Fluigent NIFS User manual
N
I
F
S
USER’S
MANUAL
ON
NVASIVE
LOW
ENSOR
PRECAUTIONS
Do not place the product in an unstable location. Place it in a location
with a level surface and a strong and stable support.
Do not use other power supply than the one provided, it has been
carefully selected to meet the power requirements of the NIFS in all
configurations and to comply with all safety standards.
Aria is a perfusion system which automates perfusion or timed
injection protocols. It allows for the sequential delivery of up to
10 different solutions at the desired flow rate into a microfluidic
chip, perfusion chamber or petri dish.
Do not open or dismantle the NIFS device.
Please refer all servicing to after-sales service
department (support@fluigent.com)
Prevent any objects or liquid from entering the NIFS device, this may
cause a short-circuit failure or other malfunction. Failing to respect
this advice would:
• Expose you to direct current/voltage in case the device is under
voltage which may lead to severe damages
• Void device's warranty
• Discharge our company from any liability regarding physical or
device damages.
3
WARRANTY TERMS
This warranty is granted by Fluigent and applies in all countries.
Your Fluigent product is guaranteed for one year from the date of delivery at your
laboratory for defects in materials and workmanship. If found to be defective within
the warranty period, your Fluigent product will be repaired or replaced free of charge.
What This Warranty Does Not Cover
This warranty does not cover routine maintenance, or damage resulting from the failure
to maintain the product in accordance with instructions provided by Fluigent. This
warranty also does not cover damage that arises from accidental or intentional misuse
or abuse, alteration or customization, or repair by unauthorized persons.
How to Get Your Device Serviced
If something goes wrong, contact the Fluigent dealer from whom you purchased your
product. Arrange a mutually convenient time for a Fluigent service representative to
discuss the problem and find a solution to fix the issue. In case more action needs
to be taken, the system will be shipped to Fluigent offices (for no additional cost, if it
is under warranty).
The warranty conditions are:
• Only use cables provided by Fluigent.
• Prevent foreign objects or liquids from being in contact with the F-OEM, the
P-OEM or the PX.
• Prevent foreign objects from entering the NIFS.
• Do not open the NIFS. Refer all servicing to the after-sales service department
(support@fluigent.com).
• Do not place the product in an unstable location. Place it in a location with a
level surface and a strong and stable support.
• Respect the pressure compatibility (max 2 bar).
• Respect the temperature compatibility (from 5°C to 50 °C).
• The customer is responsible for the gas used with the NIFS. It is recommended
to put an air filter to protect the sensor.
• The customer is responsible for the use of the NIFS with any product that is
not from Fluigent.
For specific use, please contact our Support team at support@fluigent.com
INTRODUCING NIFS 5
GENERAL INFORMATION 6
NIFS DESCRIPTION 7
C 7
P 8
USING THE NIFS WITH PNEUMATIC CONTROLLERS 9
NIFS F-OEM 9
NIFS PX / P-OEM 10
FLUIGENT SDK AND SOFTWARE 11
SDK ( ) 11
OGEN 11
START WORKING 12
Q 12
H 12
U 13
FAQ 13
HARDWARE SPECIFICATIONS 14
TECHNICAL SUPPORT 14
NOTES 15
TABLE OF CONTENT
5
The NIFS is dedicated to flow rate measurement and control when
combined with Fluigent pressure controllers. It allows for pressure-
based flow rate control without contact with the liquid.
For applications that require a contamination-free environment,
several types of fluids, continuous operation with slow flow response
time, and flow rates ranging from 100 µL/min to 10 mL/min, we
recommend the NIFS.
INTRODUCING NIFS
This manual will show you how to assemble the NIFS. It will describe
the product’s functionalities and will teach you how to connect and
use it with its required components.
6
GENERAL INFORMATION
The NIFS allows flow rate measurement over the range of 100 µL/min
to 10 mL/min. The liquid flow rate delivered is computed from the
measurement of the gas flow rate sent to the reservoir.
Measure principle
Schematic NIFS setup
7
When purchasing a NIFS [1], you receive one ready-to-use flow sensor
with one electric cable [2] (USB-C to USB-A) and an adaptor [3] (USB-A
to micro-USB) to connect it to the software. The sensor consists of
sensing elements and electronics integrated into a compact case.
Standard M3-sized screws can be used for fixing the device.
C
Here are the PN to order related accessories (please refer to the
following paragraphs for more details):
[P-CAP-L-2] 2 mL reservoir + P-CAP lock + male luer integral lock ring
[P-CAP-S-15] 15 mL reservoir + P-CAP screw + male luer integral lock ring
[P-CAP-S-50] 50 mL reservoir + P-CAP screw + male luer integral lock ring
[P-CAP-L-2-PCK] 2 mL reservoir + P-CAP lock + 80 cm OD 4 mm tubing + male luer
integral lock ring
[P-CAP-S-15-PCK] 15 mL reservoir + P-CAP screw + 80 cm OD 4 mm tubing + male
luer integral lock ring
[P-CAP-S-50-PCK] 50 mL reservoir + P-CAP screw + 80 cm OD 4 mm tubing + male
luer integral lock ring
NIFS DESCRIPTION
1
2
3
8
DESCRIPTION
C
Reservoirs of three volumes are available with the NIFS: 2 mL, 15 mL
and 50 mL. In order to function properly, the NIFS requires 15 mL and
50 mL reservoirs with a P-CAP screw (a P-CAP lock is not compatible
with the use of the NIFS for these volumes).
