19) Quick manual 1
(20) Wavelen gth Calibration Data Sh eet 1
(21) Lin earity Test Data She et 1
(22) Waterproof box 1
(23) Warran ty certificate 1

Device User’s Guide 3. System Configuration
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SEC2021 Spectrometer
SEC2020 Spectrometer system is equipped with SEC 2021 spectrometer as standard
content. With highly functional grating and optical design, it is possible to measure a wide
wavelength range from ultraviolet to near infrared (UV / VIS / NIR) with high sensitivity
with one unit.
Spectrometer specification
Description
SEC2021-025-DUVN
Detector
2048 element linear silicon CCD array
Wavelength range
200 –1025 nm
Grating
Blaze wavelength (300 nm)
Slit
25 μm
Wavelength resolution
1.3 nm
Fiber connector
SMA905 Core diameter: 600 μm NA = 0.22
Interface
USB 2.0
Operating system
Windows TM 7/8.1/10, 32/64 bit
Size (W x D x H)
86 x 110 x 32 mm
Figure 3-2: SEC2021 Spectrometer

Device User’s Guide 3. System Configuration
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I/O Port Pin
The Pin number of the I/O port for the spectrometer controlling is showing below:
Pin No.
Signal
Definition
Function
1
Power
5V Output
When connecting to the PC's USB port, this pin is connected to
VBUS. Up to 100 mA current
2
No connection
3
No connection
4
Output
Shutter
Shutter ON/OFF
5
Output
D2_ON
D2 light source ON/OFF
6
Output
HAL_ON
Halogen light source ON/OFF
7
Input
Trigger-IN
TTL
8
GND
GND
Ground
Figure 3-3: SEC2021 Spectrometer Port Pin number

Device User’s Guide 3. System Configuration
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SEC2022 Deuterium Halogen light source
SEC2020 Spectrometer system is equipped with SEC 2022 deuterium halogen light source
as standard content.
Light source specification
Description
SEC2021-025-DUVN
Light type
Deuterium halogen light source
Wavelength range
200 –1700 nm
Stability
< 0.1 %
Drift
< 0.25 % h
Bulb life
> 1000 h (D2 lamp)
> 2000 h (halogen lamp)
Fiber connector
SMA905
Size (W x D x H)
100 x 165 x 46 mm
Figure 3-4: Switch location for the light source

Device User’s Guide 3. System Configuration
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Manual mode:
1. Connect the power cord and turn on the power switch. When the switch is on, the
power LED lights up.
2. Set the auto/manual switch to the left side, the auto/manual LED lights off.
3. Next, slide the D2 switch or halogen switch to the right according to the purpose, and
one or both LED lights up.
4. When the shutter switch is set to the right and the LED lights up, the optical path opens
and it will be in measurement state. When the shutte switch is set to the left and the
LED lights off, the optical path is cut off and it will be setting as dark spectrum state.
Automatic mode:
1. Connect the spectrometer and the light source with the trigger cable.
2. Connect the spectrometer and the PC with USB cable.
3. Turn on the power switch of the light source and slide the auto/manual switch to the
right side, the LED lights up.
4．SpectraSmart controls D2, halogen and shutter operation. Check ON/OFF of each
switch by LED status.
Caution: Turning on the power switch before connecting the ligth source control trigger
cable, it does not work properly. Please be careful to turn on the light source only after
connecting the light source control trigger cable.
Figure 3-5: Connection of the light source control trigger cable

Device User’s Guide 3. System Configuration
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I/O Port Pin
The Pin number of the I/O port for the light source controlling is showing below:
Pin No.
Signal
Definition
Function
1
No connection
2
No connection
3
No connection
4
Input
Shutter
Shutter ON/OFF
5
Input
D2_ON
D2 light sourceON/OFF
6
Input
HAL_ON
Halogen light source ON/OFF
7
No connection
8
GND
GND
Ground
SEC2023 Cuvette holder
It is used to set the cuvettes and plate samples inside. It can also be connected to the
collimator, fiber collimator or optical path, as well as the SMA905 adaptor for light
shielding.
Figure 3-6: Pin number for the deuterium halogen light source
Figure 3-7: Cuvette holder

