Applied Biosystems Veriti User manual

Applied Biosystems
Veriti™Thermal Cycler
User Guide

Applied Biosystems
Veriti™Thermal Cycler
User Guide

© Copyright 2008, 2010 Applied Biosystems. All rights reserved.
General Laboratory Use.
Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this
document.
APPLIED BIOSYSTEMS DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT, EXPRESSED OR IMPLIED, IN-
CLUDING BUT NOT LIMITED TO THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. IN NO EVENT SHALL
APPLIED BIOSYSTEMS BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER
BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
NOTICE TO PURCHASER:
The Veriti™Thermal Cycler is covered by US patent claims and corresponding claims in their non-US counterparts. No right is conveyed expressly, by
implication, or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods such as 5’ nuclease methods. Further in-
formation on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City,
California 94404, USA.
TRADEMARKS:
Applera, Applied Biosystems, AB (Design), ABI PRISM, BigDye, and GeneAmp, are registered trademarks and MicroAmp, VeriFlex, and Veriti are
trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries.
AmpliCycle, AmpliTaq, and AmpliTaq Gold is a registered trademark of Roche Molecular Systems, Inc.
All other trademarks are the sole property of their respective owners.
Part Number 4375799 Rev. E
06/2010

Contents Front Matter
iiiVeriti™ Thermal Cycler User Guide
Preface
How to Use This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
How to Obtain More Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
How to Obtain Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii
Safety and EMC Compliance Information
Safety Conventions Used in This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Symbols on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Safety Labels on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiv
General Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvi
Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Physical Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety and Electromagnetic Compatibility (EMC) Standards . . . . . . . . . . . . . . . xvii
Chapter 1 System Overview
Introducing the Veriti™Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Using the Touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Menu Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
About Run Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
About Folders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-15
Chapter 2 Getting Started
Installing the Veriti™Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Before You Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Unpacking the Veriti™System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Setting Up the Veriti™System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Connecting to a Network Printer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Connecting Together Multiple Instruments . . . . . . . . . . . . . . . . . . . . . . . . . 2-5

iv Veriti™ Thermal Cycler User Guide
Powering On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Setting Up the Veriti™Thermal Cycler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Recommended Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7
Setting Up Instrument Security and User Accounts . . . . . . . . . . . . . . . . . . . 2-9
Adding Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
Setting Other Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-14
Chapter 3 Performing a Run
Selecting Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Disposables for 96-Well Veriti™Thermal Cyclers . . . . . . . . . . . . . . . . . . . . . 3-2
Disposables for the 384-Well Veriti™Thermal Cycler . . . . . . . . . . . . . . . . . . 3-4
Disposables for the 60-Well Veriti™Thermal Cycler . . . . . . . . . . . . . . . . . . . 3-4
Loading Samples into the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Loading Samples in Veriti™96-Well Thermal Cyclers . . . . . . . . . . . . . . . . . . 3-5
Loading Samples in the Veriti™384-Well Thermal Cycler . . . . . . . . . . . . . . . 3-6
Loading Samples in the Veriti™60-Well Thermal Cycler . . . . . . . . . . . . . . . . 3-7
Creating a New Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Editing Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Performing a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
Monitoring a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
Viewing and Printing the Run Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Removing the Samples from the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-25
Chapter 4 Troubleshooting
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Returning an Instrument for Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Chapter 5 Utilities
Using GeneAmp®PCR System 9700 Run Methods . . . . . . . . . . . . . . . . . . . . . . 5-2
Upgrading System Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
Calculating Melting Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-7
Viewing Instrument Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Restoring the Factory Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
Running the Cycle Performance Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
Tests Using the Temperature Verification Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Running the Heated Cover Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
Running the Temperature Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-12
Running the Temperature Nonuniformity Test . . . . . . . . . . . . . . . . . . . . . . 5-13
TVK Recalibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-13

vVeriti™ Thermal Cycler User Guide
Chapter 6 Maintenance
Scheduled Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
As-Needed Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
Cleaning the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-2
Replacing Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-4
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-6
Appendix A Instrument Specifications
System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
Printer Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3
Appendix B Disposables
Disposables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-2
Other Supplies for Sample Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-4
Appendix C Predefined Run Methods
Predefined Run Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
AmpliCycle®Seq Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-2
AmpliTaq Gold®DNA Polymerase Run Method . . . . . . . . . . . . . . . . . . . . . . C-3
AmpliTaq Gold®Fast PCR Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-3
BigDye®Kit Fast Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-4
BigDye®Kit Standard Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-4
Fast PCR Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-5
General PCR Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-5
LMS2 Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6
Time Release Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6
Touchdown PCR Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-7
XL PCR Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-7
Index

