Antec DECADE Elite User manual

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Antec
Industrieweg 12
2382 NV
Zoeterwoude
T
he Netherlands
DECADE Elite
User Manual
175.0010, Edition 2, 2015

2
Copyright ©2015, Antec, The Netherlands. Contents of this publication may not be reproduced in any form or by any means
(including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent
from the copyright of the owner. Antec reserves the right to make changes to the design and specifications of the instrument
and this manual without prior notice.
ROXY, ALEXYS, DECADE, DECADE II, DECADE Elite, DECADE Lite, INTRO, Flexcell, ReactorCell, ISAAC, HyREF, Sen-
Cell and SynthesisCell are trademarks of Antec. Whatman™ (word and device) and Whatrnan™ (word only) are trademarks
of Whatman lnternational Ltd. SOLVENT IFD™ and AQUEOUS IFD™ are trademarks of Arbor Technologies, Inc. Clarity®,
DataApex® are trademarks of DataApex Ltd. Microsoft® and Windows™ are trademarks of Microsoft Corporation. Excel is a
registered trademark of the Microsoft Corporation. All other trademarks are the property of their respective owners.
The software and the information provided herein is believed to be reliable. Antec shall not be liable for errors contained
herein or for incidental or consequential damages in connection with the furnishing, performance, or use of software or this
manual. All use of the software shall be entirely at the user’s own risk.

Declaration of conformity
We Antec Leyden B.V., Zoeterwoude, The Netherlands, declare that the product:
DECADE Elite™ Electrochemical Detector type 175 and 176
to which this declaration relates, is in conformity with the following CE directives:
Low Voltage Directive ( 2006/95/EC)
Safety requirements for electrical equipment for measurement, control, and laboratory use:
- Part 1: General Requirements
- Part 2-010: Particular requirements for laboratory
equipment for the heating of materials
IEC61010-1 3ed:2010
IEC61010-2-010 3ed:2014
EMC Directive (2004/108/EC)
Electrical equipment for measurement, control and laboratory use - EMC requirements
- Part 1: General Requirements IEC61326-1 2ed: 2012
Electromagnetic Compatibility (CISPR11: group 1, Class A)
Group 1 ISM equipment: group 1 contains all ISM equipment in which there is intentionally generated and/or
used conductively coupled radio- frequency energy which is necessary for the internal functioning of the equip-
ment itself.
Class A equipment: is equipment suitable for use in all establishments other than domestic and those directly
connected to a low voltage power supply network which supplies buildings used for domestic purposes.
WEEE directive (2002/96/EC) Waste Electrical and Electronic Equipment
RoHS directive (2011/65/EU) Restriction of Hazardous Substances
This device complies with the requirements of CISPR11 group 1 class A ISM equipment. In a
domestic environment this product may cause radio interference in which case the user may be
required to take adequate measures on his own expenses. Only use manufacturer-supplied
cable(s) to connect with other devices. Thoroughly connect shielding to common. Manufacturer
will not accept any liability for damage, direct or indirect, caused by connecting this instrument
to devices and with cables which do not meet relevant safety standards.
Zoeterwoude,
The Netherlands
March 2, 2015

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Table of contents
Table of contents 4
Warning Symbols 7
Safety instructions 9
Introduction 13
Instrument description 14
Installation 17
Site Preparation Requirements 17
Unpacking 18
Mains connection 20
PC connection 21
Software 24
HPLC fluid connections 29
Maintenance & Shutdown 37
Maintenance 37
Shutting down the system 39
DECADE Elite controller 41
Introduction 41
Overview of DECADE Elite screens 42
Parameters 47
Clarity DECADE Elite control module 51
Detection and Parameters 55
Introduction 55
Three-electrode configuration 55
Internal organization 57
Dual flow cell control 57
Filter 62
Introduction 75
Hydrodynamic and scanning voltammogram 83
Specifications DECADE Elite 93
Rear panel I/O 97
USB B connector 98
LAN connector 98
VALVE connector 98
ANALOG DATA connector 102
Digital I/O connector 104
Troubleshooting 109
Instrument errors 109
Analytical troubleshooting 111
Dummy cell test 112
Stop flow test 113
Detector accessories 119
Index 120

CHAPTER 1 Table of contents 5
Intended use
The DECADE Elite Electrochemical Detector is used in combination with
(Ultra) High Performance Liquid Chromatography for the electrochemical
detection of suitable analytes in liquid samples. With this technique the
amount of electroactive substances in mobile phase can be quantified. The
instrument can be used for the chromatographic analysis of a wide range
of electroactive analytes in the fields of for example:
•Bioanalytical analyses
•Food analyses
•Environmental analyses
Operation of an electrochemical detector can involve the use of hazardous
materials including corrosive fluids and flammable liquids. The instrument
should only be operated by users with the following expertise:
•Completed degree as chemical laboratory technician or compara-
ble vocational training
•Fundamental knowledge of liquid chromatography
•Participation in an installation of the system performed by the man-
ufacturer or a company authorized by the manufacturer and suitable train-
ing on the system and chromatography software.
•Knowledge and experience in the safe handling of toxic and corro-
sive chemicals and knowledge of the application of fire prevention
measures prescribed for laboratories.
Information on safety practices is provided with your instrument and opera-
tion manuals. Before using your instrument or accessories, you must thor-
oughly read these safety practices. This manual is written for laboratory
technicians who use the DECADE Elite detector for (U)HPLC analysis.
Unskilled, improper, or careless use of this instrument can create fire
hazards, or other hazards which can cause death, serious injury to
personnel, or severe damage to equipment and property. Observe all
relevant safety practices at all times. Only use the device for
applications that fall within the scope of the specified intended use.
Else the protective and safety equipment of the device could fail.
For research purposes only. While clinical applications may be shown,
this instrument is not tested by the manufacturer to comply with the In
Vitro Diagnostics Directive.

