Home Science Tools MI-1000STD User manual

Instruction Manual for
MI-1000STD – Kids Microscope
MI-1000LED – Kids LED Cordless Microscope
1. 800.860.6272
www.homesciencetools.com
Copyright 2006 by Home Training Tools, Ltd.
All rights reserved.
Kids Microscope

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Welcome to an exciting world of
discovery with your new Kids Microscope!
This manual will give you a familiarity with the
different features of your microscope, how to
use them, and how to preserve your investment
by proper maintenance and care.
There are two microscopes in the Kids
Microscope series. They share the same basic
features and functions, but you will find a
discussion of the power options for the MI-
1000LED model on page 3.
Table of Contents
General Microscope Care .................................... 2
Unpacking......................................................... 2
Cleaning ........................................................... 2
Features & Definitions.......................................... 2
Microscope Diagram......................................... 2
Description of Components .............................. 2
Power Options for MI-1000LED ....................... 3
Operating Procedure............................................ 3
Maintenance......................................................... 4
Adjusting the Stage Stop.................................. 4
Changing the Tungsten Bulb in the MI-1000STD ...... 4
Changing the LED Bulb in the MI-LEDMIC ...... 4
Troubleshooting ................................................... 5
Specifications ....................................................... 5
Ideas for Using Your Microscope......................... 6
Microscope Observation
Worksheet……………...7
Warranty............................................................... 8
General Microscope Care
Unpacking
Your Kids Microscope is shipped in a two-
part Styrofoam case. Keep this case for storage,
transport, and shipping. It is perfect packing
material should you ever need to send your
microscope in for repairs covered by the
warranty.
When handling your microscope, always
pick it up by the arm. Avoid touching the lens
surfaces on the eyepiece or objective lens, as
finger prints will decrease image quality.
Cleaning
The best optical quality can be
compromised by dirty lenses. Using a dustcover
and cleaning the lenses regularly will greatly
enhance your microscope use.
To clean lens surfaces, remove dust by
using a soft brush or a can of compressed air.
Then moisten a piece of lens paper (our item MI-
PAPER) with some lens cleaning solution (MI-
LENSCLN). Gently clean the eyepiece and
objective lens exterior surface using a circular
motion. Repeat with a second paper moistened
with solution if necessary. Repeat once again
with a piece of dry lens paper until the lens is
clean and dry. Do not spray lens cleaner
directly on the lens.
Features & Definitions
Microscope Diagram
Description of Components
1. Eyepiece: This is the part of the microscope
that you look through. It is inclined at a 45º
angle for comfortable viewing. It contains a
lens that magnifies 10x.
2. Arm: The arm not only supports the head
and nosepiece, it is also the best “handle”
for picking up and moving the microscope.
3. Nosepiece: This is also called the “objective
turret.” It holds the objective lenses and
rotates 360º. You can change magnification
by turning it until the lens you want to use
“clicks” into place.
4. Objective lenses: These are the lenses
1. Eyepiece
2. Arm
3. Nosepiece
4. Objective
lenses
5. Stage
6. Stage stop
7. Stage clips
8. Disc diaphragm 9. Focus knob
10. Illuminator

