Bioneer Exiprep 96 Lite User manual

ExiPrep™96 Lite
Automated NAPurification System
35℃
15℃
MT Promedt Consulting GmbH Altenhofstr. 80
D-66386 St. Ingbert. Germany. Tel [phone]
A-5250

ExiPrep ™96 Lite
ExiPrep™96 Lite
Automated NAPurification System
User’s Guide
Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon
34302, Republic of Korea
Tel: [phone]
Fax: [phone]
Email: [email protected]
www.bioneer.com

ExiPrep ™96 Lite
Intended Use
It is instrument capable of extracting nucleic acid needed in various samples as blood, serum, sputum and
tissues for the gene amplification test.
Warranty and Liability
All Bioneer products undergo extensive Quality Control testing and validation. Bioneer guarantees quality
during the warranty period as specified, when following the appropriate protocol as supplied with the product.
It is the responsibility of the purchaser to determine the suitability of the product for its particular use. Liability
is conditional upon the customer providing full details of the problem to Bioneer within 30 days.
Trademark
ExiPrep ™ is a trademark of Bioneer Corporation.
Copyright
Copyright 2018. Bioneer Corporation. All Rights Reserved
Notice
Bioneer corporation reserves the right to make corrections, modifications, improvements and other changes
to its products, services, specifications or product descriptions at any time without notice. All information
provided here is subject to change without notice.

ExiPrep ™96 Lite
U.S. and Canadian Safety Standards
Standard for Electrical instrument for measurement, control and laboratory use;
▪Part1: General Requirements,
UL 61010-1, 2nd Ed, Rev., October 28, 2008&CAN/CSA-C22. 2 No. 61010-1-04(R2009)
▪Part 2: Particular Requirements for Automatic and Semi-Automatic Laboratory Instrument for Analysis
and Other Purposes,
CAN/CSA-C22. 2 No. 61010-2-081:04
▪Part 2-010: Particular Requirements for Laboratory Instrument for the Heating of Materials
CAN/CSA-C22.2 NO. 61010-2-010-04
▪Part 2: Particular requirements for in vitro diagnostic (IVD) medical instrument,
CAN/CSA-C22. 2 No. 61010-2-101:04
MANUFACTURER
: Bioneer Corporation
8-11, Munpyeongseo-ro, Daedeok-gu, Daejeon, 34302
Republic of Korea
EUROPEAN
REPRESENTATIVE
: MT Promedt Consulting GmbH
Altenhofstr. 80
D-66386 St. Ingbert, Germany
Phone: [phone]
PRODUCT
: ExiPrep ™96 Lite
Automated NA Purification System
CATALOG NO.
A-5250

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Contents
I. Overview ..................................................................................................................... 1
II. Symbols description ....................................................................................................... 2
III. Safety warnings and cautions ............................................................................................ 5
IV. Precautions on waste liquid............................................................................................... 8
V. Product configuration and description................................................................................... 9
1. Product configuration .................................................................................................... 9
2. Specifications ........................................................................................................... 10
3. Instrument structure and name ...................................................................................... 11
VI. Product installation requirement......................................................................................... 15
VII. Removing the shipping bracket and approving power source....................................................... 17
1. Removing shipping bracket ........................................................................................... 17
2. Approving power source............................................................................................... 18
3. Changes of Magnetic Rods ........................................................................................... 19
VIII. Magnetic Rod Covers and Cartridges Preparation .................................................................... 21
IX. Software Operation ....................................................................................................... 24
1. How to use the software .............................................................................................. 24
2. UV Lamp ................................................................................................................. 28
3. Setup –General ......................................................................................................... 28
4. Setup –Log .............................................................................................................. 29
5. Setup –Device .......................................................................................................... 30
X. Troubleshooting ........................................................................................................... 31
XI. User Maintenance ......................................................................................................... 33
1. Cleaning of the main body............................................................................................ 33
2. Replacing the Fuse ..................................................................................................... 33
3. Storage Method ......................................................................................................... 35
4. Shipping the Instrument for Service ................................................................................. 35
XII. Warranty .................................................................................................................... 36