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WEEE directive
All equipment of Antec Leyden which are subjected to the WEEE directive
shipped after August 13, 2005 are compliant with the WEEE marking re-
quirements. Such products are labelled with the “crossed out wheelie”, de-
picted on the left site.
The symbol on the product indicates that the product must not be
disposed as unsorted municipality waste.
Collection & recycling information
Please ship the instrument back to the manufacturer (Antec Leyden,
the Netherlands) at the end-of-life time of the product. The manufac-
turer will take care of the proper disposal and recycling of the instru-
ment at its facilities.
Shipping address for the end-of-life products:
Antec Leyden B.V.
Industrieweg 12
2382NV Zoeterwoude
The Netherlands
In case of questions, or if further information is required about the collec-
tion & recycling procedure, please contact your local distributor.
ROHS directive
The DECADE Elite is ROHS compliant and in conformity with Directive
2011/65/EU Restricted use of Hazardous Substances in electrical and
electronic Equipment (ROHS).
Antec Leyden is an ISO 9001:2008 certified company.

CHAPTER 1 Table of contents 7
Warning Symbols
The following symbols are used in this guide:
This sign warns about the risk of electric shock. It calls
attention to a procedure or practice which, if not adhered
to, could result in loss of life by electrocution. Do not pro-
ceed beyond a danger sign until the indicated conditions
are fully understood and met.
The warning sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in severe injury, loss of life or damage or destruction
of parts or all of the equipment. Do not proceed beyond a
warning sign until the indicated conditions are fully under-
stood and met.
The caution sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could re-
sult in damage or destruction of parts or all of the equip-
ment and/or erratic results. Do not proceed beyond a
cautions sign until the indicated conditions are fully un-
derstood and met.
The biohazard sign draws attention to the fact that use
of biological materials, viral samples may carry a signifi-
cant health risk.
The toxic hazard sign draws attention to the fact that use
of toxic solvents or samples may carry a significant
health risk.

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The attention sign signals relevant information. Read this
information.
The note sign signals additional information. It provides
advice or a suggestion that may support you in using the
equipment.

CHAPTER 1 Table of contents 9
Safety instructions
Adhere to the following standard quality control procedures and the follow-
ing equipment guidelines when using the DECADE Elite detector. The fol-
lowing safety practices are intended to ensure safe operation of the instru-
ment.
Working environment & safety
The intended use of the instrument is to detect electroactive substances in
liquid samples in combination with a (U) HPLC system in a GLP-approved
environment. Operators using the system should have the appropriate ed-
ucation an extensive understanding of GLP rules and be skilled in the art.
Use this system ONLY for the intended use. Use of the system for any
other purpose will cause unsafe situations.
System Operation
To assure optimal performance keep of the detector we recommend that
the instrument is checked regularly and maintenance procedures are car-
ried out. Preventive maintenance contracts are available for that
Purpose. Please contact your local dealer or the nearest sales office for
more information.
Electrical safety
The removal of protective panels on the instrument can result in exposure
to potentially dangerous voltages. Therefore, disconnect the instrument
from all power sources before disassembly.
Untrained personnel should not open the instrument, this may only be
done by authorized service engineers. Replace or repair faulty insula-
tion on power cords immediately after discovery of the fault. Check that the
actual power voltage is the same as the voltage for which the instruments
are wired. Make sure power cords are connected to correct voltage
sources: grounded AC power source, line voltage 100 –240 VAC. The in-
strument should be connected to a protective earth via a ground socket.
The DECADE Elite must only be used with appliances and power sources

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with proper protective grounding to prevent damage through build-up of
static electricity. The power source should exhibit minimal power transients
and fluctuations. If necessary connect to a filtered mains socket.
Replace blown fuses with fuses of proper type and rating as indicated on
the rear panel and as listed in the list of accessories and spares (appendix
D). The fuse holder is integrated in the mains connector. Ensure that the
instrument is never put in operation with fuses of a different type. This
could cause fire. Only use manufacturer-supplied I/O cable(s) to connect
with other devices. Thoroughly connect shielding to common. Manufac-
turer will not accept any liability for damage, direct or indirect, caused by
connecting this instrument to devices and with cables which do not meet
relevant safety standards.
Solvents
The solvents used may be flammable, toxic or corrosive. The room in
which the system is installed should be well ventilated to prevent that sol-
vent vapors cause poisoning or ignite and cause a fire. Use of open fire in
the vicinity of this system must be strictly prohibited. Do not install the sys-
tem in the same room with any other equipment that emits or could poten-
tially emit sparks. Provide protective equipment near the instrument, when
solvent gets into the eyes or on the skin, it must be flushed away immedi-
ately. Provide equipment, such eye wash stations and safety showers, as
close to system as possible. Use proper eye and skin protection when
working with solvents. Additional safety requirements or protection may be
necessary depending on the chemicals used in combination with this
equipment. Make sure that you understand the hazards associated with
the chemicals used and take appropriate measures with regards to safety
and protection.
Sample containers (vials) should be sealed to minimize any risks related to
solvent vapor.
USE ONLY WITH 250 V FUSES
EMPLOYER UNIQUEMENT
AVEC DES FUSIBLES DE 250V