Fisher Bioblock Scientific Stemi 1000 User manual

12
Stemi 1000/2000/2000-C
Stereo Microscopes
Operating Manual

Unless expressly authorized, forwarding and duplication of this document, and the
utilization and communication of its contents are not permitted. Violations will entail an
obligation to pay compensation.
Number of this
manual: B 40-002 e
Date of issue: 12/98

Issued by: Carl Zeiss
Mikroskopie
D-07740 Jena
Telephone (**49) 03641 / 64-1616
Telefax: (**49) 03641 / 64-3144
Internet: [email protected]
http://www.zeiss.de
Knowledge of this manual is required for the operation of the instrument. Would you therefore
please make yourself familiar with the contents of this manual and pay special attention to hints
concerning the safe operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
All rights reserved in the event of granting of patents or registration of a utility model.

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 I
TABLE OF CONTENTS
Page
Title
Copyright
Table of contents I. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
List of figures III. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Note IV. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
General view V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Notes on device safety VII. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1 DESCRIPTION
1.1 Designation, purpose 1-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.2 Description of the units 1-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.3 Technical data 1-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.4 Overview of modules 1-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
1.5 Function elements 1-29. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2 OPERATION
2.1 Unpacking and setting up 2-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2 Commissioning 2-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2.2.1 Working with accessories 2-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3 FLUORESCENCE CONTRAST WITH STEREO MICROSCOPES
3.1 FL S (Fluorescence for Stereo microscope) configuration 3-2. . . . . . . . . . . . . . . . . .
3.1.1 Name and intended application 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2 Configurations 3-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2.1 FL S configuration with KL 1500 electronic (not suitable for UV excitation) 3-4. . .
3.2.2 FL S configuration with HBO 50 or HBO 100 3-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3.2.3 FL S configuration with LUMATEC HBO 200 3-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
II
4 CARE AND TROUBLESHOOTING
4.1 Care 4-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4.2 Troubleshooting and service 4-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Page
ANNEX
– List of abbreviations A-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
– Certification in accordance with DIN ISO 9001/EN 29001/EN 46001 A-5. . . . . . . .
– EC conformity declaration A-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 III
LIST OF FIGURES Page
Figure 1-1 Principal assemblies of the Stemi 2000-C 1-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-2 Optical schematic of the Stemi 2000-C 1-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-3 Basic microscope equipment 1-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-4 Eyepieces/eyepiece cups/reticules/attachment systems/specimen
measuring plate 1-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-5 Stands 1-12. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-6 Specimen stages 1-14. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-7 Halogen lighting systems for vertical illumination 1-16. . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-8 Cold light sources for vertical illumination 1-18. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-9 Halogen lighting systems for transmitted illumination 1-20. . . . . . . . . . . . . . . . . . . . . .
Figure 1-10 Cold light sources for transmitted illumination 1-22. . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-11 Polarisation systems 1-24. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-12 Documentation systems using photo cameras 1-26. . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-13 Adapters for video cameras 1-28. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 1-14 Function elements of the Stemi 2000-C 1-30. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-1 Stemi 2000-C packing unit in city box 2-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-2 Unpacking 2-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-3 Setting up the Stemi 2000-C 2-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-4 Adjusting the Stemi 2000-C 2-3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-5 Inserting a graticule 2-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-6 Fitting an attachment system to the Stemi 2000 2-4. . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-7 Mounting the polariser 2-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-8 Mounting a reflex camera 2-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 2-9 Mounting the microscope camera MC 80 for 35 mm photography 2-7. . . . . . . . . .
Figure 2-10 Mounting a video camera 2-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
IV
Page
Figure 3-1 Stemi 2000 with KL 1500 electronic 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-2 Stemi SV 6 with HBO 100 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-3 Stemi 2000 with HBO 50 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-4 Stemi SV 11 with LUMATEC HBO 200 3-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-5 FL S configuration with KL 1500 electronic 3-4. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-6 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-7 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-8 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-9 3-5. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-10 FL S configuration with HBO 50 or HBO 100 3-6. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-11 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-12 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-13 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-14 3-7. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-15 FL S configuration with LUMATEC HBO 200 3-8. . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-16 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-17 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-18 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-19 3-9. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-20 3-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 3-21 3-10. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Figure 4-1 Lamp replacement 4-2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 V
NOTE
•The figures integrated in the text each have a figure number and a caption, e.g. ”Fig. 2-8”signifies:
the figure in Section 2 with the serial number 8. In each figure, details discussed in the text are
assigned a reference line marking and an item number. In the running text, ”T2 adapter (2-8/3)”: in
Fig. 8 of Section 2, the T2 adapter is marked with the item number 3.
•Refer to the annex for explanations of the abbreviations.
•This instruction manual refers to the Stemi 1000, Stemi 2000 or the Stemi 2000-C microscope
versions (see Page 1-9) and their options.
Apply this manual analogously when working with other variants of the unit.

