HYDROLAB HL Series User manual

DOC026.97.80349
HL Series Sensors
09/2019, Edition 4
User Manual
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Table of Contents
English..............................................................................................................................3
Deutsch.......................................................................................................................... 46
Italiano............................................................................................................................ 91
Français....................................................................................................................... 135
Español........................................................................................................................ 180
Português.................................................................................................................... 225
中文............................................................................................................................... 269
日本語........................................................................................................................... 310
Polski............................................................................................................................ 354
2

Table of Contents
1 Safety information on page 3
2 Ammonium/Ammonia sensor (007255)
on page 5
3 Blue-green algae freshwater sensors (007293,
007291) on page 8
4 Blue-green algae marine sensors (007299,
007301, 9061000) on page 11
5 Central cleaning brush, HL7 (9195800)
on page 13
6 Chloride sensor (007254) on page 15
7 Chlorophyll a sensors (007284, 007202, 9060600)
on page 18
8 Conductivity sensor (004468) on page 21
9 Depth sensors on page 23
10 Nitrate sensor (007256) on page 24
11 Hach® LDO sensor (9152000) on page 27
12 pH sensors (007234, 007264) on page 30
13 pH/ORP sensors (007233, 007235) on page 32
14 Reference sensor (004463) on page 35
15 Rhodamine WT sensors (007285, 007204,
9060800) on page 36
16 Temperature sensor (004165HY) on page 39
17 Turbidity sensor, HL4 (9195200) on page 40
18 Turbidity sensor with cleaning brush, HL7
(9195600) on page 43
Section 1 Safety information
N O T I C E
The manufacturer is not responsible for any damages due to misapplication or misuse of this product including,
without limitation, direct, incidental and consequential damages, and disclaims such damages to the full extent
permitted under applicable law. The user is solely responsible to identify critical application risks and install
appropriate mechanisms to protect processes during a possible equipment malfunction.
Please read this entire manual before unpacking, setting up or operating this equipment. Pay
attention to all danger and caution statements. Failure to do so could result in serious injury to the
operator or damage to the equipment.
Make sure that the protection provided by this equipment is not impaired. Do not use or install this
equipment in any manner other than that specified in this manual.
D A N G E R
Chemical or biological hazards. If this instrument is used to monitor a treatment process and/or
chemical feed system for which there are regulatory limits and monitoring requirements related to
public health, public safety, food or beverage manufacture or processing, it is the responsibility of the
user of this instrument to know and abide by any applicable regulation and to have sufficient and
appropriate mechanisms in place for compliance with applicable regulations in the event of malfunction
of the instrument.
1.1 Use of hazard information
D A N G E R
Indicates a potentially or imminently hazardous situation which, if not avoided, will result in death or serious injury.
WARNING
Indicates a potentially or imminently hazardous situation which, if not avoided, could result in death or serious
injury.
CAUTION
Indicates a potentially hazardous situation that may result in minor or moderate injury.
N O T I C E
Indicates a situation which, if not avoided, may cause damage to the instrument. Information that requires special
emphasis.
English 3

