HLS TH 21 User manual

Thermo Shakers and
Block Thermostats with
Easy Control MHR 11 – TH 21
Operating Manual
Bedienungsanleitung

2
Content
1. Basic Data.................................................................................... 4
Safety.............................................................................................................4
Warranty .......................................................................................................5
Disclaimer of Warranty .................................................................................5
Important Information .................................................................................5
Environmental conditions .............................................................................6
Temperature Accuracy .................................................................................6
2. First Steps.................................................................................... 6
Setup..............................................................................................................6
Temperatur Setting ......................................................................................7
Operation Shaking ......................................................................................10
3. Assembly of Accessories .............................................................10
Anti-condensation plate BA 24 / 96 ............................................................10
4. Troubleshooting .........................................................................11
5. Maintenance .............................................................................11
Cleaning ......................................................................................................11
Service ........................................................................................................13
6. Technical Data ...........................................................................14
7. Article description blocks & accessories ......................................15
8. Explanations ..............................................................................17

3
Inhaltsverzeichnis
1. Basisdaten..................................................................................18
Sicherheit.....................................................................................................18
Gewährleistung und Garantie ....................................................................19
Haftungsausschluss ....................................................................................19
Wichtige Hinweise .....................................................................................19
Umgebungsbedingungen ...........................................................................20
Temperaturgenauigkeit .............................................................................20
2. Erste Schritte ..............................................................................21
Inbetriebnahme...........................................................................................21
Einstellung der Temperatur.........................................................................21
Einstellung des Schüttlers............................................................................25
3. Montage von Zubehörteilen .......................................................25
Antikondensplatte BA 24 / 96 ....................................................................25
4. Problembehebung .....................................................................26
5. Instandhaltung ...........................................................................26
Reinigung ....................................................................................................26
Service ........................................................................................................28
6. Technische Daten .......................................................................29
7. Artikelbezeichnung Wechselblöcke und Zubehör ........................30
8. Erläuterungen ............................................................................32

4
Basic Data
The HLC by DITABIS Thermo Shakers and Block Thermostats are used for tem-
pering and mixing of solutions in closed reaction tubes and plates. Please pay
attention that the target temperature of the samples should not exceed the
boiling point of the solution, which should be heated. Please consider this
when setting the target temperature via the software of the Thermo Shaker
or Block Thermostat. If there are any uncertainties regarding the liquids,
which should be heated or cooled, please contact DITABIS. The HLC by
DITABIS devices are meant for indoor usage only. Please use only HLC by
DITABIS accessories or accessories recommended by HLC by DITABIS.
Safety
The systems comply with the standards and directives mentioned in the
applicable CE declaration.
Please take note of the following safety measures:
Do only connect the system to an earthed mains power
socket of 230 V, 50 Hz.
Do not place hot blocks on inflammable or not heat-resistant
surfaces.
Make sure to only use containers in the block which are
suitable for the desired temperature range.
If liquid gets into the system, immediately pull out the plug
and contact our service department to ensure complete
safety.
Please do not touch or transport hot systems.
Contact with highly flammable fluids must be avoided.
The ventilation slots of the device must not be blocked, to
ensure the proper ventilation at all times.
Damaged mains cable should only be replaced with equal
ones.

