Drummond Nanoject II User manual

Nanoject II
Digital Control Box
MADE IN USA
INSTRUCTION MANUAL • 3-000-029-D
LABORATORY EQUIPMENT
E21997

2
• For indoor use only.
• Never operate unit in an explosive atmosphere.
• Do not operate unit with a damaged cord.
• Use power source only in a standard electrical outlet.
• Do not handle power source with wet hands.
• Do not put unit or power source in water or other liquid.
• When servicing, use only identical Drummond replacement parts.
• Save these instructions.
If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired. Always use
the power source that is supplied with the unit.
Specifications for Usage
This unit is intended to be used to inject nanoliter quantities of sample.
This equipment is for indoor use only.
Temperature Range 10°C–35°C, Maximum Humidity 60%
FAILURE TO USE THE EQUIPMENT IN ACCORDANCE WITH INSTRUCTIONS
OR MODIFYING THE EQUIPMENT WILL VOID WARRANTIES.
CAUTION: CAREFULLY READ THROUGH THIS ENTIRE MANUAL BEFORE USING
YOUR NANOJECT II. PAY CLOSE ATTENTION TO THE RULES FOR SAFE
OPERATION WARNINGS AND CAUTIONS.
Rules for Safe Operation
Connections to Power Source:
Check the power source to see that the line voltage corresponds to the voltage
indicated on the mains adapter.
If the mains adapter source and the line voltage are not compatible, the electrical
components of the Nanoject II may be damaged or destroyed.
Before each use, confirm that the mains adapter cable is not damaged, worn or
severely buckled and there are no breaks in the insulating surface. If any damage is
noted, do not use the Nanoject II until the damaged main is replaced.
Connecting to power outlet: The control box has a power source transformer
attached to the box. Plug this into your outlet using the correct adapter supplied.
There is an “on/off” switch on the side of the control box which turns on the control
box once the injector head is attached. To turn off, use the switch to turn off. If you
want to disconnect the power from the unit, merely unplug the transformer from the
wall outlet.
Power/Current Rating: AC 100~V, 50-60 Hz, 38VA DC 12V 2A
Input 100-240~V, 50-60 Hz, 0.6A
Output 12V 2A

Introduction
Thank you for purchasing the Nanoject II Control Box by Drummond Scientific Company.
This device can be used immediately after purchase. A summary of how to operate the
device is provided in this quick instruction manual.
Accessories available for purchase:
Replacement Glass 3.5˝ (vial of 100) . . . . . . . . . . . . . . . . . . . . . . . .3-000-203-G/X
Replacement Glass 7˝ (vial of 100) . . . . . . . . . . . . . . . . . . . . . . . . .3-000-203-G/XL
O-ring Kit with Collet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-002-KN
Wire Plunger (replacement) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-000-203-X
30G/2˝ Needle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-027
Footswitch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-032
(remote capability for fill, empty and inject functions)
Micromanipulator, MM33 (right hand) . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-R
(left hand) . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-L
Support Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-025-SB
Magnetic Base . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-025-MB
Universal Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-000-024-A
Part Number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-000-029-D
Power Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100/240 volt, 50/60 Hz
Total Sample Volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4.2 nL
Fill Volume Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 nL or 46 nL/sec
Empty Volume Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 nL/sec (only)
Injection Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 nL or 46 nL/sec
Volume Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.3 nL - 69.0 nL
Plunger Travel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 mm
Glass Micropipette Dimensions . . . . . . . . . . . . . . . . . . . . . . OD 0.045˝ (1.14 mm)
ID 0.021˝ (0.53 mm)
Control Box Dimensions
Weight . . . . . . . . . . . . . . . . . . . . . 0.43 kg
Length . . . . . . . . . . . . . . . . . . . . . 14 cm
Width . . . . . . . . . . . . . . . . . . . . . . 13.5 cm
Thickness . . . . . . . . . . . . . . . . . . 4 cm
Technical Specifications
3