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
VI
GENERAL VIEW
(Basic unit)
Stemi 1000 Stemi 2000
Stemi 2000-C

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 VII
NEW MODEL
We have included a further Stemi model in our line of Greenough-type stereo microscopes.
In response to many requests received from customers, the
Stemi 2000-CS 45 50 55
is now also available as a brand-new item in addition to the Greenough-type stereo microscopes
Stemi 1000, Stemi 2000 and Stemi 2000-C.
Unlike the Stemi 2000-C, the Stemi 2000-CS (”C”for ”Camera”and ”S”for ”Splitter”) features a
fixed camera port
with a 50/50 % beam splitting ratio, i. e. the camera port has no switching lever.
Unlike the Stemi 2000-C, the Stemi 2000-CS therefore provides the benefit of
simultaneousstereoscopic viewing
while taking photographs or working in the monitor mode.
All configurations or accessory components are identical to those of the other Stemi’s.

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
VIII
NOTES OF DEVICE SAFETY
The Stemi 1000, Stemi 2000 or the Stemi 2000-C stereo microscope including original accessories or
options must only be used for the microscopy applications described in this operating manual.
The manufacturer cannot assume any liability for any other applications, possibly also involving indi-
vidual modules or single parts. This also applies to all service or repair work that is not carried out by
authorised service personnel. All guarantee/warranty claims also expire for all those parts that were not
directly affected by repair.
Particular attention must be paid to the following warning notes:
The Stemi was designed and tested in conformity with the IEC Publication 1010-1 ”Safety
requirements for electrical measuring devices, control equipment and laboratory apparatus”and
was delivered in a safe state. This operating manual contains information and warnings which must
be followed by the operator.
The Stemi is a light microscope conceived in accordance with the latest scientific and technical
knowledge for visual, microphotographic and video analysis of microscopic specimens. The unit
must only be used for the intended purpose. It is not intended for continuous unsupervised opera-
tion!
The Stemi has no special facilities to protect against samples that have caustic, toxic, radioactive
or other effects that are damaging to health.
Users must check whether the available power supply agrees with the value specified on the rating
plate of the respective power supply unit.
The power supply unit belonging to the Stemi is a protective class II unit.
Internal parts can carry hazardous voltages when a power supply unit is connected to the mains.
The power supply unit must therefore be disconnected from the mains before opening it.
Make sure that only those fuses are used as spares for the respective power supply unit that are
actually intended for the prescribed rated current and the specified version. It is forbidden to use
provisional fuses or to short-circuit the fuse holders.
Modifications to the unit to keep in line with technical progress are always reserved.

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 1-1
1 Description
1.1 Designation, purpose
Manufacturer’s designation: stereo microscope Stemi 1000
stereo microscope Stemi 2000
stereo microscope Stemi 2000-C
stereo microscope Stemi 2000-CS
Brief designation: Stemi 1000
Stemi 2000
Stemi 2000-C
Stemi 2000-CS
Within the product family of stereo microscopes from Carl Zeiss, these units fit in as follows:
•Greenough type: Stemi D •Telescopic type: Stemi SV 6
Stemi DRC Stemi SV 11
Stemi SV 11 Apo
Stemi 1000
Stemi 2000
Stemi 2000-C
Stemi 2000-CS
Stemi 1000 and Stemi 2000 are universally applicable Greenough type stereo microscopes that unite
a high imaging quality, compact ergonomic design and internationally standardised connection and
coupling points.
While the Stemi 1000 and Stemi 2000 are units for visual stereoscopic observation, with its trinocular
tube the Stemi 2000-C offers all possibilities of micrographic and video image documentation.
Unlike the Stemi 2000-C, the Stemi 2000-CS features a fixed camera port with a 50/50 % beam splitting
ratio, i. e. the camera port has no switching lever. Unlike the Stemi 2000-C, the Stemi 2000-CS therefore
provides the benefit of simultaneous stereoscopic viewing while taking photographs or working in the
monitor mode.
Both stereo microscopes’areas of use cover the following focal areas, among other things:
•Materials technology and sciences
•Biotechnology and medical technology
•Semiconductors industry and research
•Hobby and leisure
IMPORTANT The units may only be used for the work and applications described in this manual. Refer
also to the product liability declaration (Page VIII).

