Barnstead International Imperial III 1464 Series User manual

LT1465X3 • 3/14/06
Imperial III General Purpose
Incubators
Series 1464, 1465, 1466
Model No.
Gravity Convection:
302, 302-1
305, 305-1
310, 310-1
Mechanical Convection:
305M, 305M-1
310M, 310M-1

Table of Contents
Description ..........................................................................................................................................................3
Safety Information ..............................................................................................................................................4
Alert Signals..................................................................................................................................................4
Specifications ......................................................................................................................................................5
Electrical Requirements................................................................................................................................5
Temperature Range ......................................................................................................................................5
Shipping Weight............................................................................................................................................5
Unit Dimensions............................................................................................................................................5
Environmental Conditions ............................................................................................................................6
Unpacking and Installation ..................................................................................................................................7
Shipping Carton ............................................................................................................................................7
Location ........................................................................................................................................................7
Leveling ........................................................................................................................................................7
Shelf Installation ..........................................................................................................................................7
Operation ............................................................................................................................................................9
Control Panel ................................................................................................................................................9
Start-Up ........................................................................................................................................................9
Setting Operating Temperature Thermostat ..............................................................................................10
Temperature Controller ..............................................................................................................................10
Auto Tune....................................................................................................................................................11
Temperature Calibration ............................................................................................................................12
Troubleshooting ................................................................................................................................................14
Maintenance......................................................................................................................................................15
Checking Controls ......................................................................................................................................15
Cleaning......................................................................................................................................................15
Care and Cleaning of Stainless Steel ........................................................................................................15
The Alloy Called Stainless ..........................................................................................................................15
Stainless Guidelines ..................................................................................................................................16
The pH Factor ............................................................................................................................................16
Special Considerations ..............................................................................................................................17
Cleansing Agents........................................................................................................................................17
Cleaning Methods ................................................................................................................................17
Materials Effective in Disinfecting ........................................................................................................18
Servicing Guide ................................................................................................................................................19
Heater Replacement ..................................................................................................................................19
Thermostat Replacement ..........................................................................................................................19
Switch or Status Lamp Replacement ........................................................................................................19
Replacing Temperature Controller ..............................................................................................................20
Replacement Parts............................................................................................................................................21
Wiring Diagram..................................................................................................................................................22
Ordering Procedures ........................................................................................................................................24
Decontamination Statement..............................................................................................................................25
Two Year Limited Warranty ..............................................................................................................................28
2

Description
Model 302
Barnstead|Lab-Line Imperial III General Purpose
Incubators are useful in all types of general incubating
and paraffin imbedding.
Cabinets are made of heavy-gauge steel with a powder-
coated finish for optimum appearance and easy cleaning,
while the interior walls are stainless steel to spread warm-
wall radiant heat evenly throughout the chamber. Heaters
are in direct contact with chamber walls to allow close
temperature control and quick recovery after door open-
ings. This arrangement also minimizes temperature gra-
dients and offers a large working area in the chamber.
Adouble set of doors, the inner of tempered glass, per-
mits unobstructed viewing of chamber contents without
disturbing the interior environment. Slide-out shelving
can be positioned to meet user requirements.
APID microprocessor based controller maintains cham-
ber temperature. An over-temperature safety thermostat
controls temperature in the event of primary control fail-
ure. A status lamp above each control is lit when the
respective control is maintaining power to the heaters.
Incubators are available in three sizes. A grounded 3-
prong convenience outlet is supplied in midsize model
chambers and 2 outlets are installed in larger models.
Models are built for either 120 VAC or 240 VAC power
requirements. Models 305M and 310M have the same
characteristics as those listed above but, in addition, pro-
vide mechanical convection. These models use a blower
and plenum design to circulates the air within the cham-
ber that allows for rapid temperature and uniformity recov-
ery after door openings.
3

Your Barnstead|Lab-Line Imperial III General Purpose
Incubator has been designed with function, reliability, and
safety in mind. It is your responsibility to install it in con-
formance with local electrical codes. It is most important
that the user follow installation instructions exactly as writ-
ten. Failure to do so is likely to lead to improper opera-
tion, erroneous calibrations and possible damage to the
equipment. Do not attempt operation without this informa-
tion.
Safety Information
Warning
Warnings alert you to a possibility of
personal injury.
Caution
Cautions alert you to a possibility of
damage to the equipment.
Note
Notes alert you to pertinent facts and
conditions.
Hot Surface
Hot surfaces alert you to a possibility of
personal injury if you come in contact
with a surface during use or for a period
of time after use.
Alert Signals
4