Device User’s Guide 3. System Configuration
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SEC2024 Platform
Fixing the SEC2021 Spectrometer, 2022 Deuterium halogen light source, and SEC2023
Cuvette holder onto the SEC2024 Platform, it enables a stable installation of the
instrument. (※)
※Fix the devices by screwing the instrument from the side with the platform screw.
Caution! Please be careful to do not lift the device, because there is a possiblity that it
come off, even after fixing.
AC adaptor and Power cable
It is an AC adaptor and Power cable for the SEC2022 Deuterium halogen light source.
Power supply specification
AC input range
100 –240 V
DC output range
12 V (max. 3.34 A)
USB cable
It is used for the connection of the SEC2021 Spectrometer to the PC.
Figure 3-8: Platform for instrument fixing

Device User’s Guide 3. System Configuration
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Collimator
It is used as a fitting when connecting SEC2023 Cuvette holder and SMA905 compatible
optical fiber. And the connected collimator lens can extract light from ultraviolet and
visible light source in parallel. If light is irradiated from the opposite side, it can be
focused on a pinhole. In addition, you can adjust the focal position, by loosening the
fixing screw using the supplied hexagonal wrench and sliding the inner cylinder.
Fiber collimator
Connecting as a joint between SEC2023 Cuvette holder and SEC2021 Spectrometer, light
uptake efficiency is increased. For measurements using cuvette holders, the use of fiber
collimator is recommended. In addition, you can adjust the focal position, by loosening
the fixing screw using the supplied hexagonal wrench and sliding the inner cylinder.
Figure 3-9: Collimator
Figure 3-10: Fiber collimator
Fixing screw
Fixing screw
Fixing
scrww
Fixing screw

Device User’s Guide 3. System Configuration
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External device connection trigger cable
Connecting the SEC2021 Spectrometer and external device with an External device
connection trigger cable, the START/STOP of the spectrometer measurement can be
controlled by measuring operation external device.
Trigger cable
Connecting the SEC 2021 Spectrometer and the SEC 2022 Deuterium halogen light source
with this cable, ON/OFF control of D 2 and halogen lamp and shutter switch can be
controlled by SpectraSmart. Connect the connector with all cords connected to the pin to
the SEC2021 Spectrometer and connect the other connector to the SEC2022 Deuterium
halogen light source.
Also, the two free cords, like the External device connection trigger cable, can control the
spectrometer measurement with the operation of the external devices. Connect the black
cord to the ground of the external device and the brown cord to the trigger out of the
external device.
Figure 3-11: External device connection trigger cable

Device User’s Guide 3. System Configuration
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Plastic cuvette
Cuvette used for absorbance, transmittance, fluorescence and concentration of gas or
liquid samples measurement. Other 10 mm square cuvettes, in glass quartz or similar, can
be for the measurement.
SMA905 adaptor for light source
It is used as a fitting when connecting SEC 2023 cuvette holder and fiber with SMA905
connector.
SMA905 adaptor for light shielding
When measuring with the SEC2023 cuvette holder, it is used to block the external light.
Hexagon wrench
It is used for loosen of the fixing screw attached to the housing of the collimator lens, to
adjust the focal position of the collimator lens. The 0.89 mm wrench corresponds to a
collimator, and the 1.50 mm wrench corresponds to a fiber collimator.
Figure 3-13: SMA905 adaptor for light source
Figure 3-14: SMA905 adaptor for light shielding
Figure 3-12: Trigger cable

Device User’s Guide 3. System Configuration
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Software (USB memory)
Software (SpectraSmart) for the SEC2020 Spectrometer system to be installed.
Quick manual
SEC 2020 Spectrometer system content list, SpectraSmart installation and measurement
setting method are briefly introduced.
Wavelength Calibration Data Sheet
SEC2021 Spectrometer is calibrated at the factory before shipment, and low mercury
lamp is used as the reference light source. In SEC 2020, calibration is carried out with
higher degree polynomials using mercury's representative bright lines.
Linearity Test Data Sheet
Before shipment, the CCD sensitivity of the SEC 2021 spectrometer is not linear with
respect to intensity, and sensitivity varies depending on individual CCDs. Therefore, each
spectrometer is subjected to linearity correction, and the linearity correction table is
stored in each spectrometer.
Waterproof box
Waterproof box for the storage of all contents of the instrument, and can be used for the
easily carry of the system.
Warranty certificate
Based on this warranty certificate, the repair will be free of charges for one year from the
purchase date.