4
Quick Start Guide
1) Plug the injector cable into the “HEAD” port on the right side of the control box.
2) If using the footswitch, insert the footswitch cable into the “FOOTSWITCH” port on
the right hand side of the control box.
3) Plug the control box into a standard electrical outlet using the appropriate
adapter head on the power supply.
4) Turn on unit with the on/off switch located on the left hand side of the control box.
Initially the Drummond logo will be displayed on the screen followed shortly by the
Operational Mode screen.
5) Micropipette Pulling and Backfilling
The Nanoject II requires the use of pulled micropipettes from the glass provided.
Ideally, the tip size should be 10-30 microns in size. The capillary glass provided is
produced from N-51-A material and has a softening point of 780°C. Many researchers
pull the tips and then break them off with forceps. This enables piercing the cell
membrane much easier with no skipping. Once the tips are pulled, they must be
“backfilled” with oil (or other non-compressible fluid) before attachment to the injector.
Silicone or mineral oil is frequently used. Backfilling is facilitated by using the 30 gauge
x 2˝ needle and a syringe. Disposable spinal needles are also frequently used.
NOTE: THE NANOJECT II WI NOT OPERATE PROPER Y WITHOUT
BACKFI ING THE MICROPIPETTE.
6) Securing the Micropipette to the Injector Standard Collet/O-ring (Fig. 1)
Once the micropipette is backfilled, loosen the collet. The pointed wire plunger should
be positioned so you can just see the tip flush with the end of the collet (slightly
recessed is also acceptable). This is referred to as the “Home” position. Push them
micropipette onto the wire plunger and as you push the tip on, feel it go through the
large O-ring and seat in the white spacer. Once positioned, tighten the collet securely.
Give the micropipette a pull to confirm it is securely mounted. See Fig. 1 for proper
configuration of the O-rings and the white spacer. It is absolutely essential that these
components are properly configured.
NOTE: THE WHITE SPACER HAS ONE F AT SIDE AND ONE SIDE WITH A
RECESS MACHINED AROUND THE HO E. THIS RECESS IS TO RECEIVE THE
BACK END OF THE PIPETTE.

5
7) Filling the Micropipette
First, select your fill speed by pressing the [SETUP] icon and choosing 23 or 46 n /sec.
(NOTE: EMP Y SPEED IS PRESET TO 92 n /SEC ON Y)
Return to Main Menu by pressing the [EXIT] icon.
Then enter manual mode by pressing the [MANUA ] icon.
Once the micropipette is secured to the collet, press the [EMPTY] icon. This will drive
the wire plunger out forcing oil to the tip of the pipette and any excess oil will be
expelled. Extend the plunger until an audible beep is heard. The plunger is now fully
extended (approx. 27 mm from the end of the collet).
Now place the tip of the pipette into your sample and press the [FI ] icon. The
plunger will retract drawing with it the sample. The micropipette when fully filled, will
contain approximately 4.2 µl of sample. At any time you can stop filling by pressing the
red [STOP] icon. Continued filling can be accomplished by pressing the [FI ] icon
again. The plunger is fully retracted to its preset “home” position when you hear an
audible beep. Viscous samples may require you to fill in small steps allowing the
sample to equilibrate in the tip before continued filling. Do not allow air bubbles to form
in the micropipette. These bubbles can cause inaccurate injection volumes.
NOTE The plunger will continue to extend or retract until you press [STOP], or fully
extended or retracted position is reached.
8) Injecting Sample
To inject the sample, return to the Main Menu by pressing the [EXIT] icon. Press the
[SETUP] icon then choose your Speed (n /sec), either 23 or 46 nL/sec. Then press
the [EXIT] icon. Select inject mode by pressing the [INJECT] icon. Set the desired
injection “Volume (nL)” from the displayed volumes. Press the [INJECT] icon to inject
your sample.
NOTE Multiple injections can be performed by simply pressing the [INJECT] icon
again and again.
Aluminum Barrel Plunger
Sealing O-ring (small hole)
O-ring with large hole
Collet Glass
Plastic sleeve (spacer) – Placed between
O-rings with indentation toward glass
Fig. 1

6
[ MANUA ]Mode
This mode will enable the user to
manually fill and empty the micropipet.
Pressing the [FI ] icon will retract the
wire plunger, while pressing the [EMPTY]
icon will cause the wire plunger to extend.
Operational Modes
[ INJECT ]Mode
This mode is used to do single manual
injections. It enables the user to program
an injection volume from the displayed
volumes. Press the [INJECT] icon to
inject the desired volume at the rate
selected.
Note: Multiple injections can be
performed by simply pressing the
[INJECT] icon again and again.
The injection rate is independent of the
fill rate as programmed in the [SETUP]
mode.
Press the [EXIT] icon to exit back to the
operational mode screen.
Note: [EXIT] icon is disabled while the
wire plunger is in motion.
Note: The speed of the fill can be
regulated in the [SETUP] mode and is
independent of the injection rate.
[EMPTY] has only one speed
(92 nL per second).
Press the [EXIT] icon to exit back to
the operational mode screen.
If the [FI ] or [EMPTY] icon has been
pressed and you desire to stop the action,
simply press the same icon position (now
labeled [STOP]) a second time.

