ARA P Series User manual
DOC. No: AP7OMPDEN2001
UM-H_ARA P170(E)(Rev.1), 20210819
USER MANUAL CO2 INCUBATOR
Contents
1. Safety Consideration
1-1. General Precautions
1-2. Pictograph
1-3. Electrical Precautions
2. Product Description
2-1. Exterior Description
2-2. Product Components
2-3. Technical Specification
3. Product Installation
3-1. Caution during Installation
3-2. Caution prior to Use
3-3. Preliminary Cleaning
3-4. Installing the Shelf Channels
3-5. Installing the Humidity Pan
3-6. Installing the Shelves
3-7. Connecting the CO2 Gas Supply
3-8. Connecting Power
3-9. Connecting External Device Port
4. Usage Instruction
4-1. Display
4-2. Status Indicators
4-3. Temperature Configuration
4-4. CO2 Concentration Configuration
4-5. History
4-6. Time Configuration
4-7. Sterilization
5. Maintenance
5-1. Interior
5-2. Exterior
6. Troubleshooting
6-1. Before Contacting Us
6-2. Error Message
USER MANUAL CO2 INCUBATOR
1. Safety Considerations
1-1. General Precautions
The following User Manual describes product functions, usage procedures and safety
precautions during use. Read this instruction manual thoroughly before use.
1. Locate the instrument on a flat and leveled location.
2. Do not move the instrument during use.
3. Check the voltage before connecting power to avoid damaging the instrument due to incorrect voltage.
4. Before connecting the power, check the rated voltage.
5. Only use accessories and components provided or approved by Hanil.
6. Only insert designated container or sample do not insert anything else.
7. Avoid using the instrument in a flammable or radioactive environment.
8. Do not insert volatile or flammable substance inside the instrument.
9. For CO2 supply, it is recommended to use gas regulator endorsed by Hanil at below 0.9 kgf/cm3 (13PSI).
Connect the gas supply correctly to avoid CO2 leakage or exceeding permitted CO2concentration range.
10. If unusual odor or smoke occurs, immediately disconnect the main power and contact Hanil Technical
Support Team.
11. Do not remain the door open for a rolonged period during use.
12. Do not rest other objects on top of or next to the instrument.
13. Avoid using organic solvent and instead use neutral detergent for cleaning.
14. Always disconnect the power supply prior to cleaning.
15. Remove water from humidity pan and unplug the power cord when the instrument is to remain unused
for an extended period.
16. All repairs and internal maintenance must be performed by qualified service personnel from Hanil.
17. Thoroughly remove any contamination when requesting repair or maintenance.
18. Do not use multiple instruments simultaneously when plugged to multi-outlets.
19. Do not touch instrument or power plug with wet hands.
20. Do not apply excessive force to the instrument.
21. Contact Hanil regarding instrument repair.
USER MANUAL CO2 INCUBATOR
1-2. Pictograph
General Danger
Potential electrical hazards
1-3. Electrical Precautions
1. Only use power cable provided with the product.
2. Plug the power cable to a grounded outlet.
- To check whether the outlet is grounded, contact a professional electrician or
Hanil Technical Support Team.
3. Install the instrument in a way that prevents the power cable from being stepped.
4. Do not rest any objects on top of the power cable.
5. If the following cases occur, turn off the instrument immediately, unplug the power cable
from the outlet and contact Hanil Technical Support Team.
- The instrument makes unusual noise or odor
- The power cable is worn out or damaged.
- Spilled liquid on the instrument
- Water entered the instrument
- Parts of the instrument is damaged
USER MANUAL CO2 INCUBATOR
2. Product Description
2-1. Exterior Description
USER MANUAL CO2 INCUBATOR
2-2. Product Components
- When the product arrives, check if there exists any damage to the package.
- If there is damage on the package, contact the vendor immediately.
- Contacts are located on the bottom of the user manual and the label on the
product exterior.
1. After purchase, open the packing box and confirm that everything under the component list is
present.