vi Veriti™ Thermal Cycler User Guide

viiVeriti™ Thermal Cycler User Guide
Preface
How to Use This Guide
Purpose of This
Guide
The Applied Biosystems Veriti ™ User Guide provides information about installing,
using and maintaining the Applied Biosystems Veriti Thermal Cycler.
Audience This guide is intended for novice and experienced Veriti Thermal Cycler users who
use the instrument to perform PCR.
Assumptions This guide assumes you have knowledge of basic laboratory procedures and PCR
chemistry.
Te x t C o n v e nt i o n s This guide uses the following conventions:
•Bold text indicates user action. For example:
Ty p e 0, then press Enter for each of the remaining fields.
•Italic text indicates new or important words and is also used for emphasis.
For example:
Before analyzing, always prepare fresh matrix.
• A right arrow symbol () separates successive commands you select from a
drop-down or shortcut menu. For example:
Select FileOpenSpot Set.
Right-click the sample row, then select View FilterView All Runs.
User Attention
Words
Two user attention words appear in Applied Biosystems user documentation. Each
word implies a particular level of observation or action as described below:
Note: – Provides information that may be of interest or help but is not critical to the
use of the product.
IMPORTANT! – Provides information that is necessary for proper instrument
operation, accurate chemistry kit use, or safe use of a chemical.
Examples of the user attention words appear below:
Note: The Calibrate function is also available in the Control Console.
IMPORTANT! If your instrument is so configured, you need a valid user ID and
password to log in.
Safety Alert
Words
Safety alert words also appear in user documentation. For more information, see
“Safety Alert Words” on page xii.

viii Veriti™ Thermal Cycler User Guide
Preface
How to Obtain More Information
Related
Documentation
The following document is shipped with the system:
Applied Biosystems Veriti™Thermal Cycler Quick Reference Card – Provides a
summary of information about the thermal cycler, its installation, and accessories. It
is designed to help you quickly learn to use the Veriti Thermal Cycler.
A portable document format (PDF) version of this guide is available on the Ve r i t i ™
Thermal Cycler Documentation CD.
Note: To open the user documentation on the Ve r i t i ™Thermal Cycler
Documentation CD, use the Adobe®Reader®software available from
www.adobe.com.
Note: For additional documentation, see “How to Obtain Support” on page viii.
Obtaining
Information from
the Help System
The Veriti Thermal Cycler has a Help system that describes how to use each feature
of the user interface. Access the Help system by touching (Help) at the bottom
right corner of the instrument touchscreen.
Send Us Your
Comments
Applied Biosystems welcomes your comments and suggestions for improving its
user documents. You can e-mail your comments to:
[e-mail]
IMPORTANT! The e-mail address above is for submitting comments and suggestions
relating only to documentation. To order documents, download PDF files, or for help
with a technical question, go to http://www.appliedbiosystems.com, then click the
link for Support. (See “How to Obtain Support” below).
How to Obtain Support
Support
Information
For the latest services and support information for all locations, go to
http://www.appliedbiosystems.com, then click the link for Support.
At the Support page, you can:
• Search through frequently asked questions (FAQs)
• Submit a question directly to Technical Support
• Order Applied Biosystems user documents, MSDSs, certificates of analysis,
and other related documents
• Download PDF documents
• Obtain information about customer training
• Download software updates and patches
• Contact technical services by clicking the Care Center link.
In addition, the Support page provides access to worldwide telephone and fax
numbers to contact Applied Biosystems Technical Support and Sales facilities.