5
Warranty
All functions of the systems have been tested thoroughly. After that, the sys-
tem and the accessories were packed carefully in perfect condition.
If, however, any damages or defects are detected during installation or setup,
please inform DITABIS immediately (see our Terms and Conditions at
www.ditabis.com).
If used under normal laboratory conditions according to the Operating Manu-
al, DITABIS grants a warranty of one year for the material and workmanship,
starting with the day of shipment.
Disclaimer of warranty
In case of unspecified use of the device, the manufacturer assumes no re-
sponsibility.
Important Information
This manual complies with the standards and directives mentioned in the EN
DIN 61010-1 Norm. IQ, OQ and PQ documents are available on request.
The systems are provided with a high-precision self-optimising
temperature controller. Due to the self-optimisation it might oc-
cur that the set temperature is exceeded in individual cases.
Please note, that by heating your samples dangerous gases may
be emitted. In this case, the device must be used with an extrac-
tor hood.
If the Thermo Shakers are operated with a high shaking frequen-
cy, the vibrations of the system might transmit to the under-
ground. Please do consider this when placing the system.
Contact with highly flammable liquids must be avoided. The ex-
changeable block may be hot.
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6
The ventilation slots of the device must not be blocked, because the proper
ventilation of the device must be assured at all times.
Environmental conditions
The ideal area of operation of the devices is achieved at 80% relative air
humidity at most and lays between +3°C - +50°C ambient air temperature.
This range should not be underrun or exceeded.
Temperature Accuracy
Every HLC by DITABIS system is calibrated with a gauged high-precision tem-
perature measuring device to temperature accuracy and reproducibility. This
process is performed at a room temperature of +20°C and a block tempera-
ture of +37°C. The absolute calibration of the heating unit can also be per-
formed for a certain temperature range if the customer desires. HLC by DITA-
BIS reaches a very high accuracy by measuring the block temperature directly
inside the block. It can, however, not be avoided that the cold room tempera-
ture affects the sample temperature in case of high temperature difference
between room and block temperature. The anti-condensation plate reduces
this effect. We recommend using the anti-condensate plate BA 24 / BA 96 or
the tempering tub BT 01 / BT 02, if high temperature accuracy in the sample is
required. When setting a process time, please note that the sample tempera-
ture is always reached a little later than the displayed block temperature.
First Steps
Setup
Plug the mains cable in the IEC socket on the back of the system and connect
it with the 230 V, 50 Hz via an earthed mains power socket.
Changeable blocks

7
At delivery, the changeable blocks are packed separately. Place the blocks on
the tempering plates and take care of the proper alignment of the central
threaded bolt and the two small corner bolts ensuring that the block lays
accurately on the tempering plate. Then attach the block with the short sock-
et screw tightly at the bottom of the tempering plate to get the block fixed for
shaking and to obtain even better temperature accuracy.
Check the tight attachment of the block by pulling it up. If it loosens, please
use the longer screw.
Never start the process without the block being tightly attached. Do
always use two blocks for two-block systems, even if only one of
them is used. This ensures safety as well as a smooth shaking opera-
tion.
Switch on the system with the mains switch on the back of the system, the
actual temperature value is indicated on the display.
Temperature Setting
Actual
value:
After power-on, the current actual
temperature value is displayed.
Display set
value:
Push
Set
. The last entered set value
is displayed and a hyphen appears
on the top left as a marking.
Back to
actual
value:
Push OK and the current actual val-
ue is displayed.
Change set
value:
Push
Set
to get to the set value.
Push Set again, hold it for five sec-
onds and then release the button.
The cursor stands now at the figure
in the middle (1. Digit position).

8
With
Set
and
Start / Stop
you can
navigate to the digit of the set value
which should to be changed.
Change
values:
The digit of the set value is increased
or decreased by pushing +or -.
By exceeding the value 9, the next
decade is increased automatically.
To activate the hundreds digit, ex-
ceed the value 9 on the 2. digit.
By falling below the value 0, the
next-lower decade is selected auto-
matically.
Confirm set
value:
Push OK and the current actual val-
ue is displayed again.
Start heat-
ing process:
If the system heats, a “
: “
emerges.
The green LED lights. If a process
time is preset, the horizontal hyphen
flashes as an
to indicate the preset time.
Push Start / Stop.to start the heat-
ing process. Depending on the heat
output it either flashes or is perma-
nently visible.
Stop heat-
ing process:
.
The “
: “
turns off and the current
temperature actual value is dis-
played. If a process time is preset
and if the process is stopped before
the expiration of this time, the clock
is reset to 0:00. For that push

