cytiva Amersham TYPHOON User manual
Table of Contents
1 Introduction ........................................................................................................ 4
1.1 Important user information ....................................................................................................................... 5
1.2 About this manual ......................................................................................................................................... 6
1.3 Abbreviations .................................................................................................................................................. 7
2 Safety instructions ............................................................................................. 8
2.1 Radiation ........................................................................................................................................................... 9
2.2 Safety precautions ........................................................................................................................................ 11
2.3 Labels and symbols ....................................................................................................................................... 19
2.4 Emergency procedures ............................................................................................................................... 23
3 System description ............................................................................................ 25
3.1 System overview ............................................................................................................................................ 26
3.2 Illustrations ..................................................................................................................................................... 29
3.3 Stages ................................................................................................................................................................. 33
3.3.1 Overview of stages ....................................................................................................................................... 34
3.3.2 Fluor stage with accessories ................................................................................................................... 35
3.3.3 Phosphor stage with accessories .......................................................................................................... 39
3.3.4 Multi stage with accessories ................................................................................................................... 41
3.4 Filters .................................................................................................................................................................. 43
4 Amersham TYPHOON Control Software .......................................................... 44
4.1 Main window .................................................................................................................................................... 45
4.2 Scanning modes ............................................................................................................................................. 51
4.3 General settings ............................................................................................................................................. 54
4.4 Scan area functions ....................................................................................................................................... 57
4.5 Image file settings ......................................................................................................................................... 61
4.6 View Image mode .......................................................................................................................................... 62
5 Installation .......................................................................................................... 65
5.1 Site requirements .......................................................................................................................................... 66
5.2 Delivery and transport ................................................................................................................................. 69
5.3 Setup ................................................................................................................................................................... 70
5.4 Connections ..................................................................................................................................................... 72
6 Operation ............................................................................................................ 74
6.1 Preparing the Amersham TYPHOON scanner ................................................................................... 76
6.1.1 Starting the system ..................................................................................................................................... 77
6.1.2 Changing the filters ..................................................................................................................................... 79
6.1.3 Custom filters ................................................................................................................................................. 83
6.2 Preparing the sample ................................................................................................................................... 84
6.2.1 Preparing a sample for fluorescence imaging ................................................................................. 85
6.2.2 Preparing a sample for phosphor imaging ........................................................................................ 89
6.2.3 Preparing a sample for densitometry .................................................................................................. 95
6.2.4 Recommended scan settings for densitometry, NIR scans, and multi-well titer plates 96
6.3 Performing a scan .......................................................................................................................................... 98
6.4 Procedures after a scan ............................................................................................................................... 102
Table of Contents
Amersham TYPHOON Operating Instructions 29193226 AF 2
7 Maintenance ....................................................................................................... 104
8 Troubleshooting ................................................................................................. 106
8.1 General errors and warnings ..................................................................................................................... 107
8.2 Error messages ............................................................................................................................................... 108
8.3 Troubleshooting guide ................................................................................................................................. 110
9 Reference information ....................................................................................... 111
9.1 Specifications .................................................................................................................................................. 112
9.2 Software ............................................................................................................................................................ 115
9.3 Recycling information .................................................................................................................................. 116
9.4 Regulatory information ............................................................................................................................... 117
9.4.1 Contact information .................................................................................................................................... 118
9.4.2 European Union and European Economic Area .............................................................................. 119
9.4.3 Great Britain ................................................................................................................................................... 120
9.4.4 Eurasian Economic Union (Евразийский экономический союз) .......................................... 121
9.4.5 North America ............................................................................................................................................... 123
9.4.6 Regulatory statements .............................................................................................................................. 124
9.4.7 China .................................................................................................................................................................. 125
9.4.8 Other regulations and standards .......................................................................................................... 128
9.5 Ordering information ................................................................................................................................... 129
9.6 Health and Safety Declaration Form ...................................................................................................... 130
Index ........................................................................................................................... 132
Table of Contents
Amersham TYPHOON Operating Instructions 29193226 AF 3
1 Introduction
About this chapter
This chapter contains important user information, abbreviations used within the
manual, and intended use of the Amersham™ TYPHOON system.
In this chapter
Section See page
1.1 Important user information 5
1.2 About this manual 6
1.3 Abbreviations 7
1 Introduction
Amersham TYPHOON Operating Instructions 29193226 AF 4
1.1 Important user information
Read this before operating the
product
All users must read the entire Operating Instructions before installing, oper-
ating, or maintaining the product.
Always keep the Operating Instructions at hand when operating the product.
Do not install, operate, or perform maintenance on the product in any other way than
described in the user documentation. If you do, you may be exposed or expose others
to hazards that can lead to personal injury and you may cause damage to the equip-
ment.
