Gilson 322 User manual

User's Guide
322 HPLC Pump

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322 HPLC PUMP | USER’S GUIDE
TABLE OF CONTENTS
SAFETY | 7
Electronic and Hazard Symbols | 8
Safety Notices | 8
Lifting | 9
Voltage | 9
Solvents | 9
Replacement Parts | 9
CHAPTER 1 | INTRODUCTION | 11
Description | 12
Unpacking | 16
Technical Specifications | 17
Customer Service | 18
CHAPTER 2 | INSTALLATION | 19
Electrical Connections | 20
Cabling for TRILUTION LC Control | 21
Hydraulic Connections | 21
CHAPTER 3 | OPERATION | 25
Front Panel and Startup | 26
Priming | 26
Pump Operating Parameters | 29

TABLEOFCONTENTS |322 HPLC PUMP
TABLE OF CONTENTS
CHAPTER 4 | MAINTENANCE | 31
Maintenance Overview | 32
Cleaning | 32
Pump Head | 33
Piston Seals | 37
Piston and Bellows | 38
Piston Rinsing Chamber | 39
Reassemble Pump Head | 40
Check Valves | 41
Filters | 43
Maintenance Procedures | 45
Fuse Replacement | 46
CHAPTER 5 | TROUBLESHOOTING | 47
Troubleshooting | 48
Repair and Return Policies | 49

322 HPLC PUMP | USER’S GUIDE
TABLE OF CONTENTS
APPENDIX A | REPLACEMENT PARTS | 51
322 HPLC Pump | 51
Hydraulic | 51
Pump Heads | 52
Electrical | 53
Miscellaneous (Tools, Power Cords, Cables, etc.) | 53
Service Parts | 54
APPENDIX B | COLUMN HOLDER | 55
Installation | 55
Part Numbers | 56
APPENDIX C | INJECTOR | 57
Injection Valve Holder | 58
Connection to Injector | 58
Part Numbers | 58
APPENDIX D | REFERENCE INFORMATION | 59
Liquid Contact Materials | 59
Solvent Miscibility | 61

Chapter1|SaFetY
The instrument is intended to be used in a laboratory by trained technical personnel. For safe
and correct use, both operating and service personnel should follow the instructions contained
in this guide when installing, cleaning, and maintaining the instrument.
The following safety precautions must be observed during all phases of operation, service, and
repair of the instrument. Failure to comply with these precautions or with specific warnings
elsewhere in the user’s guide violates safety standards of design, manufacture, and intended
use of the instrument. Gilson assumes no liability for the customer’s failure to comply with
these requirements.
The instrument has been certified to safety standards required in Canada, Europe, and the
United States. Refer to the rear panel label on the instrument or the Declaration of Conformity
document for the current standards to which the instrument has been found compliant.
IN thIS Chapter
●Electronic and Hazard Symbols | 8
●Safety Notices | 8
●Lifting | 9
●Voltage | 9
●Solvents | 9
●Replacement Parts | 9
7
322 HPLC PUMP | USER’S GUIDE

8SAFETY |322 HPLC PUMP
SAFETY
Electronic and Hazard Symbols
The following electronic and hazard symbols may appear on the instrument:
Symbol Explanation
~Alternating current
Direct current
Protective conductor terminal
|Electrical power ON
OElectrical power OFF
Caution, risk of electric shock
Caution
Fuse
Safety Notices
The following safety notices may appear in this document:
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, may result in
serious injury
CAUTION
CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury
NOTICE
NOTICE indicates a potentially hazardous situation which, if not avoided, may result in
equipment damage

