Nikon Eclipse Ni-U User manual

Specifications
Maintenance and
Storage
Troubleshooting
Assembly
Upright Microscope
Instruction Manual
Assembly/Maintenance
M570 E 11.8.NF.1 (2/2)

Introduction
i
Introduction
Thank you for purchasing a Nikon product.
This instruction manual is written for users of the Nikon ECLIPSE Ni-U microscope. To ensure correct usage, read this
manual carefully before operating this product.
●No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.
●The contents of this manual are subject to change without notice.
●The equipment described in this manual may differ from the actual product in its appearance.
●Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If
you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
●Some of the equipment described in this manual may not be included in the set you have purchased.
●If you intend to use any other equipment with this product, read the manual for that equipment too.
●If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may
be impaired.
●Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly
contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your
opinion.

Introduction
ii
Contents of the Manual
The instruction manual for ECLIPSE Ni-U is provided as two volumes.
Operation
Safety Precautions
Components
Microscopy Procedures
Operation Flowchart
Bright/Dark-field Microscopy
Epi-fluorescence Microscopy
Differential Interference Contrast Microscopy
Phase Contrast Microscopy
Motorized Bright-field Microscopy
Motorized Epi-fluorescence Microscopy
Individual Operations
Assembly/Maintenance (This manual)
Assembly
Troubleshooting
Maintenance and Storage
Specifications
Before reading the “Assembly/Maintenance” manual,
read the “Safety Precautions” in the “Operation”
manual.
Symbols Used in This Manual
The following symbols are used in this manual.
Symbols for Safety
WARNING
CAUTION
Highlights important information that should be noted for safety. Read “Safety Precautions” for details.
Other Symbols
Indicates information you should note or comply with to prevent defects or malfunction of this product.
Indicates information you should be aware of in using this product, as well as other useful information.

Summary of Contents
iii
SpecificationsMaintenance
and Stora
g
e
Troubleshooting
Assembly
Summary of Contents (See the next page for the detailed contents.)
Introduction
Contents of the Manual
Symbols Used in This Manual
Assembly
Troubleshooting
Maintenance and Storage
Specifications

Contents
iv
Contents
Introduction .........................................................................................................................i
Contents of the Manual ....................................................................................................ii
Symbols Used in This Manual ..........................................................................................ii
Summary of Contents ....................................................................................................... iii
Assembly.................................................................................................. 1
1 ECLIPSE Ni-U System Configuration .......................................................................2
2 Components List .......................................................................................................3
3 Assembly Method......................................................................................................4
Introduction ...................................................................................................................... 4
[1] Check the input voltage...................................................................................... 4
[2] Attach the DIA motorized shutter (optional). ...................................................... 5
[3] Attach the lamp. ................................................................................................. 5
[4] Attach the lamphouse for dia-illumination. ......................................................... 5
[5] Attach the standard arm/contact arm. ................................................................ 6
[6] Attach the epi-fluorescence cube turret and epi-fluorescence attachment
(required for epi-fluorescence microscopy)........................................................ 7
■Attaching a filter cube ............................................................................... 8
■Replacing excitation and barrier filters ..................................................... 9
■Attaching a light shielding plate................................................................ 9
[7] Attach the quadrocular tilting tube and DSC zooming port
for quadrocular tube. ........................................................................................ 10
[8] Attach the EPI motorized shutter (optional). .................................................... 10
[9] Attach eyepieces. ..............................................................................................11
[10] Attach the substage...........................................................................................11
[11] Attach the condenser holder. ........................................................................... 12
[12] Attach the stage. .............................................................................................. 12
[12.1] For standard stage .......................................................................... 12
[12.2] For rotatable ceramic coated stage................................................. 12
[13] Attach the condenser........................................................................................ 13
■Attaching the optical module to the universal condenser....................... 13
■Attaching the DIC module to DIC condenser oil..................................... 17
[14] Attach the nosepiece........................................................................................ 19
[15] Attach the objective. ......................................................................................... 19
[16] Attach the DIC rotation polarizer unit (required for differential interference
contrast microscopy). ....................................................................................... 19
[17] Attach the eyelevel riser (optional)................................................................... 20
[18] Attach a camera and connect the DS camera control unit (optional). ............. 20
[18.1] When attaching to the DSC port for the ergonomic binocular tube 20
[18.2] When attaching to the DSC zooming port for quadrocular tube ..... 21
[18.3] When attaching to the quadrocular tilting tube................................ 21
[18.4] When attaching a camera to the trinocular tube ............................. 21
[19] Replace the ND filter (optional)........................................................................ 22
[20] Connect the motorized device cable................................................................ 22
[21] Connect the power cord. .................................................................................. 24
Troubleshooting..................................................................................... 25
1 Optical System and Operation ................................................................................26
1.1 General............................................................................................................. 26
1.2 Epi-fluorescence Microscopy ........................................................................... 30
1.3 Differential Interference Contrast Microscopy.................................................. 31

