Elements eONE User manual

eONE
Miniaturized One-Channel Amplifier
USER’S GUIDE
October 2015
elements
enabling technologies for Life Science

ii
Periodically check the following link for the most updated manual version: http://elements-ic.com/downloads/
Revision History
Version Preliminary
Initial Manual Created
23th January 2014
Version 1.0
Manual Released
30th July 2014
Version 1.1
Corrections made to figure 4
10th August 2014
Version 1.2
Changed manual format
Added Package section
Corrections made to figure 5
Added new software features and voltage protocols tab
descriptions
Added import into Origin® and Clampfit® instructions
Added troubleshooting guide
29th September 2014
Version 1.3
Edited/Corrected pages headings and Summary
10th October 2014
Version 1.4
Added the note in section 2.4.6 and suggestion in the
troubleshooting guide
Corrections made to figure 4
04th December 2014
Version 1.5
Updated key features
Updated figure 2 –figure 4 –figure 5
Added the note in section 3.1.10
Updated section 3.1.5
19th February 2015
Version 1.6
Updated figures for EDR 2.X
Added warning message for offset compensation
Updated section 3.1.7 with the new C and R auto estima-
tions
Updated section 3.1.8 for the new display window
Added keyboard shortcuts list
Updated eONEread.m to elementsRead.m Matlab func-
tion
5th October 2015

iii
Summary
SECTION 1 ........................................................................................................................................................................................................... 1
1Preliminary Information............................................................................................................................................................................. 1
1.1 General notes ....................................................................................................................................................................................2
1.2 Trademarks........................................................................................................................................................................................2
1.3 Instructions on disposal .....................................................................................................................................................................2
1.4 Description of safety symbols.............................................................................................................................................................3
1.5 Qualified Personnel ...........................................................................................................................................................................4
1.6 Basic knowledge required ..................................................................................................................................................................4
1.7 Proper use of the product ..................................................................................................................................................................4
1.8 Purpose of the user’s guide................................................................................................................................................................4
1.9 Scope of the operating instructions....................................................................................................................................................5
1.10 The manual is a part of the system.....................................................................................................................................................5
1.11 Figures...............................................................................................................................................................................................5
1.12 Safety instructions .............................................................................................................................................................................6
1.12.1 Installation according to the instructions ..................................................................................................................................6
1.13Notes about usage .............................................................................................................................................................................6
SECTION 2 ........................................................................................................................................................................................................... 7
2General description and connections.......................................................................................................................................................... 7
2.1 Product description............................................................................................................................................................................8
2.2 Key features.......................................................................................................................................................................................8
2.3 Package .............................................................................................................................................................................................9
2.4 Hardware setup ...............................................................................................................................................................................10
2.4.1 Front Connections.......................................................................................................................................................................10
2.4.2 Rear Connections ........................................................................................................................................................................11
2.4.3 Select the location.......................................................................................................................................................................12
2.4.4 Checking the package contents....................................................................................................................................................12
2.4.5 Checking the operating conditions...............................................................................................................................................12
2.4.6 Tipical Experimental Setup ..........................................................................................................................................................13
SECTION 3 ......................................................................................................................................................................................................... 15
3Software installation and description ....................................................................................................................................................... 15
3.1 Software guide ................................................................................................................................................................................16
3.1.1 Requirements .............................................................................................................................................................................16
3.1.2 Installation..................................................................................................................................................................................16
3.1.3 Software Operating Procedure ....................................................................................................................................................19
3.1.4 Graphical User Interface..............................................................................................................................................................20
3.1.5 eONE Controls.............................................................................................................................................................................21
3.1.6 Digital Offset Compensation........................................................................................................................................................26
3.1.7 Statistic Data...............................................................................................................................................................................27
3.1.8 Trace Visualization ......................................................................................................................................................................28
3.1.9 Keiboard shortcuts......................................................................................................................................................................29
3.1.10 Save & Read Data ...................................................................................................................................................................29
Troubleshooting guide....................................................................................................................................................................................... 35
Technical support & repairs............................................................................................................................................................................... 36

1
elements
enabling technologies for Life Science
eONE - User’s guide
SECTION 1
1 Preliminary Information
General notes.
Trademarks.
Instructions on disposal.
Description of safety symbols.
Qualified Personnel.
Basic knowledge required.
Proper use of the product.
Purpose of the user’s guide.
Scope of the operating instructions.
The manual is a part of the system.
Figures.
Safety instructions.
Notes about usage.