ixVeriti™ Thermal Cycler User Guide
Preface
TVK Support To send the temperature verification kit equipment for recalibration:
1. Use the address or Fax information below to request a quote from Alpha
Technics for a recalibration order:
• Mail: Alpha Technics
17151 Gillette Avenue
Irvine, Ca. 92614-5602
• Fax: [phone]
2. Ensure that the probe assembly has been decontaminated (see
“Decontaminating the Probe Assembly” on page 5-13).
3. Pack the digital thermometer and the probe assembly in the black case.
4. Create a shipping package with:
• Black case containing the digital thermometer and probe assembly
• Completed copy of the Certificate of Instrument Decontamination
• Payment for shipping/handling in one of the following forms:
– Purchase order
– Company letterhead with the words “verbal purchase order”
– Visa/MasterCard credit card information
• Your address and contact information:
– Billing address
– Return shipping address
– Name and phone number of a contact (person most familiar with the
thermometer)
Note: If payment is not included with the package, Alpha Technics bills you for
$150.00 plus a 10% administrative fee. The total for the purchase order is then
$165.00.
5. Send the package freight prepaid to Alpha Technics at the address in step 1.

xVeriti™ Thermal Cycler User Guide
Preface

xiVeriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
This section covers:
Safety Conventions Used in This Document. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Symbols on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Safety Labels on Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiv
General Instrument Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvi
Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Physical Hazard Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety and Electromagnetic Compatibility (EMC) Standards . . . . . . . . . . . . . . . xvii

xii Veriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
Safety Conventions Used in This Document
Safety Alert
Words
Four safety alert words appear in Applied Biosystems user documentation at points
in the document where you need to be aware of relevant hazards. Each alert
word—IMPORTANT, CAUTION, WARNING, DANGER—implies a particular
level of observation or action, as defined below.
Definitions
IMPORTANT! – Indicates information that is necessary for proper instrument
operation, accurate chemistry kit use, or safe use of a chemical.
– Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
– Indicates a potentially hazardous situation that, if not avoided,
could result in death or serious injury.
– Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be limited to the
most extreme situations.
Except for IMPORTANTs, each safety alert word in an Applied Biosystems
document appears with an open triangle figure that contains a hazard symbol. These
hazard symbols are identical to the hazard symbols that are affixed to Applied
Biosystems instruments (see “Safety Symbols” on page xiii).
Examples
The following examples show the use of safety alert words:
IMPORTANT! You must create a separate sample entry spreadsheet for each 96-well
plate.
The lamp is extremely hot. Do not touch the lamp until it has
cooled to room temperature.
CHEMICAL HAZARD. Formamide. Exposure causes eye,
skin, and respiratory tract irritation. It is a possible developmental and birth defect
hazard. Read the MSDS, and follow the handling instructions. Wear appropriate
protective eyewear, clothing, and gloves.
ELECTRICAL HAZARD. Failure to ground the instrument
properly can lead to an electrical shock. Ground the instrument according to the
provided instructions.

xiiiVeriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
Symbols on Instruments
Electrical
Symbols on
Instruments
The following table describes the electrical symbols that may be displayed on
Applied Biosystems instruments.
Safety Symbols The following table describes the safety symbols that may be displayed on
Applied Biosystems instruments. Each symbol may appear by itself or with text that
explains the relevant hazard (see “Safety Labels on Instruments” on page xiv). These
safety symbols may also appear next to DANGERS, WARNINGS, and CAUTIONS
that occur in the text of this and other product-support documents.
Symbol Description
Indicates the On position of the main power switch.
Indicates the Off position of the main power switch.
Indicates a standby switch by which the instrument is
switched on to the Standby condition. Hazardous
voltage may be present if this switch is on standby.
Indicates the On/Off position of a push-push main
power switch.
Indicates a terminal that may be connected to the signal
ground reference of another instrument. This is not a
protected ground terminal.
Indicates a protective grounding terminal that must be
connected to earth ground before any other electrical
connections are made to the instrument.
Indicates a terminal that can receive or supply alternating
current or voltage.
Indicates a terminal that can receive or supply alternating
or direct current or voltage.
Symbol Description
Indicates that you should consult the manual for further
information and to proceed with appropriate caution.
Indicates the presence of an electrical shock hazard and
to proceed with appropriate caution.