Intended use of the
Amersham TYPHOON scanner
The Amersham TYPHOON scanner is a versatile laser scanner that can be used for
many different imaging applications, including:
•imaging of storage phosphor screens
•fluorescence imaging with multiple lasers, for example for muliplex Western blot-
ting and two-dimensional difference gel electrophoresis (2D DIGE)
•imaging of gels with colorimetric stains
•densitometric imaging, for example of Coomassie blue stained gels
Amersham TYPHOON scanner is intended for research use only, and shall not be used
in any clinical procedures or for diagnostic purposes.
Prerequisites
In order to operate Amersham TYPHOON scanner in the way it is intended, the
following prerequisites must be fulfilled:
•You must have read and understood the safety instructions in the user documenta-
tion.
•All operations should be performed by qualified personnel who are adequately
trained.
•Amersham TYPHOON scanner must be installed in accordance with the site
requirements and instructions in Chapter 5 Installation, on page 65.
1 Introduction
1.1 Important user information
Amersham TYPHOON Operating Instructions 29193226 AF 5
1.2 About this manual
Purpose of this manual
This manual provides information needed to install, operate and maintain the product
in a safe way.
Scope of this manual
The Operating Instructions cover all configurations of Amersham TYPHOON scanner
and the Amersham TYPHOON Control Software. The illustration below shows the
Amersham TYPHOON scanner.
Descriptions of Amersham ERASER and Amersham Cabinet are not covered in this
document.
Notes and tips
Note: A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip: A tip contains useful information that can improve or optimize your proce-
dures.
Typographical conventions
Software items are identified in the text by bold italic text.
Hardware items are identified in the text by bold text.
Tip: The text can include clickable hyperlinks to reference information.
1 Introduction
1.2 About this manual
Amersham TYPHOON Operating Instructions 29193226 AF 6
1.3 Abbreviations
The abbreviations used in this Operating Instructions are defined as follows:
Abbrevia-
tion
Description Translation
2D DIGE Two-dimensional Difference
Gel Electrophoresis
Two-dimensional Difference
Gel Electrophoresis
OD Optical density Optical density
PMT Photo-multiplier tube Photo-multiplier tube
RGB Red Green Blue Red Green Blue
RH Relative humidity Relative humidity
RI Radioisotope Radioisotope
SHG Second harmonic generation
nonlinear optical process
Second harmonic generation
nonlinear optical process
TP Titer plate Titer plate
UV Ultraviolet radiation Ultraviolet radiation
NIR Near-infrared radiation Near-infrared radiation
1 Introduction
1.3 Abbreviations
Amersham TYPHOON Operating Instructions 29193226 AF 7
2 Safety instructions
About this chapter
This chapter describes safety precautions, labels and symbols that are attached to the
equipment. In addition, the chapter describes emergency and recovery procedures,
and provides recycling information.
In this chapter
Section See page
2.1 Radiation 9
2.2 Safety precautions 11
2.3 Labels and symbols 19
2.4 Emergency procedures 23
Important
WARNING
Before installing, operating or maintaining the product, all
users must read and understand the entire contents of this
chapter to become aware of the hazards involved.
2 Safety instructions
Amersham TYPHOON Operating Instructions 29193226 AF 8
2.1 Radiation
Radiation hazard prevention
This instrument is not equipped with any radioisotope or radiation generating unit, and
is therefore not regulated by radiation hazard prevention laws. However, the instru-
ment is capable of scanning storage phosphor screens which may be polluted by radio-
isotopes.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument
immediately and follow the instructions of your radiation adminis-
trator.
Controlled area
Paragraph 1 of Article 1 of the Law Enforcement Rules for Prevention of Radiation
Hazards due to Radioisotope and so forth (Prime Minister's Office ordinance No. 56)
defines the controlled area as "a place where the dose equivalent related to external
radiation exceeds the dose equivalent determined by the Director General of the
Science and Technology Agency (hereinafter referred to as the Director General), the
concentration of radioisotope in the air exceeds the concentration determined by the
Director General, or the radioisotope density on the surface polluted by radioisotope
exceeds the density determined by the Director General."
Limit of superficial pollution
Paragraph 3 of Article 4 of Notice No. 15 of the Science and Technology Agency that
determines the quantity, etc. of radiating isotope specifies that the density of radioiso-
tope on the surface polluted by radioisotope must be one tenth of the density defined
in Article 8.
Article 8 and Table 3 of this Notice define the limits as shown below:
1. Superficial density of radioisotope that radiates alpha rays: 4 Bq/cm2
2. Superficial density of radioisotope that does not radiate alpha rays: 40 Bq/cm2
Installation site of instrument
This instrument is capable of scanning not only storage phosphor screens but also
fluorescent pigment label samples (non-RI method). Therefore, it is recommended that
the user should install it outside the controlled area and use RI-indicated samples
without contacting them with storage phosphor screens directly.
