Cole Parmer Cryo-Blade CG-900 User manual

coleparmer.com
CG-900 Cryo-Blade™
Cryogenic Grinder
Operating Manual

Operating Manual
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1300-87082-1M
Contents
1.0 Introduction.................................................................................................................................................... 4
1.1 The Cryo-Blade ........................................................................................................................................................4
1.2 Accessories...............................................................................................................................................................5
1.3 Main Features...........................................................................................................................................................6
2.0 Precautions .....................................................................................................................................................7
3.0 Installation...................................................................................................................................................... 8
3.1 Location......................................................................................................................................................................8
3.2 Unpacking ...............................................................................................................................................................10
3.3 Connection – Electrical........................................................................................................................................10
3.4 Connection – Exhaust ..........................................................................................................................................10
3.5 Connection – Liquid Nitrogen............................................................................................................................. 11
3.6 Leveling.................................................................................................................................................................... 12
3.7 Setting Up................................................................................................................................................................ 12
3.8 Questions.................................................................................................................................................................12
4.0 Cryogenic Comminution.............................................................................................................................12
4.1 Safety and Handling .............................................................................................................................................12
4.2 Cryogenic Basis ..................................................................................................................................................... 13
5.0 How the Unit Works.....................................................................................................................................13
5.1 Bowl Manipulation................................................................................................................................................. 13
5.2 Grinding Methods.................................................................................................................................................. 15
5.3 Liquid Nitrogen Dispensing ................................................................................................................................ 17
5.4 Dry Ice Operation .................................................................................................................................................. 17
5.5 Grinding.................................................................................................................................................................... 17
6.0 Using the Cryo-Blade.................................................................................................................................. 17
6.1 Loading Bowl in the Unit...................................................................................................................................... 17
6.2 Safety Features ...................................................................................................................................................... 18
6.3 Taring the Load Cell.............................................................................................................................................. 18
6.4 The Main Screen.................................................................................................................................................... 18
6.5 Loading a Protocol ...............................................................................................................................................20
6.6 During a Cycle........................................................................................................................................................ 21
6.7 General Comminution Using Liquid Nitrogen...............................................................................................22
6.8 General Comminution Using Dry Ice............................................................................................................... 23
7.0 Programming the Cryo-Blade (Advanced) ............................................................................................. 24
7.1 Unlocking the Advanced Mode........................................................................................................................ 24
7.2 Managing Grinding Methods.............................................................................................................................25
7.3 Preparing a Grinding Program ..........................................................................................................................26
8.0 Global Parameters ......................................................................................................................................26
8.1 Options.................................................................................................................................................................... 27

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9.0 Special Parameters.....................................................................................................................................28
9.1 Accessing the Special Parameters ..................................................................................................................28
9.2 Parameters Descriptions ....................................................................................................................................28
10.0 Troubleshooting ..........................................................................................................................................29
11.0 Periodic Inspection .....................................................................................................................................29
11.1 Warning ...................................................................................................................................................................30
11.2 Inspection Schedule Table.................................................................................................................................. 31
12.0 Service Operations ......................................................................................................................................31
12.1 Warning .................................................................................................................................................................... 31
12.2 Back Panel Removal ............................................................................................................................................. 31
12.3 Left Panel Removal............................................................................................................................................... 32
12.4 Right Panel Removal............................................................................................................................................ 33
13.0 Firmware and Program Transfers ............................................................................................................ 34
13.1 Preset Protocols.................................................................................................................................................... 34
13.2 Backup or Restore by USB................................................................................................................................. 34
14.0 Technical Specifications ............................................................................................................................35
15.0 Warranty ....................................................................................................................................................... 36
15.1 Product Changes.................................................................................................................................................. 36
15.2 Return Shipments ................................................................................................................................................. 36
16.0 Contacting Cole-Parmer ............................................................................................................................36
© Cole-Parmer Instrument Company. All rights reserved.

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1.0 Introduction
Thank you for choosing the CG-900 Cryo-Blade Cryogenic Grinder from Cole-Parmer. To ensure years of reliable,
ecient, and safe use of this instrument, please read this manual thoroughly and keep it in a safe and handy place
for future reference.
Should you have any question regarding the use, maintenance or repair of your instrument, kindly contact
Cole-Parmer directly for assistance (see page 36 for contact details).
This section intends to introduce the reader to basic grinding principles and to familiarize them with the Cryo-Blade.
1.1 The Cryo-Blade
General View
LCD screen interface
5 L Bowl
Emergency stop button
Load cell
Handle
Working Principle
The Cryo-Blade is an automatic cryogenic knife mill. The Cryo-Blade uses a spinning blade to comminute
samples that have been cryogenically frozen to produce a fine homogeneous powder. By working at cryogenic
temperatures, the instrument embrittles the sample to improve grinding, even for materials that are dicult to
reduce to powder otherwise.
The Cryo-Blade automatically dispenses the correct amount of liquid nitrogen each time. Avoiding the risks
associated with handling liquid nitrogen for the user. Since the Cryo-Blade controls and monitors the input of liquid
nitrogen, all grindings are highly repeatable, increasing the value of all data collected using its sample preparation
method. Alternatively, it is also possible to use dry ice to prepare the sample.
The high-volume bowl of the Cryo-Blade can hold up to 5 liters of material. The high volume allows the user to
process large sample amounts in a single run without the need to do multiple small batches.

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The bowl of the Cryo-Blade is composed of an outer insulated shell and an inner stainless steel bowl. The inner
bowl can be removed for ease of cleaning and handling. A new inner bowl (order separately) can then be loaded in
the outer shell to be ready to use. The insulated shell helps to keep the grinding chamber at cryogenic temperatures
well after the grinding and helps reduce cryogen consumption.
The Cryo-Blade uses a load cell to measure the quantity of sample to be processed. The sensor is also used to
adjust the quantity of liquid nitrogen during the freezing process. With its smart dispensing feature, the Cryo-Blade
can help you consume less liquid nitrogen and optimize your workflow.
The Cryo-Blade bowl is equipped with a thermocouple placed directly between the bowl and the insulation to
monitor the temperature of the sample. By monitoring the temperature of the bowl, the Cryo-Blade can make sure
the sample remains at a temperature optimal for cryogenic grinding.
The Cryo-Blade operation is simple with the LCD touch screen to control the grinding process. Grinding can be
adjusted to the user preference using the preset protocols as template or can be automatically controlled by the
unit and adjusted based on the amount of sample loaded into the bowl. Using liquid nitrogen or dry ice produces
a high volume of gas (N, CO) as it evaporates. The Cryo-Blade is therefore equipped with an exhaust system to
evacuate the gases produced. The exhaust can be directly connected to the building’s main exhaust system or
placed under a vent hood.
Bowl Assembly
Bowl lid
Blade
Seal
Inner bowl
Insulated outer bowl
Bowl handle
Locking lever
1.2 Accessories
The Cryo-Blade requires the use of a specially designed bowl. This bowl is composed of the inner stainless steel
bowl and an outer insulated bowl and lid. Additional inner bowls can be purchased separately to improve workflow
and process multiple samples in a sequence and store them for subsequent analysis.
Cole-Parmer can also provide all the required equipment to handle liquid nitrogen like cryogenic Dewar, cryogenic
hose, fitting, and cart. Please contact us for help with this setup.