xiv Veriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
Environmental
Symbols on
Instruments
The following symbol applies to all Applied Biosystems electrical and electronic
products placed on the European market after August 13, 2005.
Safety Labels on Instruments
The following CAUTION, WARNING, and DANGER statements may be displayed
on Applied Biosystems instruments in combination with the safety symbols
described in the preceding section.
Indicates the presence of a hot surface or other
high-temperature hazard and to proceed with
appropriate caution.
Indicates the presence of a laser inside the instrument
and to proceed with appropriate caution.
Indicates the presence of moving parts and to proceed
with appropriate caution.
Symbol Description
Symbol Description
Do not dispose of this product as unsorted municipal
waste. Follow local municipal waste ordinances for
proper disposal provisions to reduce the environmental
impact of waste electrical and electronic equipment
(WEEE).
European Union customers:
Call your local Applied Biosystems Customer Service
office for equipment pick-up and recycling. See
http://www.appliedbiosystems.com for a list of
customer service offices in the European Union.
English Francais
CAUTION Hazardous chemicals.
Read the Material Safety Data
Sheets (MSDSs) before handling.
ATTENTION Produits chimiques
dangeureux. Lire les fiches
techniques de sûreté de matériels
avant la manipulation des produits.
CAUTION Hazardous waste. Refer
to MSDS(s) and local regulations
for handling and disposal.
ATTENTION Déchets dangereux.
Lire les fiches techniques de
sûreté de matériels et la régulation
locale associées à la manipulation
et l'élimination des déchets.
WARNING Hot lamp. AVERTISSEMENT Lampe
brûlante.

xvVeriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
General Instrument Safety
PHYSICAL INJURY HAZARD. Using the instrument in a
manner not specified by Applied Biosystems may result in personal injury or damage
to the instrument.
Moving and
Lifting the
Instrument
PHYSICAL INJURY HAZARD. The instrument is to be moved
and positioned only by the personnel or vendor specified in the applicable site
preparation guide. If you decide to lift or move the instrument after it has been
installed, do not attempt to lift or move the instrument without the assistance of
others, the use of appropriate moving equipment, and proper lifting techniques.
Improper lifting can cause painful and permanent back injury. Depending on the
weight, moving or lifting an instrument may require two or more persons.
Operating the
Instrument
Ensure that anyone who operates the instrument has:
• Received instructions in both general safety practices for laboratories and
specific safety practices for the instrument.
• Read and understood all applicable Material Safety Data Sheets (MSDSs). See
“About MSDSs” on page xvi.
Cleaning or
Decontaminating
the Instrument
Before using a cleaning or decontamination method other than
those recommended by the manufacturer, verify with the manufacturer that the
proposed method will not damage the equipment.
WARNING Hot. Replace lamp with
an Applied Biosystems lamp.
AVERTISSEMENT Composants
brûlants. Remplacer la lampe par
une lampe Applied Biosystems.
CAUTION Hot surface. ATTENTION Surface brûlante.
DANGER High voltage. DANGER Haute tension.
WARNING To reduce the chance
of electrical shock, do not remove
covers that require tool access. No
user-serviceable parts are inside.
Refer servicing to
Applied Biosystems qualified
service personnel.
AVERTISSEMENT Pour éviter les
risques d'électrocution, ne pas
retirer les capots dont l'ouverture
nécessite l'utilisation d'outils.
L’instrument ne contient aucune
pièce réparable par l’utilisateur.
Toute intervention doit être
effectuée par le personnel de
service qualifié de
Applied Biosystems.
CAUTION Moving parts. ATTENTION Parties mobiles.
English Francais

xvi Veriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
Chemical Safety
Chemical Hazard
Warning
CHEMICAL HAZARD. Before handling any chemicals, refer
to the Material Safety Data Sheet (MSDS) provided by the manufacturer, and
observe all relevant precautions.
About MSDSs Chemical manufacturers supply current Material Safety Data Sheets (MSDSs) with
shipments of hazardous chemicals to new customers. They also provide MSDSs with
the first shipment of a hazardous chemical to a customer after an MSDS has been
updated. MSDSs provide the safety information you need to store, handle, transport,
and dispose of the chemicals safely.
Each time you receive a new MSDS packaged with a hazardous chemical, be sure to
replace the appropriate MSDS in your files.
Obtain MSDSs
from Applied
Biosystems
The MSDS for any chemical supplied by Applied Biosystems is available to you free
24 hours a day. To obtain MSDSs:
1. Go to www.appliedbiosystems.com, click Support, then click MSDS Search.
2. In the Keyword Search field, enter the chemical name, product name, MSDS
part number, or other information that appears in the MSDS of interest, then
click Search.
3. Find the MSDS of interest, click the link or right-click the MSDS title, then
select any of the following:
•Open – To view the MSDS
•Print Target – To print the MSDS
•Save Target As – To download a PDF version of the MSDS
Note: For the MSDSs of chemicals not distributed by Applied Biosystems,
contact the chemical manufacturer.
Chemical Safety
Guidelines
To minimize the hazards of chemicals:
• Read and understand the Material Safety Data Sheets (MSDSs) provided by the
chemical manufacturer before you store, handle, or work with any chemicals or
hazardous materials. (See “About MSDSs” on page xvi.)
• Minimize contact with chemicals. Wear appropriate personal protective
equipment when handling chemicals (for example, safety glasses, gloves, or
protective clothing). For additional safety guidelines, consult the MSDS.
• Minimize the inhalation of chemicals. Do not leave chemical containers open.
Use only with adequate ventilation (for example, fume hood). For additional
safety guidelines, consult the MSDS.
• Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the
manufacturer’s cleanup procedures as recommended in the MSDS.
• Comply with all local, state/provincial, or national laws and regulations related
to chemical storage, handling, and disposal.

xviiVeriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
Chemical Waste Safety
Chemical Waste
Hazard
HAZARDOUS WASTE. Refer to Material Safety Data Sheets
and local regulations for handling and disposal.
Electrical Safety
ELECTRICAL SHOCK HAZARD. Severe electrical shock can
result from operating the Veriti Thermal Cycler without its instrument panels in
place. Do not remove instrument panels. High-voltage contacts are exposed when
instrument panels are removed from the instrument.
Fuses FIRE HAZARD. Improper fuses or high-voltage supply can
damage the instrument wiring system and cause a fire. Before turning on the
instrument, verify that the fuses are properly installed and that the instrument voltage
matches the power supply in your laboratory.
FIRE HAZARD. For continued protection against the risk of
fire, replace fuses only with fuses of the type and rating specified for the instrument.
Power ELECTRICAL HAZARD. Grounding circuit continuity is vital
for the safe operation of equipment. Never operate equipment with the grounding
conductor disconnected. Use properly configured and approved line cords for the
voltage supply in your facility. Plug the system into a properly grounded receptacle
with adequate current capacity.
Overvoltage
Rating
The Veriti Thermal Cycler has an installation (overvoltage) category of II, and is
classified as portable equipment
Physical Hazard Safety
Moving Parts PHYSICAL INJURY HAZARD. Moving parts can crush and
cut. Keep hands clear of moving parts while operating the instrument. Disconnect
power before servicing the instrument.
Safety and Electromagnetic Compatibility (EMC)
Standards
This section provides information on:
•U.S. and Canadian Safety Standards
•Canadian EMC Standard
•European Safety and EMC Standards
•Australian EMC Standards

xviii Veriti™ Thermal Cycler User Guide
Safety and EMC Compliance Information
U.S. and
Canadian Safety
Standards
The Veriti Thermal Cycler has been tested to and complies with standard:
UL 61010A-1/CAN/CSA C22.2 No. 1010.1-92, “Safety Requirements for Electrical
Equipment for Measurement, Control, and Laboratory Use, Part 1: General
Requirements.”
UL 61010A-2-010, “Particular Requirements for Laboratory Equipment for the
Heating of Materials.”
Canadian EMC
Standard
This instrument has been tested to and complies with ICES-001, Issue 3: “Industrial,
Scientific, and Medical Radio Frequency Generators.”
European Safety
and EMC
Standards
Safety
This instrument meets European requirements for safety (Low Voltage Directive
2006/95/EC). This instrument has been tested to and complies with standards
EN 61010-1:2001, “Safety Requirements for Electrical Equipment for Measurement,
Control and Laboratory Use, Part 1: General Requirements.”
EN 61010-2-010, “Particular Requirements for Laboratory Equipment for the
Heating of Materials.”
EN 61010-2-081, “Particular Requirements for Automatic and Semi-Automatic
Laboratory Equipment for Analysis and Other Purposes.”
EMC
This instrument meets European requirements for emission and immunity (EMC
Directive 2004/108/EC). This instrument has been tested to and complies with
standard EN 61326 (Group 1, Class B), “Electrical Equipment for Measurement,
Control and Laboratory Use – EMC Requirements.”
Australian EMC
Standards
This instrument has been tested to and complies with standard AS/NZS 2064,
“Limits and Methods Measurement of Electromagnetic Disturbance Characteristics
of Industrial, Scientific, and Medical (ISM) Radio-frequency Equipment.”