anvajo fluidlab R-300 User manual

User manual
fluidlab R-300

User Manual fluidlab R-300
Edition 202104 3
Vers.: 1.2
©Copyright 2018 - Copyright Notice
All the contents of this user manual, in particular text, photographs and graphics, are
protected by copyright. Unless expressly stated otherwise, the copyright is held by
anvajo GmbH. Please ask us if you wish to use the contents of this user manual
elsewhere.
Any person infringing this copyright (for example, by copying images or text without
permission) is committing an offence in accordance with Sections 106 ff. of the
German Copyright Act and may be liable for damages (Section 97 of the Copyright Act).
Registered Trademarks
All hardware and software names specified in this manual are registered trademarks or
should be regarded as such. The proper names of anvajo products and software are
shown in bold.
Errors
Great care has been taken in compiling the text and figures in this manual. Nonetheless,
the possibility of errors cannot be fully excluded. The company accepts no legal
responsibility or liability of any kind for incorrect information and the consequences
thereof. The company is always grateful for any suggestions for improvement and
notifications of errors.
Gender note:
To improve readability, the masculine form has been used in the text, but the
information refers to members of all genders.

User Manual fluidlab R-300
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User Manual fluidlab R-300
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1 Contents
1Contents ................................................................................................................................ 5
2Safety Instructions/Hazard Analysis............................................................................... 7
2.1 Symbols Used in the User Manual and on the Device.............................................. 7
2.2 General Safety Instructions............................................................................................ 8
2.3 Electromagnetic Considerations.................................................................................... 9
2.4 Lithium Polymer Battery ...............................................................................................10
2.5 Radio Communications..................................................................................................10
2.6 Protection Against Ultraviolet Radiation (UV) ..........................................................11
3Introduction ........................................................................................................................13
3.1 A Brief Description and Intended Purpose ...............................................................13
3.2 Overview..........................................................................................................................14
3.3 Product Set Components..............................................................................................15
3.4 Description of Device....................................................................................................16
4Setup and Use.....................................................................................................................17
4.1 Initial Setup ......................................................................................................................17
4.1 Charging the Built-in Battery .......................................................................................17
4.2 Switching On the Device ..............................................................................................18
4.3 Switching Off the Device..............................................................................................18
4.4 Exiting Energy-saving Mode.........................................................................................19
5Setting up the System.......................................................................................................21
5.1 Settings Menu .................................................................................................................21
5.2 Configuration of the WLAN Adapter .........................................................................22
5.2.1 Description of Operating Modes.............................................................................22
5.2.2 WLAN Mode................................................................................................................22
5.2.3 Hotspot Mode .............................................................................................................23
5.2.4 Offline Mode ...............................................................................................................24
5.3 Software Update.............................................................................................................24
5.4 Maintenance ....................................................................................................................25
5.5 Language Settings...........................................................................................................25
5.6 Displaying system information ....................................................................................26
6Carrying Out Measurements...........................................................................................27
6.1 Preliminary Remarks ......................................................................................................27
6.2 Spectrometric Measurements with the fluidlab R-300 ..........................................27
6.2.1 General..........................................................................................................................27
6.2.2 Using the Cuvettes .....................................................................................................27
6.2.3 Extinction......................................................................................................................28
6.2.3.1 General......................................................................................................................28
6.2.3.2 Determining the Extinction of Liquids................................................................28
6.2.4 Kinetics..........................................................................................................................32
6.2.4.1 General......................................................................................................................32
6.2.4.2 Determining the Reaction Kinetics of a Liquid Sample ...................................33
6.2.5 Calibration Curve ........................................................................................................36
6.2.5.1 Creating calibration curves....................................................................................36
6.2.5.2 Sample Quantification with Created Calibration Curve..................................40
6.3 Microscopic Measurements with the fluidlab R-300..............................................42

