AMG EVOS fl User manual

ZP-PKGA-0494 REV A
amgmicro.com
QUICK START GUIDE
Digital Inverted Microscope
for Fluorescence and Transmitted
Light Applications

Overview
The EVOS fl microscope has two types of
controls: mechanical and software. Mechanical
controls include the stage X–Y axis knobs,
focusing knobs, objective selection wheel, and
the light cube selection lever. Software controls
are located in the Control Bar at the bottom of
the display screen. The Channel Bar at the top of
the display screen shows the selected light cube
or transmitted light position.
Avoid exposure to beam
and use protective shields.
NEVER look directly at
UV light!
Basic Operation
1. Turn on the microscope with the power
switch  on the right side of the base.
2. Plug a USB flash drive into one of the
USB ports  on the right side of the
microscope.
3. Place the sample  on the stage, using a
vessel holder if needed.
4. Set magnification with the objective
selection wheel  on the front of the
microscope.
5. Pull the light cube selection lever  (left
side of base) all the way toward the front
of the microscope (the Channel Bar will
highlight the “Transmitted” position).




2
CAUTION
UV Light!
DO NOT Look Directly At Light
Control Bar
Channel Bar


6. Turn on illumination with the LIGHT ON
Button located on the left side of the
control bar.
7. Focus the sample with focusing knobs .
8. Optional: To take a picture of the
transmitted light image, click the Capture
Button on the control bar.
9. Place the light shield box  on the stage,
over the sample.
10. Move the light cube selection lever 
to the desired fluorescence channel (the
Channel Bar will highlight the selected light
cube).
11. With the Find & Focus Tab active, turn on
fluorescence illumination using the LIGHT
ON Button.
12. Adjust the focus as necessary.
13. Adjust the Illumination Intensity Slider on
the control bar as needed.
14. Click the Capture Button.
15. Repeat steps 10–14 to acquire each
fluorescence channel.
16. Click the Overlay Tab to show all channels
in color overlay mode.
17. Adjust Brightness and Contrast for each
channel to bring them into desired balance.
18. Click the Save Button to save the color
image (refer to EVOS fl User Guide).3

Brightness and ContrastOverlay Tab
Save Button
LIGHT ON Button Capture ButtonIllumination Slider

1. Channel Bar (active channel highlighted)
2. Mode Tabs (Find & Focus, Actual, Overlay)
3. LIGHT ON/OFF Button
4. Illumination Intensity Slider
5. Capture Button
6. Boost and Color Options
7. Settings Tab
8. Save Button
9. Information Bar
10. Selected Objective
4
Helpful Tips
 Faint signals near background can be
amplified by turning on the Boost Option.
This only changes the way the image is
displayed; it does not alter the data.
Boost and Color Options
 In Find & Focus or Actual Mode, the Color Option can be turned o to display a grayscale image. This
often shows more details than a color image.
 In Find & Focus Mode, the exposure time is set to 100 ms to assist real-time focusing, moving the
stage, etc. The illumination level is approximately 60% of the amount used for image capture, in order
to minimize photobleaching and phototoxicity. Clicking Capture results in brighter illumination and a
longer exposure time during image capture to provide a high-quality image.
 In Actual Mode, turning on the illumination results in full-powered illumination and actual exposure
times for live viewing of the sample. With longer exposure times (more than 200 ms) there will be a lag
between moving the focus knob and seeing the focus change onscreen.
Note: The EVOS fl User Guide is on the USB flash drive in the accessories box. You can also download it
from the EVOS fl product page at www.amgmicro.com.

       

Power switch Power input jack
USB and DVI
ports
Coarse stage
positioning
knobs
Stage X-axis
knob
Stage Y-axis
knobs
Focusing knob
Phase annuli selector
Condenser slider
slot
Objective
selection wheel
5
Light cube selection lever
(left side of base)

Copyright © 2010 Advanced Microscopy Group. All rights reserved. ZP-PKGA-0494 REV A