2
elements
enabling technologies for Life Science
SECTION 1 –Preliminary Information
IT
EN
FR
1.1 General notes
a) The information in this manual is subject to change and is in no way
binding upon ELEMENTS s.r.l.
b) ELEMENTS s.r.l. is not responsible for technical errors or other omissions
in the manual, and shall not accept any responsibility deriving from its
use.
1.2 Trademarks
a) All brands and product names mentioned in this manual are trademarks
of their respective owners.
1.3 Instructions on disposal
Il simbolo sul prodotto o sulla confezione indica che il prodotto non
deve essere considerato come un normale rifiuto domestico, ma deve
essere portato nel punto di raccolta appropriato per il riciclaggio di ap-
parecchiature elettriche ed elettroniche. Provvedendo a smaltire questo
prodotto in modo appropriato, si contribuisce a evitare potenziali conse-
guenze negative per l’ambiente e la salute, che potrebbero derivare da
uno smaltimento inadeguato del prodotto. Per informazioni più detta-
gliate sul riciclaggio di questo prodotto, contattare l’ufficio comunale, il
servizio locale di smaltimento rifiuti o il fornitore da cui è stato acquista-
to il prodotto.
The symbol on the product or in its packaging indicates that this
product may not be treated as household waste. Instead it shall be
handed over the applicable collection point for the recycling of electrical
and electronic equipment. By ensuring this product is disposed of cor-
rectly, you will help prevent potential negative consequences for the en-
vironment and human health, which could otherwise be caused by inap-
propriate waste handling of this product. For more detailed information
about recycling of this product, please contact your local city office, your
household waste disposal service or the supplier where you purchased
the product.
Le symbole sur le produit ou son emballage indique que ce produit
ne peut être traitè comme décher ménager. It doit être remis au point
de collecte dèdié à cet effect (collect et recyclage du matèriel èlectrique
et èlectronique). En procèdant à la mise à la casse règlementaire de
l’appareil, nous prèservons l’environnement et notre sécurité, s’assurant
ainsi que les dèchets seront traitès dans des conditions appropriées.
Pour obtenir plus de dètails sur le recyclage de ce produit, veuillez
prendre contact avec les services de votre commune ou le distributeur
où vous avez effectué l’achat.

3
elements
enabling technologies for Life Science
eONE - User’s guide
DE
ES
PT
Table 1
Safety symbols
Das Symbol auf dem Produkt oder seiner Verpackung weist darauf
hin, dass dieses Produkt nicht als normaler Haushaltsabfall zu behandeln
ist, sondern an einem Sammelpunkt für das Recycling von elektrischen
und elektronischen Geräten abgegeben werden muss. Durch ihren Bei-
trag zum korrekten Entsorgen dieses Produkts schützen Sie die Umwelt
und die Gesundheit Ihrer Mitmenschen. Umwelt und Gesundheit
werden durch falsches Entsorgen gefährdet. Weitere Informationen über
das Recycling dieses Produkts erhalten Sie von Ihrem Rathaus, Ihrer Mül-
labfuhr oder den Distributoren, in dem Sie das Produkt gekauft haben.
El simbolo en el producto o en su embalaje indica que este produc-
to no se puede tratar como desperdicios normales del hogar. Este pro-
ducto se debe entregar al punto de recolección de equipos eléctricos y
electrónicos para reciclaje. Al asegurarse de que este producto se dese-
che correctamente, usted ayudará a evitar posibles consequencias nega-
tivas para el ambiente y la salud pública, lo qual podria ocurrir si este
producto no se manípula de forma adecuada. Para obtener informació-
nes mas detalladas sobre el reciclaje de este producto, póngase en con-
tacto con la adMinistraciòn de su ciudad, con su servicio de desechos del
hogar o con el surtidor donde comprò el producto.
O simbolo no produto ou na embalagem indica que este producto
não pode ser tratado como lixo doméstico. Em vez disso, deve ser entre-
gueado ao centro de recolha selectiva para a reciclagem de equipamen-
to electrico e electronico. Ao garantir uma eliminação adequada deste
produto, ira ajudar a evitar eventuais consequencjas negativas para o
meio ambiente e para a saude publica, que, de outra forma, poderiam
ser provocadas por un tratamento incorrecto do produto. Para obtener
informações mais detalhadas sobre a reciclagem deste produto, contac-
te os serviços municipalizados locais, o centro de recolha selectiva da
sua area de residência ou no distribuidor onde adquirir ou produto.
1.4 Description of safety symbols
Danger
This symbol indicates a danger to life or health of personnel.
Warning
This symbol indicates a danger to the hardware and / or the en-
vironment.
Note
This symbol indicates an additional information meant to provide
a better understanding.
ESD
This symbol indicates to pay attention and observe precautions
for handling electrostatic sensitive devices