9
Start / Stop.
Set time:
To set the time, please return to
actual-value (see page 6) and push
OK for three seconds, then release
the button. The display indicates :00
(= infinite), to activate the hour digit
press Set. Up to 19 hours and 59
min can be reset. The flashing cursor
stands at the ten-minute-digit, the
other digits can be selected with Set
and
Start / Stop.
Change
values:
The selected time digit is increased
or decreased by pushing +or -.
By exceeding the value 9, the next
decade is increased automatically.
By falling below the value 0, the
next-lower decade is selected auto-
matically.
Confirm
process
time:
Push OK and the current actual
value is displayed.
Start
process:
To start a process push
Start / Stop. The preset process
time only starts if the set value is
reached. If a process time is preset,
the horizontal hyphen flashes. After
the expiration of the time, the heat-
ing switches off.

10
Displayed
remaining
time:
The time remaining until the end of
the process is indicated on the dis-
play with flashing “ :“.By pushing
OK for 10 seconds and releasing the
button, you get back to the temper-
ature display.
Operation Shaking
For shaking, the mains switch on the
back of the system must be switched
on.
With the scaled slider, the shaking
frequency can be set to a value
between 0 and 1.500 rpm in steps of 250. For fine-tuning, in steps of 10, use
the keys –and + . The currently entered rotational speed is indicated on the
display for three seconds. The recommended min. rotational speed is 80 rpm,
lower rotational speeds can also be set.
Please note that the HeatingThermo Shaker MHR 11 will shake immediately if
the shaking frequency is set, independent of the start of the tempering.
If the rotational speed is to be displayed during a running process, decrease or
increase the rotational speed with +and – by 10 rotations, the current rota-
tional speed value is then displayed for three seconds.
Assembly of Accessories
Anti-condensation plate BA 24 / 96
The anti-condensation plate BA 96 (800013000) is placed on the attached
block, no assembly steps are required. For using the anti-condensation plate

11
BA 24 (800012900), remove the socket screw in the block with the enclosed
screwdriver. Screw the enclosed threaded pin with isolation knob into the
anti-condensation plate. Place the anti-condensation plate on the block and
screw the threaded pin in deeper so that it takes hold of the bolt of the sys-
tem and with that tightly attaches block and anti-condensation to the system.
Troubleshooting
The display remains blank
Please check if the mains switch on the back side is switched on. If it is
switched on but voltage is present at the socket, check the micro-fuse and
replace if required (IEC 127-2/III, 250 V, 2 A time-lag). This fuse - and a spare
fuse - are located in the IEC-bushing (in which the connection cable is
plugged). The fuse box can be pulled out with a screwdriver.
There are excessive temperature fluctuations
Check the seat of the changeable block by pulling it up. If it is too loose or if
the block exhibits roughness, e.g. contamination, the heat / coolness is not
transferred correctly.
Information on the capacitive touch display
The touch display reacts on finger pressure, even when wearing thin latex
gloves, but not on stylos. Due to its glass surface, the front panel is insensitive
to dirt, chemicals and mechanical damage. Please avoid scratches in the coat-
ing since they can cause malfunctions. Please take note of the following clean-
ing instructions.

12
Maintenance
Cleaning
Regularly clean the housing and the changeable blocks of the
Thermo Shaker and BlockThermostates.
Precautions for avoiding electric shock
Electronic devices can cause electric shocks in case of an operat-
ing error. Never try to repair electric parts. Never open the hous-
ing.
•Switch off the system and disconnect it from the power
supply before starting with cleaning or disinfection
works.
•
Never let get liquids inside the housing (ventilations
slit).
•Do not perform spray disinfection.
•Do only connect the system with the power supply if it
is completely dry.
The repair service may only be performed by staff authorized
and trained by the manufacturer. A modification of the system is
not permitted.
Caution when handling aggressive chemicals
Do not use aggressive chemicals like e.g. strong and weak bases,
strong acids, formaldehyde, acetone, halogenated hydrocarbons
or phenol for cleaning the system and its accessories.
•In case of contamination with aggressive chemicals,
clean the system with a neutral detergent immediately.
•Use neither corrosive detergents nor aggressive sol-
vents or abrasive polishing agents.