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I. Overview
This is the instrument that can extract nucleic acid. In the case of nucleic acid extraction, entire process from
sample lysis to elution can be done in a short time.
In addition, it can refine fluidly per individual sample unit and has an automated dispensing function to increase
efficiency and productivity, saving time and cost. This unit comprises a syringe unit, heating block and a
cooling system.
To increase nucleic acid extraction efficiency, the ExiPrep ™96 Lite has a heating block. Sample heating
reduces experiment time and increases elution efficiency, resulting in increased sample yield.
The responsive 7 inch touch-screen enables simple instrument operation. From selecting sample-optimized
protocols to turning the UV sterilization lamp on and off, the touch-screen is designed for optimal workflow.
During instrument operation, the touch-screen will give real-time feedback on the progress of the extraction,
maximizing user convenience.
ExiPrep ™96 Lite comes with a contamination shield designed to protect the assay from cross-contamination
during operation. During the cartridges are in motion, the contamination shield will automatically slide upper the
cartridges. This will eliminate the possibility of intra-assay cross-contamination, which is critical when working
with multiple samples.

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II. Symbols description
Safety Labels Description on the ExiPrep ™96 Lite
English
Be cautions when moving the door, You will get hurt when it opens and shuts.
Francais
Faire attention lors du déplacement de la porte. Risque de blessures corporelles lors
de l’ouverture et de la fermeture.
English
Do NOT open the door during instrument’s operation. You can be injured your hands
or body.
Francais
Ne pas ouvrir la porte pendant la durée des opérations des instruments.
Risque de blessures corporelles.
Symbols for safe use
The table below presents the symbols used in user guide.
Conformité Européenne Mark
In vitro diagnostic medical instrument
Temperature limit
Manufacturer
Authorized representative in the European Community
Catalog number
Please refer to ‘Safety precautions’ contained in the manual.
Symbols for safety
Hot surface.
Note that surface is hot.
When Instrument is moving. You can be injured your fingers or hands.
Do NOT open the door during instrument’s operation.
Injuries many result when opening and closing door.
Be cautions when moving the door, You will get hurt when it opens and
shuts.
Please note that there is a risk of infection if you contact the waste liquid
inside a instrument.
Electrical Symbols

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Indicates the On position of the main power switch.
Indicates the Off position of the main power switch.
Indicates the standby switch status while the main power source is ‘On’.
Displays the ground terminal for the main protective ground of the
instrument.
Indicates a terminal that receives or supplies alternating current or voltage.
Indicates a terminal that receives or supplies direct current or voltage.

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Environmental Symbols on the ExiPrep ™96 Lite
The following symbol (WEEE) applies to the ExiPrep ™96 Lite placed on the European market.
Do not dispose of ExiPrep ™96 Lite as unsorted municipal waste.
Follow local municipal waste ordinances for proper disposal provisions to
reduce the environmental impact of waste electrical and electronic
instrument.
European Union customers:
Call your local Europe office for Bioneer instruments pick-up and recycling.

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III. Safety warnings and cautions
Please read carefully warnings and cautions for safety before use. The precautions listed herewith are
important safety messages and should be observed to ensure the product is used safely and properly to
prevent accidents and danger. In this manual, the degree of risk from improper handling is classified into
the following three categories. “Safety warnings and cautions” is classified into “Danger”, “Warning” and
“Caution” and they mean the following:
Ignoring this sign or improper handling may result in death or serious injury.
Hazards or dangerous actions that may result in minor injury or damage.
Hazards or dangerous actions that may cause a burn.
Hazards or dangerous actions that may result in electric shock.
1. Cautions for experimenters or during the experiment
1) Be sure that the power cord (100-240V~, 50/60Hz) is properly connected to the main unit and that the
power cord is plugged in the outlet correctly. If the power cord is not properly connected the power
many not turn on and cause malfunction.
2) This is a instrument to extract a nucleic acid. Please use it according to the purpose.
3) Operating the LCD touch screen during operation of this product may result in stoppage or error in the
test results. Be sure to operation the LCD touch screen after the operation of instrument is completed.
4) Do not turn off the power or shut down the program during operation. Data communication errors can
cause the instrument to operate unsteadily or affect the results of the experiment.
5) Handling the product with wet hands may cause malfunction or electric shock. The power cord should
be handled with a dry hand.
6) When installing the cartridge, be sure to install completely close contact with the heat block that is
installed in the position of the cartridge. If it is not close contact, the performance of Prep can
deteriorate for the cartridge does not come in close contact with the heat block.
2. Environmental precautions
1) Do not use when the power plug is loose. The power plug damaged from overheating may cause fire
or electric shock.
2) Do not connect multiple instruments to a single outlet. Failure to do so may result in overheating of the
socket due to overload and may cause fire.
3) Be sure to handle the power cord with a dry hand when connecting and disconnecting. It may cause
electric shock.
4) Do not place objects near the instrument that may interfere with product operation.
5) Do not install the instrument in a dusty area. It may cause malfunction.
6) Install the instrument away from electric heating devices. It may cause fire.
7) Install the instrument avoiding areas of splashing water or humidity. It may cause electric shock, fire or
malfunction.

