Thermo Scientific Nicolet iN10 Guide

Using Your Nicolet iN10 FT-IR Microscope

The information in this publication is provided for reference only. All
information contained in this publication is believed to be correct and
complete. Thermo Fisher Scientific shall not be liable for errors
contained herein nor for incidental or consequential damages in
connection with the furnishing, performance or use of this material. All
product specifications, as well as the information contained in this
publication, are subject to change without notice.
This publication may contain or reference information and products
protected by copyrights or patents and does not convey any license
under our patent rights, nor the rights of others. We do not assume
any liability arising out of any infringements of patents or other rights
of third parties.
We make no warranty of any kind with regard to this material,
including but not limited to the implied warranties of merchantability
and fitness for a particular purpose. Customers are ultimately
responsible for validation of their systems.
© 2008 Thermo Fisher Scientific Inc. All rights reserved. No part of
this publication may be stored in a retrieval system, transmitted, or
reproduced in any way, including but not limited to photocopy,
photograph, magnetic or other record, without our prior written
permission.
For Technical Support, please contact:
Thermo Fisher Scientific
5225 Verona Road
Madison, WI 53711-4495 U.S.A.
Telephone: [phone]
E-mail: [email protected]
World Wide Web: http://www.thermo.com/spectroscopy
For International Support, please contact:
Thermo Fisher Scientific
Telephone: [phone]
E-mail: [email protected]
World Wide Web: http://www.thermo.com/spectroscopy
Windows is either a trademark or a registered trademark of Microsoft
Corporation in the United States and/or other countries. All other
trademarks are the property of Thermo Fisher Scientific Inc. and its
subsidiaries.

Table of Contents
Introduction ...............................................................................................................3
Welcome .................................................................................................................3
Questions and concerns.............................................................................................3
Conventions used in this help system ..........................................................................3
Warranty information ................................................................................................4
Trademarks .............................................................................................................4
Purge/desiccation requirement ...................................................................................5
Finding PDF documents .............................................................................................5
Ordering parts..........................................................................................................5
Safety .......................................................................................................................6
Safety precautions....................................................................................................6
WEEE compliance .....................................................................................................6
Safety labels............................................................................................................7
Safety and electrical symbols .....................................................................................8
Dangerous contaminants ...........................................................................................9
Using liquid nitrogen .................................................................................................9
Using corrosives and solvents.....................................................................................9
Checking the power supply ...................................................................................... 10
Operation................................................................................................................. 11
Turning the power on and off ................................................................................... 11
Aligning your instrument.......................................................................................... 11
Positioning a manual stage....................................................................................... 11
Positioning a motorized stage................................................................................... 12
Setting the purge gas controls.................................................................................. 12
Cooling a detector .................................................................................................. 14
Installing a slide-on ATR attachment ......................................................................... 15
Using the purge collar ring....................................................................................... 16
Collecting data with the instrument ........................................................................... 16
Service .................................................................................................................... 17
Static electricity precautions..................................................................................... 17
Installing a purge kit............................................................................................... 17
Replacing the power supply...................................................................................... 20
Cleaning your instrument......................................................................................... 21
Maintaining detector dewars..................................................................................... 21
Checking the humidity indicator................................................................................ 22
Replacing the humidity indicator ............................................................................... 23
Replacing the desiccant ........................................................................................... 24
Regenerating the desiccant ...................................................................................... 26
Checking and changing the purge gas filter ................................................................ 27
Index ...................................................................................................................... 31

This page is intentionally left blank.

3
Introduction
Welcome
The Thermo Scientific Nicolet™ iN™10 microscope has integrated validation features, a
powerful software suite, and many other features that make it easy for you to collect data. It
was designed to allow you to perform several service and maintenance procedures yourself,
and this help system, or printed documentation, contains detailed information about
performing such procedures. If you cannot find the information you are looking for, please
contact us for assistance.
If you are using the electronic help version of this document, you can access information by
double-clicking the book in the Contents tab that interests you, and then double-clicking the
topic you want to read. There is also an Index tab where you can search for topics by phrase
or keyword, and there is a Find tab that allows you to search for specific words.
Note: If you have a Nicolet iZ™10 Module connected to your microscope, please refer to the
Nicolet iZ10 help system for information about using the module.
Questions and concerns
In case of emergency, follow the procedures established by your facility. If you have questions
or concerns about safety or need assistance with operation, repairs or replacement parts, you
can contact our sales or service representative in your area or visit our web site at
www.thermo.com/spectroscopy.
Conventions used in this help system
This help system includes safety precautions and other important information presented in the
following format:
Note: Notes contain helpful supplementary information.
Notice: Follow instructions labeled "Notice" to avoid damaging the system hardware or losing
data.
Caution: Indicates a hazardous situation which, if not avoided, could result in minor or
moderate injury.
Warning: Indicates a hazardous situation which, if not avoided, could result in death or
serious injury.
Danger: Indicates a hazardous situation which, if not avoided, will result in death or
serious injury.