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
1-2
1.2 Description of the units
The Stemi 1000, Stemi 2000 and Stemi 2000-C are highly powerful stereo microscopes that are dis-
tinguished by compact and ergonomic design.
Figure 1-1 shows the principal assemblies of the Stemi 2000-C.
•Stand S with 260 mm column (1-1/4)
•Stemi mount with drive for column 32 (1-1/6)
•Stemi 2000-C microscope body (1-1/2)
•Eyepieces W-PL 10x/23 Spec. foc. (1-1/1) with eye cup
•Built-in 6V, 10 W halogen vertical illumination (1-1/5) with 115/230 V –6 V 50 VA power supply (1-1/3)
Figure 1-1 Principal assemblies of the Stemi 2000-C
3
1
4
2
5
6

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 1-3
Figure 1-2 schematically shows the optical beam path in the Stemi 2000-C.
The unit is switched over between the right tube connector and the camera output with the aid of an
adjustable reflector group located under the tube system that features 100/0 % and 0/100 % intensities.
Figure 1-2 Optical schematic of the Stemi 2000-C

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
1-4
1.3 Technical data
(1) Dimensions and weight
Mikroscope
•Stemi 1000
Dimensions (width x depth x height) 65 x 336 x 371 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight (with power supply, mains cable and lamp) 4.33 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
•Stemi 2000
Dimensions (width x depth x height) 65 x 336 x 371 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight (with power supply, mains cable and lamp) 4.53 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
•Stemi 2000-C
Dimensions (width x depth x height) 65 x 336 x 371 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight (with power supply, mains cable and lamp) 4.8 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
•Stand S
Support area (width x depth) 180 x 270 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight 1.3 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
•Stand S and power supply unit 230 V - 6 V 50 VA
Support area (width x depth) 263 x 270 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight 1.96 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
City Box
Dimensions (closed) (width x depth x height) 560 x 450 x 240 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Weight (unequipped) 4.75 kg. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(2) Ambient conditions
Storage and transport (in packaging)
Permissible ambient temperature -40 to +70 °C. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible relative humidity v100 %. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible atmospheric pressure 500 to 1060 mbar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible sinusoidal oscillations 10 to 150 Hz; 0.5 g. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible impact (handling impact) 30 g; 6 ms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 1-5
Operation
Permissible ambient temperature +15 to +35 °C. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible relative humidity v85 %. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible atmospheric pressure 800 to 1060 mbar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible impact (handling impact without 10 g; 6 ms; 100 impacts in operating. . . . . . . . . . . . . . . . .
unit packaging) position
(3) Operating data
230 V – 6 V 50 VA power supply (Order No. 458420)
120 V – 6 V 50 VA power supply (Order No. 458421)
Mains voltage 120 V or 230 V. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible mains voltage fluctuation +6/-10 %. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Mains frequency 50/60 Hz. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Power consumption max. 50 VA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Light source halogen 6 V 10 W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Variation range 2.4 ... 5.8 V continuously. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Class of protection II. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Electrical degree of protection IP 20. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Electric safety in acc. IEC 1010-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
RFI suppression in acc. EN 55011 Class B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Power supply unit, stabilised 6 V 20 W, 115 V – 230 V, variable 1.5 ... 6 V, 50 ... 60 Hz, 40 VA
(Order No. 458415)
Mains voltage 100 V, 115 V, 220 V, 240 V, switchable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Permissible mains voltage fluctuation "10 %. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Mains frequency 50 ... 60 Hz. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Power consumption 40 VA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Light source halogen 6 V 20 W. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Variation range 1.5 ... 6.0 V continuously. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Class of protection I. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Electrical degree of protection IP 40. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Electric safety in acc. IEC 1010-1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