Electrical Requirements
Voltage Hertz Amps Watts
302 120 50/60 2.1 400
302-1 240 50/60 0.7 400
305 120 50/60 3.3 400 (1 electrical outlet, 120V, 500W total load)
305M 120 50/60 3.7 400 (1 electrical outlet, 120V, 500W total load)
305-1 240 50/60 1.7 400 (1 electrical outlet, 120V, 500W total load)
305M-1 240 50/60 1.7 400 (1 electrical outlet, 120V, 500W total load)
310 120 50/60 5.0 600 (1 electrical outlet, 120V, 500W total load)
310M 120 50/60 5.8 600 (1 electrical outlet, 120V, 500W total load)
310-1 230/240 50/60 2.5 600 (1 electrical outlet, 120V, 500W total load)
310M-1 230/240 50/60 2.5 600 (1 electrical outlet, 120V, 500W total load)
Temperature Range
Slightly above ambient to 65°C
Shipping Weight
302 Models: 75 lbs. (34 kg)
305 Models: 120 lbs. (55 kg)
310 Models: 215 lbs. (98 kg)
Unit Dimensions
INSIDE OUTSIDE
302 Models: 13"W x 17"D x 20"H 16"W x 21"D x 29.5"H
(33 x 43 x 51 cm) (41 x 53 x 75 cm)
305 Models: 17"W x 21"D x 25"H 20"W x 25"D x 34.5"H
(43 x 53 x 64 cm) (51 x 64 x 88 cm)
310 Models: 37"W x 21"D x 25"H 40.5"W x 25"D x 34.5"H
(94 x 53 x 64 cm) (103 x 64 x 88 cm)
USABLE VOLUME SHELVES SHELF AREA
302 Models: 2.6 cu. ft. 2 2.8 sq. ft.
305 Models: 5.2 cu. ft. 3 6.9 sq. ft.
310 Models: 11.2 cu. ft. 6 13.8 sq. ft.
Specifications
5

SPECIFICATIONS
Environmental Conditions
Operating: 15°C to 40°C; 20% to 80% relative humidity, non-condensing.
Installation category II (overvoltage) in accordance with IEC 664.
Pollution degree 2 in accordance with IEC 664.
Altitude Limit: 2,000 meters.
Storage: -25°C to 65°C
10% to 85% relative humidity
6

Shipping Carton
The shipping carton should be inspected upon delivery.
When received, carefully examine for any shipping dam-
age before unpacking. If damage is discovered, the deliv-
ering carrier should both specify and sign for the damage
on your copy of the delivery receipt.
Open the carton carefully making certain that all parts are
accounted for before packaging materials are discarded.
After unpacking, if damage is found promptly report it to
the carrier and request a damage inspection promptly.
IMPORTANT: Failure to request an inspection of damage
within a few days after receipt of shipment absolves the
carrier from any liability for damage. You must call for a
damage inspection promptly.
Location
Place the unit on a level surface protected from drafts,
strong air currents and large fluctuations in ambient tem-
perature. It must be located near an electrical outlet that
meets the unit nameplate requirements. Allow clearance
around the unit for free air convection, accessory equip-
ment, and user convenience. Do not cover the vent on
top of the incubator.
Leveling
It is important that the unit is level before operation. DO
NOT REMOVE THE UNIT’S RUBBER FEET—they assist
proper air circulation inside and outside of the chamber.
Removal can result in erratic control and excessive heat
build-up beneath the unit.
Shelf Installation
1. Each shelf bracket has 2 prongs at each end for
attachment to the chamber sidewall. Insert the
longer top prongs into slots of equal height on
one side of the chamber, then push the bracket
up and insert the lower prongs.
2. Push down on the bracket to insure proper seat-
ing.
Unpacking and Installation
Note
Leave unit disconnected when not in
use.
7

3. Repeat this procedure for the opposing bracket
making sure it is on the same level as the first
bracket (count the slots).
4. Install other shelf brackets in the same way then
slide shelves into place.
UNPACKING AND INSTALLATION
8