13
Cleaning
1. Disconnect the system from the power supply before you start clean-
ing.
2. Clean all outer parts of the system with a mild detergent and a lint-
free cloth.
3. Wipe off the detergent with distilled water.
4. Dry all cleaned parts.
Disinfection
1. Disconnect the system from the power supply before you start disin-
fecting.
2. Let the system cool down.
3. Clean the system as described above.
4. Select a disinfection method compliant to the applicable local legal
regulations and directives.
5. Wipe off all outer parts of the system with the disinfectant and a lint-
free cloth.
Service
If a technical problem arises, please contact your local dealer or the DITABIS
service department. The DITABIS contact information can be found at
www.ditabis.com. If required, the system will be returned for repair – please
take note of our service guidelines, which can be found at www.ditabis.com as
well.
Decontamination before shipment
If you send the system to the authorised technical service for repair or to your
distributor for disposal, decontaminate all parts you want to send. Document
the decontamination in a Decontamination Certificate (incl. serial number)
and include it with the shipment.

14
Technical Data
Technical Data
MHR 11
Temperature-working range
Amb. +3°C to +130°C
Temperature-adjustable range
0°C to +137°C
Accuracy / resolution
+/- 0.1°C / 0.1°C
Max heating time
11.5°C / min.
Shaking frequency
80 – 1,500 rpm
Orbit
3 mm round
Dimensions (without block) W x D x H
220 x 330 x 109 mm
Block - capacity
1 changeable block
Weight (without block)
6.5 kg
Power input
200 W
Electr. supply
230 V, 50 Hz
(115 V / 60 Hz available)
Fuse
2 AT
Protection class
IP21
Environmental conditions
Ambient air temperature
3° – 50°C
in service (non-condensing)
Relative humidity
max. 80%
Technical Data
TH 21
Temperature-working range
Amb. +3°C to +130°C
Temperature-adjustable range
0°C to +137°C
Accuracy / resolution
+/- 0.1°C / 0.1°C
Max heating time
9.5°C / min
Dimensions (without block) W x D x H
220 x 330 x 109 mm
Block - capacity
2 changeable blocks
Weight (without block)
3.5 kg
Power input
200 W
Electr. supply
230 V / 50 Hz

15
(115 V / 60 Hz available)
Fuse
2 AT
Protection class
IP21
Environmental conditions
Ambient air temperature
3° – 50°C
in service (non-condensing)
Relative humidity
max. 80%
Article description blocks & accessories
Blocks
Art. No.
Dimensions of the Containers
For
800010800
BM 02 for 96 x 0,2 ml conical & 8.container stripes
micro
800010900
BM 05 for 38 x 0,5 ml conical
test tubes
800011000
BM 15 for 24 x 1,5 ml conical
800011100
BM 20 for 24 x 2,0 ml cylindrical
For sample
800011500
BP 10 for 24 x D=10,3 mm, 46 mm deep, round bottom, lid
tubes
800011600
BP 12 for 24 x D=12 mm, 20 mm deep, flat bottom
800011700
BP 15 for 24 x D=15 mm, 30 mm deep, flat bottom
800011800
BP 16 for 24 x D=16,5 mm, 46 mm deep, round bottom, lid
800014200
BP 17.0 for 24 x D=17 mm, 30 mm deep, flat bottom
800016100
BP 19.2 for 24 x D=18,7 mm, 25 mm deep, flat bottom
800015200
BP 23.0 for 12 x D=22,5 mm, 56 mm deep, flat bottom
800016000
BP 25.5 for 12 x D=23,0 mm, 56 mm deep, flat bottom
800011900
BP 28 for 8 x D=28 mm, 40 mm deep, flat bottom
For centri-
800012200
BZ 15 "Falcon" tube 14 x 15 ml, with isolation lid
fuge tubes
800012300
BZ 50 for "Falcon" tube 6 x 50 ml, with isolation lid
For PCR
800010400
BC 96 for 96-Well "V" bottom
plates
800010300
BC 84 for 384-Well
For micro
800012000
BV 96 for 96 x round or "V" bottom
plates
800010600
BF 96 for flat bottom
For deep-
well plates
800010500
BD 96 for 96-Deep-Well plates