RFI suppression in acc. EN 55011 Class B. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
1-6
Specimen stages, adjustment ranges
Sliding stage "20 mm, rotatable by 360°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Spherical stage "30°horizontal tilt angle, rotatable by 360°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Rotary stage rotatable by 360°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Specimen guide D 28 x 75 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Expandable stage with specimen guide and holding frame 76 x 50 mm. . . . . . . . . . . . . . . . . . . . . . . . . .
Stage inlay plates
Outer diameter 84 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Outer diameter (rotary stage) 72 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Stemi mount with drive for column 32
Focusing by means of focusing drive 46 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Mount for microscope body ∅76 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
(4) Optical and mechanical data
Stemi 1000 microscope body
Effective principle according to GREENOUGH with optical compensation. . . . . . . . . . . . . . . . . . . . . . . .
Stereo angle 11°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Zoom range of the pancratic lens 0.7x ... 3.5x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Free working distance (without dust glass) 112 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Viewing angle 35°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Adjustable eye distance 55 to 75 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Mount diameter 76 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Stemi 2000/2000-C microscope body
Effective principle according to GREENOUGH with mechanical compensation. . . . . . . . . . . . . . . . . . . .
Stereo angle 11°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Zoom range of the pancratic lens 0.65x ... 5.0x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Free working distance (without dust glass) 92 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Viewing angle 35°. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 1-7
Adjustable eye distance 55 to 75 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Mount diameter 76 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Replaceable optical systems
Eyepieces W-PL 10x/23 Br. spect. foc.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . W 10x/21 foc.
W-PL 16x/16 Br. spect. foc.
W 25x/10 foc.
Attachment systems, fixed ’= 0.3x FOD = 285 mm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ’= 0.4x FOD = 210 mm
’= 0.63x FOD = 130 mm
’= 2.0x FOD = 31 mm
Stemi 1000 visual viewing magnification ranges
Basic microscope equipment 7x ... 35x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
with replaceable optical system 2,1x ... 175x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Stemi 2000/2000-C visual viewing magnification ranges
Basic microscope equipment 6.5x ... 50x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
with replaceable optical system 1.95x ... 250x. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
VSS Okular
W 10x/21 foc. W-PL 10x/23 spect. foc. W-PL 16x/16 spect. foc. W 25x/10 foc.
Total
magnification Specimen field (mm) Total magnification Specimen field (mm) Total magnification Specimen field (mm) Total magnification Specimen field (mm)
0.3x 285 2.1x...10.5x 100.0...20.0 2.1x...10.5x 109.5...21.9 3.4x...16.8x 76.2...15.2 5.3x...26.3x 47.6...9.5
1.95x ... 15.0x 107.7...14 1.95x... 15.0x 118...15.3 3.1x...24.0x 82.1...10.7 4.9x...37.5x 51.3...6.7
0.4x 210 2.8x...14.0x 75.0...15.0 2.8x...14.0x 82.1...16.4 4.5x...22.4x 57.1...11.4 7.0x...35.0x 35.7...7.1
2.6x...20.0x 80.8...10.5 2.6x...20.0x 88.5...11.5 4.2x...32.0x 61.5...8.0 6.5x...50.0x 38.5...5.0
0.63x 130 4.4x...22.1x 47.7x...9.5 4.4x...22.1x 52.2...10.4 7.1x...35.3x 36.3...7.3 11.0x...55.1x 22.7...4.5
4.1x...31.5x 51.3...6.7 4.1x...31.5x 56.2...7.3 6.6x...50.4x 39.1...5.1 10.2x...78.8x 24.4...3.2
1x 112 7.0x...35.0x 30.0...6.0 7.0x...35.0x 32.9...6.6 11.2x...56.0x 22.9...4.6 17.5x...87.5x 14.2...2.9
92 6.5x...50x 32.3...4.2 6.5x...50x 35.4...4.6 10.4x...80.0x 24.6...3.2 16.3x...125.0x 15.4...2.0
2x 31 14.0x...70.0x 15.0...3.0 14.0x...70.0x 16.4...3.3 22.4x...112.0x 11.4...2.3 35.0x...175.0x 7.1...1.4
13x...100.0x 16.2...2.1 13x...100.0x 17.7...2.3 20.8x... 60x 12.3...1.6 32.5x...250x 7.7...1.0
Stemi 2000 Stemi 1000
Microphotographic magnification scales for MC 80 (24 x 36 mm miniature format)
Basic microscope equipment from 1.62:1 to 12.5:1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
with replaceable optical system from 0.49:1 to 25:1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
1-8
1.4 Overview of modules
Basic microscope equipment
Figure 1-3 Basic microscope equipment
1
1.1
1.3
1.4
1.2
1.5
2
2.4
2.1
2.5
2.6
2.2
2.7
2.3
3
3.4
3.5
3.1
3.7
3.2
3.3
3.6