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6.3.1 How the Holographic Microscope Works............................................................. 42
6.3.2 Preparation of the acella Sample Carrier ............................................................... 42
6.3.3 Cell Count..................................................................................................................... 44
6.3.3.1 Automatic Cell Counting ....................................................................................... 44
6.3.4 Viability......................................................................................................................... 48
6.3.4.1 Automatic Viability Measurement....................................................................... 48
6.4 History.............................................................................................................................. 51
6.5 Quality Assurance .......................................................................................................... 52
6.5.1 Sensor Status............................................................................................................... 52
6.5.2 Refresh Sensor Data .................................................................................................. 53
7anvajo datalab .................................................................................................................... 55
7.1 Connection to the fluidlab R-300 ............................................................................... 55
7.1.1 Connection via WLAN............................................................................................... 55
7.1.2 Connection via Hotspot ............................................................................................ 55
7.2 Data Export ..................................................................................................................... 56
7.3 Updates via datalab........................................................................................................ 56
8Operating and Maintenance Instructions .................................................................... 57
8.1 Prohibitions on Use........................................................................................................ 57
8.2 Description of Use ......................................................................................................... 57
8.2.1 User Groups................................................................................................................. 57
8.2.2 User Expertise ............................................................................................................. 57
8.2.3 Service Life................................................................................................................... 57
8.3 Usage Location ............................................................................................................... 58
8.4 Warranty Provisions ...................................................................................................... 58
8.5 Cleaning............................................................................................................................ 59
8.5.1 General.......................................................................................................................... 59
8.5.2 External Cleaning........................................................................................................ 60
8.5.3 Cleaning the Sensor and Measuring Chamber...................................................... 60
8.6 Maintenance Activities.................................................................................................. 62
8.7 Service/Support.............................................................................................................. 62
8.8 Transportation/Storage ................................................................................................ 62
8.9 Decommissioning ........................................................................................................... 63
8.10 Disposal............................................................................................................................ 63
9Technical Specifications................................................................................................... 65
9.1 fluidlab R-300 specifications........................................................................................ 65
9.2 Accessories...................................................................................................................... 67
10 Declaration of Conformity .............................................................................................. 69

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2 Safety Instructions/Hazard Analysis
2.1 Symbols Used in the User Manual and on the Device
Symbol
Meaning
Warning! Please observe the warnings and precautions in this
manual to enable you to use the product safely.
Follow the instructions for use.
Temperature restrictions/permissible temperature
range/temperature limits for storage and transport.
Serial number
Manufacturer
USB port
Standby switch
CE mark
Waste symbol; the black bar indicates a key date regulation
(cf. 2012/19/EU)
Direct current
Biological Risks
Nameplate with device-specific information, such as serial
number, manufacturer and system name (a dummy serial
number is shown in the illustration)

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2.2 General Safety Instructions
Warning!
The system is designed for life science research only. Not for use in diagnostic
procedures. The safety regulations that apply in the relevant areas of application must
be observed.
Warning!
Before using the system, please read the user manual and observe all safety
instructions. Each user must be made aware of this user manual before using the
system; the user manual must be kept close at hand at all times. The user manual is not,
however, able to represent all possible dangers.
Each user is responsible for complying with the safety and health regulations and
determining and observing any restrictions before using the device.
These restrictions include avoiding the use of hazardous substances. Please notify
anvajo GmbH if any malfunctions, faults or damage to the system are observed during
operation.
Warning!
To avoid the risk of electric shock, the system must be operated in its original condition,
closed on all sides.
The system must be operated within the nominal operating range specified in Chapter
9.1.
Warning!
Use only original parts, or parts recommended by the manufacturer, to avoid damage
to the system and to ensure it functions safely.
Warning!
If the device is opened, the manufacturer shall not be liable for the device or for any
damage caused thereby. The warranty provisions are set out in Chapter 8.4.
The system must be protected from damp and moisture (for protection rating, see
Chapter 9.1). Care must be taken not to spill any liquid over the system or insert any
objects through openings.
You must not repair the system yourself. In the event of a defect, please inform the
device manufacturer’s service department (Chapter 8.7).
Do not use sharp or pointed objects to operate the touch screen.

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We recommend the use of gloves when working with the device in order to avoid
contamination by direct contact with any infectious samples or sample residues being
used.
To ensure the system is functioning correctly, all quality control procedures and
cleaning specified in the accompanying documents must be carried out.
In the event of any changes to the system, it is necessary to verify compliance with the
requirements of standard EN 61010-1.
Warning!
An LED is used during fluid measurements / reference measurements. Avoid looking
directly into the active LED, as this may damage the retina.
Warning!
If the device is used in a manner not specified by anvajo GmbH, the protection
supported by the device may be impaired.
2.3 Electromagnetic Considerations
The system is intended for use in an industrial electromagnetic environment.
Do not operate the system in the immediate vicinity of other devices or with other
devices in stacks, as this could result in incorrect functioning.
The use of accessories or cables other than those specified by the manufacturer may
lead to increased electromagnetic interference or reduced electromagnetic immunity
of the system and could result in incorrect functioning.
Portable RF communication devices (mobile phones, radios) should not be used at a
distance of less than 30 cm from the system. Failure to observe this instruction may
lead to a reduction in performance.
The system has been tested in accordance with EN 61326-1 and ETSI EN 301 489-17.
The system is not known to cause any interference. Despite testing, a deterioration in
EMC behaviour (transmission and reception) may occur, e.g. as a result of the aging or
failure of assemblies. In such cases, the system must be checked at the manufacturer’s
premises.
Unforeseen interference from the system may occur if the level of interference
exceeds the levels required by IEC 61326-1 and ETSI EN 301 489-17. This may occur
as a result of the choice of installation site, e.g. amplification of existing sources of
interference. In this event, the operator should position the system in such a way as to
minimise any interference.
The system has been tested in accordance with EN 62311 and EN 62479. The system
is not known to expose the user to any electromagnetic radiation above the level of
the basic restrictions for general public exposure specified by these standards.