9
322 HPLC PUMP | USER’S GUIDE
SAFETY
Lifting
The instrument exceeds the weight one person can lift safely. Two or more people are required
to lift the instrument safely. Refer to the Technical Specifications for the weight. Always lift the
instrument from the base and follow any unpacking instructions provided with the instrument.
Voltage
Access to the rear panel is necessary. The instrument must be detached from all voltage
sources before service, repair, or exchange of parts. For normal operation, the instrument is to
be grounded through the AC line cord and power supply provided. Failure to do so can result
in a potential shock hazard that could result in serious personal injury.
Use only fuses with the rated current and of the specified type as listed on the rear panel label
on the instrument. The instrument must only be operated with the voltage specified on the rear
panel label of the instrument and with the grounded AC line cord and power supply provided.
Solvents
Observe safe laboratory practices when handling solvents. If working with hazardous solvents
or flammable liquids, ensure that there is proper ventilation and that adequate protection such
as safety glasses, gloves, and protective clothing are used.
If dangerous liquids are used, precautions should be taken to limit potential hazards from leaks
and/or spillage through the use of a non-flammable tray or use of a fume hood, etc.
If there is the potential of explosive gases being developed, a fume hood or other means should
be used to safely manage that risk.
Refer to the Material Safety Data Sheets for the solvents before use.
Replacement Parts
Be sure to use only replacement parts mentioned in the user’s guide. Do not repair the instrument
or change parts not listed in the user’s guide. If it is necessary to replace parts not listed, please
contact your local Gilson representative.

11
322 HPLC PUMP | USER’S GUIDE
Chapter 1
CHAPTER1|INTRODUCTION
IN THIS CHAPTER
●Description | 12
●Unpacking | 16
●Technical Specifications | 17
●Customer Service | 18

12 INTRODUCTION |322 HPLC PUMP
DESCRIPTION
Description
The 322 HPLC Pump is a multi-solvent pump for analytical to
semi-preparative high performance liquid chromatography
(HPLC). It accommodates flow rates from 0.15 mL/min
up to 30 mL/min at pressures up to 8700 psi (600 bar).
It includes a built-in, high-pressure adjustable volume
dynamic mixer (AVDM) allowing users to perform a binary
gradient with a single unit. The 322 Pump is controlled by
TRILUTION® LC Software as part of an HPLC system.
Hydraulic Components
The hydraulics are revealed by opening the removable door.
The pre-mounted, principal hydraulic components are pump
heads, pressure module, dynamic mixer, purge valve, and
outlet filter. Two reciprocating pump motors are contained
within the body of the pump. The tops of the pump motors
protrude through the front panel. There is a drip tray at the
bottom of the pump (see Drip Tray on page 22). Solvent
bottles can be placed in the tray on the top of the pump
(see Solvent Bottle Tray on page 23).
The right-hand motor pumps a solvent (normally ‘A’) via the pressure module to the dynamic
mixer and the left-hand motor pumps another solvent (normally ‘B’) directly to the dynamic
mixer.
Pressure
Module
(PM)
Adjustable
Volume
Dynamic
Mixer
(AVDM)
Purge
Valve
(PV)
Pump
Head
(L)
Pump
Head
(R)
Outlet
Filter
(OF)
Figure 2
Hydraulic Components
Purge
Line
Figure 1
322 HPLC Pump

13
322 HPLC PUMP | USER’S GUIDE
DESCRIPTION
PUMP HEADS
Two interchangeable pump head types are available:
●H1: flow rate from 0.15 to 15 mL/min, pressure up
to 600 bar (8700 psi)
●H2: flow rate from 0.30 to 30 mL/min, pressure up
to 300 bar (4350 psi)
Each pump head has a solvent inlet port, a solvent
outlet port, an inlet port to the rinsing chamber,
an outlet port from the rinsing chamber, and a
reciprocating piston.
The piston seal and the bellows are inside the pump
head. The solvent inlet port and the solvent outlet
port are fitted with connectors containing the check
valves. All of these items can be serviced by the user.
The pump heads are pre-mounted directly onto the
tops of the driving mechanisms, which have the same
axis as the piston motors, so pump heads may be
changed with relative ease. The pump heads must
be dismounted for routine servicing purposes (for
example, changing a piston seal). Figure 3
H2 Pump Head

