Benchmark Scientific myBlock BSH5002 User manual

Operation Manual
BSH5002
myBlock™ Dry Bath
Incubator

Contents
CHAPTER 1 INTRODUCTION--------------------------------------------------------------------------------- 1
CHAPTER 2 SPECIFICATIONS------------------------------------------------------------------------------- 2
1. AMBIENT OPERATING ENVIRONMENT:------------------------------------------------------------ 2
2. TECHNICAL DATA:----------------------------------------------------------------------------------------- 2
CHAPTER 3 OVERVIEW---------------------------------------------------------------------------------------- 3
1. STRUCTURE OVERVIEW-------------------------------------------------------------------------------- 3
2. KEYPADS ----------------------------------------------------------------------------------------------------- 4
3. DISPLAY ------------------------------------------------------------------------------------------------------- 4
CHAPTER 4 OPERATION-------------------------------------------------------------------------------------- 5
1. TEMPERATURE AND TIME SETTING---------------------------------------------------------------- 5
3. TEMPERATURE CALIBRATION------------------------------------------------------------------------ 6
4．TEMPERATURE CONTROL BY EXTERNAL SENSOR------------------------------------------10
CHAPTER 5 FAILURE ANALYSIS AND TROUBLESHOOTING -----------------------------------11
ANNEX：WIRING DIAGRAM---------------------------------------------------------------------------------12

BSH5000 Operation manual Chapter 1 Introduction
─1─
Chapter 1 Introduction
The dry bath incubator is controlled by sophisticated software, which
can be widely used for the preservation and reaction of samples, the
amplification of DNA, the pre-denaturation of the electrophoresis, blood
serum coagulation and a variety of laboratory procedures.
Features:
Large digital display of time and temperature
Extremely accurate temperature control up to 105°C
Built-in over-temperature protection
In-Lab calibration
Plastic lid for enhanced precision and eliminating waste of energy
Quick-Flip blocks available for a variety of tube sizes

BSH5000 Operation manual Chapter 2 Specifications
—2 —
Chapter 2 Specifications
1. Ambient operating environment:
Ambient temperature：5°C ∼35°C
Relative humidity：≤70%
Power supply：AC115V or 230V, 50-60Hz
2. Technical Data:
Item No. BSH5000 (-E)
Temperature range Ambient (Room Temp) +5
°
C
∼
105
°
C
Timer Max. 99hr & 59min.
Temperature accuracy ≤
±
0.1
°
C
Temperature uniformity ≤
±
0.2
°
C
Heating time
（
20-105
°
C
）
≤20min
Block material Aluminum
Power 120W (single)/240W (dual)
Fuse 250V 4A Ф5×20
Dimensions (L×W×H) 7x10x5 in. / 18x25x13 cm (single)
6.3x14.5x5 in./ 16x36.5x13 cm (dual)
Warranty 2 Years

BSH5000 Operation manual Chapter 3 Overview
—3 —
Chapter 3 Overview
This chapter focuses on the introductions of the structure, keypads and key-functions of
the instrument, as well as preparatory work prior to initial operation.
1. Structure overview
Housing
Power inlet
Fuse
On/Off button
Transparent cover
Dual Block chamber
Control panel

BSH5000 Operation manual Chapter 3 Overview
—4 —
2. Keypads
3. Display


Start
Temp.
℃ Time
TM
StopStart
External
Temp.
℃ Time
TM
StopStart
External
Start key: After the temperature and time setting is selected,
press this key to start the timer.
Stop Key: Press this key to stop the timer
Down key: Used in both time and temperature mode to
decrease the setting.
Up key: Used in both the time and temperature mode to
increase the setting.
Stop

BSH5000 Operation manual Chapter 4 Operation Guide
—5 —
Chapter 4 Operation
1. Temperature and time setting
a) Press the On/Off switch to power on the
instrument. The instrument performs a self
test followed by an alarm to signal that the
product is ready for use.
b) After about 3 sec. the temperature will
automatically increase to the most recent
setting. The values shown on the display are
actual settings (real time temperature & time
remaining.) The example shows an actual
temp. of 28.5°C and 35 min. remaining.
c) To adjust the set temperature, use the and 
keys to select the desired temperature (Ex.
55.5). After 5 seconds, the temperature is
stored and the instrumnent will begin heating.
d) To set the time, press or keys to set the
desired time (ex. 1 hr 20 min.). After 5
seconds the desired time is stored.
NOTE: The timer will not begin unless the
“Start/Stop” button has been pressed

BSH5000 Operation manual Chapter 4 Operation Guide
—6 —
2. Timer Operation (Advanced)
The default setting of the instrument is designed so that the heater will continue to
hold the set temperature even once time has expired and “oVEr” is showing on the
time display. This setting can be adjusted so that the heater will shut down once time
has expired. To adjust the advanced time setting, set the “Stop” key and hold it for 10
seconds. After 10 seconds, the display will show “OP:1” use the or key to select
“OP:2”. The setting has now been changed and can be stored by pressing the
“Start” key.
3. Temperature Calibration
The temperature of the instrument has been calibrated prior to shipment. However, in
the event that an adjustemnt to the calibration is required, the user can adjust the
calibration with either a thermometer/thermocouple or with the optional external
temperature probe. (sold separately).
Caution: To ensure accuracy over a wide range of temperatures, the instrument is
calibrated at two temperatures points, both 40°C and 100°C.
a) Calibration with a thermometer:
a) Power on the machine and ensure that the temperature on the display is less than
35°C.
b) Inject olefin or mineral oil into one of the block wells, and then put a
thermometer/thermocouple into this well (make sure that the precision of the
thermometer should be within 0.1°C and it be fully submerged into the well). See
Fig a.

