AMG EVOS User manual

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Suite 150
Mill Creek, WA 98012
Phone: [phone] or [phone]
Fax: [phone]
E-mail: [e-mail]
Web: www.amgmicro.com
User Guide
Digital Inverted Microscope
The Microcsope Evolved.

© 2008 Advanced Microscopy Group, Inc. All rights reserved.
CONTENTS
EVOS USER GUIDE
GENERAL INFORMATION......................................................................................................................................................23
Environmental Concerns.............................................................................................................................................................................2
Special Precautions.......................................................................................................................................................................................2
Electrical Information...................................................................................................................................................................................2
PLAIN STAGE Components........................................................................................................................................................................3
MECHANICAL STAGE Components........................................................................................................................................................3
SPECIFICATIONS.............................................................................................................................................................................. 4
ASSEMBLY...................................................................................................................................................................................... 56
Stage Plate Assembly...................................................................................................................................................................................5
Plain Stage ...................................................................................................................................................................................................5
Mechanical Stage .....................................................................................................................................................................................5
Changing Stage Plates (Mechanical Stage).........................................................................................................................5
Power Cord Assembly...................................................................................................................................................................................5
Objective Lens Assembly............................................................................................................................................................................6
Filter Slider Assembly...................................................................................................................................................................................6
OPERATION .................................................................................................................................................................................710
Dimmer (Illumination) Control.................................................................................................................................................................7
Illumination Pre-congured for Each Objective .............................................................................................................................7
Learning Function .....................................................................................................................................................................................7
LCD Settings Control.....................................................................................................................................................................................7
Objective Select Wheel................................................................................................................................................................................8
Focus Control...................................................................................................................................................................................................8
Phase Annuli Select Wheel.........................................................................................................................................................................8
Mechanical Stage Operation.....................................................................................................................................................................9
Secure Digital (SD™) Card...........................................................................................................................................................................9
Image Capture Button................................................................................................................................................................................10
Micron(EVOS) via USB Connection .......................................................................................................................................................11
Installing Micron(EVOS) Software......................................................................................................................................................11
Installing Driver........................................................................................................................................................................................11
Working With DNG Files in Micron(EVOS)..........................................................................................................................................12
LCD Positioning ............................................................................................................................................................................................12
MAINTENANCE.......................................................................................................................................................................1314
LCD Screen Care............................................................................................................................................................................................13
Objective Lens Care.....................................................................................................................................................................................13
Stage Care.......................................................................................................................................................................................................13
Firmware Update..........................................................................................................................................................................................14
PARTS & ACCESSORIES..............................................................................................................................................................15
CUSTOMER SERVICE ...................................................................................................................................................................16

2
EVOS™ User Guide
GENERAL INFORMATION
SPECIAL PRECAUTIONS (Figure 2)
When cleaning optical elements, use only materials that are specically made
for that purpose. Further, we recommend soft, low-lint, cloth materials. To
avoid scratching the soft lens coatings, do not use paper-based materials.
Always carry the microscope with one hand supporting the
• base 
and the other hand on the neck , using correct lifting techniques.
Do not exchange objectives between microscopes unless you know that
•
the components have all been approved and recommended by AMG.
If liquid spills on the microscope, turn o the power immediately and
•
wipe dry.
Never disassemble or service the microscope yourself. Unauthorized
•
repairs may damage the microscope or alter its functionality, and they
will void your warranty.
After using, cover the microscope with a dust cover.
•
ENVIRONMENTAL CONCERNS (Figure 1)
Place the microscope on a level surface away from vibrations of other•
pieces of equipment. To protect your microscope from environmental
vibrations, we oer a tabletop Anti-Vibration Platform (part # AMEP-
VIP1) custom designed for EVOS.
Note: Use the 4 adjustable rubber feet on the bottom of the base to
adjust the microscope for uneven surfaces.
Ambient room lighting and sunlight from nearby windows can enter
•
the imaging path and aect the image.
ELECTRICAL INFORMATION (Figure 3)
The• power switch is located on the back of the microscope.
The detachable
• power cord output connection is located next to the
power switch.
Figure 2
Figure 1
 