1.2 Precautionary labels
Read all labels and tags attached to the instrument. Personal injury or damage to the instrument
could occur if not observed. A symbol on the instrument is referenced in the manual with a
precautionary statement.
Electrical equipment marked with this symbol may not be disposed of in European domestic or public
disposal systems. Return old or end-of-life equipment to the manufacturer for disposal at no charge to
the user.
1.3 Certification
CAUTION
This equipment is not intended for use in residential environments and may not provide adequate protection to
radio reception in such environments.
Canadian Radio Interference-Causing Equipment Regulation, IECS-003, Class A:
Supporting test records reside with the manufacturer.
This Class A digital apparatus meets all requirements of the Canadian Interference-Causing
Equipment Regulations.
Cet appareil numérique de classe A répond à toutes les exigences de la réglementation canadienne
sur les équipements provoquant des interférences.
FCC Part 15, Class "A" Limits
Supporting test records reside with the manufacturer. The device complies with Part 15 of the FCC
Rules. Operation is subject to the following conditions:
1. The equipment may not cause harmful interference.
2. The equipment must accept any interference received, including interference that may cause
undesired operation.
Changes or modifications to this equipment not expressly approved by the party responsible for
compliance could void the user's authority to operate the equipment. This equipment has been tested
and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC rules.
These limits are designed to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment generates, uses and can
radiate radio frequency energy and, if not installed and used in accordance with the instruction
manual, may cause harmful interference to radio communications. Operation of this equipment in a
residential area is likely to cause harmful interference, in which case the user will be required to
correct the interference at their expense. The following techniques can be used to reduce
interference problems:
1. Disconnect the equipment from its power source to verify that it is or is not the source of the
interference.
2. If the equipment is connected to the same outlet as the device experiencing interference, connect
the equipment to a different outlet.
3. Move the equipment away from the device receiving the interference.
4. Reposition the receiving antenna for the device receiving the interference.
5. Try combinations of the above.
4 English

Section 2 Ammonium/Ammonia sensor (007255)
2.1 Specifications
Specifications are subject to change without notice.
Specification Details
Range 0 to 250 mg/L-N
Accuracy ± 10% or ± 2 mg/L-N (the larger value)
Resolution 0.01 mg/L-N
Parameters NH4+, NH3Tot (total ammonia nitrate), NH3 or sensor mV
Interferences Sodium interferes with measurements at 1000 µS/cm and higher
Deployment life of sensor cartridge 3 to 4 months
Life1 of sensor cartridge 6 months
Maximum depth 15 m (49 ft)
Operating temperature 0 to 40 °C (32 to 104 °F)
2.2 Product overview
The ammonium/ammonia sensor (007255) is gray. Refer to Figure 1.
There is a replaceable sensor cartridge installed in the sensor. Refer to Specifications on page 5 for
the deployment life of the sensor cartridge.
For best results, use the sensor in waters that are pH 4 to 10. Refer to Specifications on page 5 for
measurement interferences.
The sensor cartridge has a depth limit of 15 meters (49 feet). Remove the sensor cartridge and install
the sensor cap before the sonde is deployed deeper than 15 m (49 ft) to prevent damage to the
sensor cartridge. Refer to the illustrated steps in Figure 2.
To extend the life of the sensor cartridge, remove the sensor cartridge and install the sensor cap
when the sensor is not in use. Refer to the illustrated steps in Figure 2.
Note: At pH levels of 6 or less, water contains ammonium (NH4+) and no ammonia. At pH 12 and higher, water
contains ammonia (NH3) and no ammonium. Between pH 6 and pH 12, water contains a mix of ammonium and
ammonia.
Figure 1 Nitrate sensor
1 Sensor 3 Sensor cap
2 Sensor cartridge 4 Glass vial for sensor cartridge storage
1Deployment life plus the storage life
English 5

Figure 2 Sensor cartridge removal
2.3 Calibration
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
Calibrate the sensors before initial use. At regular intervals, do a calibration check to make sure that
measurements stay within the tolerance range. Calibrate the sensors if measurements are out of the
tolerance range. The deionized (DI) water steps must be done for an accurate calibration.
Refer to the Hydrolab Operating Software Manual for instructions on how to do the tasks that follow:
• Start a sensor calibration.
• Start a sensor calibration check.
• Set the calibration mode.
• Select the measurement units shown during calibration and calibration checks.
• Look at the calibration history.
To look at the Hydrolab Operating Software Manual, start the Hydrolab Operating Software, click
Help and select Hydrolab Operating Software Help.
When a calibration or a calibration check is started, the step-by-step instructions for the sensor show
on the display.
2.4 Sensor guidelines
Keep deposits off the sensor for best measurements. Examine the sensor to make sure that there
are no contaminants. Keep the substances shown in Table 1 away from the sensor.
Table 1 Contaminants
Substance Damage
Oil, sediment or biofilms Contaminates the sensor. If the sensor is contaminated, clean the sensor.
6 English

