Genetix ClonePix FL User manual

07MAN1054.A2
Effective Date: 27-May-11
ClonePixTM FL
Robot Manual

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Contents
Table of Figures .................................................................................................4
Introduction to ClonePix FL..............................................................................5
Technical Specifications ...................................................................................6
External Dimensions and Weight.............................................................................................6
Compressed Air Supply............................................................................................................6
Operating Environment.............................................................................................................6
Electrical Supply........................................................................................................................7
Electrical Connections..............................................................................................................8
Symbols on Equipment.............................................................................................................8
PC Connections.........................................................................................................................9
Filter options ............................................................................................................................10
Robot Enclosure ..............................................................................................11
Installation........................................................................................................12
Layout of ClonePix FL and Ancillary Equipment .................................................................12
Source and Destination Plate Stackers.................................................................................13
Connecting the Wash Bottles.................................................................................................14
Connecting the Ethanol Bottles .............................................................................................14
Camera Alignment ...................................................................................................................15
ClonePix FL Panel ...................................................................................................................15
Health and Safety.............................................................................................16
Important Note .........................................................................................................................16
Transport and Storage ............................................................................................................16
Lifting Points............................................................................................................................16
External Covers .......................................................................................................................16
Safety Features ........................................................................................................................17
Electrical Safety.......................................................................................................................18
Drive Safety..............................................................................................................................18
Hot Air/Halogen Dryer .............................................................................................................18
Noise Levels.............................................................................................................................18
Service and Maintenance........................................................................................................18
Initial Operation................................................................................................19
System Features ......................................................................................................................19
Pre-Power-Up Checklist..........................................................................................................19
Power-Up Procedures .............................................................................................................20
Shutdown procedure...............................................................................................................20

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General Maintenance.......................................................................................21
Regular Maintenance ..............................................................................................................21
General Precautions................................................................................................................22
Cleaning Procedures...............................................................................................................22
Removable Parts................................................................................................................. 22
Cleaning of Instrument Interior ............................................................................................ 24
Cleaning of System Fluid (Sterile Water) Supply ................................................................ 24
Incoming Compressed Air Supply ....................................................................................... 24
Automated Picking Pin Cleaning......................................................................................... 25
Sterility Validation Tests ...................................................................................................... 25
Changing the Picking Head ....................................................................................................26
Remove Head:..................................................................................................................... 26
Replace Head:..................................................................................................................... 26
Changing or Replacing Picking Pins.....................................................................................26
Replacement Parts and Optional Extras........................................................27
Replacement Parts ..................................................................................................................27
Optional Extras ........................................................................................................................27
Reagents and Supplies....................................................................................28
CloneDetect Detection Reagents...........................................................................................28
CloneMatrix ..............................................................................................................................29
CloneMedia and XP Media ......................................................................................................29
PetriWell Cell Culture Plates ..................................................................................................30
Other .........................................................................................................................................31
Troubleshooting...............................................................................................32
Common Problems..................................................................................................................32
Glossary of Terms ...........................................................................................33
Index .................................................................................................................36
Contact Details.................................................................................................37

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Table of Figures
Figure 1: ClonePix FL electrical connections ........................................................................... 8
Figure 2: ClonePix FL PC connections ...................................................................................... 9
Figure 3: ClonePix FL layout and dimensions ........................................................................ 12
Figure 4: Stacker orientation .................................................................................................... 13
Figure 5: Stackers and cassettes ............................................................................................. 14
Figure 6: Connecting the wash bottles.................................................................................... 14
Figure 7: ClonePix FL Panel...................................................................................................... 15

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Introduction to ClonePix FL
Manufactured by Genetix Ltd (UK)
ClonePix™ FL is a dedicated solution for imaging, identification, and selective picking of
antibody or protein secreting cells. The technology is based on quantitative fluorescence
detection. The system is also compatible with direct fluorescence (e.g. GFP expression).
• Automated delivery and lid removal of 1 well and 6 well micro-plate format plates to on
board imaging station and dispersal of picked colonies to 96 well plates.
• Fluorescent imaging using a choice of five filter sets.
• Colonies selected on the basis of size, shape and proximity to neighbors.
• Colonies ranked based on fluorescent intensity of the colony or the secreted product.
• Automated selective picking using high precision picking pins with proprietary cleaning
routine between each pick.
Intuitive Windows
®
-based software allows you to easily set up multiple picking processes and
analyze the results. For detailed information about the software please see the ClonePix FL
Software Application Manual.
Note: ClonePix FL is strictly for research use only and is not intended or recommended for the
diagnosis of disease in humans or animals.
If the robot is used in a manner not specified in this manual the protection provided by the
equipment may be impaired.

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Technical Specifications
External Dimensions and Weight
Size (excluding ancillary equipment): 1010mm (width) x 900mm (depth) x 1495mm (height)
Weight: Robot - 350 kg (maximum)
Compressor - 60 kg
Compressed Air Supply
Minimum Pressure: 6 bar
Minimum volume: 80L/min
Operating Environment
Indoor use only
Temperature: 10°C to 40°C
Humidity: 20 to 80% non-condensing
Altitude: Up to 2000M
Mains supply: +/- 10% Rated Voltage
Transient overvoltage: Installation Category (Overvoltage category) II
Rated pollution: Pollution degree 2

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Electrical Supply
European:
Voltage: 210 - 250V AC 45-66 Hz single phase
Power: Robot - 2500W
Halogen Heater - 300W
Connections: IEC Input - Robot Mains / Halogen Heater
IEC Output - PC
IEC Output - Monitor
Fuses: Input - F3 – T10A - Robot / Halogen Heater
Output - F2 - T5A - Computer and Monitor
Compressor: 230V AC 50Hz 3.4A
USA/Japan:
Voltage: 100 – 125V AC 45-66 Hz single phase
Power: Robot -1250W
Halogen Heater - 300W
Connections: IEC Input - Robot Mains / Halogen Heater
IEC Output - PC
IEC Output - Monitor
Fuses: Input - F3 - T10A - Robot / Halogen Heater
Output - F2 - T5A - Computer and Monitor
Compressor: 120V AC 60Hz 7.5A

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Electrical Connections
Figure 1:
ClonePix FL electrical connections
Symbols on Equipment
Symbol Meaning
Beware moving parts.
Refer to Robot Manual for operating instructions.
2007
The year of manufacture is given on the serial number label which is located
on the right side of the unit.
European Economic Area (EEA)
This mark on the product indicates compliance with the following EEC Directives:
- 98/37/EEC, relating to The Supply of Machinery (Safety) Regulations 1992
- 89/336/EEC with amendments 92/31/EEC, relating to Electromagnetic Compatibility
- 73/23/EEC, the Low Voltage Directive

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PC Connections
Figure 2:
ClonePix FL PC connections

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Filter options
Filter set Excitation Emission
Cat. No. Suitable for: Wavelength (nm) Filter
Position
Wavelength (nm) Filter
Position
X4990 CFP
AF430
440
(Royal Blue)
4 505
(Cyan)
4
X4991
(standard)
CloneDetect FITC*
Fluorescein
GFP
AF488
470
(Blue)
3 535
(Green)
3
X4992 YFP 500
(Cyan)
2 550
(Yellow)
2
X4993 CloneDetect 549*
R-phycoerythrin
Rhodamine
AF546
530
(Green)
1 590
(Red)
1
X4994 CloneDetect 649*
Cy5
FMAT Blue
AF647
622
(Red)
6 700
(Far Red)
6
Notes:
• *Refer to Reagents & Supplies section for details on how to order CloneDetect reagents.
• White light imaging uses excitation filter position 7 and emission filter position 5.