Bruker Best User manual

Version

BEST

Barcode Reader

Installation Manual

004

The information in this manual may be altered without notice.

BRUKER BIOSPIN accepts no responsibility for actions taken

as a result of use of this manual. BRUKER BIOSPIN accepts

no liability for any mistakes contained in the manual, leading to

coincidental damage, whether during installation or operation of

the instrument. Unauthorized reproduction of manual contents,

without written permission from the publishers, or translation

into another language, either in full or in part, is forbidden.

This manual was written by

Volker Reiß

Rheinstetten, Germany

P/N: Z31545

DWG-Nr.: 1252004

Installation Manual Version 004 iii

Contents

Contents .............................................................. iii

1 Introduction ............................................................ 5

1.1 General ............................................................................... 5

2 Adjusting the Z-Tower Height ................................ 7

2.1 General ............................................................................... 7

2.2 Hardware ............................................................................ 7

2.3 Software .............................................................................. 8

3 Mounting the Barcode Reader................................ 9

3.1 Mounting the Barcode Reader on the Gilson Liquid Handler . 9

3.2 Connecting the Power Supply ............................................ 12

3.3 Connecting the Serial Cable .............................................. 12

4 Software................................................................ 13

4.1 General ............................................................................. 13

4.2 Defining the Barcode Reader ............................................. 13

4.3 Programming the Barcode Reader ..................................... 15

5 Barcodes............................................................... 17

5.1 General ............................................................................. 17

5.2 Label Size ......................................................................... 17

5.3 General Barcode Information ............................................. 17

5.4 Matrix ID ........................................................................... 18

5.5 Matrix Type ....................................................................... 18

5.6 Barcode Positioning ........................................................... 20

5.7 Examples .......................................................................... 22

Figures ................................................................ 25

Tables .................................................................. 27

iv Installation Manual Version 004

Contents

Installation Manual Version 004 5 (29)

Introduction 1

General 1.1

This manual will guide you through the installation of the barcode reader for the

Gilson Liquid Handler.

The installation process consists of three steps:

1. Changing the height of the Gilson Z-tower.

2. Mounting the barcode reader on the Gilson Liquid Handler.

3. Defining the barcode reader in the software.

These steps will be explained in the upcoming chapters.

6 (29) Installation Manual Version 004

Introduction

Installation Manual Version 004 7 (29)

Adjusting the Z-

Tower Height 2

General 2.1

Before the barcode reader can be used with the Gilson Liquid Handler´s robot

arm, the working height of the Z-tower must be changed.

Hardware 2.2

The barcode reader set contains two black anodized (i.e. has a black eloxation

layer) adjustment cylinders (one with a height of 127 mm, one with a height of 175

mm), which aid you in mounting the Z-tower at the correct height. The 175 mm ad-

justment cylinder is used for the adjustment when cooling racks are used. In either

case the appropriate value must be set up in the Gilson Control Software.

Be sure that you have the correct adjustment cylinder (black anodized). The

original gray cylinder has a height of only 124 mm and this may cause prob-

lems when reading barcodes!

The first step in modifying the working height of the Z-tower is to turn the power off

and remove all the racks from the Gilson Liquid Handler´s bed. Move the robot

arms to the center of the bed as shown in "The Gilson Liquid Handler" on page

To achieve the 127 mm (or 175 mm when using a cooling rack) height, loosen the

screw on the back of the Z-tower and pull the Z-tower upwards slightly. Place the

black anodized adjustment cylinder between the bottom of the Z-tower and the

floor of the Liquid Handler´s bed. Lower the Z-tower until it sits on top of the ad-

justment cylinder. The scale should then show the value 127 mm (or 175 mm

when using a cooling rack). Tighten the screw and remove the black anodized

adjustment cylinder.

8 (29) Installation Manual Version 004

Adjusting the Z-Tower Height

Figure 2.1. The Gilson Liquid Handler

Software 2.3

The new height must also be entered in the Gilson Liquid Handler software. Refer

to the corresponding Gilson Liquid Handler manual to set up this value.

