Awareness Technology Stat Fax 2200 User manual

Stat Fax®2200
Microplate Incubator/Shaker
Operator’s Manual
© Awareness Technology, Inc. 2001 Revised 2018 Doc. 2200 Rev. H

Contents
1. Introduction ...................................................................................................... 1
1.2 Safety Symbols Le Symboles de Sûreté ................................................................................. 1
1.3 Safety Terms Terminologie de Sûreté ..................................................................................... 1
1.4 Safety Precautions................................................................................................................... 2
2. Installation........................................................................................................ 3
3. Power Requirements ....................................................................................... 3
4. Principle of Operation ..................................................................................... 4
5. Specifications................................................................................................... 5
6. Operating Procedures ..................................................................................... 6
6.1 Incubating................................................................................................................................. 6
6.2 Mixing....................................................................................................................................... 7
6.3 Timing ...................................................................................................................................... 7
6.4 Other Functions ....................................................................................................................... 8
6.4.1 The Clear Key ................................................................................................................... 8
6.4.2 The Pulse Mode ................................................................................................................ 8
6.4.3 Condensation .................................................................................................................... 8
7. Additional Tips and Information ..................................................................... 8
7.1 Maintenance............................................................................................................................. 8
7.1.1 Exterior .............................................................................................................................. 8
7.1.2 Avoid Humidity .................................................................................................................. 8
7.2 Accessories.............................................................................................................................. 8
7.2.1 Temperature Probe Plate .................................................................................................. 8
8. Information ....................................................................................................... 9

Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H1
1. Introduction
Stat Fax® 2200 is an economical combination incubator, timer, and shaker that accommodates two standard
microplates or strip trays. This microprocessor controlled instrument offers variable temperatures, times, and
mixing speeds via the keypad. Incubation, timing, and mixing may also be used independently. A pulse mode is
available. Temperatures may be set from room temperature to 40°C with a resolution of 0.1 degree. The
instrument is designed to provide long life and trouble-free performance. A smoke tint acrylic cover protects the
plates, shields them from light, and insulates them during incubation. The enclosed system also reduces the risk
of biohazard from the dispersal of aerosols. Like all of the Stat Fax®instruments, this instrument offers quality
and versatility and is backed with a one year warranty.
1.2 Safety Symbols Le Symboles de Sûreté
Symbols that may appear on the product:
Les symboles de sûreté peuvent apparaitre sur le produit:
WARNING
AVERTISSEMENT
Protective Ground
La Terre Electrique
CAUTION
L’ATTENTION
BIOHAZARD
BIOHAZARD
Risk of Shock
Risque de Choc
(Earth) Terminal
Prise de Terre
Refer to Manual
Se Rapportent a Manuel
Risk of Infection
Risque d’infection
1.3 Safety Terms Terminologie de Sûreté
These terms may appear on the product: Les marques sur le produit:
These terms may appear in this manual: Les marques dans l’opérateur manuel:
DANGER
DANGER Le “de marque: DANGER”
Indicates an injury immediately accessible as you read this marking
Indique le risque immédiat de dommages (assessible tandis que vous lisez la
marque)
WARNING
AVERTISSEMENT! Le “de marque:
WARNING”
WARNING statements identify conditions or practices that could result in injury
or loss of life. WARNING indicates an injury hazard not immediately accessible
as you read this marking.
Ces rapports identifient les conditions ou les pratiques qui pourraient avoir
comme conséquence les dommages ou les pertes humaines.
CAUTION
L’ATTENTION “Le de marque:
CAUTION”
CAUTION statements identify conditions or practices that could result in
damage to this product or other property.
Ces rapports identifient les conditions ou les pratiques qui pourraient avoir
comme conséquence les dommages a ce produit ou a toute autre propriété.
BIOHAZARD
BIOHAZARDS are biological agents that can cause disease in humans. Lab
workers handling potentially infectious materials must use universal precautions
to reduce the risk of exposure to these agents.

2Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H
1.4 Safety Precautions
To assure operator safety and prolong the life of your instrument, carefully follow all instructions outlined below.
Read Instructions
Take time to read this manual carefully before using this instrument. Review the
following safety precautions to avoid injury and prevent damage to this instrument
or any products connected to it. To avoid potential hazards, use this instrument
only as specified. For best results, familiarize yourself with the instrument and its
capabilities before attempting any clinical diagnostic tests. Refer any questions to
your instrument service provider.
Servicing
There are no user-serviceable parts inside the instrument. Refer servicing to
qualified service personnel. Use only factory-authorized parts. Failure to do so
may void the warranty.
Wear Protective Apparel
Many diagnostic assays utilize materials that are potential biohazards.
WARNING: Always wear protective apparel and eye protection while using this
instrument.
Follow Operating Instructions
WARNING: Do not use this instrument in a manner not specified by the manual, or
the protection provided by the instrument may be impaired.
Use Proper Power Cord
WARNING: Use only the power cord specified for this product and certified for the
country of use.
Observe All Terminal Ratings
WARNING: To avoid fire or shock hazard, observe all ratings and markings on the
instrument. Consult this manual for further ratings information before making
connections to the instrument.
Install as Directed
The instrument should be installed on a sturdy, level surface capable of safely
supporting the instrument’s weight 7 lbs (3.1kg). The mounting surface should be
free of vibrations.
Provide Proper Ventilation
Refer to the installation instructions for details on installing the product so it has
proper ventilation. The instrument should be surrounded by the following
clearances: 8cm (3”) around perimeter of unit, 8cm on top, and 1.27cm bottom
(1/2”).
Do Not Operate Without Protective
Covers
WARNING: Do not operate this instrument with covers and panels removed.
Avoid Exposed Circuitry
WARNING: Do not touch exposed connections and components when power is
present.
Avoid Excessive Dust
Do not operate in an area with excessive dust.
Do Not Operate With Suspected
Failures
WARNING: If you suspect there is damage to this instrument, have it inspected by
a qualified service person.
Do Not Operate in Wet/Damp
Conditions
WARNING: Do not operate instrument in wet/damp conditions.
Do Not Operate In An Explosive
Atmosphere
WARNING: Do not operate instrument in an explosive atmosphere.

Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H3
Safety Precautions (Continued)
Operating Precautions
Be sure to run a sufficient number of controls in each assay. If controls are not within their
acceptable limits, disregard test results.
Keep Instrument Surfaces
Clean and Dry
CAUTION: Solvents such as acetone or paint thinner will damage the instrument.
•Do not use solvents to clean the unit. Avoid abrasive cleaners; the display overlay is
liquid-resistant, but easily scratched.
•Clean the exterior of the instrument with a soft cloth using plain water. If needed, a
mild all-purpose or nonabrasive cleaner may be used.
•Use as a disinfectant a 10% solution of chlorine bleach (5.25% Sodium Hypochlorite)
or 70% isopropyl alcohol
•Take special care not to spill liquid inside the instrument
CAUTION! L’ATTENTION!
BIOHAZARD: If any materials are overturned during operation, immediately set
the power switch to OFF. This material should be treated as potentially
biohazardous. Appropriate cleanup and disposal of biohazardous waste should
be used.
Avertissement! Lors du fonctionnement, si on renverse des matériaux, coupez
immédiatement le courant. Placez le commutateur électrique a AU LOIN(0). Traitez le
matériel comme biohazardous, utilisant approprie nettoient et des méthodes de
disposition.
2. Installation
Carefully unpack the instrument and remove it from its plastic bag. Report any obvious damage to your freight
carrier at once.
Note: Retain the original packing materials for future use in the event that the instrument is shipped to another
location or returned for service.
Place the instrument on a flat working surface capable of safely supporting the weight of the instrument
(approximately 7 lbs). A clearance of at least 8 cm around the instrument is required to assure optimal ventilation.
3. Power Requirements
Either of the following power units may be used:
120VAC input, 12VAC, 2 Amp output
230VAC input, 12VAC, 2 Amp output
Use a power unit approved for the country of use. For use inside the US: Use only a NRTL listed plug-in Class 2
transformer with rated output 12VAC, 2A.
A power unit is supplied with the instrument. This type of power unit is commonly referred to as a wall
transformer, direct plug-in transformer, power adaptor, or simply an adaptor and utilizes an isolation transformer.
Plug the power unit into the instrument jack on the rear panel, and into the wall outlet. The ON/OFF switch is
located on the back of the instrument. When you turn the instrument on, you will see "rdY" (indicating READY) on
the instrument display.

4Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H
4. Principle of Operation
Six heating resistors connected to the base plate warm the inner compartment. The temperature of the air in the
compartment is constantly monitored at the height of the wells by a thermistor located between the two trays. A
circulating fan serves to remove any condensation from the inner lid. The double lid system insulates the plates
from changes in ambient temperature.
Four flexible legs support the tray. A microprocessor controls the speed of an orbiting counterweight mechanism
that shakes the tray. The user may select any one of 8 speeds ranging from 575 rpm to 1500 rpm.
The following diagram describes the key functions for the instrument. More details on the operation of each
feature are provided in Section 8, Operating Procedures.
YES and NO keys are for possible future options, but are not currently used.
NOTE: Many keys have more than one function. The numbers apply only to setting timer, temperature, and
speed. Otherwise, the function which appears above the key defines the function of the key.
5 PULSE – provides a 3
second burst at highest mixing
speed
4 VIEW TIME – displays time
remaining on the timer
7 VIEW TEMP – displays the
current temperature of the
wells
2– Decreases
mix speed
3 STOP –
Stops the mixer
6– Manually
operates the fan
MIX – starts mixing
and displays the
current mixing speed
SET TIME – sets the
timer
SET TEMP – sets the
temperature
ENTER – inputs time
and temperature
Use DECIMAL POINT
between minutes and
seconds
CLEAR – corrects key entry mistakes,
clears display, or cancels timer and alarm.
Press CLEAR twice to exit without
changing settings.
1– Increases
mix speed

Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H5
5. Specifications
Incubator
Temperature range: Ambient to 40°C
Resolution ......................................................................... 0.1°C
Uniformity with cover: ....................................................... better than +/- .4°C
Mechanism: ...................................................................... enclosed warmed aluminum bed with air circulation
Warm up time: .................................................................. 15 minutes typical, display temperature to check
Temperature detector: ...................................................... thermistor, feedback, heuristic software
Mixer
Speeds:............................................................................. 8 mix speeds, from 575 to 1500 rpm, timed duration on
continuous and mix pulse, 1500 rpm, for 3 second
duration.
Mechanism: ...................................................................... orbital mixing by rotating counterweight
Timer
Modes: .............................................................................. set time, view time remaining, audible signal, stop mixing
(maintains temperature control after time out)
Resolution:........................................................................ one second
Range: .............................................................................. one second to 99 minutes 59 seconds
Electronic
Display: ............................................................................. four, 7-segment, red LEDs
Keypad:............................................................................. 16-key, domed membrane switch, enunciating, 4x4
Power requirements:......................................................... 12V AC, 50-60Hz, 2 amp, power unit available for 120V
or 230V power sources. Use power unit approved for
country of use.
Microprocessor: ................................................................ Z80A
Certifications and Compliances: ....................................... NRTL Listed CE Certification
Recommended Environmental Condition:
Indoor Use
Mains supply voltage: ....................................................... Fluctuations not to exceed ±10% of the nominal voltage
Operating Temperature: ................................................... 18-35°C recommended
Operating Humidity: .......................................................... Less than 85% recommended
Other
Vessel: ............................................................................ holds 2 standard microtiter plates or strip trays
Enclosure: ....................................................................... fire retardant ABS plastic enclosure with metal base
Aerosol guard:................................................................. smoke-tint acrylic cover
Dimensions: .................................................................... approx. 10 x 12½ x 6 inches (26 x 32 x 16 cm) with
cover, 7 lbs. (3 kg)
NOTE: Although it may be safe to operate in these conditions, it may not be
suitable for the performance of your tests. Check with your reagent supplier.

6Stat Fax®2200 Operator’s Manual Doc. 2200 Rev. H
6. Operating Procedures
1. The ON/OFF switch is located on the back of the instrument.
2. Lift off the smoked acrylic cover. Open the internal cover by pulling back on the metal clip while lifting the
front of the clear plastic lid. The covers serve as aerosol guards as well as insulators and should be used at all
times.
3. The instrument is designed to hold two standard 96-well microplates or strip trays, round or flat bottom wells.
To properly seat a microplate in the Incubator/Shaker, slide the top edge of the microplate into the silicon
bumpers located in the back two corners of the plate holder. By applying pressure to these bumpers with the
plate, the bottom edge of the plate should click into place. Press BOTH plates into the bumpers located at the
rear corners of the metal frames in the incubation platform. Close the internal cover and replace the outer cover.
6.1 Incubating
To set the incubator temperature, press the SET TEMP key. It will display the previously set temperature. If this
is the desired temperature, press ENTER; otherwise, type the desired temperature in Celsius, using the numeric
keys, with no more than one place after the decimal point. Press the ENTER key. The instrument will pause to
measure and then display the current temperature. (The displayed temperature is corrected to reflect the
equilibrated temperature inside a micro-well containing 200µL of water.) Then, either heating will begin or heating
will stop depending on the set temperature, until the desired temperature is reached. For faster cooling, open
both lids and press the “6” key to turn on the fan. The display will continue to show the incubator temperature,
unless another function is selected. A decimal point indicator under the degree symbol reflects the state of the
heater. When it is lit the heater is on. When first set, the instrument may over and undershoot the desired
temperature but will quickly adjust and hold the temperature constant. When desired temperature is achieved, the
heat will pulse on and off to maintain the temperature within +/- 0.5 degrees. The fan may run periodically during
heating to prevent water condensation on the internal lid.
To check the temperature when it is not being displayed, press the VIEW TEMP key. The temperature will then
be continuously displayed until another function is selected.
If no temperature is entered the unit automatically controls the temperature to 25°C or ambient, whichever is
greater.