16
For other
800011200
BN 10 for 36 rectangular cuvettes, outer diameter 12.5 mm
applications
800012100
BW 01 as tub for deep-well, stacked microtiter plates and other
containers, incl. isolation lid and unloading device
800014100
SO 10.4 for 24 x D=10,4 mm, 180 mm deep
800012600
SO 12.0 for 24 x 12ml tubes
800012700
SO 20.5 for 12 x 20ml tubes
For
customized
800010700
BM 00 without boring, height 25 mm
containers
800011300
BO 37 without boring, height 37 mm
800011400
BO 50 without boring, height 50 mm
For slides
800012400
BY 12 for 12 slides for hybridisation
Accessories
Art. No.
Description
800012800
BI 01 Isolation lid
800013800
BT 01 Tempering tub for 1 block, transparent
800013900
BT 02 Tempering tub for 2 blocks, transparent
800012900
BA 24 Anti-condensation plate for 1 block (micro tubes)
800013000
BA 96 Anti-condensation plate for 1 block (PCR/ test plates)
800012500
BH 10 Heating top for the connection with cooling units
800013100
BR 05 Rack with holder f. tubes 0.5 ml
800014300
BR 15 Rack with holder f. tubes 1.5/ 2.0ml

17
Explanations
Caution! Risk of electric shock!
Caution!
Caution! Hot surface!
Important information
Earth conductor
Fuse
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18
Basisdaten
Die HLC by DITABIS Thermomixer und Blockthermostate dienen dem Tempe-
rieren und Mischen von Lösungen in geschlossenen Reaktionsgefäßen und
Platten. Bei der Einstellung der Zieltemperatur der Proben in der Software des
Thermomixers ist darauf zu achten, dass sich diese unterhalb der Siede-
temperatur der zu erwärmenden Lösungen befindet. Im Falle von Unklarhei-
ten im Bezug auf die zu erhitzenden oder zu kühlenden Flüssigkeiten kontak-
tieren Sie bitte DITABIS. Die HLC by DITABIS Geräte sind für die Verwendung
in Innenräumen bestimmt. Verwenden Sie bitte ausschließlich HLC by DITABIS
Zubehör oder das von DITABIS empfohlene Zubehör.
Sicherheit
Die Geräte sind konform zu den in der zugehörigen CE-Erklärung genannten
Normen und Richtlinien.
Bitte beachten Sie folgende Sicherheitsmaßnahmen:
Schließen Sie das Gerät nur an eine Schuko-Steckdose 230 V,
50 Hz an.
Stellen Sie keine heißen Blöcke auf eine brenn- oder
schmelzbare Unterlage.
Stellen Sie sicher, dass die verwendeten Gefäße im Block für
den gewünschten Temperaturbereich geeignet sind.
Sollte einmal Flüssigkeit in das Gerät gelangen, ziehen Sie
den Netzstecker und kontaktieren Sie unsere Serviceabtei-
lung, damit vollständige Sicherheit gewährleistet ist.
Bitte berühren oder transportieren Sie kein heißes Gerät.
Vermeiden Sie jeden Kontakt des Gerätes mit leicht entzünd-
lichen Flüssigkeiten.
Die Lüftungsschlitze des Gerätes müssen jederzeit frei zu-
gänglich sein.
Defekte Netzkabel dürfen ausschließlich durch gleichwertige
Netzkabel ersetzt werden.