However, as described above, the storage phosphor screen surface can be polluted by
radioisotope (RI), depending on the sample condition, since the instrument sticks the
sample to the 3H-compatible storage phosphor screen surface and exposes it in an
auto-radiography experiment of the 3H label sample.
2 Safety instructions
2.1 Radiation
Amersham TYPHOON Operating Instructions 29193226 AF 9
The degree of superficial pollution of the storage phosphor screen is greatly influenced
by the dryness of the sample and dose of radioisotope in an experiment and can
exceed the limits mentioned in the section above.
When the instrument reads a storage phosphor screen with a polluted non-exposure
area, it can be polluted. The degree of such superficial pollution greatly differs with
users' operation conditions. Superficial pollution can exceed the limit mentioned
above.
Note: As mentioned above, install this instrument in the RI controlled area if the
user uses RI-indicated samples that will be in direct contact with storage
phosphor screens.
Removal from the controlled area
If it is necessary to move the instrument and its laboratory, which were installed and
have been used in the controlled area, from the controlled area, make sure that the
degree of the superficial pollution is below the limits mentioned in Limit of superficial
pollution, on page 9 above.
2 Safety instructions
2.1 Radiation
Amersham TYPHOON Operating Instructions 29193226 AF 10
2.2 Safety precautions
Introduction
Amersham TYPHOON scanner is powered by mains voltage and handles materials that
can be hazardous. Before installing, operating or maintaining the system, you must be
aware of the hazards described in this manual.
Follow the instructions provided to avoid personal injuries, damage to the
product, or to other personnel and equipment in the area.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Personal protection
•Power supply
•Installing and moving the system
•System operation
•Maintenance
Definitions
This user documentation contains safety notices (WARNING, CAUTION, and NOTICE)
concerning the safe use of the product. See definitions below.
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 11
General precautions
WARNING
Do not use the equipment if smoke, strange noises or strange
odors can be perceived, or if the equipment becomes unusually
hot. This can result in fire or electric shock.
Stop using the equipment immediately, turn off the power switch
and unplug the equipment from the power outlet. Contact your
local Cytiva representative to request repair.
WARNING
Do not damage the power supply cord by bending, twisting, heating
or allowing them to become pinned under the equipment. Using
damaged power cords could result in fire or electric shock.
If the power supply cords are damaged, contact your local Cytiva
representative for replacements.
WARNING
Do not allow liquids, flammable materials or metallic objects to get
into the product. This may result in fire or electric shock.
In case of malfunction, turn off the power switch, unplug the equip-
ment from the power outlet, then contact you local Cytiva repre-
sentative.
WARNING
Never detach the inner cover screwed to this instrument. If it is
detached, laser beam may leak with a risk of loss of vision.
WARNING
Never modify the interlocks in this instrument, laser beam may leak
with a risk of loss of vision.
CAUTION
If radioisotope (RI) pollution occurs, stop use of the instrument
immediately and follow the instructions of your radiation adminis-
trator.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 12
CAUTION
Use of controls or adjustments or performance of procedures other
than those specified herein may result in hazardous radiation
exposure.
NOTICE
Avoid any shock or vibration to the equipment, as this may damage
the equipment.
Personal protection
CAUTION
Always wear gloves, protective glasses and a lab coat or similar
when handling samples.
CAUTION
Always wear cotton gloves when handling storage phosphor
screens.
Power supply
WARNING
Do not use the equipment with a power supply other than that
recommended. Fire and electric shock could result.
WARNING
The product must always be connected to a grounded power
outlet.
WARNING
Do not block access to the power switch and power cord. The
power switch must always be easy to access. The power cord with
plug must always be easy to disconnect.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 13
WARNING
Connect the power supply directly to a grounded wall power outlet.
The use of extension cords or multiple loads on one electrical outlet
could result in fire and electric shock.
WARNING
Only use power cords delivered or approved by Cytiva.
CAUTION
Do not use the same power supply as that of large equipment such
as an air conditioner or centrifuge. Malfunction could result.
Installing and moving
WARNING
Do not block the ventilation inlets or outlets on the system.
WARNING
Do not place the equipment on unstable tables or on inclined
surfaces, as the equipment could be dropped or fall, resulting in
injury.
CAUTION
Only authorized service personnel are allowed to install or move
the product. Contact your local Cytiva representative for help and
advice.
CAUTION
Install the product in a location where it will not come into contact
with water or chemicals.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 14
CAUTION
•Do not connect any USB devices other than the product to the
computer in which the Amersham TYPHOON Control Software
is installed. Malfunction could result.
•Use only the Amersham TYPHOON Control Software during
scanning.
CAUTION
Connect the computer hardware on the same power circuit as the
scanner, otherwise the equipment may be influenced by electrical
noise.
