BioCision CoolCell LX User manual

CoolCell® LX Instructions for Use CoolCell® LX Instructions for Use
Controlled-rate cell freezing container for 1ml or 2ml cryovials Controlled-rate cell freezing container for 1ml or 2ml cryovials
Quick Start
• The 12 chambers and cryovials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1 ml of cell suspension in each well. Each well should contain a filled vial. If
freezing batch is fewer than 12 vials, fill each empty well with a BioCision CoolCell Filler Vial (2ml vial, part
number BCS-3105) or other vial that contains equivalent volume of freezing media.
Note: Cell suspensions can be inserted into a room temperature CoolCell and successfully preserved. For optimal
results, CoolCell should be at the same temperature as your cell suspensions.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell LX
• Place CoolCell LX upright into a -80OC freezer or dry ice locker. Ensure that there is at least one inch of
free space clearance around CoolCell LX module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell LX from the freezer and gently remove the lid using a gentle twisting and rocking motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer cryovials containing cells to permanent storage to avoid temperature rise
and cell damage. Cryovial contents can rise from -8oOC to over -50OC in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell LX to room temperature
CoolCell LX is ready to freeze again as soon as the foam body and core (black ring) are at room temperature.
To rapidly recycle CoolCell LX to room temperature, remove the center solid core ring. CoolCell LX body and
lid will return to room temperature in 10 to 15 minutes. Check that all chambers are dry. Dry the core ring
before re-inserting into the central chamber.
Quick Start
• The 12 chambers and cryovials should be dry to avoid tube sticking upon freezing.
• Make sure the core (black ring) is at room temperature and seated in the bottom of the central cavity.
• Place sample vials containing 1 ml of cell suspension in each well. Each well should contain a filled vial. If
freezing batch is fewer than 12 vials, fill each empty well with a BioCision CoolCell Filler Vial (2ml vial, part
number BCS-3105) or other vial that contains equivalent volume of freezing media.
Note: Cell suspensions can be inserted into a room temperature CoolCell and successfully preserved. For optimal
results, CoolCell should be at the same temperature as your cell suspensions.
• Check that the tubes slide in and out freely.
• Fully seat the lid on CoolCell LX
• Place CoolCell LX upright into a -80OC freezer or dry ice locker. Ensure that there is at least one inch of
free space clearance around CoolCell LX module.
• Freeze for four hours before transferring samples to archive storage.
Transferring frozen samples to archive storage
• Prepare an insulated pan or container with a one inch (2.5cm) layer of pulverized or pellet dry ice.
• Remove CoolCell LX from the freezer and gently remove the lid using a gentle twisting and rocking motion.
• Vial tops will be exposed once lid is removed and vials should be quickly extracted and placed onto the
dry ice.
Special Notes:
• Always use dry ice to transfer cryovials containing cells to permanent storage to avoid temperature rise
and cell damage. Cryovial contents can rise from -8oOC to over -50OC in less than one minute if exposed to
room temperature air.
• It is strongly recommended that all frozen cell cultures be checked for viability before the stock culture is
terminated.
Recycling CoolCell LX to room temperature
CoolCell LX is ready to freeze again as soon as the foam body and core (black ring) are at room temperature.
To rapidly recycle CoolCell LX to room temperature, remove the center solid core ring. CoolCell LX body and
lid will return to room temperature in 10 to 15 minutes. Check that all chambers are dry. Dry the core ring
before re-inserting into the central chamber.

About CoolCell LX
CoolCell LX, in combination with a -80OC freezer or dry ice locker, will provide the freezing rate of -1OC per min-
ute that is ideal for cryo-preservation of most cultured cell lines. CoolCell LX design uses a combination of
insulation foam, radial symmetry, and a heat transfer core to regulate heat loss rather than using a large thermal
mass (alcohol-based freezing container). As a result, freezing profiles are extremely consistent from one run to
the next. Also, because of this low thermal mass, CoolCell LX will not cause a rise in local freezer temperature
and will protect nearby samples already stored in the freezer. Low thermal mass also means CoolCell LX will
rapidly return to room temperature for another freezing cycle (see fast recycle instructions above).