Contents
v
1.4 Phase Contrast Microscopy ............................................................................. 32
2 Electrical Requirements ..........................................................................................33
2.1 General............................................................................................................. 33
2.2 Epi-fluorescence Microscopy ........................................................................... 34
Maintenance and Storage ..................................................................... 35
1 Replacing the Lamp ................................................................................................36
2 Cleaning ..................................................................................................................37
2.1 Cleaning Lenses............................................................................................... 37
2.2 Cleaning Parts Other than the Lens................................................................. 37
2.3 Cleaning Immersion Oil .................................................................................... 38
2.4 Decontaminating the Product........................................................................... 38
3 Transportation .........................................................................................................38
4 Storage....................................................................................................................38
5 Regular Inspections (Charged) ...............................................................................38
Specifications ........................................................................................ 39
1 Microscopy (Principles) ...........................................................................................40
2 Performance Properties ..........................................................................................40
3 Physical Properties .................................................................................................43

Contents
vi

1
Assembly
1
Chapter
1
Assembly
This chapter contains an Ni-U system configuration diagram, a list of its components, and explains how to assemble the
system.
Read appropriate notes such as CAUTION “9 Cautions on assembling and installing the product” at the beginning of
the separately provided instruction manual “Operation” or “4 Installation location” in “Notes on Handling the Product” in the
same manual before you work on assembling.
■Assembly tools provided with the microscope
•Hex driver x3: 2 mm across flats x3
•Hex wrench x2: 3 and 4 mm across flats

Chapter 1 Assembly
2
Assembly
1 ECLIPSE Ni-U System Configuration
0.20.40.60.81.01.21.4
Achr-Apl N.A=1.4
EPI FL INTSL
SHUTTEROPEN
12481632
INTSLND
OBJ FL
CONTROL
NI-SRCP
MADEIN JAPAN
DIA
D-FB
Eyelevel riser
Analyzer unit Epi-fluorescence
cube turret*2
Epi-fluorescence
attachment
HG precentered
fiber illuminator
Ergonomic tube
Binocular tube Trinocular tube
DSC port for ergonomic
binocular tube
Centering telescope CFI eyepiece
TV adapter
Ni-U main body
Nosepiece
with
analyzer slot*1
Nosepiece
Analyzer
slider
Stage
CFI objective
Condenser
holder Polarizer
unit
ENG mount camera
C mount camera
Photomicrography device
DS-L3/
DS-U3 Camera
Control Unit
DS camera head
Tube adapter for
quadrocular tube
DSC zooming port
Quadrocular tilting tube
Camera cable to
DS-U3 or DS-L3
Motorized
shutter cable to
control
box B
Motorized shutter
Motorized shutte
r
adapter
Contact arm Standard arm
Lambda plate
DIC slider
DIC
nosepiece*1
To control box B
Filter
cube
FL/DIC
analyzer
Excitation
balancer
RS-232C cable to
control box B for
motorized operation
Motorized
shutter cable to
control
box B
Motorized
shutter
adapter
Motorized
shutte
r
To Ni-U
main body
Control
box B Lamphouse
Simple remote
control pad
PC
Condenser
Substage
Rotatable ceramic
coated sta
g
e
Substage fo
r
rotatable ceramic
coated stage
HG fibe
r
USB cable to
control box B*3
Camera trigger cable to
Ni-U main body or
control box B
*1: A contact arm is required to use a motorized/intelligent nosepiece.
*2: A contact arm is required to use a motorized/intelligent epi-fluorescence cube turret.
*3: DS-U3 DS camera control unit can not be connected via USB.
HG adapter
Various adapters
Mercury lamp