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closest to the specimen. The standard
objectives are 4x, 10x, and 40x, which
multiply with the 10x eyepiece lens to
provide magnification levels of 40x, 100x,
and 400x. The shortest lens has the lowest
magnification level, while the longest has the
highest. The lenses have the following
features:
•They are achromatic – they help
prevent color distortion.
•They are parcentered – if you center
your slide using one objective, it will
still be centered when you move to
another objective.
•They are parfocal – if you focus your
specimen using one objective, it will
stay coarsely focused when you
move to another objective. (You will
still have to make minor
adjustments.)
•The 40x objective is retractable –
the tip containing the lens is spring-
loaded to prevent damage to the
objective or slide.
5. Stage: The stage is the platform that
supports the specimen slide below the
objective lenses. It moves up and down
when you turn the focus knob, allowing you
to get just the right distance between the
slide and the lens.
6. Stage stop: This is a screw with a lock nut
located between the stage and the arm of
the microscope. It prevents the stage from
coming too far up and grinding against the
objective lens. It is also called a “safety rack
stop,” and is pre-adjusted by the
manufacturer. Instructions for readjusting it
manually are on page 4.
7. Stage clips: The stage clips hold
microscope slides in place. Pressing on the
end closest to the arm of the scope will lift
up the other end, allowing you to place your
slide underneath.
8. Disc diaphragm: The diaphragm controls
the amount of light coming through the
specimen in order to provide optimum
resolution for the objective lens. The
diaphragm on this microscope is a rotating
disc under the stage with holes that are
numbered by size; for example, a hole
labeled 6 is 6mm in diameter and a hole
labeled 2 has a diameter of 2mm. Use the
smaller holes for lower magnification and the
larger holes for higher magnification.
9. Focus knob: The focus knob is used to
raise or lower the stage until the image is in
focus. The focus mechanism uses a slip
clutch to prevent damage to the gears.
10. Illuminator: The illuminator provides light
underneath the stage. The MI-1000STD
contains a 15-watt tungsten bulb. The MI-
1000LED contains an LED bulb and light
intensity control knob located on the base.
This intensity control helps adjust illumination
contrast. Instructions for changing the bulbs
are on page 4.
Power Options for MI-1000LED
The LED Microscope comes with a built-in
rechargeable NiMH battery and charger. The
fully charged battery provides about 24 hours of
totally portable microscope use. The AC adapter
is used to recharge the battery. (The battery
should be fully charged before first use, or use
the adapter.)
Red and green lights on the back of the
microscope indicate charging status. Please
follow these charging guidelines to maintain
maximum battery life for your microscope.
1. Turn off the illuminator and plug in the AC
adapter.
2. A red light only indicates the battery is
charging and has less than 70% of full
charge.
3. Both a red and green light indicates the
battery is charging and has 70-90% of
charge.
4. A green light only indicates the battery is
fully charged and ready for use.
5. Typical charging time is 4-8 hours. Do not
charge the battery or leave the AC adapter
plugged in for more than 12 hours.
Operating Procedure
Now that you have an overview of what
each component of your microscope is for, you
can follow this step-by-step procedure to help
you get started using it.
1. Set your microscope on a table or other flat
surface where you will have plenty of room
to work. Plug the microscope’s power cord
into an outlet, making sure that the excess
cord is out of the way so no one can trip
over it. (The MI-1000LED also operates on
battery power.)

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Sto
p
screw
Locking nut
2. Flip the switch to turn on your microscope's
light source and then turn the disc
diaphragm to the largest hole, which allows
the greatest amount of light through. (You
will adjust this again later for best contrast.)
The MI-1000LED also has a light intensity
control on the base: turn the intensity up
fully.
3. Rotate the nosepiece to the lowest-power
(4x) objective. You will hear a click when it is
properly in place. Always start with the
lowest power: it is easiest to scan a slide at
a low setting, as you have a larger field of
view.
4. Turn the focus knob to move the stage down
(away) from the objective lens as far as
possible.
5. Set a microscope slide (coverslip facing up)
in place under the stage clips. A prepared
slide works best when you do this for the
first time. Move the slide until the specimen
is under the objective lens.
6. Adjust the focus knob until the specimen is
in focus. Slowly move the slide to center the
specimen under the lens, if necessary, by
nudging it with your fingers.
7. Adjust the diaphragm to get the best lighting.
Start with the most light and gradually
lessen it until the specimen image has clear,
sharp contrast. On the MI-1000LED you can
also adjust the light intensity control for
contrast.
8. Scan the slide (right to left and top to
bottom) at low power to get an overview of
the specimen (nudge the slide very slowly
with your fingers). Then center the part of
the specimen you want to view at higher
power.
9. Rotate the nosepiece to the 10x for 100x
magnification. Refocus and view the slide
carefully. Adjust the diaphragm again until
the image has the best contrast. Repeat with
the 40x objective for 400x magnification.
Maintenance
Adjusting the Stage Stop
The stage stop is set at the factory to
ensure that the stage cannot come up far
enough to hit the objective lenses. Under normal
circumstances you will not have to adjust this.
However, if you cannot focus a slide, follow
these steps:
1. Loosen the knurled locking
nut by turning it counter-
clockwise. (Use needle-
nose pliers for this.)
2. Loosen the stop screw.
3. Focus on a standard slide
until you obtain a sharp image.
4. Tighten the stop screw by turning it
clockwise until it stops, then turn it back ½
turn.
5. Re-tighten the locking nut.
Changing the Tungsten Bulb in the MI-
1000STD
1. Obtain the correct 15-watt tungsten
replacement bulb (our item MI-BULB2). One
is included with your microscope.
2. Unplug your microscope from the power
supply and allow it to cool before replacing
the bulb.
3. Carefully lay the microscope on its side.
4. Using a screwdriver, remove the
screw from the center of each
rubber foot.
5. Remove the bottom plate and
gently push the bulb in and turn
it to release it from the socket.
6. Replace with a new bulb, then
put the plate back in place and
replace the rubber feet.
Changing the LED Bulb in the MI-
LEDMIC
1. Obtain the correct LED replacement bulb
(our item MI-BULB10). One is included with
your microscope.
2. Unplug the microscope from the power
supply and allow it to cool before replacing
the bulb.
3. Carefully lay the microscope on its side.
4. Using a screwdriver, remove the screw from
the center of each rubber foot.
5. Remove the bottom plate
and pull the used LED
bulb straight out from its
socket.
6. Replace with a new LED
bulb and then screw the plate back on.