CoolCell LX freezing performance
A temperature probe was placed into a 2ml cryovial con-
taining 1ml of cryopreservative and the tube was inserted
into a CoolCell LX sitting at room temperature. CoolCell
LX was then placed directly into a -80°C freezer and
the temperature rate and profile were observed over a 3
hour period. This experiment was repeated 5 consecutive
times and temperature profiles were recorded.
Conclusion: CoolCell LX showed identical cooling profiles
and phase transition over five consecutive freeze cycles.
Troubleshooting
Care and cleaning
CoolCell LX is constructed of closed cell cross-linked polyethylene foam and a solid thermo-conductive core.
CoolCell LX is compatible with prolonged cryogenic temperature exposure. The foam may be cleaned by water
and mild soap. Rinse and dry thoroughly. CoolCell LX is resistant to alcohols and 10% bleach solutions. Do not
autoclave. Maximum temperature exposure: 60OC. Avoid prolonged exposure to UV light sources.
CoolCell®LX2ThermalProfile
050 100 150 200
-80
-60
-40
-20
0
20
Time in Minutes
Temperature
o
C
CCLX-5
CCLX-1
CCLX-2
CCLX-3
CCLX-4
Problem Solution
Vials do not freely fit in the
chambers
CoolCell LX is designed to fit standard screw-top 1ml and 2ml cryovials
up to 13mm in diameter and up to 55mm in height. Check that flag style
labels, if used, will not bind and hinder insertion or removal.
Vials are stuck in CoolCell after
freezing
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell LX to
dislodge vials.
The lid does not fully seat Ensure that sample tubes are fully seated in chamber. The maximum
height of the tube is 55mm.
BioCision, LLC
775 E. Blithedale, Suite 203, Mill Valley, CA 94941, USA, [email protected], www.biocision.com
CoolCell LX Instructions, October 2012
CoolCell LX © 2012. BioCision, LLC. All rights reserved. Patents pending. BioCision, Standardizing Samples, CoolCell designations are
trademarks owned by BioCision, LLC.
PURCHASE AND/OR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THE
TERMS AND CONDITIONS OF THIS RESEARCH LICENSE AGREEMENT. The purchase of this product (“the Product”) conveys to the buyer
only the non-transferable right to use the product in internal non-commercial research (“Research Use”) conducted by the buyer. Research Use does not include the right to
use the Product to manufacture products, including but not limited to cells or other biological materials, for subsequent sale; the use the Products to discover, develop, or test
any commercial product; and the re-sale of Products, either alone or in combination with other products. The sale or any other transfer or distribution to third parties of (i)
the Product, or (ii) any cells or other materials created using the Product is strictly prohibited under this Research License Agreement. Also, the use of cells or other materials
created using the Product to discover, develop, or test any commercial product is strictly prohibited under this Research License Agreement. A commercial license is required
for any commercial use of the Product, or any commercial use of cells or other materials discovered, developed, or tested using the Product. To obtain a commercial license
to use the Product, please contact BioCision.The buyer’s rights to have and use the Product under the Research License Agreement will terminate immediately if the buyer en-
gages in commercial use of the Product. Upon such termination of the buyer’s rights, the buyer agrees to return the Products to BioCision. This Research License Agreement
shall not be assigned or otherwise transferred by the buyer.