Chapter 1 Assembly
3
Assembly
2 Components List
The ECLIPSE Ni-U components are shown in the table below.
Depending on when you purchased the ECLIPSE Ni-U, there may be devices not yet available for use and devices not
listed that are already available for use. Contact your nearest Nikon representative for details.
Device Device Name Model Remarks
Microscope main
body
ECLIPSE Ni-U (12V 100W 100-240V power supply
for illumination integrated) ECLIPSE Ni-U
Precentered lamphouse (12V 100W) NI-LH
Illuminator for
dia-illumination Halogen lamp (100W) PHILIPS 7724
OSRAM HLX 64623
Standard arm NI-SAM Combine with a manual model such as manual nosepiece
Arm Contact arm NIU-CAM Combine with a motorized models such as intelligent/motorized nosepiece
Binocular tube C-TB C-TBM mildew-proof type also available
Trinocular eyepiece tube F C-TF C-TFM mildew-proof type also available
Trinocular eyepiece tube T C-TT
Ergonomic tube C-TE2
Tube
Quadrocular tilting tube NI-TT
DSC port for ergonomic binocular tube C-TEP2
DSC port DSC zooming port for quadrocular tube NI-RPZ
Connected to Nikon DS camera head or other C mount camera. Nikon DS camera
head is controlled from DS-L3/DS-U3.
CFI eyepiece CFI
CFI UW
Eyepiece
Centering telescope C-CT
Universal quintuple nosepiece L-NU5
BD quintuple nosepiece L-NBD5
Sextuple nosepiece C-N
Sextuple nosepiece with analyzer slot C-NA
Sextuple DIC nosepiece D-ND6
Intelligent DIC sextuple nosepiece NI-ND6-I
Intelligent Septuple nosepiece NI-N7-I
Motorized DIC sextuple nosepiece NI-ND6-E
Nosepiece
Motorized septuple nosepiece NI-N7-E
Objective CFI objective
CFI
LU Plan Fluor Epi
LU Plan Fluor BD
LU Plan Fluor Epi/BD can be attached to the L-NU5 or L-NBD5 nosepiece.
LU nosepiece adapter is required to attach a non-BD objective to L-NBD5.
Substage NI-SS Rotatable ceramic coated stage can not be attached
Substage Substage for rotatable ceramic coated stage NI-SSR
Right handle stage with 2S holder C-SR2S
Left handle stage with 2S holder C-SL2S
Right handle ceramic coated stage with 1S holder C-CSR1S
Left handle ceramic coated stage with 1S holder C-CSL1S
NI-U right handle rotatable ceramic coated stage
with holder NIU-CSRR2
Stage
NI-U left handle rotatable ceramic coated stage with
holder NIU-CSLR2
Condenser holder Condenser holder NI-CH
Dark field condenser (Oil) and (Dry)
C-C aplanatic condenser
C-C achromat condenser No.9
C-C abbe condenser No.9
X LWD condenser
C-C slide achro condenser 2-100x
C-C achromat swing-out condenser 1-100x
C-C phase turret condenser Cannot be used when rotatable ceramic coated stage is attached.
DIC condenser (Oil) D-CUO Used with OIL DIC module
Condenser
Universal condenser (Dry) NI-CUD Supports DRY DIC module, PH module, dark field module, NI-CALN1 2-4x auxiliary
lens, D-C 2-4x auxiliary lens
Cube turret NI-FLT6
Intelligent epi-fluorescence cube turret NI-FLT6-I
Epi-fluorescence
cube turret Motorized epi-fluorescence cube turret NI-FLT6-E
A filter cube and NI-FA FL/DIC analyzer attachable.
Epi-fluorescence
attachment Epi-fluorescence attachment NI-FLEI D-FB excitation balancer attachable.
HG precentered fiber illuminator C-HGFI
Mercury lamp
illuminator Motorized HG precentered fiber illuminator C-HGFIE
Analyzer slider for DIC D-DA Can be attached to C-NA, D-ND6, NI-ND6-I, NI-N7-I, NI-ND6-E, or NI-N7-E
nosepiece.
Analyzer tube for simple polarization C-ISA Combined with C-SP polarizer unit.
Analyzer tube for first-order red compensation C-IA Combined with C-TP polarizer unit.
Analyzer slider for simple polarization D-SA
Analyzer slider for first-order red compensation C-AS
DIC slider D-C Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece.
Analyzer unit/
Slider
Lambda plate D-LP Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E nosepiece.
DIC rotatable polarizer unit D-DP
Polarizer unit for simple polarization C-SP
Polarizer unit
Polarizer unit for first-order red compensation C-TP
Motorized shutter NI-SH-E For connection, NI-SHCL motorized shutter cable is required and is connected to the
control box B.
Motorized shutter adapter for upright epi-fl NI-SHAEP-U
Shutter
Motorized shutter adapter for dia-illumination NI-SHADI
Control box B NI-CTLB
Simple remote control pad NI-SRCP
Controller
DS-L3/DS-U3 camera control unit DS-L3
DS-U3
DS-L3 is connected to the control box B via USB cable
DS-L3/DS-U3 is connected to the Ni-U main body or the control box B via C-CTC
camera trigger cable L3/U3
DS camera I/F cable is used to connect to the camera
Eyelevel riser Eyelevel riser C-ER
Variable intermediate
magnification unit Variable intermediate magnification unit Y-IM
DSC support column NI-RPS
DSC retention
support DSC adapter A and B NI-RPSAA
NI-RPSAB
Tube adapter for
quadrocular tube Tube adapter for quadrocular tube C-TAQ Can be attached to the quadrocular tilting tube. Attach a C mount camera, ENG mount camera or a photomicrography device via various adapters.
TV adapter TV adapter Y-T V
Y-T V55
Y-TV can be attached to C-TF or C-TT trinocular tube. Connect a C mount camera,
ENG mount camera, or photomicroscopy device with an adapter.
Y-TV55 can be attached to C-TF or C-TT trinocular tube. Connect a C mount camera
with C mount adapter 0.55x.

