Instruction Manual
www.hannainst.com
DearCustomer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the
chemical test kit. It will provide you with the necessary
information for correct use of the kit.
Remove the test kit from the packing material and examine
it carefully to make sure that no damage has occurred
during shipping. If there is any noticeable damage, notify
your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
•HI 3856-0 Reagent, packets (100 pcs);
•1 long path color comparator cube.
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 3856
Copper
Ultra Low Range
Test Kit
ISTR3856R1 02/00-L PRINTED IN ITALY
Copper is an essential trace element in human diet (the
daily requirement is around 2.0 mg) and a factor in plant
metabolism. Copper salts are widely used in water supply
systemstocontrolbiologicalgrowth inreservoirs.Corrosion of
copper alloys in pipe fittings on the other hand may
introduce considerable quantities into the water supplies.
Note: mg/L is equivalent to ppm (parts per million).
Range 0 to 0.25 mg/L (ppm) as Copper
Smallest Increment 0.05 ppm
Analysis Method Colorimetric,bicinchoninate
Sample Size 25 mL
Number of Tests 100
Case Dimensions 230x59x70 mm (9.0x2.3x2.8")
Shipping Weight 180 g (6.3 oz.)
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONSSPECIFICATIONS
SPECIFICATIONS
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USESIGNIFICANCE AND USE
SIGNIFICANCE AND USE
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTIONCHEMICAL REACTION
CHEMICAL REACTION
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
• Fill the long path comparator cube with the sample to
the 25 mL mark.
• Add 1 packet of HI 3856-0 reagent.
• Replace the cap and mix by inverting the cube several
times.
• Wait 45 seconds to allow color to develop.
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONSINSTRUCTIONS
INSTRUCTIONS
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Health and Safety
Data Sheet before performing this test.
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETYHEALTH AND SAFETY
HEALTH AND SAFETY
REFERENCESREFERENCES
REFERENCESREFERENCES
REFERENCES
Adapted from Nakano S., Yakugaku Zasshi,
19621962
19621962
1962,82 486-491
[Chemical Abstract, 58 3390e (1936)].
Copper salts react with bicinchoninate reagent to form a
purple product in neutral buffered condition. The amount of
colordeveloped isproportionalto the concentrationofcopper
present in the aqueous sample.
• Determine which color best matches
the solution in the cube and record
the result as mg/L (ppm) of Copper.
• It is better to match the color with a white sheet at
about 10 cm behind the comparator.
Note: To measure Copper in the 0-2.5 ppm range, use the
HI 3847 Copper Test Kit.