About CoolCell LX
CoolCell LX, in combination with a -80OC freezer or dry ice locker, will provide the freezing rate of -1OC per min-
ute that is ideal for cryo-preservation of most cultured cell lines. CoolCell LX design uses a combination of
insulation foam, radial symmetry, and a heat transfer core to regulate heat loss rather than using a large thermal
mass (alcohol-based freezing container). As a result, freezing profiles are extremely consistent from one run to
the next. Also, because of this low thermal mass, CoolCell LX will not cause a rise in local freezer temperature
and will protect nearby samples already stored in the freezer. Low thermal mass also means CoolCell LX will
rapidly return to room temperature for another freezing cycle (see fast recycle instructions above).
CoolCell LX freezing performance
A temperature probe was placed into a 2ml cryovial con-
taining 1ml of cryopreservative and the tube was inserted
into a CoolCell LX sitting at room temperature. CoolCell
LX was then placed directly into a -80°C freezer and
the temperature rate and profile were observed over a 3
hour period. This experiment was repeated 5 consecutive
times and temperature profiles were recorded.
Conclusion: CoolCell LX showed identical cooling profiles
and phase transition over five consecutive freeze cycles.
Troubleshooting
Care and cleaning
CoolCell LX is constructed of closed cell cross-linked polyethylene foam and a solid thermo-conductive core.
CoolCell LX is compatible with prolonged cryogenic temperature exposure. The foam may be cleaned by water
and mild soap. Rinse and dry thoroughly. CoolCell LX is resistant to alcohols and 10% bleach solutions. Do not
autoclave. Maximum temperature exposure: 60OC. Avoid prolonged exposure to UV light sources.
CoolCell®LX2ThermalProfile
050 100 150 200
-80
-60
-40
-20
0
20
Time in Minutes
Temperature
o
C
CCLX-5
CCLX-1
CCLX-2
CCLX-3
CCLX-4
Problem Solution
Vials do not freely fit in the
chambers
CoolCell LX is designed to fit standard screw-top 1ml and 2ml cryovials
up to 13mm in diameter and up to 55mm in height. Check that flag style
labels, if used, will not bind and hinder insertion or removal.
Vials are stuck in CoolCell after
freezing
It is likely moisture was in the vial chambers or on the sample vial prior
to freezing. Remove the core (black ring) and tap the CoolCell LX to
dislodge vials.
The lid does not fully seat Ensure that sample tubes are fully seated in chamber. The maximum
height of the tube ist 55mm.
BioCision, LLC
775 E. Blithedale, Suite 203, Mill Valley, CA 94941, USA, [email protected], www.biocision.com
CoolCell LX Instructions, October 2012
CoolCell LX © 2012. BioCision, LLC. All rights reserved. Patents pending. BioCision, Standardizing Samples, CoolCell designations are
trademarks owned by BioCision, LLC.
PURCHASE AND/OR USE OF THIS PRODUCT SHALL CONSTITUTE ACKNOWLEDGMENT AND ACCEPTANCE OF THE
TERMS AND CONDITIONS OF THIS RESEARCH LICENSE AGREEMENT. The purchase of this product (“the Product”) conveys to the buyer
only the non-transferable right to use the product in internal non-commercial research (“Research Use”) conducted by the buyer. Research Use does not include the right to
use the Product to manufacture products, including but not limited to cells or other biological materials, for subsequent sale; the use the Products to discover, develop, or test
any commercial product; and the re-sale of Products, either alone or in combination with other products. The sale or any other transfer or distribution to third parties of (i)
the Product, or (ii) any cells or other materials created using the Product is strictly prohibited under this Research License Agreement. Also, the use of cells or other materials
created using the Product to discover, develop, or test any commercial product is strictly prohibited under this Research License Agreement. A commercial license is required
for any commercial use of the Product, or any commercial use of cells or other materials discovered, developed, or tested using the Product. To obtain a commercial license
to use the Product, please contact BioCision.The buyer’s rights to have and use the Product under the Research License Agreement will terminate immediately if the buyer en-
gages in commercial use of the Product. Upon such termination of the buyer’s rights, the buyer agrees to return the Products to BioCision. This Research License Agreement
shall not be assigned or otherwise transferred by the buyer.