Chapter 1 Assembly
4
Assembly
3 Assembly Method
Introduction
Ni-U is a manual microscope. Basically, a manual model is attached for each device consisting the microscope.
However, for nosepiece, epi-fluorescence cube turret, and HG precentered fiber illuminator, motorized models
can be attached. In this case, contact arm, control box B, and simple remote control pad are required. In addition,
in order to configure information for motorized devices, DS-L3 DS camera control unit must be connected.
Motorized devices on Ni-U can be controlled from DS-L3.
Note that when using epi-fluorescence cube turrets by layering, motorized/intelligent epi-fluorescence cube turret
can only be used in 1st layer.
Assemble the device according to the following procedure.
Precautions for connecting cables
Cable connections are required for some motorized devices.
Be sure to turn off the power to the microscope and peripheral devices before starting cable connection.
Cable connections are described in each assembly step. Nikon recommends connecting cables at the end of the
assembly. See “20 Connect the motorized device cable.” for the connector connections.
Information setting regarding motorized models
Set information of the motorized devices or optical element attached on the microscope setup menu for the DS-L3 DS Camera
Control Unit. (See Chapter 3 “19 Operations on DS-L3” - “19.1 Setting Up the Microscope.)
1
Check the input voltage.
Check the input voltage indicated on the back of the
microscope. Use the microscope only if the indicated
input voltage matches the power supply voltage for the
area in which the microscope will be used.
WARNING
If the indicated voltage and the supplied voltage differ, do
not attempt to use the microscope. Contact your nearest
Nikon representative.
MODEL ECLIPSE Ni-U
NIKON CORPORATION
TOKYO, JAPAN
100–240V~
1.7A
50/60Hz MADE IN JAPAN
This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including interference that may cause undesired operation.
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
920001
INSPECTION
EQUIPMENT
4N75
LAMP
DC12V 100W
DSC
LAMP CTRL
(BOX B)
Checking the input voltage
(Microscope rear view)
Unlocking the elevating section
Tool: flathead screwdriver
After you confirm that the input voltage is correct,
loosen and remove the tightening screw at the front
of the elevating section.
POWER
ND
8ND
32 NCB
11
OUT
IN
Unlocking the elevating section
Input voltage
label
Tightening
screw