1. POWER SWITCH: This 2-position rocker switch
controls power to entire unit. The switch is lit
when power is ON.
2. CONTROL STATUS LAMP: This lamp is lit when
power is being supplied to heater and not lit
when power to the heater is OFF.
3. PROGRAMMABLE CONTROLLER: Maintains
chamber temperature and provides a display of
either chamber or set point temperatures.
4. OVER-TEMPERATURE STATUS LAMP: This
lamp is lit when either an over-temperature con-
dition exists or the over-temperature thermostat
is improperly set too low.
5. OVER-TEMPERATURE THERMOSTAT: This
hydraulic thermostat is set by the operator to
back up the control thermostat and safeguard
the incubator.
Start-Up
Insert a flat-blade screwdriver into the panel opening and
rotate the over-temperature thermostat stem completely
clockwise. After making sure the unit is plugged in, press
the power switch to ON.
1 2 3 4 5
Operation
Control Panel
Warning
Do not use in the presence of flam-
mable or combustible materials or
explosive gases. Do not use in the
presence of pressurized or sealed
containers. Fire or explosion may
result, causing death or severe
injury.
Warning
Do not heat any substance above a
temperature that will cause it to emit
toxic fumes. Death or severe injury
may result.
9

Setting the Over-Temperature
Thermostat
After the unit has reached set point, rotate the over-tem-
perature thermostat stem counterclockwise until the over-
temperature status lamp (4) is lit. Then rotate it clockwise
approximately 5 degrees past the point at which the lamp
is no longer lit. At this point, the over-temperature ther-
mostat will control heaters only at temperatures above the
controller set point.
Temperature Controller
1. CONTROLLER SELF-TEST: When the incuba-
tor is powered up the controller will display 8888
along with the three decimal points and the heat
ON indicator lamp. The display will then blank
out for 2 seconds before showing the chamber
temperature.
2. HEAT ON INDICATOR: The heat ON indicator
lamp is lit when the chamber heater is receiving
power. The lamp will normally flash when the
chamber temperature is at set point.
OPERATION
✱▼
▼
65.0
Heat Indicator
Chamber or Setpoint Temperature
10

3. SET POINT ADJUSTMENTS: The temperature
controller normally displays the chamber tem-
perature. To view or change the temperature
set point proceed as follows:
A. Press and hold the “STAR” (✳) key and use
either the UP or DOWN arrow key to adjust the
set point to the desired temperature. Release
the “STAR” (✳) key.
B. Allow at least 30 minutes for the chamber tem-
perature to stabilize.
Auto Tune
The auto tune program automatically adjusts the con-
troller parameters to achieve optimal temperature control.
It is not necessary to run the auto tune program when
setting up the oven. However, if the temperature appears
to be unstable, the auto tune program can be run using
the procedure shown below:
For Best Results
• Set the usual set point temperature and use nor-
mal load conditions.
• Allow the oven to stabilize at set point for at
least 30 minutes.
Auto Tuning Procedure
1. Enter the program mode by pressing and hold-
ing BOTH the UP and DOWN arrow keys for 3
seconds.
2. Release BOTH arrow keys when tunE is dis-
played.
OPERATION
Press Controller
✳View setpoint
✳▼ Decrease setpoint
✳▲ Increase setpoint
11

3. The controller display should now be alternating
between tunE and oFF.
4. Press and hold the “STAR” (✳) key. Press and
release the up arrow key until At.SP is dis-
played. Release the “STAR” (✳) key.
5. After one minute has elapsed, the controller dis-
play will begin to alternate between showing the
chamber temperature, tunE and At.SP.
6. Allow the program to run until the display again
shows only the chamber temperature.
Temperature Calibration
1. Place a calibrated thermometer in the approxi-
mate geometric center of the chamber in a posi-
tion that would allow it to be read through the
glass door.
2. Press and hold the “STAR” (✳) key and using
the UP or DOWN arrow key, adjust the set point
to the desired temperature.
3. Allow the unit to run for at least 30 minutes.
4. The controller display should now be indicating
the set point temperature. Make note of the ther-
mometer reading without opening the glass
door.
5. Press and hold both arrow keys until the con-
troller display indicates tunE. Release the
arrow keys. Press and release the down arrow
key, the display should now indicate LEUL.
Press and hold the “STAR” (✳) key and using
the UP arrow key adjust the display to read 3.
Release the “STAR” (✳) key. Press and release
the UP arrow key until the display indicates
Zero. The display should now alternate
between Zero and a numerical value.
OPERATION
12