Figure 3





amgmicro.com 3
EVOS™User Guide
GENERAL INFORMATION
PLAIN STAGE
MECHANICAL STAGE
LCD Display
Plain Stage
Focus Control
LED Lamp House
Objective Select Wheel
Condenser Assembly
Phase Annuli Select Wheel
Image Capture Button
LCD Positioning Knob
LCD Tilt Knob
USB Connect Port
Power Cord
Power Switch
Adjustable Rubber Feet (4)
Secure Digital (SD™) Card Slot
Dimmer
LCD Display
Focus Control
LED Lamp House
Objective Select Wheel
Condenser Assembly
Phase Annuli Select Wheel
Image Capture Button
LCD Positioning Knob
LCD Tilt Knob
USB Connect Port
Power Cord
Power Switch
Secure Digital (SD™) Card Slot
Dimmer
Y-Axis Fine Positioning
X-Axis Fine Positioning
LCD Control
LCD Control
Adjustable Rubber Feet (4)
LCD Settings Button
Power ON Indicator
LCD Settings Button
Power ON Indicator
Coarse Positioning
Note: Install software
before connecting USB.
Note: Install software
before connecting USB.

4
EVOS™ User Guide
SPECIFICATIONS
EVOS SPECIFICATIONS
Optics Innity corrected optical system
Standard Objectives LWD; 4xBF, 10xPh, 20xPh; 40xPh (optional)
Olympus Objectives LWD; 4xPh; 10xPh, 20xPh; 40xPh (optional)
Objective Turret 4-position, with front-mounted control
LCD Display 15”, color, 1024 x 768 pixels, controls for backlight
contrast and saturation; adjustable positioning
Camera 3MP color CMOS
Captured Image Resolution 2048 x 1536 pixels
Captured File Format DNG le format; 3MB/image
Illumination LED, 50,000-hour service life with dimmer
Contrast Methods Brighteld and Phase Contrast
Standard Condenser 3-position turret for BF, 10x/20x Phase and 40x Phase
Olympus Condenser 3-position turret for BF, 4x Phase and 10x/20x/40x
Phase
Filter Assemblies Brighteld: Pinhole and Diuser lters included
Phase: Green lter included
Focusing Dual, low-position controls with rubber grip
PLAIN STAGE 196 mm x 196 mm (7.7” x 7.7”) plain stage;X
low-position, 110 mm (4.3”) from table;X
Working Distance with
Condenser
62 mm (2.4”)X
Dimensions Height: 587 mm (23.1”) with LCD raised to maximum;
Width: 363 mm (14.3”); Depth: 449 mm (17.7”)
MECHANICAL STAGE x-y axis ne-positioning controls;X
low-position, 110 mm (4.3”) from tableX
interchangeable vessel holders available for mostX
common shapes & sizes
110 mm x 110 mm (4.3” x 4.3”) viewable area
X
Working Distance w/ Multi-
well Stage Plate
58.0 mm (2.3”)X
Dimensions Height: 587 mm (23.1”) with LCD raised to maximum;
Width: 363 mm (14.3”); Depth: 449 mm (17.7”)
Weight 11.8 Kg (26 Lb.) - PLAIN STAGE
13.6 Kg (30 Lb.) - MECHANICAL STAGE
Storage Device/Output Secure Digital (SD™) card or USB2 connection to PC
Software Micron(EVOS) imaging software (included)
Power Supply AC Adapter; Input 100-240V, 50-60Hz, 0.5A;
Output 12 VDC
4x: 3.6mm x 2.7mm
10x: 1.44mm x 1.08mm
20x: 0.72mm x 0.54mm
40x: 0.36mm x 0.27mm