Chapter 1 Assembly
5
Assembly
2
Attach the DIA motorized shutter (optional).
The motorized shutter is attached by Nikon.
Contact your nearest Nikon representative when the DIA motorized shutter needs to be attached or removed.
3
Attach the lamp.
Tool: Hex wrench (3 mm across flats)
Lamp handling precautions
Avoid touching the glass surface of the lamp with
your bare hands.
(1) Loosen the lamphouse cover fixing screw and lift up
the cover to remove.
(2) Insert the lamp clamp lever to open the socket pin
hole. Attach the lamp while holding down the lever.
Put the lamp clamp lever back to its original position.
Designated lamp: PHILIPS7724
or OSRAM HLX64623
(3) Reattach the cover back to its original position and
tighten the lamphouse cover fixing screw.
Removing the lamphouse cover
Attaching the lamp
4
Attach the lamphouse for dia-illumination.
Tool: Hex driver (2 mm across flats)
(1) Insert the lamphouse at the rear of the microscope
and tighten the fixing screws.
(2) Connect the dia-illumination lamphouse cable to the
LAMP connector on the rear of the main body.
DSC
LAMP CTRL
(BOX B)
LAMP
DC12V 100W
MODEL ECLIPSE Ni-U
NIKON CORPORATION
TOKYO, JAPAN
100–240V~
1.7A
50/60Hz MADE IN JAPAN
Thisdevice complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1)this device may not cause harmful interference, and
(2)this device must accept any interference received, including interference that may cause undesired operation.
ThisClass A digital apparatus complies with Canadian ICES-003.
Cetappareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
920001
INSPECTION
EQUIPMENT
4N75
1.
2.
3.
Do not touch the lamphouse while the lamp is lit.
The surface of the lamphouse becomes hot when the
lamp is on.
Turn off the power and allow the lamp and lamohouse
to cool enough before replacing the lamp.
Wait for at least 30 minutes after turning off the
lamp.
Use 12V100W HALOGEN lamp only.
HALOGEN 12V100W
952001
NI-LH
CAUTION!
-High Temperature-
JAPAN
Securing the dia-illumination lamphouse
Lamphouse
fixing screw
Fixing screw
Lamphouse
cover
Insert the hex wrench and
loosen the fixing screw.
Lamp
clamp lever
Lamp
Lamphouse fixing screw

Chapter 1 Assembly
6
Assembly
5
Attach the standard arm/contact arm.
The attachment procedure is the same for standard arm
and contact arm. To use the motorized accessories,
attach the contact arm. The contact arm has a contact.
When attaching a motorized device on the arm, remove
the contact cover beforehand.
Tool: Hex wrench (4 mm across flats)
(1) Place the arm while aligning it with the two
positioning pins on the main body and tighten the
four fixing screws.
(2) When using contact arm, connect the cable from the
CONTACT ARM1 connector to the CONTACT ARM1
connector on the control box B.
(3) Connect the cable from the CONTACT ARM2
connector to the CONTACT ARM2 connector on the
control box B.
(Standard arm)
(Contact arm)
Attaching the arm
A
rm fixing screw
Positioning pin
A
rm fixing screw
Contact cover

Chapter 1 Assembly
7
Assembly
6
Attach the epi-fluorescence cube turret and epi-fluorescence attachment (required for
epi-fluorescence microscopy).
Tool: Hex driver (2 mm across flats)
Hex wrench (3 mm across flats)
First, connect the epi-fluorescence cube turret and
epi-fluorescence attachment and then attach them on the
microscope arm.
(1) Insert the positioning pin of the epi-fluorescence
attachment while aligning it with the positioning hole
on the epi-fluorescence cube turret side, and then
tighten the connection fixing screws (x2) on the
epi-fluorescence cube turret with a hex driver.
(2) When using a motorized or intelligent
epi-fluorescence cube turret, remove the contact
cover on the contact arm beforehand.
(Contact cover is not removed for manual
epi-fluorescence cube turret.)
(3) Loosen the fixing screw on the front of the arm using
a hex driver so that the tip of the screw does not
protrude into the connecting section.
(4) Align the round dovetail and convex contact on the
bottom of the epi-fluorescence cube turret with the
round dovetail and concave contact of the arm and
slide the entire mounted attachment to the rear.
(There is no concave contact in manual
epi-fluorescence cube turret.)
(5) Tighten the fixing screw loosened in step (3).
(6) Tighten the four fixing screws on top of the
epi-fluorescence attachment.
(7) Attach the HG adapter to the bayonet mount at the
rear of the epi-fluorescence attachment and connect
the HG precentered fiber illuminator. (See the
instruction manual provided with the HG
precentered fiber illuminator.)
F. STO P A. STOP
EX.ADJ
Connecting epi-fluorescence cube turret
and epi-fluorescence attachment
F. ST OP A. STOP
EX. ADJ
2
1
2
3
4
5
6
Attaching and fixing epi-fluorescence cube turret
and epi-fluorescence attachment set
MODEL ECLIPSE Ni-U
NIKON CORPORATION
TOKYO, JAPAN
100–240V~
1.7A
50/60Hz MADE IN JAPAN
Thisdevice complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1)this device may not cause harmful interference, and
(2)this device must accept any interference received, including interference that may cause undesired operation.
ThisClass A digital apparatus complies with Canadian ICES-003.
Cetappareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
920001
INSPECTION
EQUIPMENT
4N75
F. ST OP A. STOP
EX. ADJ
Attaching the HG adapter
Epi-fluorescence
cube turret Positioning hole
Epi-fluorescence
attachment
Connection fixing screw
Epi-fluorescence
attachment
fixing screw
Fixing screw
Bayonet mount
HG adapter
HG fiber