BSH5000 Operation manual Chapter 4 Operation Guide
—7 —
c) Press “Stop” key and the key simultaneously for 3 seconds,
the bath enters into the temperature calibration interface.
At this point, “ ” is displayed, indicating entry
into temperature calibration mode. The temperature
displayed is current measured temperature and over time,
it begins to rise to 40.0 C. (Note, it may take up to 30 minutes
before a change on the display to 40°C is shown.)
Once held constant at 40.0 °C, the decimal digit begins to flash.
Please wait 20 minutes, then the user can read the value on the
Thermometer and use the up and down keys to match the temperature on the
display to the value shown on the thermometer.
Note: It is important to allow 20 minutes for the temperature to
equilibrate before making any adjustment to the temperature.
Ex. If the actual read out of thermometer is 39.6, modify the
temperature to 39.6 by pressing or . Then press “Start”
to confirm the input value.
The 40° Calibration has now been completed and the dry bath
begins the 100°C calibration
Fig. a
Thermometer
Olefin oil
Cone-shaped well

BSH5000 Operation manual Chapter 4 Operation Guide
—8 —
d) Once the “Start” button is pressed, Then the instruments will the automatically
begin to heat to 100°C. Once 100°C is shown on the display (this may take up to 30
minutes) and held as constact, the decimal begins to flash. Please wait 20 minutes
and then check the actual value from thermometer.
Note: It is important to allow 20 minutes for the temperature to
equilibrate before making any adjustment to the temperature.
If the actual thermometer shows 101.5, modify the temperature display
to 101.5 by pressing or . Then press “Start” to confirm
the input value.
Note: Pressing “Stop” and “” simultaneously during the temperature calibration
indicates exiting the temperature calibration program. The adjustments will be
cancelled and the changes will not take effect.
3.2. Calibration with the External Temperature Probe:
a) Power on the machine. Before beginning the calibration, always ensure that the
temperature on the display is less than 35°C.
b) Put External Temperature Probe into a block well.
c) Press “Stop” key and the “” key simultaneously
and hold for 3 seconds. This will enter the temperature
calibration mode with the external probe. “ ”
will be displayed, indicating entry into external temperature
calibration mode. The temperature displayed is the External
probes current temperature and it will begin to rise to 40.0
automatically. (Note, it may take up to 30 minutes
before a change on the display to 40.0°C is shown.)

BSH5000 Operation manual Chapter 4 Operation Guide
—9 —
Once held constant at 40.0 °C, the decimal digit begins to flash.
Note: It is important to allow 20 minutes for the temperature to
equilibrate before making any adjustment to the temperature.
After 20 minutes, Press the “Stop” key to display the temperature
value of External Probe. (Ex. If the displayed value is 39.6°C.)
Press “Stop” again (or wait for 6 seconds), then display shows
“
” . The temperature can now be adjusted by
pressing or to match the measured value (ex. 39.6).
Then press the “Start” key to confirm.
The 40° Calibration has now been completed and the dry bath
begins the 100°C calibration
d) The instrument will thenheat to 100.0°C automatically. Once 100.0°C has been
reached and held constant, the decimal point will begin to flash.
Note: It is important to allow 20 minutes for the temperature to
equilibrate before making any adjustment to the temperature.
After 20 minutes, press the “Stop” key to show the actual
readout of the external probe (for ex. 101.5°C).
Press “Stop” again (or wait for 6 seconds), then display shows
“
” . Now adjust the displayed temperature to the
measured value (101.5) by pressing or . Then press the “Start” key to confirm.
Note: Pressing “Stop” and “” simultaneously during the temperature calibration
indicates exiting the temperature calibration program. The procedure will be
cancelled and the changes will have of no effect.

BSH5000 Operation manual Chapter 4 Operation Guide
—10 —
4．Temperature control by external sensor
a) Insert the external temperature probe into a well of the dry bath or directly into a
sample tube.
b) Press and hold “Stop”, then simultaneously press and hold “” for 3 seconds, the
external indicator light goes on to indicate that it has entered into the temperature
control mode of external sensor.
Note: After entering into the external temperature control mode, the user can
simultaneously press “Stop” and "" for 3 seconds to exit this mode. Upon
exiting the external indicator light will go off.

BSH5000 Operation manual Chapter 5 Failure Analysis and Troubleshooting
—11 —
Chapter 5 Failure Analysis and Troubleshooting
Problems and actions
No.
Common problem
Possible cause
Action(s)
1
No display on the
screen
No power on the main
power plug
Check power supply and
plugged properly
Faulty fuse Change fuse
On/Off button broken Change button
Others Contact the supplier
2
The actual and
displayed temperatures
are quite different
Broken sensor or
loose contact of the
block Contact the supplier
3
“OPEn” displayed,
alarming “beep” Sensor disconnect
Contact the supplier
“SHOr” displayed,
alarming “beep” Sensor short-circuit
“HHHH” displayed,
alarming “beep”
Sensor broken, or
block temperature is
too high
4 No heating Sensor broken Contact the supplier
Heating tube broken
5 Keys don’t work Faulty key Contact the supplier

BSH5000 Operation manual Annex
—12 —
Annex：Wiring Diagram
（ for reference only ）
L
PE
NSwitch
USB PT1000
CONN BOARD
J3 J2
J1 AC220V
J4 Heat
POWER
BOARD
J3HeatCtl J2 AC9V
J2 PT100 J4 PT1000USB1 USB
J1 AC9V
X1 HeatCtl
MAIN
BOARD
HEAT
PT100
Block

Memo