19
Gewährleistung und Garantie
Alle Funktionen der Systeme wurden ausführlich getestet. Das Gerät und das
Zubehör wurden anschließend sorgfältig verpackt.
Wenn dennoch beim Aufstellen oder der Inbetriebnahme Schäden oder Män-
gel festgestellt werden sollten, ist dies bitte umgehend DITABIS mitzuteilen
(siehe AGB auf www.ditabis.com). Bei Benutzung unter normalen Laborbedin-
gungen und unter Beachtung der Bedienungsanleitung beträgt die Gewähr-
leistung ein Jahr auf Material und Verarbeitung, gerechnet vom Tage des
Versandes.
Als Premium-Kunde erweitert sich die Garantie auf insgesamt drei Jahre und
Sie erhalten eine kostenlose Reparatur im Garantiefall - auch bei Verschleiß-
teilen. Des Weiteren erhalten Sie ein kostenloses Ersatzgerät innerhalb von
48h im Reparaturfall. Unser kostenloser Rückrufservice und E-Mailsupport mit
einer Reaktionszeit von 24h steht Ihnen ebenso zur Verfügung wie unser
kostenloser Abhol- und Rücksendungsservice.
Die Premium-Kunden-Garantie wird mit den oben beschriebenen Konditionen
in Deutschland angeboten. Für andere Länder gelten andere Angebote, die
wir Ihnen gerne zukommen lassen.
Haftungsausschluss
Bei nicht sachgemäßem Gebrauch der Geräte übernimmt der Hersteller keine
Haftung.
Wichtige Hinweise:
Diese Bedienungsanleitung wurde auf Grundlage der EN DIN 61010-1 Norm
erstellt. IQ, OQ und PQ Dokumente erhalten Sie gesondert auf Anfrage.
Die Geräte sind mit einem hochgenauen, selbstoptimierenden
Temperaturregler ausgestattet. Es ist durchaus normal, dass im
Rahmen der Selbstoptimierung im Einzelfall die eingestellte Tem-
peratur leicht überschritten wird.
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20
Es ist darauf zu achten, dass sich bei der Erhitzung von Proben ge-
fährliche Gase bilden können. Sollte dies der Fall sein, müssen die
Geräte in Abzügen verwendet werden.
Bei hoher Schüttelfrequenz der Thermomixer ist es möglich, dass
sich die Vibrationen des Gerätes auf die Stellfläche übertragen.
Bitte berücksichtigen Sie dies bei der Platzierung des Gerätes.
Vermeiden Sie jeden Kontakt des Gerätes mit leicht entzündlichen
Flüssigkeiten, da der Block / die Blöcke sehr heiß werden können.
Die Lüftungsschlitze des Gerätes müssen jederzeit frei zugänglich
sein. Ein Mindestabstand von 10 cm an den Seiten des Gerätes
muss eingehalten werden, damit eine korrekte Belüftung gewähr-
leistet ist.
Umgebungsbedingungen
Der optimale Funktionsbereich der Geräte liegt bei einer relativen Luftfeuch-
tigkeit von max. 80% und einer Umgebungstemperatur zwischen +3°C bis
max. +50°C. Diese Werte sollten nicht über- oder unterschritten werden.
Temperaturgenauigkeit
Jedes HLC by DITABIS-Gerät wird mit einem geeichten, hochgenauen Tempe-
raturmessgerät auf Temperatur-Genauigkeit und -Reproduzierbarkeit kalib-
riert. Dieser Prozess findet bei einer Raumtemperatur von +20°C und einer
Blocktemperatur von +37°C statt. Die Absolutkalibrierung der Heizeinheit
kann auch kundenspezifisch für einen bestimmten Temperaturbereich durch-
geführt werden. HLC by DITABIS erreicht eine sehr hohe Genauigkeit u.a.
dadurch, dass die Blocktemperatur mitten im Block gemessen wird. Es kann
jedoch vorkommen, dass bei großen Temperaturdifferenzen zwischen Raum-
und Blocktemperatur ein entsprechender Einfluss der kalten Raumtemperatur
auf die Probentemperatur wirkt. Um diesen Einfluss zu reduzieren, empfehlen
wir, die Antikondensplatte BA 24 / BA 96 oder die Temperierhaube BT 01 / BT
02 zu verwenden, wenn eine hohe Temperaturgenauigkeit in der Probe er-
reicht werden soll. Bitte beachten Sie bei der Vorgabe einer Prozesszeit, dass