14 INTRODUCTION |322 HPLC PUMP
DESCRIPTION
ADJUSTABLE VOLUME DYNAMIC MIXER (AVDM)
The adjustable volume dynamic mixer (AVDM) performs high-pressure
mixing of the solvents coming from channels A and B, and (optionally)
solvent coming from another pump or pumps. The dynamic mixer
volume is continuously adjustable between 0.20 mL and 2.20 mL. When
the dynamic mixer is operating, the indicator (green LED) lights up.
PRESSURE MODULE (TRANSDUCER)
The pressure module (transducer) integrates:
●a dampener that minimizes the pulsations created by the reciprocating
action of the pump motor.
●a built-in transducer that detects the pressure in this part of the
hydraulic circuit.
Figure 5
Pressure Module (Transducer)
PURGE VALVE
After mixing, the next component in the hydraulic circuit is the
purge valve, which is used to manually direct the mobile phase
towards the outlet filter or divert to the drain.
OUTLET FILTER
The outlet filter protects the injection valve and the column. The
outlet filter can be replaced by the user.
Figure 4
AVDM
Figure 6
Purge Valve and Outlet Filter

15
322 HPLC PUMP | USER’S GUIDE
DESCRIPTION
Front Panel
The 322 Pump must be controlled by external software, such as
TRILUTION LC. The 322 Pump has a front panel to control power or
indicate errors.
Rear Panel
Figure 8
322 HPLC Pump Rear Panel Diagram
All electrical connections are made on the rear panel. The
rear panel of the 322 Pump houses the main power switch, a
GSIOCport, unit ID selector, and baud rate selector.
The pump has two ON/OFF switches: one on the power
receptacle on the rear panel and the other on the front panel
above the LEDs.
!
POWER
ON
REMOTE
ON/OFF
Figure 7
322 HPLC Pump
Standby Panel
Figure 9
ON/OFF Switches on Rear Panel

16 INTRODUCTION |322 HPLC PUMP
UNPACKING
Unpacking
The pump is delivered with most major components already assembled. Keep the original
container and packing assembly so the unit may be shipped safely, if necessary.
Carefully unpack the pump and its accessories from the carton.
CAUTION
Because of its weight, you should take special care when handling the large carton. Pump
modules are heavy and should be lifted from the carton with care, by two people. Instructions
describing the unpacking procedures can be found on and in the carton.
It is necessary for two people to lift the 322 Pump out of the box, using the straps provided.
The 322 Pump weighs approximately 26kg (57 lbs.). To avoid personal injury and for general
safety, if moving or lifting the system, always get another person to assist you. Always follow
local health and safety regulations.
Standard Equipment
After the instrument and the accessories have been unpacked, you should have the following:
●322 HPLC Pump with H1 or H2 Pump Heads
●An accessory kit that includes:
○Glass Priming Syringe
○Solvent Bottle Tray
○GSIOC Cable
○Wrenches
○Glass Bottle
○Tubing and Fittings
○Bellows Mounting Tool
○Extra Fuses
○Power Cord
○Plumbing Kit for Piston Rinsing Chambers
DOCUMENTATION
Documents are provided on the supplied 322 HPLC Pump Documentation USB.
Optional Accessories
●Column Holder
●Manual Injection Valve Holder
For part numbers and installation instructions for the optional accessories, refer to the dedicated
appendices.

17
322 HPLC PUMP | USER’S GUIDE
TECHNICAL SPECIFICATIONS
Technical Specifications
Please be aware of the following before operating the pump.
NOTICE
Changes or modifications to the pump not expressly approved by Gilson could void the
warranty.
This instrument complies with part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) This instrument may not cause harmful interference, and (2) this instrument must
accept any interference received, including interference that may cause undesired operation.
Shieldedcables must be used with the pump to ensure compliance with the FCC Class A limits.
322 HPLC Pump
Specification Description
Pump Type Binary Gradient Pump
Hydraulic System Reciprocating Piston Pump
Pump Head H1: Up to 15 mL/min
H2: Up to 30 mL/min
Flow Rate
Range - Single Head
H1: 0.015–15 mL/min
H2 : 0.030–30 mL/min
Range (Recommended) - Two Heads
H1: 0.15–15 mL/min
H2 : 0.30–30 mL/min
Increment
0.001 mL/min
Flow Accuracy ± 2%
Flow Precision ≤ 0.7% RSD
Gradient
Solvents
Two
Formation
High Pressure Mixing with Adjustable Volume Dynamic Mixer (AVDM)
Composition Increment
0.1%
Gradient Accuracy ± 2%
Gradient Precision ≤ 0.7% RSD
Operating Pressure H1: 5–600 bar (70–8700 psi)
H2: 5–300 bar (70–4350 psi)
TECHNICAL SPECIFICATIONS CONTINUED ON PAGE 18