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Troubleshooting
If you are experiencing difficulty with your microscope, try these troubleshooting techniques:
Problem Possible Reason and Solution
Light fails to
operate
1. The batteries are dead (MI-1000LED). Use the AC adapter to recharge the batteries.
2. The light intensity control is off (MI-1000LED). Turn up the light intensity.
3. The bulb is burned out. Replace the bulb. (See “Changing the Bulb,” p. 4.)
4. The incorrect bulb is installed. Replace with the correct bulb.
Light flickers 1. The bulb is not properly inserted into the socket. Properly insert the bulb.
2. The bulb is about to burn out. Replace the bulb.
No image 1. The nosepiece is not indexed properly. Move revolving nosepiece until the objective
lens clicks into position.
2. The light is too bright. Adjust the diaphragm.
Unable to
focus slide
1. The slide coverslip is too thick. Use 0.17 mm thick (No. 1) coverslip.
2. The slide is upside down. Place the slide on the stage with the coverslip facing up.
3. The stage stop is not set at the proper position. Adjust the stage stop. (See
“Adjusting the Stage Stop,” p. 4.)
Poor
resolution,
image not
sharp
1. The objective or eyepiece lenses are dirty. Clean the lenses. (See “Cleaning,” p. 2.)
2. There is too much light. Adjust the diaphragm.
Spots in field 1. The specimen slide, objective, or eyepiece lens is dirty. Clean the slide or lenses.
(See “Cleaning,” p. 2.)
Uneven
illumination of
field
1. The nosepiece is not indexed properly. Move revolving nosepiece until the objective
lens clicks into position.
2. The diaphragm is not properly indexed. Adjust the diaphragm to the proper level.
Specifications
Eyepiece Widefield 10x eyepiece with fully coated optics. Inclined 45° head.
Nosepiece 3-hole, ball-bearing mounted with positive click stops.
Objectives All objectives are achromatic, parfocalled, parcentered, and fully coated.
•4x, 0.10 N.A., red ring, 4.0mm field of view, 40x magnification
•10x, 0.25 N.A., yellow ring, 1.6mm field of view, 100x magnification
•40x, 0.65 N.A., blue ring, .40mm field of view, 400x magnification, retractable
Focusing Single intermediate focusing control with slip clutch. All metal rack-and-pinion focusing with
adjustable stage stop.
Stage Acid and chemical resistant 95 x 95mm metal stage with stage clips (not designed for use
with a mechanical stage).
Condenser Fixed 0.65 NA condenser.
Diaphragm Calibrated 6-hole disc diaphragm.
Illuminator 15-watt tungsten illuminator with grounded 110-volt cord on model MI-1000STD, 20-watt
equivalent LED illuminator with AC Adapter or optional batteries on model MI-1000LED.

