ANDalyze AND1100 User manual

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Document:AND‐Prod‐1100‐8‐2013
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AND1100Fluorimeter
forWaterTesting
UserManual
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August,2013Edition2
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©ANDalyze,Inc.,2011.Allrightsreserved.PrintedinUS
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AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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TableofContents
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2SafetyInformation……………………………………………….…………………………………………………………………3
2.1UseofHazardInformation
2.2Precautionarylabels
2.3ChemicalandBiologicalSafety
3GeneralInformation…………………..…………………………………………………………………………………………4
3.1OverviewofProduct
3.2GeneralFeatures
3.3AnalyteSensors
3.4OperatingEnvironment&Storage
3.5WaterTestingGuidelines
4Set‐UpandOperatingProcedures…………………………………………………………………………………………6
4.1ChargingtheBattery
4.2HomeTestingScreen
4.3SensorScreen
4.4SiteScreen
4.5StartOption
4.6MenuScreen
4.7FirmwareUpdate
4.8InstraCommLite
5SiteSet‐UpandManagement.……………………………………………………………………………………………….11
5.1NewSiteSet‐Up
5.2On‐SiteCalibration
5.3SelectingaSite
5.4DeletingaSite
5.5Re‐CalibratingaSite
6Maintenance……………………………………………………………………………………………………………………….…13
7CustomerServiceContactInformation…………………………………………………………………………………..14
8ConsumablesandReplacementItems……………………………………………………………………………………14
9TechnicalSpecificationforAND1100Fluorimeter………………………………………………………………….15
10LimitedWarranty…………………………………………………………………………………………………………………16

AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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2SafetyInformation
Pleasereadthisentiremanualbeforeunpacking,settinguporoperatingthisequipment.Payattentiontoall
danger,warningandcautionstatements.Failuretodosocouldresultinseriousinjurytotheoperatoror
damagetotheequipment.Makesurethattheprotectionprovidedbythisequipmentisnotimpaired,donot
useorinstallthisequipmentinanymannerotherthanthatspecifiedinthismanual.Iftheequipmentisused
inamannernotspecifiedbythemanufacturer,theprotectionprovidedbythemanufacturermaybeimpaired.
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2.1UseofHazardInformation
DANGER:
Indicatesapotentiallyorimminentlyhazardoussituationwhich,ifnotavoided,couldresultindeathor
seriousinjury.
CAUTION:
Indicatesapotentiallyhazardoussituationthatmayresultinminorormoderateinjury.
Important:
Indicatesasituationwhich,ifnotavoided,maycausedamagetotheinstrument.Informationthatrequires
specialemphasis.
Note:
Informationthatsupplementspointsinthemaintext.
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2.2PrecautionaryLabels
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Readalllabelsandtagsattachedtotheinstrument.Personalinjuryordamagetotheinstrumentcouldoccurif
notobserved.Asymbol,ifnotedontheinstrument,willbeincludedwithadangerorcautionstatementinthe
manual.Thissymbol,ifnotedontheinstrument,referencestheinstructionmanualforoperationand/orsafety
information.ElectricalequipmentmarkedwiththissymbolmaynotbedisposedofinEuropeanpublicdisposal
systemsafter12Augustof2005.InconformitywithEuropeanlocalandnationalregulations(EUDirective
2002/96/EC),Europeanelectricalequipmentusersmustnowreturnoldorend‐oflifeequipmenttothe
manufacturerfordisposalatnochargetotheuser.
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Note:Forreturnforrecycling,pleasecontacttheequipmentproducerorsupplierforinstructionsonhowto
returnend‐of‐lifeequipment,producer‐suppliedelectricalaccessoriesandallauxiliaryitemsforproper
disposal.
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

AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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2.3ChemicalandBiologicalSafety
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DANGER:PotentialChemical/BiologicalExposureHazards.Handlingchemicalsamples,standardsandreagents
canbedangerous.Usersofthisproductareadvisedtofamiliarizethemselveswithsafetyproceduresandthe
correctuseofchemicals,andtocarefullyreadallrelevantMaterialSafetyDataSheets.
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‐Normaloperationofthisinstrumentmayinvolvetheuseofhazardouschemicalsorbiologicallyharmful
samples.
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‐Theusermustobserveallcautionaryinformationprintedontheoriginalsolutioncontainersandsafety
datasheetpriortotheiruse.
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‐Allwastesolutionsmustbedisposedinaccordancewithlocalandnationallaw.
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‐Thetypeofprotectiveequipmentmustbeselectedaccordingtotheconcentrationandamountofthe
dangeroussubstanceatthespecificworkplace.
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3GeneralInformation
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3.1OverviewofProduct
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TheAND1100™Fluorimeterenablesfieldtestingtobedoneintwoshortsteps,
eliminatingorsignificantlyreducingtestexpenditures,complexityandwaittimes
experiencedintraditionalheavymetalswatertesting.TheFluorimeterusesan
extremelysensitivemeasurementtechniquetodeterminethelevelsofheavymetal
contaminants.ANDalyze’sproprietaryCatalyticDNAsensorsuseaDNAzymereaction
thatfluorescesinthepresenceofatargetcontaminantsubstancesuchaslead.The
fluorescenceofthereactionismeasuredtodeterminetheconcentrationofthe
targetheavymetalandisreportedinpartsperbillion(ppb).Usingtheproducttotest
formetalsisasimple,quantitativetestthatallowsfordetectionatorbelowUSEPA
standardsindrinkingwater.Thetestisperformedbytakinga1milliliterwater
sample,injectingitthroughthesensor,andintotheAND1100fluorimeter.This
sampleisthenautomaticallyanalyzedandreportsresultsinlessthantwominutes.
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

AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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3.2GeneralFeatures
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‐FluorescenceBasedSensing‐Reactionproducesquantitativefluorescencebasedresults.
‐SensorKit‐Eachdisposablecolor‐codedsensorisdesignedforaspecificheavymetaltesttarget.Kits
providesamplingtubes,syringesandcuvettes.
‐Rugged&ConvenientConstruction‐Tough,lightweight,impact‐resistantshellprotectselectronicsand
sensors.
‐KeyPad‐Quick,simplemenunavigationandonebuttonpushforsampleanalysis.
‐DataCapture&Reporting‐Timeanddatestampedwithsamplenumber.Storesover240,000testresults
andover75sitelocations.DatadownloadablethroughaUSBcable.
‐WaterandDustResistant‐Enclosurerating‐IP54
‐Portable‐BatteryoperatedandrechargeablethroughUSBcableortraditionaladapter.
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Note:Seefulltechnicalspecificationsinsection9.
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3.3AnalyteSensors
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Tohelpdifferentiatethematerialsspecifictoeachanalyte,thepackaginghasbeenindividuallylabeledandthe
sensorhousingsandbuffercapshavebeencolorcoded.ForExample:

Green–Lead(Pb)Orange–Uranium(U)Blue–Copper(Cu)Gray–Mercury(Hg)Etc.

Note:Insomecasesthesamesensorisusedformultiplerangesbutdifferentbuffersand/ormethodswill
accompanythesensorinthekit.
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3.4OperatingEnvironmentandStorage
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Important:Thefollowingconditionsarenecessarytoensurecorrectinstrumentoperationandaccurate
results:

‐Placetheinstrumentfirmlyonaflatandevensurfaceduringoperation.
‐Maintainanambienttemperatureof10to40ºC(50to104ºF)forproperfluorimeteroperation.Pleasesee
themanualforeachspecificmetalforsensorspecifictemperatureconditions.
‐Therelativehumidityshouldbelessthan80%;moistureshouldnotcondenseontheinstrument.
‐Donotoperateorstoretheinstrumentinextremelydusty,damporwetlocations.
‐Keepthesurfaceoftheinstrument,thecellcompartmentandallaccessoriescleananddry.
‐Sensorsarestableupto1yearifstoredat<23˚C(74˚F),<50%R.H.awayfromdirectsunlight.Forbest
results,storeintherefrigerator(4˚C/39˚F).
‐Buffersolutionsarestableupto6monthsifstoredat<23˚C(74˚F)butifstoredintherefrigerator
(4˚C/39˚F),canbeusedforupto1year.
‐Sensorsandbuffersmustbebroughttoroomtemperaturebeforeuse.

AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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3.5WaterTestingGuidelines

Note:Thisisadrinkingwatertestkit.Ifyouaretestingothermatricessuchassurfacewater,groundwateror
industrialwater;contactANDalyzecustomerserviceforadditionalapplicationnotes.

