Knauer K-7400S User manual

Freezing Point Osmometer
User Manual
K-7400S Semi-Micro Osmometer

For your own safety, read the manual and always observe the warnings and
safety information on the device and in the manual
Manuel en français: Si jamais vous préfériez un manuel en français pour ce produit, veuillez
vous contacter le support technique (Technical Support) par email ou par
fax avec le no. de série. Merci beaucoup.
Technical Support: Phone: [phone] (9-17h, Central European Time)
Fax: [phone]
E-Mail: [email protected]
Languages: German, English
Publisher: KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
D-14163 Berlin
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Version information: Article number: V3700
Version number: 2.0
Release date: 2017/07/12
Translation of the original edition
The information in this document is subject
to change without prior notice.
For latest version of the manual, check our website:
http://www.knauer.net/knowledge/user-manu-
als.html
Copyright: © KNAUER Wissenschaftliche Geräte GmbH 2017
All rights reserved.

K-7400S user manual V3700
Table of contents
Table of contents
Product information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Front view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Rear view . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Safety for users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Signal words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Symbols and signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Preparations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Work location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Unpacking the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Start-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Switch-on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Connecting the measuring head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Stand-by position of measuring head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Measurement principle and process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Switch-on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Menu guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Preparing a measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Measurement preparation process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Pre-calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Measurement calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Measuring sample solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Performing a sample measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Important operating notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Functionality tests. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
System messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Self-freezing of the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Non-freezing of the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Using the EuroOsmo 7400 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Hardware installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Main screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Run Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Sample list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

K-7400S user manual V3700
Table of contents
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Working parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Report & Graph . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Search function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Maintenance and care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Cleaning the stirring wire and the thermistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Function checks of cooling capacity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Reference thermometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Main features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Repeat orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Legal information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Transport damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Warranty conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
KNAUER Software License Agreement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

K-7400S user manual V3700
1 Product information
Product information
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the
device could fail.
Views
Front view
Keypad
Legend
1Measuring head
2LCD display
3Keypad
Fig. 1 Front view
Buttons Function
START/STOP Starts a measurement or calibration run. If this button
has no specific function on the screen, it causes to return
on the main screen.
With this button, every running measurement can be
interrupted.
VIB Activates the stirring wire. During RUN and CALIBRATE,
the button is without any function.
Arrows Cursor navigation buttons for parameter or value selec-
tion
1
2
3

K-7400S user manual V3700
Scope of delivery 2
Rear view
Scope of delivery
Note: Only use original parts and accessories made by KNAUER or a com-
pany authorized by KNAUER.
Freezing Point Osmometer K-7400S
Power cable
User manual (German/English)
Installation Qualification document ("IQ", English)
RS-232 connection cable
Measuring head
Pack of sample tubes
400 mOsmol/kg calibration solution
850 mOsmol/kg calibration solution
Lint-free cleaning tissue
Safety for users
Professional group The user manual addresses persons who are qualified as chemical labora-
tory technicians or have completed comparable vocational training.
The following knowledge is required:
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
Safety equipment When working with the device, take measures according to lab regulations
and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken
into account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the
user manual
Legend
1RS-232 connector
2Measuring head
connector
3Ventilator
4Power switch
5Power connector
Fig. 2 Rear view
5
4
3
2
1

K-7400S user manual V3700
3Safetyforusers
National and international regulations pertaining to laboratory work
Original spare parts and tools made or recommended by KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Power cable: Defective power cables are not to be used to connect the
device and the power supply system.
Power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
Power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
Where is use of the
device prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Technical
Support of KNAUER.
Secure decommission-
ing
At any time, take the device completely out of operation by either switch-
ing off the power switch or by pulling the power plug.
Opening the device The device may be opened by the KNAUER Technical Support or any com-
pany authorized by KNAUER only.
Signal words
Possible dangers related to the device are divided into personal and mate-
rial damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal
of a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamina-
tion must be collected separately and disposed of properly.
Decontamination
report
Devices without a completed Decontamination Report will not be
repaired. If you would like to return a device to KNAUER, make sure to
enclose a completed Decontamination Report with the device: http://
www.knauer.net/en/downloads/service.html