Chapter 1 Assembly
8
Assembly
■Attaching a filter cube
Precautions for attaching and removing the cube
•Be sure to check that the light source is turned off before attaching or removing the cube.
•Be sure to check that the power switch for microscope main body is turned off and then attach the cube by
rotating the cube switchover turret. Also for the motorized epi-fluorescence cube turret, attach it by manually
rotating the inside turret.
(1) Pull out the filter cube replacement cover on the
front of the epi-fluorescence cube turret to remove it.
(2) Attach the filter cube to the slot.
(3) Insert the filter cube nameplate into the slot cover
window.
Insert into the same address window as the address
shown inside the slot.
5
6
1
A
λ
6
1
5
4
3
2
1
ND4
ND8
ND16
A
1
2
3
5
UV-2A
EX 330-380
DM 400
BA 420
(4) Turn the cube switchover turret to attach the filter
cubes in the remaining slots and also insert the
nameplates.
For bright-field microscopy
For bright-field microscopy, be sure to make the
address 1 empty.
For motorized/intelligent epi-fluorescence cube
turret, only address 1 can be set to [OPEN].
(5) Restore the slot cover back to its original position.
A
λ
2
6
Attaching a filter cube
Filter cube
replacement
slot cover
Filter cube
A
ddress
indication
Nameplate
window
Cube
switchover
turret
A
ddress
indication

Chapter 1 Assembly
9
Assembly
■Replacing excitation and barrier filters
The excitation filter, barrier filter, and dichroic mirror can
be removed from the cube for replacement.
Excitation filters are screw-in filters, while barrier filters
are slide-in filters.
Align the projection on the barrier filter with the groove
on the filter cube and turn clockwise by approximately 30
degrees to secure it in place.
Orientation of the filter
• The filter is two-faced. Be aware that the filter does
not face a wrong side. Nikon filters have an arrow
indication printed on the outer frame. Make sure that
the arrow faces the direction of a dichroic mirror when
attaching the filter.
• Contact your nearest Nikon representative when the
dichroic mirror needs to be replaced.
UV-2A
EX 330-380
DM 400
BA 420
Replacing the excitation and barrier filters
■Attaching a light shielding plate
Tool: Hex driver (2 mm across flats)
Lightly screw the two fixing screws provided with the light
shielding plate into the front bottom of the
epi-fluorescence cube turret. Hook the convex part of the
light shielding plate to the two fixing screws and tighten
the fixing screws.
2
1
2
3
4
5
6
Fixing the light shielding plate
Fixing screw
(At the bottom of the
epi-fluorescence cube
turret)
Excitation
filter
Barrier filter
Turn approx. 30 degrees
to secure in place.