WaterSampling
‐Forbestresultsusefreshlycollectedsample(unpreserved)foranalysis.Werecommendthatyouusethe
samplewithin1hour(maximumof2hours)ofcollectiontominimizeanymetallosstothewallsofthe
samplecontainer.Thisisparticularlyimportantfortestingtraceleadlevels.
‐OncethesampleismixedwithANDalyzesamplebuffer,testwithin15minutes.
pHRange
‐TestshaveshownthatenvironmentalsamplespreservedinacidtoapH<2cannotusuallybebroughtto
anappropriatepHwhenmixedwiththeANDalyzebuffer.Thesesamplesmustbefirstneutralizedwith
NaOHtoasuitablepHbeforemixingwithANDalyzebuffer.PleasecontactANDalyzecustomerservicefor
instructionsrelatedtopre‐treatmentofhighlyacidicorhighlybasicsamples.

4Set‐UpandOperatingProcedures
4.1ChargingtheBattery

Important:Beforeusingtheinstrumentassurethatthefluorimeterdeviceischarged.

ToConfirmtheBatteryLevel:
1. TurnPowerONbypressingandHoldingtheON/OFFbutton.(See
HorizontalArrowonPhototoRight)

2. Oncedeviceisinitiated,confirmthebatteryindicatorstatusisgreen
(SeeVerticalArrowonPhototoRight).Thebatteryindicatorshouldnot
bered(asseeninthephototoright)

ChargingtheBattery:
1. ReleasetheRubberBootatthebottomofthemeter.

2. AttachtheUSBtoMINI‐BCabletotheoutletatthebottomofthe
deviceandtoaUSBpoweredsourcesuchasacomputeroraUSB
charger.(SeePhototoRight).

Note:Whenthemeterischarging,thebatteryindicatorwilldisplayyellow
linesacrossandaroundtheindicator.





AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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4.2HomeTestingScreen
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Optionscanbeaccessedby:
‐Usingtheup/downbuttonsandpressingSELECTbuttonwhenthearrowistotheleftofthedesiredoption.
‐ORbypressingthebuttonlocatedjustbelowthescreenunderthedesiredoptionsatbottom.
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SelectableOptions:
1) Sensor‐SelectswhichSensorwillbetested.
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2) Site‐Usessitespecificcalibrationsforaccuratetests.
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3) Start‐Tobeginatestoftheindicatedmetal.
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4) Menu‐Tochangeorviewinternalsettings.
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4.3SensorScreen
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SelectingtheSensoroptionallowstheusertochangethesensor
whichthemeterwilldetect.
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Onceselected,theColoroftheareasurroundingthenameof
thesensorandthephotoofthesensorattherightontheHome
Screenwillmatchthecolorofthesensoritselfusedfortesting.
4.4SiteScreen
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SelectingtheSiteoptionallowstheusertochangeorcreatenewsites.
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Sitesareimportantbecauseeachwatersamplecontainsdifferences
whichaffecttestingresults.Itisrecommendedthateachlocationis
calibrated.Sobyselectingasite,thiscalibrationcanbeusedrepeatedly.
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Pleaseseesection5belowforfurtherinstructionsonsettingupandcalibratingsites.
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4.5StartOption
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DependingonyourOptionssettings,
pressingtheStartbuttonwilleither
startthetestinginstructionsOR,if
turnedOFF,willbeginthetesting
immediately(foradvancedusers).
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AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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4.6MenuScreen
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TheMenuScreencanbeaccessedbypressingthebuttonlocatedbelowthescreen
underMenu.
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SelectableOptions:
1. Results
2. Settings
3. Options
4. Advanced
5. About
6. Date/Time
7. Help
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OptionsareselectableusingthearrowkeysandpressingSelectbuttonorby
pressingthebuttonlocatedbelowthescreenundertheSelect.
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Results
‐Displaystheresultsofthepreviousfourtests.
‐Scrollupordowntoviewadditionalresults
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Settings
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‐Units:Determinesunitsfordisplayingmetal
concentration.Youcanchangethistoppb,ppm,
µMornM.
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‐Minppb:Atconcentrationslessthenminppb,
“BelowLimit”isdisplayed.
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‐Maxppb:Atconcentrationsgreaterthanmax
ppb,“AboveMax”isdisplayed.
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‐Decimals:Changesthenumberofdecimals
displayedintheresults.Undersome
circumstancesthedecimalswillbedefaulted.
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Note:TheDefaultvaluesarethesettings
recommendedbythemanufacturer.
About
‐DisplaystheHardwareandFirmwareversions
ofthemeterincludingthemostrecentdate
thefirmwarewasupdated.
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Note:Updatefirmwareifithasnotbeendone
recently.
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DateandTime
‐Setthedateandtimetomaintainaccurate
history.EditusingthearrowkeysandSave
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Help
‐DisplaysHow‐Tovideoshowingasample
calibrationorprovideslinktoviewvideos
online.

AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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Options
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PowerSave:Thissetstheminuteswhenthedisplay
willturnblacktosavepower.Settablefrom1to5
minutes.
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PowerOff:Thisistheminuteswhichtheunitwill
turnitselfoffafteritgoesintopowersavemode.
Settablefrom6to15minutes.
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CuvetteRemoval:TurningthisONwillcausea
screentoappearaftereachtestremindingtheuser
toremovethecuvettefromthesamplechamber.
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TestInstructions:IntheONposition,4‐step
instructionswilldisplayinorderwhenTestis
selectedfromthehomescreen.Afterthe4thstep,
thetestwillbegin.IntheOFFposition,thetesting
beginsimmediately.
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SensorLot:TurningthisoptionONallowstheuserto
enterthelotnumberofthesensorsatthetimeofa
sitecalibration.Thishelpstoidentifywhenanewsite
calibrationisrequired(newlotbatch).
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Temperature:TurningthisoptionONallowstheuser
toenterthetemperatureofthesamplewateratthe
timeofasitecalibration.Thishelpstoidentifywhena
newsitecalibrationisrequired(significantchangein
watertemperature).
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TemperatureUnits:SwitchbetweenCelsiusand
Fahrenheitfortemperatureentryanddisplays.
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DisplayContrast:Adjuststhebrightnessofthedisplay.
Highervalueindicatesabrighterdisplay.
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Language:ChoosebetweenEnglish,Chinese,
Portuguese,SpanishandGermanlanguagesforthe
fluorimeterdisplays.
Advanced
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UsercanViewandSetparametersforeachspecificsensor.Edit
valuesusingup/downkeysandSELECT.
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Window:Determinesthenumberofpointsthatwillbeusedto
calculatetherateoffluorescenceincreaseovertime.Weusea
movingaveragemethodtocalculatemaximumrate.(Ifthevalueof
windowandsamplesissame,thentheaverageslopeiscalculated)
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Samples:Totaldatapointstakenwhichdeterminestimeuptowhich
dataiscollected.Avalueof100isapproximately20seconds.Increase
samplevalue,toincreasedatacollectiontime.Generallyanincrease
insamplescanincreasesensitivityespeciallyinmatricessuchaswaste
water.
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AND1100FluorimeterforWaterTestingJuly1,2013


ANDalyzeInc.800BoylstonStreet,PrudentialTower,Floor16,Boston,MA02199USATel.+1857.453.6740www.andalyze.com
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Scale:Slopeofthelinethatcorrelatestherateoffluorescence
increasevs.concentration.Thisvalueisdeterminedbythe3‐
pointcalibration.Somesensorshaveadefaultscalethatis
automaticallycalculatedfromothersensor’sscales.Inthis
case,“Auto”willbeindicatedinthedefaultscalefield.Anew
3‐pointcalibrationwillsupersedethedefaultsettings.
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Offset:Interceptofthelinethatcorrelatestherateof
fluorescenceincreasevs.concentration.Alsoautomatically
determinedbythe3‐pointcalibration.
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Delay:Pointsfromthestartofthetestwhich
arenotusedforcalculations.Thisisused
whenthereisadelayedreactionincertain
sensors.
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R2:R‐Squaredisastatisticaltermindicating
howgoodonetermisatpredictinganother.In
ourcase,thisisderivedfromthebestfitline
createdbythe3‐pointlabcalibration.Values
closerto1arebetter.
3‐PointLabCalibration

Usingtheleft/rightkeysinthe
Advancedmenu,Selectthe
Calibrateoption.


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Whenall(3)solutionshavebeen
tested,thecalibrationresults
screenwillbedisplayed.Click
Applytosavetheresults.The
settingswillthenbeviewablein
theAdvancedscreen.

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Note:Thisisforlaboratory
calibrationONLYand
requires(3)standardized
solutionsfrom
manufacturerorprepared
byalaboratorytechnician.
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FactoryReset

TheResetoptionwillrestorethesettingofthat
specificsensortofactorysettings.

Usingtheleft/rightkeysintheAdvancedmenu,
SelecttheResetoption.



SelectResetontheWarningscreentocomplete
theprocess.
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4.7FirmwareUpdate
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ANDalyzeiscontinuallyaddingnewfeaturesandoptionstotheAND1100throughnewfirmwareupdates.To
updateyourAND1100firmware,pleasevisitandalyze.comandlogintoregisteryourdevice.Onceloggedin,
youwillseetheoptiontoupdateyourdevice.Followtheinstructions.
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