Fisher Scientific 11350119 User manual

Inverted Microscope
Instruction Manual
Model 11350119

2
Introduction
Thank you for your purchase of a Fisher Scientic microscope. Your new microscope is a precision
instrument carefully checked to assure that it reaches you in good condition. It is designed for ease of
operation and years of carefree use. The information in this manual probably far exceeds what you will
need to know in order to operate, troubleshoot and maintain your microscope. However, it is provided
to answer questions that might arise, and to help you avoid any maintenance expense that may be
unnecessary.

3
Fisher Scientic Inverted Microscope
Table of Contents
Nomenclature 4-5
Specications 6
Setting Up the Microscope 7
Assembling the Microscope Input Voltage 7
Installing the Lamp 7
Mounting the Condenser 8
Installing the Objectives 8
Mechanical Stage 8
Mounting the Eyepieces 8
Microscopic Procedure: Interpupillary Distance Adjustment 9
Diopter Adjustment 9
Centering the Condenser 9
Centering the Lamp 10
Brighteld Microscopy 10
Phase Contrast Microscopy 11
Photomicrographic Procedure 12
Troubleshooting Table 13
Care and Maintenance 14
Lenses and Filters 14
Cleaning of Painted or Plastic Components 14
When Not in Use 14
Fisher Scientic Warranty 15

4
Nomenclature
1. Lamp Socket Clamp Screw Knob
2. Lamp House Cover Clamp Screw
3. Annular Diaphragm
4. Condenser Diaphragm Lever
5. Condenser Focus Knob
6. Condenser Lens
7. Stage Plate Insert
8. Objectives
9. Revolving Nosepiece
10. Coaxial Coarse/Fine Focus Knob
11. Torque Adjusting Ring
12. Filter Retaining Ring
13. Hexagonal Centering Screwdrivers (x2)
1
2
3
4
5
6
7
9
8
10
11
12
13

5
1. Vertical Photo Port
2. Interpupillary Distance Scale
3. Diopter Adjustment Ring
4. Eyepiece
5. Optical Path Selector Lever
6. Filter Slider
7. Lamp House
8. Field Diaphragm Lever
9. Condenser Clamp Screw
10. Phase Slider
11. Condenser Clamp Holder Screw
12. Condenser Centering Screws
13. Stage Plate
14. Fluorescence Filter cassette Mount
15. Power Switch
16. Light Intensity Control Dial
17. Brightness Indicator (LED Segmented Display)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17

6
Specications
• Magnication Ratio: 40X - 200X
• Eyepiece: Objective Field Ø22
• Objectives:
• Condenser: N.A. 0.3 / W.D. 72mm
• Electrical Specications:
»Input: 90-240V~, 35W, 50-60Hz
»Lamp: DC6V, 30W Halogen
»Fuse: 250V T2.5A
(If the original fuse is damaged, please replace with a new one.)
Magnication N.A. W.D. (mm)
Plan Achromat 4X 0.10 23.5
Plan Achromat Phase 10X 0.25 7.5
Plan Achromat Phase LWD 20X 0.40 7.0

7
Setting Up the Microscope
Working environment
• The location should be free from dust, moisture, chemical vapors and mechanical vibrations.
• Do not place the instrument in a warm and/or humid environment.
• Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a well-
lit bright wall. The quality of the viewed image from the microscope will deteriorate where there is signicant
ambient light.
Assembling the microscope Input voltage
• Automatic voltage selection works with electrical outlets worldwide. However, always use a power cord that
is rated for the voltage used in your area and that has been approved to meet local safety standards. Using
the wrong power cord could cause re or equipment damage.
• In case of using the extension cord, use only the power supply cord with the PE (protective earth) wire.
• In order to prevent electric shock, always turn the power switch on the power supply off before connecting
the power cord.
1. Installing the lamp
• In order to prevent electric shock always turn the power switch off and unplug the power cord before
replacing the lamp.
• Release lamp housing cover clamp screw using a coin to remove the cover.
• Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the
• lamp when mounting.
• When installing the lamp, do not touch the glass surface of the lamp with bare ngers. Doing so
• will cause ngerprints, grease, etc., to burn onto the lamp surface, reducing the illumination provided
by the lamp. If surface is contaminated, wipe it clean using lens tissue.
• Close the cover and fasten with it with lamp housing cover clamp screw.
Power Cord
Power Switch
Lamp Housing
Cover Clamp
Screw