Chapter 1 Assembly
10
Assembly
7
Attach the quadrocular tilting tube and DSC zooming port for quadrocular tube.
Tools: Hex driver (2 mm across flats)
Hex driver (2.5 mm across flats, provided with DSC zooming port)
Hex wrench (3 mm across flats)
Hex wrench (4 mm across flats)
First connect the quadrocular tilting tube and DSC
zooming port for quadrocular tube, then attach them on
the microscope arm (if epi-fluorescence cube turret or
epi-fluorescence attachment is attached, on top of that).
(1) Remove the tightening screws at the bottom of the
tube with the hex wrench (4 mm across flats).
(2) Insert the positioning pin of the DSC zooming port
while aligning it with the positioning hole on the tube
side, and then tighten the connection fixing screws
(2.5 mm across flats) on the top of the tube with a
hex wrench.
(3) Loosen the fixing screw on the front of the arm using
a hex driver (2 mm across flats) so that the tip of the
screw does not protrude into the connecting section.
(The fixing screw at the front of the turret, if
epi-fluorescence cube turret is attached.)
(4) Loosen the fixing screws (x2) on both sides of the
zooming port with a hex driver (2 mm across flats).
(5) Align the round dovetail of the quadrocular tube with
the round dovetail of the arm and slide the entire
attached devices to the rear.
(6) Tighten the fixing screw loosened in step (3).
(7) Tighten the fixing screws (x2) on top of the zooming
port with a hex wrench (3 mm across flats).
(8) Tighten the fixing screws (x2) loosened in step (4).
(9) Affix the provided sticker to cover the hole for the
zooming port fixing screw.
When attaching on the epi-fluorescence cube turret, the
location of the fixing screws at the front is deep so it will
be easier that you insert the hex driver (2 mm across
flats) into the screw before placing the quadrocular tube
or that you have a pen light when working.
Removing the tube tightening screw
(Tube bottom view)
Connecting the quadrocular tube
and DSC zooming port
0.6
2
1.5
50
60
70
Fixing the quadrocular tube
and DSC zooming port set
8
Attach the EPI motorized shutter (optional).
The motorized shutter is attached by Nikon.
Contact your nearest Nikon representative when the EPI
motorized shutter needs to be attached or removed.
Tightening screw
Connection
fixing screw
Positioning hole
Set screw
Zooming port
fixing screw
Fixing scre
w

Chapter 1 Assembly
11
Assembly
9
Attach eyepieces.
Make sure the notch on the eyepiece side and the
protrusion of the eyepiece sleeve are aligned, then insert
the eyepieces.
Notch on eyepiece
The eyepiece has a notch to prevent rotation. When
attaching, match the notch with the protrusion on the
eyepiece sleeve. The eyepiece lens will not be
positioned properly if the notch is not matched with
the protrusion.
50
60
70
Attaching eyepieces
10
Attach the substage.
Attach NI-SS substage or NI-SSR substage for rotatable
stage suitable for the type of the stage used. The
procedure is the same for both models.
Tools: Hex driver (2 mm across flats)
Hex wrench (3 mm across flats)
(1) Place the square groove on the substage onto the
reference guide bar on the elevating section of the
main body. Move the substage to the left, press it
against the reference surface of the elevating
section, and tighten the drop-proof fixing screws (x3)
with hex wrench (3 mm across flats).
(2) Put the provided elevating section cover onto a front
of the elevating section and tighten the provided two
screws with hex driver (2 mm across flats) to secure.
Attaching the substage
Attaching the elevating section cover
Protrusion
Notch
Substage
fixing screw Reference
guide bar
Square groove
Substage fixing screw
(Substage from the front)
Elevating
section cover

Chapter 1 Assembly
12
Assembly
11
Attach the condenser holder.
Attach a condenser holder to the substage. (Remove or
attach the condenser holder after detaching the stage.)
Tool: Hex wrench (3 mm across flats)
Hook the half dovetail of the condenser holder onto the
half dovetail groove of the lower substage and push it
against the reference surface. Then, tighten the fixing
screw on the top of the substage.
Attaching the condenser holder
12
Attach the stage.
12.1
For standard stage
(1) Turn the coarse focus knob until the substage is
brought to the lowermost position.
(2) Loosen the stage clamp screw on the front of the
stage.
(3) Align the stage with the round dovetail of the
substage and secure it in place with the stage clamp
screw.
POWER
ND
8N
3
OUT
IN
Securing the standard stage
12.2
For rotatable ceramic coated stage
Tools: Hex driver (2 mm across flats)
Centering tool: Ball-point hex drivers provided with
the product (x2)
(1) Sufficiently loosen the two right and left centering
screws on the back of the substage and the stage
positioning screw on the front of the stage using a
hex driver.
(2) Attach by engaging the round dovetail at the bottom
of the stage with the round dovetail of the substage.
(3) Center the stage and then tighten the stage
positioning screw.
(See Chapter 3, “3 Bringing the Target into the
Optical Path (Horizontal Stage Movement, Rotation)
-■Centering the rotatable ceramic coated stage” in
the “Operation”)
When removing the stage
When removing the stage, loosen the stage centering
screws (x2) and stage positioning screw.
POWER
0
90
80
70
60
50
40
30
20
10
Securing the rotatable ceramic coated stage
Stage
clamp screw
Condenser holder
fixing screw
Half dovetail
groove
Half dovetail
Stage positioning
screw
Stage centering screws
(on both sides)