Fisher Scientific 11350119 User manual

Inverted Microscope
Instruction Manual
Model 11350119

2
Introduction
Thank you for your purchase of a Fisher Scientic microscope. Your new microscope is a precision
instrument carefully checked to assure that it reaches you in good condition. It is designed for ease of
operation and years of carefree use. The information in this manual probably far exceeds what you will
need to know in order to operate, troubleshoot and maintain your microscope. However, it is provided
to answer questions that might arise, and to help you avoid any maintenance expense that may be
unnecessary.

3
Fisher Scientic Inverted Microscope
Table of Contents
Nomenclature 4-5
Specications 6
Setting Up the Microscope 7
Assembling the Microscope Input Voltage 7
Installing the Lamp 7
Mounting the Condenser 8
Installing the Objectives 8
Mechanical Stage 8
Mounting the Eyepieces 8
Microscopic Procedure: Interpupillary Distance Adjustment 9
Diopter Adjustment 9
Centering the Condenser 9
Centering the Lamp 10
Brighteld Microscopy 10
Phase Contrast Microscopy 11
Photomicrographic Procedure 12
Troubleshooting Table 13
Care and Maintenance 14
Lenses and Filters 14
Cleaning of Painted or Plastic Components 14
When Not in Use 14
Fisher Scientic Warranty 15

4
Nomenclature
1. Lamp Socket Clamp Screw Knob
2. Lamp House Cover Clamp Screw
3. Annular Diaphragm
4. Condenser Diaphragm Lever
5. Condenser Focus Knob
6. Condenser Lens
7. Stage Plate Insert
8. Objectives
9. Revolving Nosepiece
10. Coaxial Coarse/Fine Focus Knob
11. Torque Adjusting Ring
12. Filter Retaining Ring
13. Hexagonal Centering Screwdrivers (x2)
1
2
3
4
5
6
7
9
8
10
11
12
13

5
1. Vertical Photo Port
2. Interpupillary Distance Scale
3. Diopter Adjustment Ring
4. Eyepiece
5. Optical Path Selector Lever
6. Filter Slider
7. Lamp House
8. Field Diaphragm Lever
9. Condenser Clamp Screw
10. Phase Slider
11. Condenser Clamp Holder Screw
12. Condenser Centering Screws
13. Stage Plate
14. Fluorescence Filter cassette Mount
15. Power Switch
16. Light Intensity Control Dial
17. Brightness Indicator (LED Segmented Display)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17

6
Specications
• Magnication Ratio: 40X - 200X
• Eyepiece: Objective Field Ø22
• Objectives:
• Condenser: N.A. 0.3 / W.D. 72mm
• Electrical Specications:
»Input: 90-240V~, 35W, 50-60Hz
»Lamp: DC6V, 30W Halogen
»Fuse: 250V T2.5A
(If the original fuse is damaged, please replace with a new one.)
Magnication N.A. W.D. (mm)
Plan Achromat 4X 0.10 23.5
Plan Achromat Phase 10X 0.25 7.5
Plan Achromat Phase LWD 20X 0.40 7.0

7
Setting Up the Microscope
Working environment
• The location should be free from dust, moisture, chemical vapors and mechanical vibrations.
• Do not place the instrument in a warm and/or humid environment.
• Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a well-
lit bright wall. The quality of the viewed image from the microscope will deteriorate where there is signicant
ambient light.
Assembling the microscope Input voltage
• Automatic voltage selection works with electrical outlets worldwide. However, always use a power cord that
is rated for the voltage used in your area and that has been approved to meet local safety standards. Using
the wrong power cord could cause re or equipment damage.
• In case of using the extension cord, use only the power supply cord with the PE (protective earth) wire.
• In order to prevent electric shock, always turn the power switch on the power supply off before connecting
the power cord.
1. Installing the lamp
• In order to prevent electric shock always turn the power switch off and unplug the power cord before
replacing the lamp.
• Release lamp housing cover clamp screw using a coin to remove the cover.
• Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the
• lamp when mounting.
• When installing the lamp, do not touch the glass surface of the lamp with bare ngers. Doing so
• will cause ngerprints, grease, etc., to burn onto the lamp surface, reducing the illumination provided
by the lamp. If surface is contaminated, wipe it clean using lens tissue.
• Close the cover and fasten with it with lamp housing cover clamp screw.
Power Cord
Power Switch
Lamp Housing
Cover Clamp
Screw