6. Using the examples shown below and the ther-
mometer value obtained in the previous step,
enter the correct Zero value into the controller
by pressing the “STAR” (✳) key and using the
UP or DOWN arrow key. If there is already a
Zero value present then add the new value to
the one already present.
7. When the correct Zero value has been entered,
press and hold the two arrow keys together until
the display again indicates the chamber temper-
ature. If the procedure was done correctly, the
controller display should now agree with the
thermometer reading to within ±0.5ºC.
8. Allow the unit to run for at least 30 minutes.
9. Re-check the thermometer reading, the con-
troller display and the thermometer should agree
to within ±0.5ºC. If not repeat steps 4, 5
and 6 above.
OPERATION
Thermometer = 60°C
Controller Reading = 65°C
Subtract = -5°C
Enter Zero value of -5°C
Thermometer = 70°C
Controller Reading = 65°C
Subtract = +5°C
Enter Zero value of +5°C
13

The following is intended as a guide to help in servicing this unit, if problems should occur.
Troubleshooting
Note
Before attempting any repair, discon-
nect power cord from outlet.
Note
Aqualified controls service techni-
cian must perform maintenance and
repairs.
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14

Checking Controls
• While using the unit, occasionally check that both
control panel status lamps are functioning as
described in the previous OPERATION section.
• During normal operation, with controls properly set,
the over-temperature status lamp is not supposed to
be functioning. If it is and continues to be lit after re-
calibration as described in the previous OPERA-
TION section, follow the procedures described in the
upcoming TROUBLESHOOTING section to locate
the source of the problem.
Cleaning
• Clean any spills immediately. Use only soap or mild
detergent and water with a soft cloth for cleaning.
Do not use abrasives on the glass inner door. Refer
to following section for care of stainless steel sur-
faces.
• Wipe exterior with soft damp cloth as necessary to
remove dust, fingerprints or other smudges.
Care and Cleaning of Stainless Steel
WARNING: Electrolysis can damage stainless steel. This
occurs when an object is allowed to rest directly on the sur-
face of stainless steel, trapping moisture that becomes oxy-
gen-starved, but is surrounded by water containing oxygen.
The Alloy Called Stainless
Stainless steel is an alloy of steel with chromium and nickel
that increase the metal’s resistance to rust and corrosion.
Yet, if not properly cared for, stainless steel can rust and cor-
rode.
Exposure to air provides the passivation, or oxide layer coat-
ing, for clean stainless by producing a thin, durable chromi-
um-oxide film that forms rapidly on the alloy surface to give
Maintenance
Warning
Disconnect from the power supply
prior to maintenance and servicing.
Warning
Refer servicing to qualified personnel.
15

stainless its characteristic “stainless” quality. Also expo-
sure of the surface to other oxidizing environments can
produce a passivating film or coating.
However, if free oxygen is not available due to scale or
contamination buildup the metal surface may become vul-
nerable to rusting and corrosion as well as pitting. But by
maintaining neutral pH and conducting frequent cleanings
with detergent and water, years of trouble-free service
from stainless steel products can be obtained.
Stainless Guidelines
Distilled water is recommended. Please note, if this water
is very pure it may be corrosive to stainless. When filling
a bath or incubator, ALWAYS add 2 to 40 ppm (20 to 40
mg/liter) disodium phosphate or sodium bicarbonate,
adjusting dosage to provide a pH value of 7 to 9. If not
available, use clean, aerated soft tap water provided the
total solids concentration is < 500 PPM. We do NOT rec-
ommend using 18 meg-ohm deionized water. If this is the
only source of treated water available, mix with regular
tap water at a 50/50 ratio.
The pH Factor
Check pH regularly. If pH is <6.0, add disodium phos-
phate to increase pH to a 7 to 9 value. Sodium carbonate
or sodium bicarbonate may be used but they tend to form
scale that must be rinsed out regularly. If pH is >10.0,
add sodium bisulfate to decrease pH to a 7 to 9 value.
Avoid adding harsh alkalines or acids since these may
cause localized corrosion and result in unstable pH.
MAINTENANCE
16