CAUTION
Do not block the cooling fans. If they are blocked, the instrument
may malfunction.
NOTICE
Any computer used with the equipment must comply with
IEC 60950 or IEC 62368-1 and be installed and used according to
the manufacturer's instructions.
Operation
WARNING
Do not use the instrument within or near a sink, or in humid (above
70% RH) or dusty environments. Fire and electric shock could
result.
WARNING
When opening or closing the main scanner door, make sure that no
objects or body parts are caught in the main scanner door.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 15
CAUTION
Do not place heavy objects on the instrument, these may fall off
and cause injury.
CAUTION
Do not open the main scanner door or filter door while the device is
in operation. Injury could result.
CAUTION
Do not scratch or drop parts containing glass such as lenses, filters
or lights.
CAUTION
Handle the sample stages and the digitization plate with care to
avoid dropping them.
CAUTION
Be careful not to break the glass surface of the fluor stage.
CAUTION
Reagents used to prepare the sample should be used in accord-
ance with the manufacturer instructions.
CAUTION
Do not leave samples in the instrument after scanning. If left, these
may degrade and cause damage to the instrument.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 16
CAUTION
Do not insert a storage phosphor screen in the product before
turning on the machine. If a storage phosphor screen is detected
during the self-diagnosis of the product, the sensitivity of the
storage phosphor screen can deteriorate and reduce the quality of
the scanned data.
CAUTION
Do not turn off power during operation as this can cause loss of
data in the internal memory. Only turn off power in an emergency
situation.
CAUTION
Do not use the instrument within or near a sink, or in humid (above
70% RH) or dusty environments. This can result in data failure.
NOTICE
The automatic sleep mode of the computer should be turned off to
prevent the computer from entering sleep mode during a scan.
Otherwise loss of data might occur.
Maintenance
WARNING
Do not attempt to modify the instrument, or fire and electric shock
could result.
WARNING
Do not use excessive amounts of liquids for cleaning the product,
this may result in product malfunction or electric shock.
CAUTION
Wear gloves to prevent direct contact with chemical substances.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 17
CAUTION
Take care when connecting the power supply cable. Do not tug on
the cable, and do not handle the connection plugs with wet hands.
CAUTION
Turn off the power switch and remove connecting cables before
moving the equipment.
CAUTION
Turn the power switch off before cleaning the inside of the equip-
ment.
CAUTION
Unplug the equipment if it will not be used for an extended period.
2 Safety instructions
2.2 Safety precautions
Amersham TYPHOON Operating Instructions 29193226 AF 18
2.3 Labels and symbols
Introduction
This section describes the nameplate, labels, and other safety and regulatory informa-
tion attached to the product.
Nameplate
The nameplate provides information about the model, manufacturer, and technical
data.
An example of Amersham TYPHOON label is reported below.
Label text Description
Model Instrument model
Serial number Instrument serial number
Manufacturing Year and
Month
Manufacturing year and month
Frequency Supply voltage frequency
Supply Voltage Supply voltage
Max Current Max. current consumption
Protection class Protection class. Ingress protection according to IEC
60529.
Alternating current
Warning! Read the user documentation before using
the system. Do not open any covers or replace parts
unless specifically stated in the user documentation.
2 Safety instructions
2.3 Labels and symbols
Amersham TYPHOON Operating Instructions 29193226 AF 19
Labels concerning lasers
Label Meaning
Warning! The instrument contains lasers
and service is only allowed by trained
service engineers. Do not modify the
instrument in any way.
Complies with FDA performance
standards for laser products except for
conformance with IEC 60825-1 Ed. 3., as
described in Laser Notice No. 56, dated
May 8, 2019.
Manufactured by XXX X
405N200231C
This product complies with FDA perform-
ance standards for laser products except
for conformance with IEC 60825-1 Ed. 3.,
as described in Laser Notice No. 56, dated
May 8, 2019.
The following labels describe that the instrument is a class 1 Laser Product
(IEC60825-1/EN60825-1). This instrument meets the laser radiation safety require-
ments specified in the Code of the Federal Regulations (21 CFR, Chapter 1, Subchapter
J).
CLASS 1
LASER PRODUCT
IEC60825-1:2014
CLASS 1
LASER PRODUCT
EN60825-1:2014+A11:2021
The labels in French are reported below.
APPAREIL À
LASER DE CLASSE 1
EN60825-1:2014+A11:2021
Other safety labels
Label Meaning
This device complies with part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and
(2) this device must accept any interference received,
including interference that may cause undersired operation.
CAN ICES-3 (A)/NMB-3(A)
This device complies with part 15 of the FCC rules.
CAN ICES-3 (A)/NMB-3 (A)
2 Safety instructions
2.3 Labels and symbols
Amersham TYPHOON Operating Instructions 29193226 AF 20
Table of contents