amgmicro.com 5
EVOS™User Guide
ASSEMBLY
STAGE PLATE ASSEMBLY
PLAIN STAGE (Figure 1)
The PLAIN STAGE assembly is pre-installed and pre-positioned at AMG.
Simply install the circular stage plate into the round opening in the
stage..
MECHANICAL STAGE (Figures 2 & 3)
For AME i2111/2 and AME 3205/6 Series (MECHANICAL STAGE) microscopes,
follow the procedures below before use.
Screw in the 2
1. coarse positioning knobs into the front right and
left-hand corners of the stage assembly.
Remove the2. stage lock pin on the back right-hand corner.
Note: The stage lock pin is used to secure/hold the mechanical stage
in place when transporting or moving the unit.
CHANGING STAGE PLATES MECHANICAL STAGE (Figure 3)
Unscrew the two 3 mm1. hex screws from the back of the stage with
a hex tool (included).
Unscrew the 22. coarse positioning knobs from the front of the
stage.
Carefully lift the3. stage plate o from the stage assembly and
replace.
Reattach all screws to secure.4.

Figure 2

Figure 3


Figure 1


POWER CORD ASSEMBLY (Figure 4)
Turn the1. power switch to the “” (OFF) position before connecting
the power cord (transformer unit).
Insert the plug into the
2. power outlet on the back of the
microscope.
Insert the 3-prong plug from the power supply into a power socket.
3.
Figure 4



6
EVOS™ User Guide
ASSEMBLY
Figure 3

FILTER SLIDER ASSEMBLY (Figure 3)
Three lter slider assemblies are included with all EVOS microscopes. For
brighteld applications you MUST use the Pinhole or Diuser lter.
Due to variances in sample size, color and thickness, actual lter use may
dier from the standard lter use described below.
Pinhole filter slider assembly• : brightfield, low magnification (4x/10x)
Diffuser filter slider assembly
• : brightfield, high magnification
(10x/20x/40x)
Green filter slider assembly:
• may be used for phase-contrast at all
magnifications (optional)
Note: AMG strongly recommends NOT replacing or tampering with the lens
element as it may cause damage to the lter assembly.
Insert the
1. lter slider assembly into the lter slider slot on the
condenser assembly.
Adjust focus, contrast, and/or illumination and view specimen on
2.
screen.
IMPORTANT!
Use a lter assembly for brighteld.
OBJECTIVE LENS ASSEMBLY (Figure 1 - PLAIN STAGE)
(Figure 2 - MECHANICAL STAGE)
The color-coded objectives match the color-coding label on the objective turret.
When properly installed, EVOS will“know”which objective is in place and adjust
the lighting and image capture parameters to suit.
Carefully remove the1. circular stage plate (PLAIN STAGE) or the
vessel holder (MECHANICAL STAGE) from the stage assembly.
To prevent contamination to optical components, turn the
2. objective
select wheel left 1/2 click, to move the objective turret away
from the optical path, before removing and installing the objective.
Install the objectives into the corresponding positions in the
3. objective
turret located inside the stage assembly (see labels).
Note: Make sure the color coding on the objectives matches the color
coding label on the turret so the automatic settings will work.
Turn the
4. objective select wheel to switch between objectives.
Please note that EVOS models AME-3201 and AME-3204 are congured for use
specically with the following objectives:
4x Phase Contrast, AMG part #AMEP-4602 (Olympus part #1U2C522P)
10x Phase Contrast, AMG part #AMEP-4603 (Olympus part #1U2C144P)
20x Phase Contrast, AMG part #AMEP-4604 (Olympus part #1U2C146P)
40x Phase Contrast, AMG part #AMEP-4605 (Olympus part #1U2C149P)
Use with any other objectives besides those listed above will void AMG’s warranty
support for your EVOS model AME 3201 or AME 3204 microscope.
Figure 2
Figure 1





amgmicro.com 7
EVOS™User Guide
OPERATION
DIMMER ILLUMINATION CONTROL (Figure 1)
Turn the1. power switch to the “|”(ON) position.
Rotate the
2. dimmer control located on the bottom right-hand side
of the microscope base to adjust brightness/intensity.
Illumination Pre-congured for Each Objective
The illumination/brightness levels on EVOS microscopes are precongured
for each individual objective for optimal performance. The microscope is
set to recognize each objective automatically as the objective select wheel
rotates.
Learning Function
EVOS microscopes are equipped with a memory function that remembers
the last automatic or manual illumination setting that was used for each
individual objective, EVEN AFTER BEING POWERED OFF. As the objectives are
switched, illumination values return to the last set levels for each objective.
Figure 1

LCD SETTINGS CONTROL (Figures 2 & 3)
The LCD settings button gives the user direct control for the following
LCD display settings:
BACKLIGHT
X
CONTRASTX
SATURATIONX
Press the1. LCD settings button to initiate settings control mode.
Turn the
2. LCD control to adjust the setting (8 levels available) to
desired view.
When nished, wait approximately 4 seconds for the settings panel to3.
close.
Figure 2

Figure 3


8
EVOS™ User Guide
OPERATION
OBJECTIVE SELECT WHEEL (Figure 1)
Rotate the1. objective select wheel located at the bottom center of
the microscope base until a desired objective is in position (see label).
The microscope will automatically recognize each individual2.
objective and use the pre-congured illumination settings for optimal
performance.
Rotate the3. phase annuli select wheel to the position that
corresponds with the respective objective.
FOCUS CONTROL (Figure 2)
Focus control1. knobs are located on both sides of the microscope.
Backward rotation will move the objective away from the specimen
and forward rotation will move it toward the specimen.
Rotate as necessary to adjust focus of the specimen on screen.
2.
Note: Every EVOS microscope has a safety feature that incorporates a
“slip-clutch” mechanism to protect the objectives. When the objective
turret reaches the end of its travel range, the clutch makes a “clicking”
sound as the focus control continues to turn. The objective does not
move past the acceptable range.
PHASE ANNULI SELECT WHEEL (Figure 3)
Phase rings are pre-installed and pre-aligned on the phase annuli select
wheel for optimal performance. Realignment or re-centering is not
required. For all service needs, refer to the Customer Service section.
EVOS microscopes have 3 positions for phase contrast:
STANDARD OPTICS OLYMPUS OPTICS
brighteld (BF) brighteld (BF)
X
10/20x phase 4x phaseX
40x phase 10x/20x/40x phaseX
Note: View through the small window in the front of the phase condenser
assembly to set the phase annuli to its respective objective. The phase setting
must correspond to the objective for proper operation.
Figure 1

Figure 2

Figure 3



amgmicro.com 9
EVOS™User Guide
OPERATION
MECHANICAL STAGE OPERATION (Figure 1)
The free-motion “glide”stage operates and moves in all directions —
horizontally, vertically and diagonally.
Maneuver the stage freely throughout its x-y range of motion using
1.
the 2 coarse-positioning knobs .
Turn either of the 2
2. y-axis ne-positioning controls toward the
front to move the stage forward and toward the back to move the
stage backward.
Turn the
3. x-axis ne-positioning control counter-clockwise to
move the stage to the left and clockwise to move the stage to the
right.
SECURE DIGITAL SD™ CARD (Figures 2 & 3)
The SD memory card slot is located on the top of the LCD display.
Note: Before using the SD card, format the disk/card by following the
manufacturer’s format disk procedures.
Gently pull out the
1. protective rubber cover from the LCD frame.
Insert the
2. SD card with the angled corner facing bottom left-hand
side (see Figure 3) into the slot until it clicks and the card locks into
position.
Remove the card by pressing it down to release it from the slot.
3.
Note: Do not remove SD card until “STORING: EVOS____.DNG” clears
from the LCD screen



Figure 1
Figure 2

Card
Figure 3