8
• Insert the lter slider with mat surface of the diffuser turned towards the user.
2. Mounting the Condenser
• Mount the ELWD condenser on the circular dovetail mount of the condenser holder with the aperture
diaphragm lever facing toward the eyepieces.
• Insert the Ph annular diaphragm slider with centering hexagonal socket head screws facing
• the front.
• The centerable condenser mount is height adjustable with rack and pinion and is dovetail
• mounted on the illuminating pillar with a clamp screw.
3. Installing the Objectives
• Remove the stage plate insert from the stage.
• Install the objectives into the nosepiece so that the magnication increases with clockwise rotation of
the revolving nosepiece.
• Replace the stage plate insert.
4. Mechanical Stage
• Secure the mechanical stage to the 11350119 plain stage using the two mounting screws located
beneath the stage on the right side
5. Mounting the Eyepieces
• Remove the dust caps from the eyepiece tubes.
• Insert the eyepieces into the eyepiece tubes.
• If the rubber eye guards are to be used, t them in the groove around the eyepiece.
Condenser
Clamp Screw

9
Microscopic Procedure: Interpupillary Distance Adjustment
• Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
• Adjust the interpupillary distance so that both the right and left eld of view become one.
• This adjustment will enable the user to observe the specimen with both eyes.
1. Diopter Adjustment
• Diopter adjustment compensates for differences in vision between the left and right eyes. In addition to
making observation through both eyes easier, this adjustment also reduces the extent to which focusing
is lost when the objective magnication is changed. In particular, this occurs when a low power
objective is used.
• Before adjusting the diopter, bring a specimen into focus using the 10x objective.
• Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to “0”
position.
• Position the highest magnication objective into the optical path and bring the specimen image into
focus by turning the coarse and ne focus knobs.
• Position either 4x or 10x objective into optical path. Without adjusting the ne and coarse focus knobs,
turn the diopter rings on the eyepieces so that the specimen images in the left and right eyepieces are
focused individually.
• Repeat the above step twice.
2. Centering the Condenser
• Set the Phase annular diaphragm slider in center position (O).
• Fully open the eld of view diaphragm.
• Move the aperture diaphragm lever in open “O” position.
• Bring the specimen image into focus, using the 10x objective.
• Close the eld of view diaphragm to its minimum setting.
• Turn the condenser focus knob so that the image of the eld diaphragm forms on the specimen
surface.
• Adjust the condenser centering screws so that the center of the eld diaphragm image matches the
center of the eld of view. This adjustment is easier to make if the eld diaphragm size is reduced to
Diopter adjustment
“0” position

10
slightly smaller than the eyepiece eld of view.
• For normal observation, the size of the diaphragm should be just outside the edge of the eld of view.
3. Centering the Lamp
• Set the Phase annular diaphragm slider in the center position (O).
• Fully open the eld of view diaphragm.
• Move the aperture diaphragm lever to the open “O” position.
• Using the 10x Phase contrast objective, bring the specimen image into focus.
• Remove the diffuser lter slider from the light path.
• Remove an eyepiece and insert the phase centering telescope in its place.
• Holding the knurled part of the centering telescope, rotate its eyepiece to focus on the phase plate
image of the objective.
• Release the lamp socket clamp screw using the knob.
• Move the lamp socket with the knurled knob to bring the lamp lament image to the center of the
phase plate image of the objective.
• After nishing the above lamp centering procedure, insert the lter slider with mat surface of the
diffuser turned towards the user.
4. Brighteld Microscopy
• Set the Phase annular diaphragm slider in the center position (O).
• Bring the specimen image into focus.
• Adjust the opening of the eld of view diaphragm, for normal observation the size of the diaphragm
should be just outside the edge of the eld of view.
• The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the
illuminating system of the microscope. It is important because it determines the resolution of the image,
contrast, depth of focus and brightness.
• Reducing the aperture diaphragm will lower the resolution and brightness but increase the contrast and
depth of focus.
Lamp Socket Clamp
Screw Knob

11
5. Phase Contrast Microscopy
• Phase contrast objectives are labeled Ph.
• Fully open the aperture diaphragm.
• Bring the 10x (Ph1) objective into optical path.
• Position the Phase annular diaphragm slider to 10 -20. Set slider to 40 when using a 40x (Ph3)
objective (optional).
• Remove either eyepiece from the eyepiece tube and insert the phase centering telescope in its place.
• Rotate the eyepiece of the centering telescope to focus on both the phase plate image of the objective
and the annular diaphragm image of the phase slider.
• If the objective phase plate and the annular of the slider do not coincide, use the two hexagonal
screwdrivers supplied with the microscope to bring the slider annular ring to the center of the phase
plate, so that the image of the annular diaphragm is concentric with the phase plate image.
• If the slider annular ring image is moved from the phase plate image in the objective, a low phase
contrast image will result.
• For phase contrast microscopy at the maximum contrast, use GIF (Green Interference Filter) in the
optical path.
• Place the lter in the designated retaining ring above the phase annular diaphragm slider.