Device User’s Guide 4. Instrument setting for absorbance / transmittance measurement
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4. Instrument setting for absorbance/transmittance measurement
Absorbance is calculated from the original light source spectrum and the spectrum when
the irradiation light of the light source passes through the sample to be measured.
Therefore, to measure the absorbance, first measure the spectrum of the light source,
then set the sample to be measured and measure the light that passed through them.
When the measurement is completed, the program automatically calculates the
absorbance spectrum.
The transmittance is also calculated from the spectrum of the light source and the
spectrum after it has passed through the sample to be measured. When the
measurement is completed, the program automatically calculates the transmittance
spectrum.
Example: 1. Using cuvette
Set the cuvette containing the sample (gas or liquid) and measure the absorbance and
transmittance. Also, by switching to the concentration mode, quantitative analysis of
concentration is also possible.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2022 Deuterium halogen light source
SEC2023 Cuvette holder
SEC2024 Platform
Platform screw
Fiber collimator
SMA905 adaptor for light shielding
Plastic cuvette
Figure 4-1: Absorbance/Transmittance measurement example using cuvette

Device User’s Guide 4. Instrument setting for absorbance / transmittance measurement
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①First, set the spectrometer and light source as shown in Figure4-1. Connect the fiber
collimator to the cuvette holder and connect it to the spectrometer. Fix it with
platform screws on the platform. Connect the SMA905 adaptor for light shielding to
the side of the cuvette holder. When placing the light source on the platform, fix with
the platform screw at the position where the light irradiation port is closest to the
cuvette holder.
②Turn on the light source and turn on the D2 and halogen lamps. Wait from 20 to 30
minutes until the light source stabilize.
③Open the SpectraSmart software, and then open the shutter, set the cuvette
containing solvent or gas to the cuvette holder, and acquire the reference spectrum(※).
④While setting the cuvette, close the shutter (do not turn off the light source),
and acquire the dark spectrum(※).
⑤Open the shutter and set the cuvette containing the sample to be measured
into the cuvette holder and perform the measurement.
※For the acquisition method, please refer to SpectraSmart User's Guide.
Example: 2. Using thin film
Set the thin film in the cuvette holder and measure the absorbance and transmittance.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2022 Deuterium halogen light source
SEC2023 Cuvette holder
SEC2024 Platform
Platform screw
Fiber collimator
SMA905 adaptor for light shielding
Figure 4-2: Absorbance/Transmittance measurement example thin film

Device User’s Guide 4. Instrument setting for absorbance / transmittance measurement
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①Set the instrument in the same way as measurement using cuvette.
②Warming up the light source in the same way as measurement using cuvette.
③Open the SpectraSmart software, and then open the shutter, slide the side of the
cuvette holder and set the reference film or make air as reference spectrum(※).
④Close the shutter and acquire the dark spectrum(※).
⑤Open the shutter, set the thin film to be measured, and perform the measurement.
※For the acquisition method, please refer to SpectraSmart User's Guide.
Example: 3. Using probe
Since immersion probe is measured by immersing the probe directly in the sample,
troublesome sampling is unnecessary. 2, 5, 10, 20 mm optical path length tip are
available.
Configuration example
PC (sold separately)
SEC2021 Spectrometer
SEC2022 Deuterium halogen light source
5.4mm Monocoil all legs, Low Sol 300um
UV, 1.5m Total length with round nut
PD-U10 10mm tip
Figure 4-3: Side of the cuvette holder
Figure 4-4: Absorbance/Transmittance measurement example using transmission dip
probe