Stemi 1000, Stemi 2000, Stemi 2000-C
B 40-002 e 12/98 1-9
Microscope equipment Order No. Stemi
1000 Stemi 2000 Stemi 2000-C
1Stereo microscope Stemi 1000
consisting of 
1.1 •Stemi 1000 microscope body 455050 
1.2 •Eyepiece W 10x/21 foc. 2x 455042   
-•Attachment systems adapter (contained in 455050) -
1.3 •Stemi mount with drive for column 32 455094   
1.4 •Stand S, with column 32, 260 mm long 455104   
1.5 •Dust cover K 459300   
2Stereo microscope Stemi 2000 with built-in 6 V,
10 W halogen vertical illumination (230 V)
consisting of
495102 9804 
2.1 •Stemi 2000 microscope body 455052 
2.2 •Eyepiece W-PL 10x/23 spectacle focusing 2x 455043   
2.3 •Eyepiece cup 2x 444801   
2.4 •Stemi mount with drive for column 32 455094   
2.5 •Stand S with column 32, 260 mm long 455104   
2.6 •Built-in 6 V, 10 W halogen vertical illumination
(230 V) 485001 9804  
2.7 •Dust cover K 459300   
not
illus. Stereo microscope Stemi 2000 with built-in 6 V, 10 W halogen vertical illumination (120 V) 495102 9904 
3Stereo microscope Stemi 2000-C with built-in 6 V, 10
W halogen vertical illumination (230 V)
consisting of
495104 9804 
3.1 •Stemi 2000-C microscope body 455053 
3.2 •Eyepiece W-PL 10x/23 spectacle focusing 2x 455043   
3.3 •Eyepiece cup 2x 444801   
3.4 •Stemi mount with drive for column 32 455094   
3.5 •Stand S with column 32, 260 mm long 455104   
3.6 •Built-in 6 V, 10 W halogen vertical illumination
(230 V) 485001 9804  
3.7 •Dust cover K 459300   
not
illus. Stereo microscope Stemi 2000-C with built-in 6 V, 10 W halogen vertical illumination (120 V)
but with:
495104 9904 
•120 V –6 V 50 VA power supply with mains cable
and American flat plug 458421  
not
illus. Stereo microscope Stemi 2000-CS with stand N and universal vertical illumination
(230 V)
(115 V) 000000 1006 130
000000 1006 132
 Part of the microscope equipment
Unit capable of functioning in conjunction with the microscope equipment

Stemi 1000, Stemi 2000, Stemi 2000-C
12/98 B 40-002 e
1-10
Accessories
(1) Replaceable optical systems
Figure 1-4 Eyepieces/eyepiece cups/reticules/attachment systems/specimen measuring plate
7 68
768
3
4
5
6
7
2
2
3
3
4
4
5
5
6
6
7
7
8
8
0 5 10 15 20 25
12345
mm
5
4
6
4
3
3.1
3.2
3.3
3.4
3.5
3.6
6.1
36.2 6.3 6.4 6.5
4.1
4.2
4.3
11.1 1.2 1.3 1.4
22.1 2.1
5.1