4
elements
enabling technologies for Life Science
SECTION 1 –Preliminary Information
1.5 Qualified Personnel
a) eONE may be operated only by personnel qualified for the specific task
in accordance with the relevant documentation for the specific task, in
particular its warning notices and safety instructions.
b) Qualified personnel are those who, based on their training and experi-
ence, are able to identify risks and avoid potential hazards when working
with these systems.
1.6 Basic knowledge required
a) To understand operating instructions a general knowledge of infor-
mation technology is needed.
b) Knowledge of personal computers and the Microsoft o.s. is required to
understand this user’s guide.
1.7 Proper use of the product
a) ELEMENTS s.r.l. products may only be used for the applications described
in the catalogue and in the technical documentation.
b) If products and components from other manufacturers are used, these
must be approved by ELEMENTS S.R.L..
c) Proper transport, assembly, installation, storage, commissioning, opera-
tion and maintenance are required to ensure that the product operates
safely.
d) The indicated environmental conditions must be observed.
e) The information in this user’s manual must be observed.
1.8 Purpose of the user’s guide
a) This user’s manual contains information based on the requirements de-
fined by DIN EN 62079 for mechanical engineering documentation.
b) These operating instructions are intended for:
1. Users.
2. Commissioning engineers.
3. Maintenance personnel.
c) Pay attention at the information in the chapter "Safety instructions".
d) More information such as operating instructions, examples and refer-
ence information, are available in the online help of Premium HMI soft-
ware and Ubiquity software.

5
elements
enabling technologies for Life Science
eONE - User’s guide
1.9 Scope of the operating instructions
a) The operating instructions apply to the eONE family.
1.10 The manual is a part of the system
b) This user’s guide belongs to eONE and is also required for commission-
ing.
c) Keep all supplied documentation for the entire service life of eONE.
1.11 Figures
a) This manual contains illustrations of the described devices.
b) Some details of the illustrations may differ from the device provided.

6
elements
enabling technologies for Life Science
SECTION 1 –Preliminary Information
Warning
ESD
1.12 Safety instructions
1.12.1 Installation according to the instructions
Commissioning the eONE device is prohibited until it has been absolutely
ensured that the system in which the eONE device is to be installed
complies with all the applicable EU and international regulation.
1.13 Notes about usage
eONE is approved for indoor use only.
eONE may be damaged if operated outdoors.
eONE may be Static Sensitive. Observe precautions for handling electro-
static sensitive devices.
eONE may be affected by humidity. If used in a high humidity environ-
ment, it can take longer time to stabilize to standard performances once
connected.

7
elements
enabling technologies for Life Science
eONE - User’s guide
SECTION 2
2 General description and connections
Product description.
Key features.
Package.
Hardware Setup.
Front Connectors.
Rear Connectors.
Preparation for installation.
Select the location.
Checking the package contents.
Checking the operation conditions.
Typical Experimental Setup.

8
elements
enabling technologies for Life Science
SECTION 2 –General description and connections
Figure 1
eONE detail
2.1 Product description
eONE (Miniaturized One-Channel Amplifier) is a new current measurement sys-
tem entirely contained in a USB stick, thanks to a custom-made microchip for
low-noise current signal acquisitions.
2.2 Key features
•Two input current ranges: ±200 pA and ±20 nA;
•Eight selectable sampling rates: 1.25 kHz, 5 kHz, 10 kHz, 20 kHz, 40 kHz,
50 kHz, 100 kHz and 200 kHz;
•Very low noise: ≈100 fA rms at 1 kHz, ≈380 fA rms at 10 kHz & ≈3,2 pA
rms at 100 kHz (open input);
•Software controlled voltage stimulus with 1mV resolution;

9
elements
enabling technologies for Life Science
eONE - User’s guide
Table 2
Package
2.3 Package
eONE package consists of:
eONE
Software interface
SMB plug adaptor
USB cable
Ground cable