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8) Do not install the instrument in a place where flammable or corrosive gas is generated. If gas leaks,
do not operate the power cord, but open the window to ventilate. Fire sparks may cause explosion and
a fire.
9) Do not disassemble or modify the instrument. It may cause fire, electric shock or malfunction and may
not be covered by warranty.
3. Installation precautions
1) The instrument is a precision drive unit and should be installed away from direct sunlight.
2) Please install it on a flat surface where there is no vibration.
3) When installing the instrument, place the system at least 15cm away from the wall.
4) When installing the instrument, make sure that the cooling fan on the bottom is not damaged.
5) When connecting computer, router and ExiPrep ™96 Lite, please be sure the power is turned off.
Connecting the instrument while the power is on may damage the instrument.
6) When connecting computer, router and ExiPrep ™96 Lite, check the communication cable connection.
If the connection is unstable, the data may not be obtained due to the data transmission error while the
instrument is in operation.
4. Usage precautions
1) Wipe off the dust from the power plug and plug it securely so that the plug does not move. Unstable
connection may cause fire.
2) The ambient temperature of the instrument shall be within 15℃~35℃. If the external temperature is
too high, it may affect the operation of the instrument and accurate experimental results may not be
obtained.
3) Keep the recommended humidity (20~80%, no condensation). Excessive humidity or dryness may
cause corrosion or malfunction.
4) Do not place objects around sides and the rear of the instrument. It may cause malfunction during
operation.
5) This instrument is built with precision drive parts. Do not drop or give a strong shock. It may cause
malfunction and cause safety problems.
6) Turn off the power and unplug if not used for a long time. It may cause fire due to heating and ignition.
7) Be sure that the cooling fan on the bottom is not damaged. If the cooling fan does not work, it may
cause fire from overheating of the cooling fan motor and cooling agent.
5. Warnings and cautions and danger regarding the use and management of the product
1) This instrument shall be used only for nucleic acid extraction and shall not be used other than the
purpose described in the manual.
2) Be sure to use the accessories and cartridges supplied by us.
3) Do not modify or delete the information of the built-in instruments.
4) Do not touch the LCD touch screen with sharp objects. Using sharp drills, nails, picks, etc. may cause
damage the product.

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5) Please be careful when using solvent or strong detergent or chemical solvent on the surface of the
instrument. It may discolor. If those come into contact with the product, immediately wipe it gently off
with a soft cloth.
6) Do not store in high humidity for a long time. It will then be classified as malfunction due to immersion
and will not be covered under warranty. On the other hand, the product may not be used for they are
unrepairable even if you pay.
7) Modifying and disassembling the instrument may exclude you from warranty and not covered by after-
sale service.
8) Do not forcefully disconnect the power cord from the instrument during operation. It may cause
malfunction.
9) If you smell burning or experience severe overheating while using the product, stop using it
immediately and contact us.
10) Do not drop or give a strong shock. It may cause malfunction and may not be covered under warranty
regardless of a warranty period.
11) Be sure not to allow any foreign objects into the cooling fan on the bottom. If the cooling fan does not
work due to foreign objects, it may cause fire from overheating of motor and cooling agent.
6. Precautions in using sterilization(UV) lamp
1) The sterilization(UV) lamp automatically shuts off when the door is opened while it is in operation. For
safety, be careful not to have your eye or skin to come in contact while operating the sterilization lamp.
2) The sterilization(UV) lamp only works when the door is closed. Make sure that no foreign objects are in
the door sensor.
3) The product is programmed to operate for 15 minutes for sterilization and safety. Do not use the
sterilization for a long time.
4) Ultraviolet rays of the sterilization lamp may cause severe damage to eyes and skin and therefore not
advisable to see directly or indirectly. Be sure to take safety precautions such as ultraviolet shielding
instrument so as to not to see directly or indirectly or irradiate skin.