18 INTRODUCTION |322 HPLC PUMP
TECHNICAL SPECIFICATIONS
322 HPLC Pump
Specification Description
Compressibility
Compensation Settable Compensation Range 0–2000 Mbar-1
Piston Seal Wash Pump Head Inlet and Outlet Ports to/from a Rinsing Chamber
Priming Manual with Built-in Prime/Purge Valve via Control Software or Syringe
Liquid Contact Materials
316L Stainless Steel, Sapphire, Ceramic, HDPE, PTFE, Ruby, Titanium,
FEP, PCTFE, PEEK, ETFE
For more information, refer to Liquid Contact Materials on page 59.
Control and
Communication
Communication
GSIOC (Gilson Serial Input Output Channel)
Software Control
TRILUTION® LC Software
Electrical
Line Voltage
90 –260 VAC
Frequency
50 or 60 Hz
Power Consumption
150 W
Environmental
Operating Temperature
4°C to 40°C
Operating Humidity
15%–80%
Operating Altitude
Up to 2000 m (81 kPa or 604 mmHg)
Physical
Dimensions (W x D x H)
26 x 45.7 x 30.3 cm (10.2 x 18 x 11.9 in.)
Weight
25.9 kg (57 lbs.)
Shipping Weight: 30.4 kg (67 lbs.)
Contact [email protected]om for the methods and conditions that were used to obtain
technical specifications.
Customer Service
Gilson, Inc. and its worldwide network of representatives provide customers with the following
types of assistance: sales, technical support, applications, and instrument repair.
If you need assistance, please contact your local Gilson representative. Specific contact
information can be found at www.gilson.com. To help us serve you quickly and efficiently,
please refer to Repair and Return Policies on page 49.

19
322 HPLC PUMP | USER’S GUIDE
Chapter 2
Chapter2|INStaLLatION
This chapter describes the minimum connections required for operation (including procedures
for preparing piston rinse lines). The pump must be set up and installed in the order described
in this chapter to avoid damaging the system.
TRILUTION® LC Software is the recommended controller for the pump.
The pump is delivered with the main hydraulic components installed. Solvent bottles can be
placed in the removable trays atop the pump, on the bench, or on the floor.
IN thIS Chapter
●Electrical Connections | 20
●Cabling for TRILUTION LC Control | 21
●Hydraulic Connections | 21

20 INSTALLATION |322 HPLC PUMP
eLeCtrICaL CONNeCtIONS
Electrical Connections
Plug the power cords into the pump and all associated devices before making the communication
or hydraulic connections. Power is needed to prime the pump and piston rinse chambers prior
to use.
Rear Panel
All electrical connections are made on the rear panel. Also present on the rear panel are the
fan’s ventilation slots, which must never be obstructed in any way.
Figure 10
322 HPLC Pump Rear Panel Diagram
GSIOC ID
The GSIOC ID may be changed when the pump is
being controlled from a computer. The configured
ID on the pump must correspond to that set in the
controlling software.
The GSIOC identification number (ID) that you set
must be unique for each item of Gilson equipment.
The GSIOC ID (0–9) is set mechanically for the
322 Pump using the SW1 (left) selector on the rear
panel.
BaUD rate
The baud rate (9600, 19200, or External) is set mechanically
using the SW2 (right) selector on the rear panel.
External clock control should be selected for the 322 Pump
running under computer control. Internal is used when the
connected device does not provide a clock source, in which case
you have to select an appropriate baud rate.
VOLtaGe SeLeCtION
The pump can be connected to an AC power supply of 110/120V or 220/240V. Automatic
selection of the operating voltage takes place on the power supply board.
pOWer CONNeCtION
Plug the power supply cord that you received with the pump into the socket on the power
receptacle (standard 3-pin connector) and to a suitable source of power.
Figure 11
GSIOC ID
(0–9)
Figure 12
Baud Rate
0 = External
1 = 9600 Baud
2 = 19200 Baud