10
elements
enabling technologies for Life Science
SECTION 2 –General description and connections
Figure 2
Front connectors
2.4 Hardware setup
2.4.1 Front Connections
eONE is designed to be as simple as possible to connect to your different set-
ups.
In order to obtain the best performance, the main things to be careful on are
parasitic capacitances on the input and correct shielding of the overall hard-
ware setup to avoid the 50/60Hz line interferences.
The following paragraphs describe all the connections and how to connect them
in the right way.
Input
Reference
On the front of eONE there is the connection for your Device Under Test (DUT).
This connector is a coaxial SMB male gold plated connector where the center
contact is the current measurement input and the outer contact is the refer-
ence voltage.
2
1
1
2

11
elements
enabling technologies for Life Science
eONE - User’s guide
Figure 3
Rear Connections
2.4.2 Rear Connections
USB
LED
Ground
On the rear of eONE there are two connectors:
-mini-B female USB connector to connect to the PC through the in-
cluded USB cable;
-2mm gold plated ground female connector to connect the eONE
ground to the Faraday cage;
3
2
1
3
2
1

12
elements
enabling technologies for Life Science
SECTION 2 –General description and connections
Note:
please refer to paragraph 2.3 “Package”.
2.4.3 Select the location
The mounting location should comply with the following characteristics:
a) Avoid direct sunlight exposure.
b) Make sure that eONE is properly (ergonomically) accessible to the op-
erator.
c) Choose a suitable mounting height.
2.4.4 Checking the package contents
Check the package content for visible signs of transport damage and
for completeness.
In the case of damaged parts, contact your ELEMENTS S.R.L. repre-
sentative. Do not install parts damaged during shipment.
2.4.5 Checking the operating conditions
Read carefully the standards, approvals, EMC parameters and technical
specifications for operation of the device.
Follow the instructions for local use of the device: Notes about usage.

13
elements
enabling technologies for Life Science
eONE - User’s guide
Figure 4
Typical Experimental Setup
Warning:
Avoid short circuits between the SMB plug
adaptor and the Faraday cage
Note:
Avoid the use of coaxial cables to connect
the DUT to the front connector
Figure 5
Real Setup Example
2.4.6 Tipical Experimental Setup
This picture shows how to correctly connect eONE in a typical electrophysiology
setup:
To obtain the best noise performances a particular care must be given to the
shielding with a metallic cage (Faraday cage) all around the experimental setup.
In particular, it is important to connect the Faraday cage to the ground con-
nector placed on the rear of eONE.
In order to minimize the noise it is also important to minimize parasitic capaci-
tances between the input and the shielding by keeping the wires as short as
possible and the farthest as possible from the Faraday cage, including your De-
vice Under Test (DUT).
With certain PC, there can be some issues with USB power supply that can in-
troduce noise and peaks on the acquired signal. In these cases we recommend
to use a powered USB 2.0 hub.
This is an example of a real setup for ion-channel studies on bilayer lipid mem-
branes:

14
elements
enabling technologies for Life Science
SECTION 2 –General description and connections

15
elements
enabling technologies for Life Science
eONE - User’s guide
SECTION 3
3 Software installation and description
Software guide.
Requirements.
Installation.
Software Operation Guide.
Graphical User Interface.
eONE Controls.
Digital Offset Compensation.
Statistic Data.
Trace Visualization.
Save Data.

16
elements
enabling technologies for Life Science
SECTION 3 –Software installation and description
Warning
IMPORTANT: The software must be in-
stalled BEFORE connecting the eONE device
to the PC
Figure 6
Elements Data Reader Install Program
3.1 Software guide
Elements Data Reader (EDR) is the control software for eONE devices.
It enables the total control of eONE devices features (such as set the data acquisi-
tion properties and the control voltage) and enables the real time data visualiza-
tion and data saving.
Data acquired by means of this custom software are handily readable by Matlab®
and can be saved to be compatible with standard commercial software such as
pClamp® from Molecular Devices® or Origin® from OriginLab®.
3.1.1 Requirements
The minimum system requirements are:
Dual core processor 1GHz or better
1GB of RAM
A free USB 2.0 port
Windows XP / Vista / Seven / 8
3.1.2 Installation
To start the installation procedure:
double click on the installation executable EDR_setup_X.XX.exe and fol-
low the instructions.