12
Photomicrographic Procedure
• The optical path selector lever can be used to set the optical path to either the binocular tube 100:0/0:100
(beam split for uorescence) or binocular tube/vertical tube 20:80/100:0 (for brighteld or phase).
• Before starting photomicrography, check the following:
• The condenser is centered.
• The condenser annular diaphragm is centered.
• The eld of view diaphragm is reduced to slightly just outside the edge of the eld of view.
• For photomicrographic procedures, refer to the manual of the specic camera being used.
Filter Selection
Filter Type Procedure
GIF (Green Interference) 546nm For phase contrast and contrast adjustment with black and white lm.
NCB (Neutral Color Balance) Blue For general microscopy and color photos.
Never attempt either of the following actions. Doing so will damage the focusing mechanism.
• Rotate the left and right knob while holding the other.
• Turning the coarse and ne focus knobs further than their limit.

13
Troubleshooting Table
The troubleshooting table below contains the most frequently encountered problems and their possible causes.
Optical and Operating Problems
Electrical
Problem Possible Cause
Vignetting or uneven brightness
in the eld of view or eld of view
only partially visible
• Lamp not installed properly
• Filter slider in intermediate position
• Phase slide not in click-stop position
• Incorrect condenser mounting
• Condenser is set too low
• Condenser is not centered
• Field diaphragm is closed too far
• Aperture diaphragm is closed too far
• Revolving nosepiece not clicked into position
• Optical path selector in intermediate position
Dust or dirt in eld of view
• Aperture diaphragm is closed too far
• Field of view diaphragm not focused on specimen surface
• Dust or dirt on specimen’s surface
Image quality: No image under
phase contrast or details cannot
be viewed
• Brighteld objective being used
• Phase annular diaphragm not in optical path
• Phase annular diaphragm and objective phase symbol do not match
• Slider annual ring image has moved away from the objective phase
plate image
• Field of view diaphragm image not focused on specimen surface
• Thickness of specimen holder is outside the compensating range of
objective
Eye strain or fatigue
• Interpupillary distance not adjusted
• Diopter adjustment not made
• Inadequate illumination
• Field of view of left and right eyepiece differ
Problem Possible Cause
Lamp does not light
• Power supply not plugged in
• Lamp not installed
• Lamp burnt out
Inadequate brightness • Specied lamp not being used
Lamp blows out immediately • Specied lamp not being used
Lamp ickers
• Connectors are not securely connected
• Lamp near end of service life
• Lamp not securely plugged into socket

14
Care and Maintenance
1. Lenses and Filters
• To clean lens surfaces or lters, rst remove dust using compressed air. If dust still persists, use a soft/
clean brush or gauze.
• If excessive dirt or grease gets on lens surfaces, a small amount of distilled water or eyeglass lens
cleaner can be used on a lens tissue.
• Do not use same area of gauze or tissue, to wipe more than once.
2. Cleaning of Painted or Plastic Components
• Do not use organic solvents (thinners, alcohol, ether, etc.). Doing so could result in discoloration or in
the peeling of paint.
• For stubborn dirt, moisten a piece of gauze with diluted detergent and wipe clean.
3. When Not in Use
• When not in use, cover the instrument with dust cover and store in a place low in humidity where mold
is not likely to form.
• Store the objectives, eyepieces and lters in a container or desiccator with drying agent.

15
Fisher Scientic Warranty
The Fisher Scientic 5 Year Warranty assures that the microscope is guaranteed against defects in material and
workmanship for 5 years from the purchase date of the product. Electrical components are covered for three
years; video components are covered for one year after purchase. Normal wear, routine maintenance, light
bulbs, power supplies, rechargers, batteries, fuses, cords, add-on accessories, damage resulting from repair by
unauthorized parties, accident, alteration, shipping, misuse or abuse is not covered. Warranty service is provided
by National Optical & Scientic Instruments, Inc.’s authorized technicians. Determination of warranty is at the
technician’s discretion.
The Fisher Scientic Warranty for cameras is 1 year.
Other than set forth above, Fisher Scientic hereby disclaims all warranties, expressed or implied, of tness for a
particular purpose.
Defective products covered by the warranty will be repaired free of charge when they are returned, postpaid, to:
Fisher Scientic
c/o National Optical & Scientic Instruments, Inc.
Attn: Warranty Repair
6508 Tri-County Parkway
Schertz, TX 78154
For all warranty repairs or service requests, please call Fisher Scientic repair department at [phone]
before anything is shipped. This warranty gives you specic legal rights, and you may also have other rights
which vary from state to state.
*For customers living outside the United States, Fisher Scientic will provide standard warranty service.
However, inbound and outbound shipping cost is the responsibility of the consumer.

© 2014 Thermo Fisher Scientific Inc. All rights reserved.