MAINTENANCE
Note
The use and disposal of these
chemicals may be regulated by your
local city codes; consult those regu-
lations before disposing of these
materials.
Special Considerations
WARNING: If it is necessary to use the following chemi-
cals, limit exposure time to a maximum of 3 hours.
Always clean surfaces immediately after use.
BE ADVISED: Never use the following on stainless steel:
Aqua regia
Ferric chloride
Iodine
Sodium acid
Sodium azide
Chemical spills, especially those agents listed here,
should be removed as soon as possible and the stainless
steel surface cleaned with mild soapy water followed by a
clean water rinse.
Cleansing Agents
Anti-fungal and anti-bacterial additives are permissible to
use as long as the pH of the aqueous solution is kept
within the range of 7 to 9. These are available through
laboratory distributors. Be sure to CONFIRM that they are
not harmful to stainless steel.
Do not use any metallic pads. Instead, for stubborn
stains, use a plastic light-duty cleansing pad and rub gen-
tly in the direction of the metal grain.
If stains continue to persist, use one of the following
chemicals and methods.
Cleaning Methods
CAUTION: Extreme care must be taken when handling
these materials. Always work in an area with adequate
ventilation. Use the precautions as outlined in the Material
Safety Data Sheet (MSDS) and the manufacturer’s
instructions for the product being utilized. Also, follow the
17
Aluminum chloride E.D.T.A. Potassium permanganate
Barium chloride Ferrous chloride Potassium thiocyanate
Calcium chloride Lysol Sodium hypochlorite
Chlorinated Lime Mercury salts Stannous chloride
Citric acid (boiling) Phenol Tartaric acid
Dakin’s solution

Note
This information is intended as guide-
lines only and Barnstead International
makes no claim as to the suitability to
any particular situation. Consult your
staff chemist to determine what would
be best for your stainless steel prod-
uct and laboratory.
MAINTENANCE
personal protection index found in the hazardous materi-
als information system (HMIS) section of the MSDS.
•Any of a variety of “scale removers” available at
local supermarkets or hardware stores used for
the cleaning of coffee marks, humidifiers or
vaporizers.
• A 15% to 35% phosphoric acid solution avail-
able from laboratory supply distributors for scale
and rust removal. Allow solution to soak the
surface affected until rust and scale is loosened.
Immediately follow with a clean water rinse.
•Citric acid based cleaners.
•Bathroom tub and tile cleaners.
• A mixture of 20% nitric acid and 1.5% hydrofluo-
ric acid (or hydrochloric acid). Swab solution on
surface allowing it to remain until rust is loos-
ened. Immediately follow with a clean water
rise. This method should ONLY be used if
SEVERE rust and scale stains are present.
•Oxalic acid 2% to 5% in warm water. Swab
solution on surface allowing it to remain until
rust is loosened. Immediately follow with a clean
water rinse. This method should ONLY be used
if SEVERE rust and scale stains are present.
Regardless of the approach utilized, ALWAYS follow the
manufacturer’s directions and allow the chemicals to do
the cleaning with MINIMAL scrubbing. Always follow
cleanings with a clean water rinse. Air dry.
Materials Effective in Disinfecting
•Glutaraldehyde
•Alcohol
18

Servicing Guide
Heater Replacement
Heaters are not replaceable. Call Barnstead International
Customer Service at 1-800-553-0039 for assistance.
Thermostat Replacement
1. After unplugging the unit, remove the top cover of
the unit to reach control components. Expose 2
screws holding thermostat to the mounting bracket.
2. After removing thermostat from control panel, dis-
connect wiring while noting where each lead attach-
es.
3. Remove back cover of unit to reach thermostat bulb,
set in a bracket under the chamber floor.
4. Slide the bulb out of the bracket and through the top
hole to completely remove the thermostat.
5. Install new thermostat by reversing above proce-
dure.
Switch or Status Lamp
Replacement
1. Power switch and status lamps are snap-in mount-
ing type. Unplug unit, remove top cover and discon-
nect leads after noting where each attaches.
2. Push the switch out from the back. Slide the lamp
body sideways to remove lens.
3. Reverse procedure to install new lamp.
4. Press replacement switch in from the front of the
panel and then reconnect the leads as noted.
Switch is to be oriented so that the green lamp is
toward the top.
Warning
Disconnect from the power supply
prior to maintenance and servicing.
Warning
Refer servicing to qualified personnel.
19

SERVICING GUIDE
Note
PCB contacts at rear of controller fit
into contacts at rear of controller
housing.
Replacing Temperature
Controller
1. Place ON/OFF switch in OFF position.
2. Unplug incubator from outlet power supply.
3. To remove controller from control housing:
a) Use both hands to firmly grip each side of the
controller bezel.
b) Press on the bezel side grips until the bezel
tabs release.
c) Slowly pull controller from housing
4. To install new, factory-configured controller:
a) Carefully slide new controller into controller
housing.
b)Press controller bezel into controller housing
until bezel tabs securely lock controller into
place.
5. Plug incubator into outlet power supply.
6. Place ON/OFF switch in ON position.
20