The NIFS has two (2) pneumatic ports that are compatible with 4 mm
OD (outer diameter) tubing. To get started, FLUIGENT can provide
different kits that include tubing, and a male luer integral lock ring.
1Cut the OD 4mm tubing to the desired length, leaving a square-cut
face.
2Insert the tubing into the receiving port and press as far as it will go.
3To check the tightness of the connection, pull gently on the tubing: it
should slightly move but stay fitted in the rubbing part of the port.
4Do the same thing on the 2nd port.
5To disconnect the NIFS, push on the rubber part while pulling the
tubing out of the port for each side.
P
P
C NIFS
9
If using the Fluigent F-OEM [1], users can directly connect the NIFS
[2] to the pressure module using the micro-USB connector [3]. They
can also connect the NIFS to the integration board or the computer
using the USB-A connector. It will be automatically detected (the LED
near the USB-C socket becomes green).
NIFS F-OEM
USING THE NIFS WITH
PRESSURE CONTROLLERS
1
2
3
10
NIFS AND PRESSURE CONTROLLERS
If using the Fluigent PX [1] or
P-OEM [2], users can connect
the NIFS to the computer
using the USB-A connector [3].
It will be automatically
detected (the LED near the
USB-C socket becomes green).
Precaution: To use a NIFS with a P-OEM, a male luer integral lock
ring is required, to adapt the P-OEM outlet tubing to 4 mm OD
tubing.
NIFS PX / P-OEM
1
3
2
11
The NIFS is fully supported by Fluigent SDK. It has been ported to the
most popular programming language within the instrumentation
field (LabVIEW, C++, C# .NET, Python, and MATLAB).
This SDK merges all Fluigent pressure controllers and sensor
instruments and provides an advanced regulation loop. Specific
functions have been implemented for the NIFS:
fgt_get_sensorValue
fgt_get_sensorBypassValve and fgt_set_sensorBypassValve
To read all functions and the user manual, visit the following webpage:
https://github.com/Fluigent/fgt-SDK
Fluigent OxyGEN software supports the NIFS with version 2.2.0 or
higher. The sensors will be identified and the same level of features of
our end-user products are available.
For more information, visit the OxyGEN webpage here: https://www.
fluigent.com/research/software-solutions/oxygen/
SDK (S D K)
FLUIGENT SDK AND
SOFTWARE
OGEN
12
Q
START WORKING
1First, insert the NIFS between the pressure controller and the
reservoir with the correct tubing.
2Connect the NIFS to the computer for use with the PX, the P-OEM or
the F-OEM, or directly connect it to the F-OEM.
3The NIFS is now ready for your application.
Here is a quick setup guide to get your NIFS up and running.
H
The NIFS can be used in a large range of temperature, but some
elements need to be taken into account:
- The tubing between the pressure controller and the NIFS and the tubing between
the NIFS and the reservoir should be < 1 m to optimize response time.
- Do not touch the NIFS when operating for measurement stability.
- Performance is not guaranteed for temperatures below 15°C or beyond 37°C.
- The temperature should remain constant during operation.
- It’s recommended to use an air filter to protect the sensor.
- Calibration is required for gases other than clean air.
- The regulation performance is not guaranteed for liquids which flow rate is not
linear with the pressure applied (ex: alginate) but measurement is not affected.
13
START WORKING
U
When air flows in the NIFS, it can go through two paths. One has a
sensor that measures the air flow rate and the other has a bypass
valve. If pressure is applied, the sensor reduces the passage of air,
which increases the response time.
When the NIFS is used for regulation or when a manual pressure
command is made, the algorithm automatically optimizes the
response time/stability ratio by controlling the opening/closing of the
valve to facilitate the passage of air.
Please note that when the valve is opened, the LED near the USB-C
socket becomes blue (when the valve is closed, the LED is green) and
the flow rate monitoring is stopped as no air goes through the sensor.
Manual control of the opening of the valve is possible via the dedicated
function of the SDK or the dedicated button on OxyGEN.
FAQ
Is it possible to use the NIFS with other manufacturer’s pressure
controllers?
Flow rate monitoring is possible, but performance is not guaranteed.
14
HARDWARE
SPECIFICATIONS
Characteristics Value Unit
Dimensions 99.85 x 45.0 x 33.5 mm x mm x mm
Weight 117 g
Pneumatic fittings 4 mm OD
Maximum pressure 2bar
TECHNICAL SUPPORT
Email our technical support team : support@fluigent.com
Or call our technical support team directly
Fluigent S.A.S +33 1 77 01 82 65
Fluigent Inc. +1 (978) 934 5283
Fluigent GmbH +49 3641 277 652
Still have a question?
Email us at : contact@fluigent.com
15
NOTES
VERSION
NOV. 2022
Table of contents
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