K-7400S user manual V3700
Symbols and signs 4
Symbols and signs
The following symbols and signs can be found on the device or in the user
manual:
Installation
This chapter describes all preparatory steps prior to start-up.
Preparations
Note: To ensure structural stability, the device is not intended to be
stacked on or below other devices.
Work location
The intended use be ensured only if the requirements for ambient condi-
tions of the operating environment are met. You will find the ambient con-
ditions in the chapter "Technical data" (see page 25).
General Purpose Position the device on a level surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts such as air con-
ditioning systems.
Do not set up the device near to other machines that cause floor vibra-
tions.
Keep the the devices away from high frequency sources. High frequen-
cies may compromise measurement values.
Space requirements At least 5 cm, if there is another device on one side.
At least 10 cm, if there are devices set up on both sides.
At least 15 cm to the cooler fan on the rear.
Ambient temperature The device should not be exposed to major temperature fluctuations
during measurement.
Symbol Meaning
Warning Signs Electric shock hazard
Electrostatic discharge hazard, damages to system,
device, or components can occur.
Biohazard
CE Mark A device or system marked with CE fulfills the product
specific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Note Hints provide useful tips or information worth knowing.

K-7400S user manual V3700
5 Installation
Device defect
The device overheats at exposure to sunlight and insufficient air circula-
tion. Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-
culation.
Power supply
Use only the enclosed power cable to connect the device to the mains to
make sure that the specifications stated in Technical Data are met. But
check beforehand to use power cables admitted for use in your country.
Replace defective power cables only with accessories from KNAUER.
Detachable power cables must not be replaced with different cable types.
The maximum power input is 70 watts.
Electronic defect
Electronic hazard when using an identically constructed power adapter
from another manufacturer.
Only use original parts and accessories made by KNAUER or a company
authorized by KNAUER.
Note: The nominal capacity of the connected devices must be maximum
50 % of the power supply to account for larger inrush currents when
switching on the modules.
Prerequisites The electrical power supply at the installation site must be connected
directly to the nearest main power line.
The power must be free from ripple, residual current, voltage peaks and
electromagnetic interference.
The connectors for the mains voltage are grounded accordingly.
The device receives sufficient power with reserve capacity.
Power plug The device is intended for use with AC power networks of 100 – 240 V.
Make sure that the power plug on the rear of the device is always acces-
sible, so that the device can be disconnected from the power supply.
To disconnect the device from the main power, disconnect the power
plug.
The osmometer needs a three-core power plug.
Electric shock
Make sure that the power cable is not exposed to high stresses.
The power cable should not be bent or clamped.
Make sure that the power cable does not come in contact with liquids
and heat sources.
Unpacking the device
At the factory, all KNAUER devices are carefully packed for safe transport.
After unpacking, check all device parts and accessories for signs of dam-
age during transport and if necessary assert claims for damages immedi-
ately to the transport company.
The complete listing of consumable and spare parts can be found in sec-
tion "Accessories and spare parts" on page 26.
Prerequisite Check packaging for damage caused during transportation. If necessary,
put forward any claim for damages to the carrier.
Tools Utility knife

K-7400S user manual V3700
Installation 6
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Process 1. Set up the package in such a way that you can read the label. Using the
utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessory kit and the manual.
3. Open the accessory kit and check the scope of delivery. In case any
parts are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging and place it on
its feet.
5. Check the device for signs of damage that occurred during transport. In
case you notice any damage, contact the Technical Support.
6. Place the device in its site of operation and remove protective foil.
Next steps Store packaging and keep the included packing list for repeat orders.
Start-up
Note: The device is not suitable for constant operation because it would
reduce the lifespan of the Peltier elements.
Risk of Infection
Risk of infection by injuries caused by a contaminated stirring wire.
Wear safety gloves when handling the measuring head.
Skin Injury
The cooling compartment reaches temperatures up to -30 °C during the
running measurement. Contact with unprotected hands may lead to skin
injuries.
Avoid direct contact with the cooling compartment.
For cleaning or drying, use cotton swabs or a lint-free tissue.
Switch-on
For information and safety instructions for power supply, see page 5.
Electric shock
High voltages build up inside the device, the housing serves as a protec-
tive cover.
Never put the device into operation when the housing is open.
First close the housing, then plug in the power plug and switch the
device on.
Electronic defect
Damage to the electronics if the device is turned on while connecting or
interrupting the power connection.
Switch off the device beforehand.
Prerequisites The device is switched off. The power cable is detached from the power
supply.
Procedure 1. Attach the power cable to the power connector on the rear of the
device.
2. Switch the device on by using the "ON/OFF" switch.
Next Steps Bring the device into operation. To put the device out of operation, switch
off the device and detach the power plug from the power supply.