Components List
Quantity
Content
1
Main Unit
1
User Manual
1
AC Power Adapter (180mm)
1
Humidity Pan
2
Shelf Channels (Left, Right)
3
Shelves
3
CO2 Supply Pipe
2-3. Technical Specification
Type
Content
Model Name
ARA P170
Instrument Dimension (WxDxH)
726 x 651 x 935mm
Chamber Dimension (WxDxH)
560 x 470 x 650 mm
Shelf Dimension (WxDxH)
536 x 461 x 25 mm
Weight
96 kg
Interior Volume
170L
Cabinet Material
Stainless Steel
Inner Door Material
Tempered Glass
Shelf Material
Stainless Steel
LCD Size (Resolution)
7 inch (WVGA, 800 x 480)
Heating Method
Directing Heating
Humidifying Method
Pan Evaporation
Temperature Control
Microprocessor PID Controlled 7-Channel Temperature
Sensor (PT100) (Resolution: 0.1°C)
CO2 Concentration Control
Microprocessor PID Controlled Non-Dispersive Infrared
USER MANUAL CO2 INCUBATOR
Sensor (Resolution: 0.1%)
Configuration Display
Digital Display
In-Chamber Air Circulation
Fan-less Passive Ventilation
Alarm
Temperature, CO2 Concentration
CO2 Supply Tube Outer Diameter
12 mm
CO2 Supply Pressure
0.9 (Less Than 13 PSI)
CO2 Fill Inner Diameter
8 mm
Sterilization
Dry Sterilization (120C)
Accessories
AC Power Code (1), Humidity Pan (1), Shelves (3), Shelf
Channels (2), CO2 Supply Pipe (3m)
Electrical Specification
220 V (50/60)
Max Power Consumption
1.1 kW
Temperature Control Range
Room Temperature +5~50 °C
Temperature Stability
±0.2°C (@ 37°C)
Temperature Uniformity
±0.3°C
Temperature Recovery Time
Within 10 minutes (recovery to 37°C after opening the inner
door for 30 seconds)
CO2 Control Range
0~20%
CO2 Stability
±0.1% (at 5% set concentration)
CO2 Uniformity
±0.1%
CO2 Recovery Time
Within 10 minutes (recovery to 5% after opening the inner
door for 30 seconds)
Humidity Range in Chamber
95%
Usable Ambient Temperature
14~32°C
Usable Relative Humidity
~80%
Maximum Shelf Capacity
15 kg
Appropriate Shelf Capacity Ratio
~50%
USER MANUAL CO2 INCUBATOR
3. Product Installation
3-1. Caution during Installation
- Improper installation can cause malfunction during use.
- Contact Hanil Technical Support Team when the instruments need to be moved and reinstalled.
Install the instrument on a firm level surface.
During installation, maintain a 15cm clearance behind the instrument and 10cm
ventilation space on each side of the unit.
Plug the AC power cord attached to the back of the instrument into a grounded
socket.
Do not place flammable or explosive substance next to the instrument.
Avoid installing in an environment containing flammable or corrosive gas.
Avoid installing in a crowded, hot and humid environment or in front of a cooling
and heating system to prevent contamination due to airborne microorganism.
Installing in a cleanroom is recommended for the most effective culturing condition.
Do not install the instrument in a humid or wet location to avoid short circuits or
electrical injury.
3-2. Caution prior to Use
Use in an environment with ambient temperature between 14°C and 30°C and relative
humidity below 80%. Using the instrument outside of permissible environment can
cause malfunction or condensation within chamber.
Avoid frequently opening and close inner and outer door. Doing so extends the period
that the instrument stays below set temperature and CO2 concentration.
Do not insert excessive number of culture dish. Doing so can disturb air circulation
and increase temperature. Using up to 50% of the shelf surface is recommended.
Pre-run the instrument for 1~2 hours before cell culture.
Do not pull the power plug during use.
Secure the accessories after checking interior condition, shelf and humidity pan
location.
USER MANUAL CO2 INCUBATOR
3-3. Preliminary Cleaning
Sterilize and disinfect the chamber and the accessories prior to first use.
Clean all components with neutral detergent and rinse with distilled water.
Clean the chamber and its components with clean and soft cloth soaked with 70%
ethanol
Do not use corrosive agents such as sodium chlorate or halogen solutions.
Do not use cleaning tools with rough surface to avoid scratching the instrument
surface.
3-4. Installing the Shelf Channels
Follow the instruction below when reinstalling shelves support due to cleaning.
USER MANUAL CO2 INCUBATOR
3-5. Installing the Humidity Pan
Only use sterilized distilled water. Do not use ionize water to prevent corroding the
humidity pan
3-6. Installing the Shelves
1. Place the latch attached to the bottom of the shelf below the shelf Channels and push the shelves
into the chamber.
USER MANUAL CO2 INCUBATOR
3-7. Connecting the CO2 Gas Supply
Close the CO2 tank valve when connecting or disconnecting CO2 supply tube.