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Ideas for Using Your Microscope
You have a microscope—now what? With
the following directions you can get started right
away making your own microscope slides!
How to Make Simple Microscope Slides
Learn more about using the Kids
microscope by making simple slides using
common items from around the house!
Materials Needed:
-clear Scotch tape
-a few granules of salt, sugar, ground
coffee, sand, or any other grainy
material
Making Simple Slides
To make a slide, tear a 2½-3” long piece of
Scotch tape and set it sticky side up on the
kitchen table or other work area. Fold over about
½” of the tape on each end to form finger holds
on the sides of the slide. Next, sprinkle a few
grains of salt or sugar in the middle of the sticky
part of the slide.
You can repeat this with the other
substances if you like, just be sure to label each
slide you make with an ink pen or permanent
marker so you will know what’s on the slides!
You can make tape slides with many other
materials as well. Try hair (from pets and family
members), thread and fiber (from carpets or
clothing), or small dead insects such as gnats,
ants, or fruit flies. Label each slide and view
them one at a time with your microscope,
experimenting with different magnification.
How to Make Your Own Prepared Slide
Learn how to make temporary mounts of
specimens and view them with your microscope.
Below are a few ideas for studying different
types of cells found in items that you probably
already have around your house.
Cork Cells
In the late 1600s, a scientist named Robert
Hooke looked through his microscope at a thin
slice of cork. He noticed that the dead wood was
made up of many tiny compartments, and upon
further observation Hooke named these empty
compartments cells. It was later known that the
cells in cork are only empty because the living
matter that once occupied them has died and
left behind tiny pockets of air. You can take a
closer look at the cells, also called lenticels, of a
piece of cork by following these instructions.
Materials Needed:
-small cork
-plain glass microscope slide
-slide coverslip
-sharp knife or razor blade
-water
How to make the microscope slide:
Carefully cut a very thin slice of cork using
a razor blade or
sharp knife (the
thinner the slice, the
easier it will be to
view with your
microscope). To
make a wet mount of the cork, put one drop of
water in the center of a plain glass slide – the
water droplet should be larger than the slice of
cork. Gently set the slice of cork on top of the
drop of water (tweezers might be helpful for
this). If you are not able to cut a thin enough
slice of the whole diameter of the cork, a smaller
section will work.
Take one coverslip and hold it at an angle
to the slide so that one
edge of it touches the
water droplet on the
surface of the slide.
Then, being careful not to move the cork
around, lower the cover slip without trapping any
air bubbles beneath it. The water should form a
seal around the cork. Use the corner of a paper
towel to blot up any excess water at the edges
of the coverslip. To keep the slide from drying
out, you can make a seal of petroleum jelly
around the cover slip with a toothpick. Begin
with the lowest-power objective to view your
slide. Then switch to a higher power objective to
see more detail. Use this same wet mount
method for other specimens such as cheek cells
or leaf cells.
Record Your Observations
Our Microscope Observation worksheet (on
the next page) will help you keep track of what
you see and remember what you have learned.
Blanks are provided for recording general
information about each slide (e.g. wet mount
stained with methylene blue). In addition, there
is space to write down your observations and
make sketches of what you see at each
magnification level.

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Date of slide:
Name of sample:
Collected from:
Stain:
Mount:
Lighting:
Observations
Sketches
40x magnification
400x magnification Other: _____________
100x magnification

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Warranty
Home Science Tools warrants this microscope to be free from defects in material and workmanship under
normal use and service for the life of the instrument. This warranty does not cover light bulbs, batteries,
or damage due to misuse, abuse, alterations, or accident. Warranty does not cover lenses that have
become inoperable due to excessive dirtiness as a result of misuse or lack of normal maintenance.
Any cameras and software supplied with this microscope are warranted for one year from the date of
purchase.
You will need to return your microscope freight prepaid for warranty service to Home Science Tools, or
the repair facility we designate. We will repair or replace your microscope at no charge and return it
freight prepaid to you. Please call 1-800-860-6272 to arrange warranty service before returning this
instrument.
Please note that warranties apply only to the original purchaser and are not transferable.
This manual suits for next models
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