Z-Tower

XY-Robot Arm

Rack

Bed

Installation Manual Version 004 9 (29)

Mounting the

Barcode Reader 3

Mounting the Barcode Reader on the Gilson Liquid Handler 3.1

1. Mount the barcode reader on the barcode reader bracket using the 2 M3x8

hexagon screws, as shown below:

Figure 3.1. Mounting the Barcode Reader to the Bracket

2. Mount the barcode reader bracket on the Z-tower of the Gilson Liquid Handler

using the two M3x10 cylinder screws. Ensure that you mount the bracket just

under the top plate of the Z-tower cover. There should not be a gap between

the Z-tower cover and the bracket (as shown in Figure 3.2.).

2x M3x8

Hexagon

Screws

Barcode Reader Bracket

10 (29) Installation Manual Version 004

Mounting the Barcode Reader

Figure 3.2. Mounting the Barcode Reader Bracket

3. Affix the two adhesive fasteners to the Z-tower arm as shown in Figure 3.3.

and fasten the barcode reader cable to them using the two plastic strips.

The lower cable socket should be placed about 20 mm above the motor and

the upper socket about 20 mm below the upper end of the Z-tower.

Cylinder

Screws

No gap!

Side View

Mounting the Barcode Reader on the Gilson Liquid Handler

Installation Manual Version 004 11 (29)

Figure 3.3. Securing the Barcode Cable

4. Affix the barcode reader cable to the Gilson Control Unit cable using the spiral

hose (see figure 3.4).

Cable fasteners

with fastening

strips

20 mm

12 (29) Installation Manual Version 004

Mounting the Barcode Reader

Figure 3.4. Securing the Barcode Reader Cable to the Control Unit Cable

Connecting the Power Supply 3.2

Connect the power supply unit to the barcode reader plug (5 mm stereo connec-

tor) and the power supply unit to a free mains outlet.

Connecting the Serial Cable 3.3

To send the reader information to the control unit, connect one end of the 10

meter serial cable to the barcode reader plug and the other end to a free serial

port (e.g. TTY09) on the CCU of the spectrometer.

Spiral Hose

Barcode Reader Cable

Gilson Control Unit Cable

Installation Manual Version 004 13 (29)

Software 4

General 4.1

The barcode reader must be setup in the TopSpin software before use. This pro-

cedure is described in this chapter.

Defining the Barcode Reader 4.2

Using the BEST-NMR Administration Toolbox select main menu item Hardware.

(see below).

To start the BEST-NMR Administration Toolbox, select one of the following possi-

bilities:

•Type bestadm in the TopSpin command line and press enter.

or:

•Type in iconnmr in the TopSpin command line.

•Select ‘Configuration’ in the window that appears.

•Click on ’Master Switches’ in the window that appears (left side).

•Click on ‘Enable BEST-NMR’, followed by ‘BEST Administration Tool [be-

stadm]’ .

Figure 4.1. BEST-NMR Administration Toolbox

The window shown in Figure 4.2. will open.

14 (29) Installation Manual Version 004

Software

Figure 4.2. BEST-NMR Hardware Installation

Select Associated Hardware from the left panel and set the barcode reader pa-

rameters in the right panel as follows:

„Installed?“ yes

„Connected to“ means the serial port on the CCU of the spectrometer,

where you are connected to the barcode reader, e.g. tty09. Select the ap-

propriate serial port.

Press Apply and then OK. The barcode reader should now be recognized by the

software.

Programming the Barcode Reader

Installation Manual Version 004 15 (29)

Programming the Barcode Reader 4.3

The barcode reader must be programmed once after the first installation to ensure

that it reads all the barcodes correctly. This step will calibrate the scanner’s posi-

tion. The system will search different positions to find the optimal position for bar-

code reading. This position is then saved in the file SCANNER.SRC. The

calibration takes about 5-10 minutes.

Click on the menu item Tests in the BEST-NMR Administration Toolbox (see fig-

ure 4.1). The following window (figure 4.3) will then open:

Figure 4.3. BEST-NMR Test Tool

Select the button Configure on the bottom left panel to program the barcode

reader. The following window (figure 4.4) will appear:

Figure 4.4. Configuration Instructions

Click ’Load’ only when initializing the barcode reader for the first time. If reconfig-

uring after a TopSpin software update, click on ’Donot’.