8
• Insert the lter slider with mat surface of the diffuser turned towards the user.
2. Mounting the Condenser
• Mount the ELWD condenser on the circular dovetail mount of the condenser holder with the aperture
diaphragm lever facing toward the eyepieces.
• Insert the Ph annular diaphragm slider with centering hexagonal socket head screws facing
• the front.
• The centerable condenser mount is height adjustable with rack and pinion and is dovetail
• mounted on the illuminating pillar with a clamp screw.
3. Installing the Objectives
• Remove the stage plate insert from the stage.
• Install the objectives into the nosepiece so that the magnication increases with clockwise rotation of
the revolving nosepiece.
• Replace the stage plate insert.
4. Mechanical Stage
• Secure the mechanical stage to the 11350119 plain stage using the two mounting screws located
beneath the stage on the right side
5. Mounting the Eyepieces
• Remove the dust caps from the eyepiece tubes.
• Insert the eyepieces into the eyepiece tubes.
• If the rubber eye guards are to be used, t them in the groove around the eyepiece.
Condenser
Clamp Screw

9
Microscopic Procedure: Interpupillary Distance Adjustment
• Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
• Adjust the interpupillary distance so that both the right and left eld of view become one.
• This adjustment will enable the user to observe the specimen with both eyes.
1. Diopter Adjustment
• Diopter adjustment compensates for differences in vision between the left and right eyes. In addition to
making observation through both eyes easier, this adjustment also reduces the extent to which focusing
is lost when the objective magnication is changed. In particular, this occurs when a low power
objective is used.
• Before adjusting the diopter, bring a specimen into focus using the 10x objective.
• Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to “0”
position.
• Position the highest magnication objective into the optical path and bring the specimen image into
focus by turning the coarse and ne focus knobs.
• Position either 4x or 10x objective into optical path. Without adjusting the ne and coarse focus knobs,
turn the diopter rings on the eyepieces so that the specimen images in the left and right eyepieces are
focused individually.
• Repeat the above step twice.
2. Centering the Condenser
• Set the Phase annular diaphragm slider in center position (O).
• Fully open the eld of view diaphragm.
• Move the aperture diaphragm lever in open “O” position.
• Bring the specimen image into focus, using the 10x objective.
• Close the eld of view diaphragm to its minimum setting.
• Turn the condenser focus knob so that the image of the eld diaphragm forms on the specimen
surface.
• Adjust the condenser centering screws so that the center of the eld diaphragm image matches the
center of the eld of view. This adjustment is easier to make if the eld diaphragm size is reduced to
Diopter adjustment
“0” position

10
slightly smaller than the eyepiece eld of view.
• For normal observation, the size of the diaphragm should be just outside the edge of the eld of view.
3. Centering the Lamp
• Set the Phase annular diaphragm slider in the center position (O).
• Fully open the eld of view diaphragm.
• Move the aperture diaphragm lever to the open “O” position.
• Using the 10x Phase contrast objective, bring the specimen image into focus.
• Remove the diffuser lter slider from the light path.
• Remove an eyepiece and insert the phase centering telescope in its place.
• Holding the knurled part of the centering telescope, rotate its eyepiece to focus on the phase plate
image of the objective.
• Release the lamp socket clamp screw using the knob.
• Move the lamp socket with the knurled knob to bring the lamp lament image to the center of the
phase plate image of the objective.
• After nishing the above lamp centering procedure, insert the lter slider with mat surface of the
diffuser turned towards the user.
4. Brighteld Microscopy
• Set the Phase annular diaphragm slider in the center position (O).
• Bring the specimen image into focus.
• Adjust the opening of the eld of view diaphragm, for normal observation the size of the diaphragm
should be just outside the edge of the eld of view.
• The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the
illuminating system of the microscope. It is important because it determines the resolution of the image,
contrast, depth of focus and brightness.
• Reducing the aperture diaphragm will lower the resolution and brightness but increase the contrast and
depth of focus.
Lamp Socket Clamp
Screw Knob