3-8. Connecting Power
Make sure that the power source match the electrical specification on this instrument
(220V, 50/60 Hz).
[Turning On]
1. Connect the AC power cord to the power socket located on the rear left of the
instrument. Then, connect the power plug into the power outlet.
2. Click the [I] from the [I/O] of the power switch, located at top right of the instrument,
to turn on.
[Turning Off]
1. Press the [I] from the power switch to turn off.
USER MANUAL CO2 INCUBATOR
3-9. Connecting External Device Port
Use the external device port to install external devices such as additional sensors or connect cables
to and from the chamber.
Please enclose port with silicon cap after connection. Opening the port can cause micro-
condensation around the port.
Outer device connection
USER MANUAL CO2 INCUBATOR
4. Usage Instruction
4-1. Display
Door Indicator: Displays outer door status
Graph Indicator: Displays Set Temperature, Current Temperature, Set CO2 Concentration,
and Current Co2 Concentration in real time.
Status Indicator: Displays current operation status and error message
Temperature Indicator: Displays Set and Current Temperature. Touch “Temp.” to set
temperature.
CO2 Indicator: Displays Set and Current CO2 Concentration. Touch “CO2 Lv” to set CO2
Concentration.
Alarm Indicator: Turn alarm sound ON/OFF
Menu: Displays Operation Log, Date/Time Setting and Sterilization function.
USER MANUAL CO2 INCUBATOR
4-2. Status Indicators
[Temperature]
Shows change in temperature over time in a purple graph.
> Temperature Display Range: 0~50°C
[CO2 Concentration]
Shows change in CO2 concentration over time in a blue graph.
> CO2 Concentration Display Range: 0~20%
[All View –Cumulative Temperature/Concentration]
Press “Graph Indicator” and “All View” to view change in temperature and CO2
concentration over 240-hour period.
USER MANUAL CO2 INCUBATOR
[Export –Data Export]
Press “Graph Indicator” and “Export” to save temperature and CO2 concentration data in
the past 240 hours into an USB.
[Clear –Delete Data]
Press “Graph Indicator” and “Clear” to delete and reset the collected data in the past 240
hours.
4-3. Temperature Configuration
1. Press “Temp.” form the main screen.
USER MANUAL CO2 INCUBATOR
2. Input the desired temperature and press
“Enter”. Set temperature has range of 0~50°C.
When input temperature exceeds the
maximum set temperature, 50°C, temperature
is set to 50°C.
4-4. CO2 Concentration Configuration
1. Press “CO2 LV” form the main screen.
2. Input the desired CO2 concentration and
press “Enter”. Set concentration has range of
0~20%. When input concentration exceeds
the maximum set value, 20%, concentration
is set to 20%.
4-5. History
1. Press “Menu” from the main screen.
USER MANUAL CO2 INCUBATOR
2. Select “History view”
3. The following screen displays the operation log in a chronological order.
Press “Export” to save log into USB
> Log saves up to 40 lines regardless of time.
4-6. Time Configuration
1. Press “Menu” form the main screen.
USER MANUAL CO2 INCUBATOR
2. Select “Clock Setting”
3. Configure each part of time under Year/Month/Day/Hour/Min/Sec by dragging scroll
bars up and down. Press “Save” to finalize the configuration.
4-7. Sterilization
1. Press “Menu” from the main screen.
2. Select “Sterilization”
USER MANUAL CO2 INCUBATOR
3. Screen displays current Chamber temperature. Press “Start” to open “Sterilization” page.
Sterilization is set at 120°C for 4 hours.
Press “Yes” when Sterilization confirmation window opens to start Sterilization.
-Before Sterilization, remove CO2 Sensor and clean the Chamber.
-Start Sterilization only after removing liquid (sample, water etc.) from the Chamber.
-Refer to the following page to remove CO2 Sensor.
* CO2 Sensor Removal
Pull the CO2 Sensor according to the diagram to remove.
4. Once Sterilization starts, the chamber reaches up to 120°C.
After reaching 120°C, a message saying “Maintaining chamber at 120°C” appears and
remains for 4 hours.
USER MANUAL CO2 INCUBATOR
5. After 4 hours of process, a message saying “Cooling down to set temp” appears and returns
to set temperature before Sterilization. After returning to set temperature, “Complete the
sterilization” message appears and Sterilization will be over.
Press “Home” to return to main screen.
*CO2 Sensor Insertion
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