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IV. Precautions on waste liquid
This instrument uses a kit containing a solution comprised of a large amount of chlorine compound. Nucleic
acids extracted through the instrument and kits are conservative and may affect the performance if residues are
present. Please refer to the following for proper prevention of hazards.
1. Precautions immediately after using the Instrument
1) Waste liquid from the cartridges may overflow and contaminate the instrument if the tray is handled
abruptly resulting in false positive from the next experiment.
2) Waste liquid contains a large amount of chlorine ions that corrodes metals as stainless steels which
causes the instrument corrosion.
3) If the waste liquid contaminates inside the instrument due to mishandling of the cartridges, the
instrument must be disassembled and to wash clean. Please contact us.
2. Cleaning solution used for inside and outside of the Instrument
1) Use distilled water (DW), 70% ethanol, nucleic acid decomposition solution (5% Nitric acid, 1% lox,
DNAzap). Be careful for lox and DNAzap corrode metal. Moisten lint free cloth or paper towel, not
soaking wet to drip for it may drop on the instrument. Do not spray the liquid on the instrument.
3. Contamination of Instrument
If the instrument is contaminated, proper measures must be taken immediately to prevent accumulation
and damage.
1) If contamination occurs inside the instrument but did not yet make it into the inside, absorb it
immediately with a paper towel moisten with nucleic acid decomposition liquid and rinse with a paper
towel moisten with DW again. Absorb the remaining solution with a dry paper towel and operate UV
lamp to remove the contaminants from the instrument.
2) If contaminants enter inside the instrument, the instrument must be disassembled to wash. Please
contact us.
4. Waste Treatment
1) Waste liquid and consumables used must be disposed of properly according to the waste disposal
regulations.
2) Wear appropriate eyewear, clothing, and gloves when handling reagent and waste liquid.

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V. Product configuration and description
1. Product configuration
Part Name
Cat. No.
Qty.
ExiPrep ™96 Lite
A-5250
1 ea
32 well Magnetic Rods
3 ea
User’s Guide
1 ea
Power cord
1 ea
(Options) 8 well Magnetic Rods
3 ea

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2. Specifications
Physical specifications
Dimensions
40cm(W) x 57.5cm(D) x 54.6cm(H)
15.75in(W) x 22.64in(D) x 21.5in(H)
Weight
46 Kg (101.42 lbs)
Voltage / Frequency
100-240V~, 50/60Hz
Power
500VA Max (Fuse: 250V, F6.3AL, 2ea)
Operating specifications
Heating block
30-90℃ (86-194 ℉)
Temperature controlled block
4-90℃ (39.2 ~ 194 ℉)
Operating temperature
15 –35°C (59.0 –95.0℉)
Operating humidity
20 - 80%, no condensation
Operating system
Built-in
Communication
TCP/IP
User interface Display
7 inch Touch
Data Storage
USB 2.0 (x2)

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3. Instrument structure and name
(1) Outside
<Outside>
(2) Inside
<Inside 1>

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<Inside 2>
<Inside 3>

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(3) Buffer Cartridges
<Buffer Cartridges Layer 1>
<Buffer Cartridges Layer 2>

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<Buffer Cartridges Layer 3>

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VI. Product installation requirement
▪ ExiPrep ™96 Lite designed for indoor user and the following things must be considered in selecting the
place of installation.
-Satisfies the requirements for space and weight.
-Satisfies environmental requirements.
-The power outlet with the power rating of 550VA or above should be located within 1.5m (4.92ft.).
-Must be isolated from any form of moisture.
▪Materials needed
Box cutter, scissors, knifes, gloves
▪Unpack
1) Unpack ExiPrep ™96 Lite: