Instruction Manual
www.hannainst.com
Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instruction manual carefully before using
the test kit. It will provide you with the necessary information
for correct use of the kit. If you need additional information,
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable damage,
notify your Dealer or the nearest Hanna office immediately.
Each kit is supplied with:
•Ammonia Reagent 1 for Fresh Water, 1 bottle with
dropper (20 mL);
•Nessler Reagent, 1 bottle with dropper (20 mL);
•1 checker disc (containing the 38049 disc);
•2 glass vials with caps;
•1 plastic pipette (3 mL).
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 38049
Ammonia
Test Kit
for Fresh Water
with Checker Disc
ISTR38049R3 01/05
Ammonia is commercially used as a fertilizer, either as such
or in the form of compounds. Its presence in raw surface
waters indicates animal or plant microbiological decay and
above certain critical levels it is toxic to fish.
The Hanna test kit measures concentration of ammonia-
nitrogen up to 3 ppm in fresh waters, employing the
Nessler colorimetric method.
Note:
mg/L is equivalent to ppm (parts per million).
Range 0.0 to 3.0 mg/L (ppm) as NH3-N
Smallest Increment 0.1 mg/L (ppm) NH3-N
Analysis Method Colorimetric
Sample Size 5
mL
Number of Tests 100
Case Dimensions 165x150x38 mm (6.5x5.9x1.5")
Shipping Weight 248 g (8.7 oz.)
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
•Using the plastic pipette, fill each glass
vial with 5 mL of sample (up to the
mark).
•Insert one of the vials into the left hand
opening of the checker disc. This is the
blank.
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Health and Safety
Data Sheet before performing this test.
Adaptation of the ASTM Manual of Water and Environmental
Technology, D1426-92, Nessler method.
Ammonia reacts with the reagent in basic solution to form a
yellow compound. The absorbance of this colored product is
proportional to the concentration of ammonia-nitrogen present
in the aqueous sample.
•Add 1 drop of Ammonia Reagent 1, replace the cap
and mix.
•Add 4 drops of Nessler Reagent, replace the cap and
mix.
•Wait for 5 minutes to allow color to
develop. This is the reacted sample.
•Remove the cap and insert
the reacted sample into
the right hand opening
of the checker disc.
•Hold the checker disc so
that a light source
illuminates the samples
from the back of the windows.
5
m
L
x
1
x
4
•Keep the checker disc at a distance of 30-40 cm (12-
16") to match the color. It is better to match the color
with a white sheet on the background. Rotate the disc
while looking at the color test windows and stop when
you find the color match. Read the value in the result
window directly in mg/L (or ppm) of Ammonia-nitrogen
(NH3-N).
•To convert the reading to mg/L of Ammonia (NH3),
multiply it by a factor of 1.214.
For best results: Perform the reading three times and take
the average value (divide by 3 the sum of the three
numbers). Intensely colored samples will make the
color matching difficult and they should be adequately
treated before performing the test. Suspended matter
in large amounts should be removed by prior filtration.
Caution: Ultraviolet radiation may cause fading of colors.
When not in use, keep the disc protected from light,
in a cool and dry place.
Interferences: interference may be caused by hardness
above 1 g/L, iron, sulfide, glycine, various aliphatic
and aromatic amines, organic chloramines, acetone,
aldehydes, alcohols.
SPECIFICATIONS
SIGNIFICANCE AND USE
CHEMICAL REACTION
INSTRUCTIONS REFERENCES
HEALTH AND SAFETY