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Software

Installation Manual Version 004 17 (29)

Barcodes 5

General 5.1

This chapter provides information about the barcodes and the barcode labels

used in the Gilson BEST system.

Label Size 5.2

The BEST system is designed to work with barcode labels with a size of 70 mm x

35 mm, e.g. No 3422 from Zweckform.

General Barcode Information 5.3

Each label which is adhered on a rack or wellplate must have two barcodes:

The upper barcode contains the Matrix ID and the lower barcode contains the Ma-

trix Type.

Notice:

1. The term „Matrix“ is the generic term used for racks and wellplates. So Rack

Type and Wellplate Type are both Matrix types, and likewise Rack ID and Well-

plate ID are both matrix ID´s.

2. If a TECAN preparation robot is also used to read the Wellplate ID, the bar-

code for this robot must also be placed in the free space located in the lower

part of the wellplate labels as shown in Figure 5.1.).

It is recommended that you also place the barcode information as human-

readable text on the labels.

Barcode type Code 128 is used for all barcodes. Other barcode types are ignored

by the barcode reader due to its initialization sequence.

18 (29) Installation Manual Version 004

Barcodes

Figure 5.1. Matrix ID and Matrix Type on a Wellplate Label Example

Matrix ID 5.4

The Matrix ID is a user specific, unique ID which may contain a three-character

company name, followed by a serial number, and optionally by the analytical

method divided by a hash - character ( # ).

The Matrix ID is the first part of the spectrometer search string for order

files for this matrix.

Example: When the label with the Matrix ID „XYZ000001#NMR“ (which repre-

sents company XYZ´s first NMR experiment) is placed on a wellplate, all order

files which start with the string „XYZ000001#NMR“ (e.g. XYZ000001#NMR A1,

XYZ000001#NMR A2 etc.) are associated with this wellplate.

Matrix Type 5.5

The Matrix Type is a string in a specific format which contains information about

the type and geometry of the rack, the position of samples in the rack, and the

numbering of the sample in the rack (arrangement).

The first 5 characters of the string are a code representing the rack type used, fol-

lowed by a hash character ( # ), e.g. 209 # for a rack code 209 or WH12# for a

wellplate having positions from A to H and from 1 to 12.

Matrix Type

MatrixID

Optional

Matrix ID

on the wellplate

label useful only

for TECAN

reparation robot;contains the sameinformationas thebarcodeonthe top ofthelabel

Matrix Type

Installation Manual Version 004 19 (29)

Following the Matrix Type code is a character which represents the arrangement.

This character is encoded as follows:

Table 5.1. Encoding the Arrangement

Upper Left

(ul) Upper Right

(ur) Lower Left

(ll) Lower Right

(lr)

Horizontally

Stacked

(hs)

ulhs =Code 0 urhs =Code 1 llhs =Code 2 lrhs =Code 3

Horizontally

Folded

(hf)

ulhf =Code 4 urhf =Code 5 llhf =Code 6 lrhf =Code 7

Vertically

Stacked

(vs)

ulvs =Code 8 urvs =Code 9 llvs =Code A lrvs =Code B

Vertically

Folded

(vf)

ulvf =Code C urvf =Code D llvf =Code E lrvf =Code F

Table 5.2. List of Relevant Matrix Types

211 #wash 304B#wash 306B#wash WH12#A

209 #8 209B#8 216 #8 770 #8

205 #wplt 668 #wplt 526 #8 205MI#8

WH12@1MM#A 348B#8 WD6 #8

20 (29) Installation Manual Version 004

Barcodes

Barcode Positioning 5.6

It is necessary to always place the barcodes in the correct position on the label to

ensure correct barcode reading:

Figure 5.2. Barcode positions on the barcode label

The following specifications should be met by the barcodes:

Table 5.3. Maximum Barcode Dimensions

Variable Minimum Optimal Maximum

a 0 mm 1mm 2 mm

b 7 mm 8 mm 10 mm

c 0.5 mm

d 8.5 mm 9.5 mm 10.5 mm

e 16.5 mm 17.5 mm

f9mm10mm

g 0 mm 2 mm 3 mm

h 5 mm 7 mm 15 mm

i45mm

j5mm

35mm

hij

70 mm

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