7
[ PROGRAM ]Mode
This mode enables the user to program
multiple injection cycle recipes. To
program a new recipe, press the [NEW]
icon. A screen displaying the sample
injection volume (nL) and an injection
rate (nL/sec) will appear. As before,
adjust these individual values by using
the respective displayed volumes
and rates.
Pressing the [NEXT] icon brings up a screen displaying the number of injection
cycles. Again, use the [+] and [-] icons to select the value desired.
Pressing the [BACK] icon always returns you to the previously displayed screen.
Pressing the [NEXT] icon once again brings up the screen displaying the interval
(secs) between injection cycles. Use the [+] and [-] icons to set the desired time.
Pressing the [SAVE] icon allows you to view the entire program recipe values in the
following format
VO (n ) . . . . .volume of injection
RATE (n ) . . . .rate of injection
CYC ES . . . . .number of cycles this
volume will be injected
DE AY (SEC). . .time interval between
each injection

8
Note: The program recipes are automatically saved and labelled in sequential order.
You can step through the stored recipes by pressing the [<] and [>] icons. Should you
desire to delete a recipe, simply press the [DE ] icon, and the displayed recipe will
be deleted.
Pressing the [RUN] icon will initiate the currently displayed program recipe.
[SETUP]Mode
This mode will enable the user to program
and store the manual fill and injection
rates respectively to either 23 nL or 46 nL.
Note: When retracting the plunger to the “Home” position, press the STOP button
when the plunger tip is aligned with the end of the collet; plunger will not stop
automatically. (Damage to the motor may occur if plunger is not stopped at
proper “Home” position).
Press the [HOME] icon to manually retract the plunger to the “Home” position.
Press the [EXIT] icon to exit back to the operational mode screen.
Below the [RUN] icon is a box displaying the number of injection cycles remaining in
the entire program recipe. It will count down toward (0) as the multiple injection cycle
progresses. Below this “counter” box is a display of the percentage of the program recipe
remaining. It will increase to 100% as the multiple injection cycle progresses.
Press the [EXIT] icon to exit back to the operational mode screen.
After pressing the [RUN] icon, a [PAUSE]
icon appears and enables the user to
interrupt the program at any time, and
then resume the program where it
was interrupted by pressing the [RUN]
icon again.
To initiate a single cycle of the displayed
recipe, simply press the [TEST] icon once.

9
Footswitch Operation
The one-touch functions such as [EMPTY] and [FI ] cannot be done with the
footswitch. The same phenomenon will occur if you continue to push the pedals for
functions such as [EMPTY] and [FI ]. If you ease the pressure off the pedal, the
motor will stop.
[ MANUA ]Mode
In this mode, the micropipette is manually
filled or emptied.
Touch the [MANUA ] icon to go to the
MANUAL MODE screen in the diagram
below.
EMPTY Function
When you touch this icon and the icon
changes to a red [STOP] icon, the motor
continues to empty the micropipette.
When the plunger has extended completely
(approximately 23 mm from the end of the
collet), there will be a beeping sound and
the screen will return to the [EMPTY] icon
at the same time.
To stop it in the middle, push [STOP].

10
When using [INJECT] with the footswitch, please continue to push the pedal until you
hear the beeping sound.
The motor is still in operation until you hear the beeping sound.
If you ease the pressure off the pedal before you hear the beeping sound, the motor
will stop and the set volume will not be injected.
In this mode, the [FI ] and [EMPTY] pedals are disabled.
Footswitch Operation
[INJECT]Mode
On the Operation screen, select [INJECT]
mode.
Enter the manual [INJECT] conditions.
[VOLUME (NL)] = Displayed in ranges
within 2.3 nL to 69 nL.
[RATE (NL/SEC)] = Injection speed of
23 nL or 46 nL.
When setup is finished, the green
[INJECT] icon starts injection.
One push (push all the way)=one shot,
then it will change to a yellowish green
[INJECT] icon, and there will be a beeping
sound when it is finished.
[EXIT] Goes back to the operation screen.

11
Notes

©Copyright, Drummond Scientific Company, 2019, Rev. 0219 Printed in USA
500 Parkway, Box 700
Broomall, PA 19008
800/523-7480 • FAX [phone]
Website www.drummondsci.com
For Technical Assistance, call
1-800- 23-7480 or e-mail
[e-mail]
Questions: Please contact Drummond at 800- 23-7480.