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2.4 Lithium Polymer Battery
The system is equipped with a lithium polymer battery. Improper use may lead to a
reduction in battery life. To avoid irreversible capacity losses, please observe the
following principles:
The optimum temperature range is 10°C to 25°C.
Warning!
Only charge the battery in the specified nominal operating range (see Chapter 9)!
Failure to observe this instruction may result in permanent damage to the battery.
Avoid fully discharging the battery. A lower charge cycle depth (starting the charging
process before a 100% discharge) will increase the battery’s service life.
Note: Lithium batteries do not exhibit a memory effect.
If storage should be necessary, aim to achieve the optimum storage with a remaining
battery capacity of 30% to 40%. Temperatures should be below room temperature
(approx. 20°C). To avoid excessive discharge, the battery should be charged to at least
40% once every six months.
The battery should only be replaced by the manufacturer (Chapter 8.7).
Warning!
Return the battery in a discharged state (see Chapter 8.9).
Warning!
Many chargers consume power while they are plugged in at the socket –even if they
are not charging. Disconnect the charger from the mains after charging.
2.5 Radio Communications
The system has been tested in accordance with ETSI EN 300 328.
The user should only connect the device to trusted wireless networks (WLAN) that are
protected from unauthorised access over the Internet, through the use of firewalls.
Always make sure that the data is shared and received only with devices that are
trusted and properly secured.
Obstacles (e.g. walls, fences, grates) located between the device and the devices to
which it is connected (e.g. WLAN routers, computers with WLAN) may reduce the
maximum range of the WLAN connection. Furthermore, in such cases a decrease in
the data transfer rate may be expected.
anvajo GmbH will not be responsible for the loss, interception or misuse of data sent
or received when using the device's wireless communication methods.

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2.6 Protection Against Ultraviolet Radiation (UV)
The measuring system is equipped with UV LEDs. The whole system is a continuous
wave lamp system, which is classified in the exempt group in accordance with EN
62471:2009. When the system is used as intended, these light sources pose no danger
to the user.
Warning!
An LED is used during fluid measurements / reference measurements. Never look
directly into the light sources while the system is in operation, as this may damage the
retina.
Warning!
The device shall only be used with the accessories specified by anvajo GmbH.
As far as possible, the slider should be closed during the measuring process.
The device must be switched off while the measuring chamber shaft is being cleaned.
The biological effects arising from UV LEDs depend on the emitted wavelength, the
exposure time and the intensity. Potential damage may include: thermal damage,
blurring or blind spots on the retina.
In rare cases, LED radiation may also cause thermal damage to the skin (sunburn).
If light escapes as a result of damage to the housing, immediately switch off the device
and send it in for repair.

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3 Introduction
3.1 A Brief Description and Intended Purpose
The fluidlab R-300 is a compact and versatile fluid analysis device which has been
developed for research applications, with which a large variety of liquids can be
analysed quickly, easily and inexpensively –both spectrometrically and
microscopically. The fluidlab R-300 is operated via an intuitive touch screen.
Fluid samples must be inserted into the fluidlab R-300 in the sample carriers provided.
Using the various adapters that are supplied and which can also be ordered from
anvajo, the fluidlab R-300 can be made compatible with different sample carrier
formats (acella for microscopic measurements, cuvette for spectrometric
measurements).
Overview of the most important performance parameters:
• Spectrometer for measuring extinction (absorption and scattered light), for
creating calibration curves and kinetics plots
• Microscope for cell counts and viability measurements
• Touch screen (for performance data see Chapter 9.1)
• Mains or battery operation possible (for performance data see Chapter 9.1)
• WLAN or USB connection (WLAN as client or hotspot, USB for charging)
• Display of measured value, evaluation via touch screen
The main benefits of the fluidlab R-300 include:
• Quick identification and display of quantitative test results
• Measurements for different sample carrier formats with a single system
• Portable, battery-operated system
• Simple, user-friendly operation
anvajo microscope
Optics
Holographic microscope
Sample volume required
4 –20 µL
Concentration range of the sample
1x104–1x107cells / mL
Cell size limits
3 - 80 µm (cell count)
8 - 80 µm (viability)
Field of view
5.29 mm²
anvajo spectrometer
Photometric Measuring Range
0 - 2.5
Spectral bandwidth
~2nm
Wavelength range
375 - 700 nm

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3.2 Overview
The fluidlab R-300 contains a miniaturised high-performance spectrometer that works
in the UV/VIS wavelength range from 375 nm to 700 nm. This allows measurements
of absorption and scattered light to be carried out on a liquid sample inserted in a
sample carrier.
The integrated next-generation microscope captures images of particles in an
extremely large field of view (FOV 2.3 mm x 2.3 mm) and guarantees high statistical
reliability for every test result. The hologram is then processed by anvajo’s intelligent,
mathematical reconstruction software, which delivers high-resolution images.
Robust and fast image signal processing and data-parallel operations, combined with a
high level of computing power, make efficient analysis of microscopic images possible
directly in the fluidlab R-300 (local processing, no cloud service required). In addition,
machine learning methods, such as deep neural networks, guarantee accurate counting
and classification of the optical outputs with immediate quantitative results.

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3.3 Product Set Components
Figure 1: Product set components.
No.
Name
Description
1
fluidlab R-300
Measuring device with inserted sample carrier
adapter 01: acella
2
Technical
documentation
User manual
Quick Start Guide
3
USB battery charger
See Chapter 9.2
4
USB-C cable
See Chapter 9.2
5
Cleaning swabs
See Chapter 8.5.3
6a
Adapter starter set
Sample carrier adapter 02:
Cuvette for scattered light measurements
6b
Adapter starter set
Sample carrier adapter 03:
Cuvette for absorption measurements
7
Adapter key
Tool for changing the adapter
Note: Please check that the product set is complete, based on the delivery note. Only
products and accessories that are completely free of defects should be put into
operation. Damage must be reported immediately to anvajo GmbH or its
representatives.
Note: Please note the full part number (see chapter 9.2) as specified on the delivery note
for your orders!
3
4
1
6
7
5
2

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3.4 Description of Device
Figure 2: Detailed description of device.
No.
Name
Description
1
USB-C socket
Port for the charging cable
2
Power on/off button
Switches the fluidlab R-300 on or off
3
Touch screen
Interface for interaction with the device
4
Slider
Protects the measuring chamber (shown open in the
illustration)
5
Sample carrier adapter
Sample carrier-specific adapter (exchangeable)
6
Slot for sample carrier
For inserting the sample carrier (opening corresponds
to the space between the adapter and the measuring
chamber).
7
Measuring chamber
Holder for sample carrier adapter
1
2
3
4
5
6
7

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4 Setup and Use
Note: If used as a portable device, the fluidlab R-300 must be held in one hand and
operated with the other free hand. If it is being used as a desktop device or when it
is being charged, it should be placed on a stable, level, secure surface to prevent it
from falling over and being damaged.
Warning!
During the analysis, the device should be kept still. It is recommended that the device
should be operated in a stationary manner on a stable surface.
4.1 Initial Setup
Note: On delivery, the fluidlab R-300 is in shipping mode. In order to start up your
fluidlab R-300 for the first time, please connect it to the supplied USB charger using
the supplied USB cable before switching it on for the first time.
1. Remove the protective film from the device’s display by taking hold of the
small tab on the top right-hand edge of the film (near the power on/off button)
and carefully pulling it off downwards over the display.
2. Connect the device to the supplied USB charger, the USB cable and a mains
socket –the same procedure as when charging the battery (see Chapter 4.1).
3. The power on/off button (see Figure 2-2) lights up briefly. The device is no
longer in shipping mode and can be switched on (see Chapter 4.2).
4.1 Charging the Built-in Battery
Warning!
Throughout the charging period, the fluidlab R-300 must be placed on a stable, level,
secure, fire-resistant surface. The charging process may cause the bottom of the device
to heat up.
Warning!
The device may only be charged and operated using the supplied power supply unit
described in the specifications (Chapter 9.2). If the power supply unit fails, a
replacement power supply unit should be requested from the manufacturer.
Warning!
Only charge the battery in the specified nominal operating range (see Chapter 9.1)!
Failure to observe this instruction may result in permanent damage to the battery.
1. Place the fluidlab R-300 on a stable, level, secure, fire-resistant surface.
2. Plug the supplied USB charger (Figure 1–3) into a mains socket.
3. Plug the larger of the two connectors on the USB cable (Figure 1–6) into the
USB port on the USB charger.

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4. Plug the other end of the USB cable into the USB-C socket on the fluidlab R-
300 (Figure 2-1).
5. The charging process is active when the power on/off button (Figure 2-2) on
the fluidlab R-300 lights up at regular intervals.
6. The battery is fully charged when the power on/off button stops lighting up at
regular intervals or when the battery icon on the display is full.
7. Disconnect the USB charger from the device when the battery is fully charged.
First unplug the connector on the USB cable from the device before
unplugging the power supply unit from the mains socket.
Note: The fluidlab R-300 can be switched on and used for measurements during the
charging process. It is not necessary to wait until the battery is fully charged before
switching the device on.
4.2 Switching On the Device
Note: In order to be able to switch on the fluidlab R-300, either the device battery must
be charged (partially or fully) or the USB charger must be connected to the device
with the USB cable (see Chapter 4.1).
1. Switch the unit on by pressing and holding down the power on/off button
(Figure 2-2) for approx. one second and then releasing it. The device is
switched on as soon as the power on/off button lights up.
2. Wait until the system has started. This process takes approx. 40 seconds.
Start-up is complete when the main menu appears on the display. The device
is now ready for operation.
4.3 Switching Off the Device
Note: There are several possible ways of switching off the fluidlab R-300. For fault-free
operation of the fluidlab R-300, you should use a “normal shutdown” whenever
possible. If a “normal shutdown” is not possible (e.g. as a result of a non-responsive
system), please select “error shutdown”.
Normal shutdown (recommended):
1. Press and hold down the power on/off button (Figure 2-2) on the fluidlab R-
300 for approx. one second until a selection menu (“Shut down the device –
Yes/No”) appears on the display. Then immediately release the power on/off
button.
2. Tap “Yes” to switch off the device. If you tap “No”, the process is cancelled,
and the device will remain switched on.
Error shutdown:
Warning!
Only perform this procedure if a “normal shutdown” (see above) is not possible.

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1. Press and hold down the power on/off button (Figure 2-2) on the fluidlab R-
300 for approx. 8 seconds until the LED starts flashing at a higher frequency.
Then immediately release the power on/off button.
2. This will switch the device off immediately. Under certain circumstances, this
may result in data loss of the most recent measurements.
4.4 Exiting Energy-saving Mode
Note: The fluidlab R-300 has a built-in energy-saving function which, after a predefined
period of inactivity, first reduces the brightness of the display and then switches off
the display backlight (energy-saving mode). Apart from the display of the graphical
user interface, the device is fully functional in this mode.
Energy-saving mode:
• Automatically activated after approx. 2 minutes of non-use
• Display is dimmed after 2 minutes and then switched off
• Power on/off button (Figure 2-2) lights up cyclically (“pulsating”)
To “wake up” the fluidlab R-300 from power-saving mode and return it to the active
state, proceed as follows:
1. Tap on the touch screen.
2. As soon as the display surface lights up on the touch screen, the system has
left energy-saving mode and is back in operating mode.

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5 Setting up the System
5.1 Settings Menu
The settings menu can be called up at any point on the fluidlab R-300 by tapping the
menu icon ( ). The fluidlab R-300 is supplied pre-configured, which means that it is
not absolutely necessary to configure the settings.
Figure 3: Settings menu of the fluidlab R-300.
You can use the following functions from the Settings menu:
No.
Name
Function
1
WLAN button
Display WLAN settings (see Chapter 5.2)
2
WLAN switch
Enable/disable WLAN (see Chapter 5.2)
3
Hotspot button
Display hotspot menu (see Chapter 5.2)
4
Hotspot switch
Activate/deactivate hotspot (see Chapter 5.2)
5
Update button
Display Update menu (see Chapter 5.3)
6
Update indicator
Shows available software updates (see Chapter 5.3)
7
Maintenance
Sensor status and “Refresh Sensor” (see Chapter 6.5)
8
Language
Display language settings (see Chapter 5.5)
9
Time / Date
Display time / date settings
10
System
Display system information (see Chapter 5.6)
2
1
4
3
5
6
7
8
9
10