Amersham Biosciences Air-900 User manual

1 Introduction
Air sensor Air-900 is a high precision monitor
module for continuous monitoring of air
bubbles in ÄKTA™
design liquid
chromatography systems.
Air bubbles in the capillaries may cause
damage to the column and impairment of the
chromatography result.
The air sensor can be used during sample
application. A valve is switched to a buffer
vessel when bubbles are detected in the
sample line.
The air sensor communicates with the
UNICORN™control system via UniNet 2.
Air-900 consists of a control box and 1-3
flow cells. Two types of flow cells with
different flowpath diameter are available.
Note: The air cell in position 2 is used for
sample application via the sample valve and
sample pump only.
Safety
WARNING! When using hazardous
chemicals, all suitable protective measures,
such as protective glasses, must be taken.
Instructions
Air sensor Air-900
56-3050-85
Edition AC
Flow cell
Air sensor control box

Air sensor Air-900
Instructions 56-3050-85 Edition AC
2
2 Installation
Unpacking
Unpack the module and check the items
against the packing list. Inspect the items for
obvious damage which may have occurred
during transportation.
Installing the air sensor
Connect the air cells and the UniNet 2 cable
to the connectors on the sensor box. Connect
the sensor box to a free UniNet 2 connector
in the ÄKTAdesign system.
If more than one sensor box will be connected
the ID switches must be set as follows:
UniNet 2 cables are used to interconnect the
individual sensor boxes.
Attach the air sensor box vertically to the
mounting slot on an ÄKTAdesign module.
3 Operation
On/Off
The air sensor is on when connected to an
UniNet 2 connector in a running ÄKTAdesign
system.
All interaction with the air sensor is made
through the UNICORN control system.
4 Maintenance
No regular maintenance is required. The only
possible service is cleaning of the flow cell.
Cleaning time < 30 minutes.
A
Fasten
Release
Code 3
1 2
Code 2
1 2
Code 1
1 2
Code 0
1 2
ID switch (black)LED
AIR CELL ID UniNet 2
1 12 23
WARNING! Before connecting the Air
sensor ensure that the power is switched
OFF at the pump or for the complete
system.

5Trouble shooting
Below is a list of possible faults and suggested
actions. If the suggested actions do not
correct the fault, call Amersham Biosciences.
FaultAction
LeakageCheck the tubing and connectors.
Not operating1.Check that the pump is operating.
2.Check the cell function:
-Connect the cell to any
connector on the sensor box.
-Pour liquid into the cell. The
LED shall be green with liquid
and put out with air in the cell.
3.Check that the sensor box is
connected to UniNet 2.
4.Check cables for damage or loose
connections.
5.Check that the ID settings
correspond to the used strategy.
Other faultsContact Amersham Biosciences.
Reference information
Technical specifications
Operating data
Flow rate 0.1 – 100 ml/min
Max Pressure
Cell Air-912
25 MPa (250 bar)
Cell Air-925 2 MPa (20 bar)
Environment +4 to +40 °C
20-95% relative humidity
84-106 kPa (840-1060 mbar)
atmospheric pressure
Physical data
Degree of protection
cell
IP 44
box IP 32
Materials
cell PEEK
box enclosure Aluminum
Chemical resistance
The parts are resistant to
organic solvents and salt buffers
commonly used in
chromatography of biomolecules,
except 100% ethylacetate, 100%
hexane and 100%
tetrahydrofuran (THF)
Power requirement
12–32 VDC via Uninet 2
Power consumption
1,6 W (with 3 cells)
Dimensions,
H x W x D 40 x 140 x 80 mm
incl. attachment 45 x 175 x 85 mm
Weight
cell
0.1 kg
box 0.4 kg (incl. attachment)
EMC Standards
This product meets the requirement of the EMC
Directive 89/336/EEC through the harmonized
standards EN50081-1 (emission) and EN50082-1
(immunity).
Note: The declaration of conformity is valid for the
instrument when it is:
•used in laboratory locations
•used in the same state as it was delivered
from Amersham Biosciences except for
alterations described in the user manual
•connected to other CE labeled Amersham
Biosciences instruments or other products
as recommended.
Accessories and spare parts
Item Quantity Code no.
per pack
Air 900
including one
UniNet 2 cable 1 18-1121-22
UniNet 2 cable, 0.7 m 1 18-1109-74
CellAir-912 (1.2 mm) 1 18-1121-23
Air-925 (2.5 mm) 1 18-1121-24
Air sensor Air-900
Instructions 56-3050-85 Edition AC 3

Important user information
Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Biosciences AB
SE–751 84 Uppsala
Sweden
Trademarks
ÄKTA and UNICORN are trademarks of
Amersham Biosciences Limited or its
subsidiaries.
Amersham and Amersham Biosciences are trademarks
of Amersham plc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Biosciences group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Biosciences UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Biosciences AB
SE-751 84 Uppsala
Sweden
Amersham Biosciences Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855USA
© Amersham Biosciences AB 1998
–All rights reserved
!