Warranty information
Thermo Fisher Scientific warrants that each product we sell you is free from defects in labor
and materials and shall conform to its product specifications as defined in the product user
documentation. If the product does not function as warranted during the warranty period, we
will repair or replace it without charge. If in our judgment we are unable to do so, you may
return it to us and we will refund your money.
This warranty replaces all other warranties, expressed or implied, including the implied
warranties of merchantability and fitness for a particular purpose and any other obligations or
liabilities on the part of Thermo Fisher Scientific whether in contract, warranty, negligence or
otherwise. Thermo Fisher Scientific shall not be liable for and disclaims all consequential,
incidental and contingent damages.
Warranty period
The system warranty period is 12 months in the U.S.A. and Canada. The warranty period
begins on the date of installation or 30 days from the date of invoice, whichever is sooner.
The system warranty period for products sold outside the U.S.A. and Canada is 12 months
from the date of installation or 14 months from the date of shipment, whichever is sooner.
Limit of warranty
Misuse, accident, modification, unsuitable physical or operating environment, improper
maintenance, or damage caused by a product for which we are not responsible will void the
warranty. Certain components may have separate warranty periods as stated in the product
user documentation. Consumables are not covered under warranty.
Items not covered by warranty
We do not warrant uninterrupted or error-free operation of a product. We provide certain non-
Thermo Fisher Scientific products on an "as is" basis. Non-Thermo Fisher Scientific
manufacturers or suppliers may provide their own warranties to you. A separate software
warranty is provided with the software user documentation.
Trademarks
Windows is either a trademark or registered trademark of Microsoft Corporation in the United
States and/or other countries. All other trademarks are the property of Thermo Fisher
Scientific Inc. and its subsidiaries.
4

Purge/desiccation requirement
We recommend that you maintain seal and desiccation and/or purge your instrument at all
times. Equipment damage due to failure to maintain seal and desiccation and/or purge is not
covered under the warranty. If you have questions about this requirement, please contact us.
Finding PDF documents
PDF files of your instrument documentation are installed on the hard drive of the system
computer when the software is installed. To access these PDFs:
1.—Click the Start button on the Windows®taskbar.
2.—Click All Programs.
3.—Click the Thermo Scientific OMNIC™ folder.
4.—Click the Documentation folder.
5.—Choose the PDF file you want to open.
Ordering parts
To order parts, contact us.
5

Safety
Safety precautions
The United States Department of Health and Human Services warns against improper laser
use, as follows:
Warning: Use of controls or adjustments or performance of procedures other than those
specified in your printed and electronic documentation may result in hazardous radiation
exposure. Do not alter or attempt to remove the laser head from its protective housing or
attempt to remove the shields that surround the laser head. Exposure to laser energy and
high voltage may result.
Warning: Never stare directly into the laser beam or at its bright reflection. Avoid skin
contact with the laser beam or its bright reflection. Never tamper with the laser head.
Exposure to laser light or high voltage may result.
Warning: When operating your instrument, use only equipment (such as power supplies
and lasers) supplied by us. Use of equipment (such as power supplies and lasers) not supplied
by us can result in permanent damage to the instrument and may create a safety hazard.
Caution: The instrument may contain an automated stage that is controlled by the
software. There are several places on and around the stage where a person or object could be
pinched by moving parts. When operating the system, keep hands and fingers away from the
stage area except to position the sample or change accessories. Remove your hands from the
system when operating the software and when the stage is moving.
WEEE compliance
Many instruments are required to comply with the European Union’s Waste Electrical &
Electronic Equipment (WEEE) Directive 2002/96/EC. If compliance is required, the instrument
is marked with the following symbol:
We have contracted with one or more recycling/disposal companies in each EU Member State,
and this product should be disposed of or recycled through them. Further information on our
compliance with these directives, the recyclers in your country, and information on our
products which may assist the detection of substances subject to the RoHS Directive are
available at www.thermo.com/WEEERoHS.
6

Safety labels
The safety labels on your instrument are shown below. To order new labels, contact us.
7

Safety and electrical symbols
The following safety and electrical symbols may be used on this product:
Symbol Description Indication
Black graphical
symbol inside a
yellow triangle
with a black
triangular band
This is a warning symbol. The
graphic in this symbol is used to
alert the user to potential hazards.
Black graphical
symbol inside a
red circular band
with a red diagonal
bar
This is a prohibition symbol. The
graphic in this symbol is used to
alert the user to actions that shall
not be taken or shall be stopped.
White graphical
symbol inside a
blue circle
This is a mandatory action symbol. It
is used to indicate that an action
shall be taken to avoid a hazard.
Black graphical
symbol inside a
yellow triangle
with a black
triangular band
This is the general warning sign.
Failure to heed the safety
precautions could result in personal
injury.
White graphical
symbol inside a
blue circle
This is the general data loss or
property damage symbol and is not
related to personal injury. Failure to
heed these precautions can result in
irreparable damage to property or
permanent data loss.
Symbol Indication
Alternating current
Earth terminal or ground
Protective conductor terminal
Fuse
Power on
Power off
8