2.5 Maintenance
2.5.1 Clean the sensor
N O T I C E
To prevent damage to the sensor, do not use organic solvent solutions (e.g., acetone or methanol) to clean the
sensor. Organic solvents cause damage to the plastic housing and sensor membrane.
N O T I C E
To prevent damage to the sensor membrane, do not use cleaning chemicals to clean the sensor membrane.
Clean the sensor after each deployment to remove unwanted material (e.g., oil, biological growth and
dirt). In addition, clean the sensor before and after calibration.
Adjust the sonde deployment time in accordance with how quickly sensor fouling occurs in the
deployment waters.
1. Clean the sensor with:
• Mild detergent
• Warm water
• Clean, soft toothbrush
Note: Do not use dishwashing liquid to clean the sensor. Dishwashing liquid and household cleaners with skin
moisturizer can put a film on the sensor.
2. Rinse the sensor with clean water.
3. Soak the sensor in clean water for a minimum of 30 minutes.
4. Examine the sensor cartridge membrane. If unwanted material is seen on the sensor cartridge
membrane, use only a soft brush to carefully sweep the sensor cartridge membrane or damage
can occur.
5. Rinse the sensor with clean water.
6. If the substance cannot be removed, contact technical support.
2.5.2 Replace the sensor cartridge
Replace the sensor cartridge when it can no longer be calibrated or if the sensor cartridge is
damaged. Refer to the illustrated steps that follow.
English 7

2.6 Consumables and replacement parts
Consumables
Description Quantity Item no.
Low NH4+ Ammonium Calibration Solution, 4.62 mg/L-N 500 mL 002587HY
High NH4+ Ammonium Solution, 46.2 mg/L-N 500 mL 002588HY
Replacement parts
Description Item no.
Ammonium/Ammonia sensor cartridge, gray 003522
Sensor cap 003960
Section 3 Blue-green algae freshwater sensors (007293, 007291)
3.1 Specifications
Specifications are subject to change without notice.
Specification Details
Range 0 to 40,000 ppb
Linearity R2 = 0.999
MDL ppb 0.062
MDL RFU 0.0002
Parameters µg/L, ppb, volts, RFU
Note: ppb is equivilent to ug/L. RFU is 0–100 based on the full detection range of the sensor.
Maximum depth 200 m (656 ft)
Operating temperature –5 to 50 °C (23 to 122 °F), non-freezing
3.2 Product overview
N O T I C E
Do not put the sensor in red pH 4 buffer. Red pH 4 buffer can cause permanent damage to the sensor lenses. As
an alternative, use pH 4 buffer with no color.
The blue-green algae freshwater sensor is an optical fluorometer that determines the concentration
of blue-green algae in freshwater. Refer to Figure 3.
This sensor is sensitive to light. For the best results, point the end of the sensor away from light
sources.
Figure 3 Blue-green algae freshwater sensor
2Phycocyanin pigment from Prozyme diluted in deionized water
8 English

3.3 Theory of operation
The sensor irradiates the freshwater with orange light (590 nm). The blue-green algae in the water
absorbs the orange light energy and gives red light (650 nm). The sensor directly measures the
quantity of red light in the water to determine the concentration of blue-green algae. The reading is
0 to 40,000 ppb (or 0 to 5 V).
3.4 Calibration
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
Calibrate the sensors before initial use, at regular intervals and after sensor maintenance or
modifications. Periodically, do a calibration check to make sure that measurements are still within
tolerance range.
Refer to the Hydrolab Operating Software Manual for instructions on how to do the tasks that follow:
• Start a sensor calibration.
• Start a sensor calibration check.
• Set the calibration mode.
• Select the measurement units shown during calibration and calibration checks.
• Look at the calibration history.
To look at the Hydrolab Operating Software Manual, start the Hydrolab Operating Software, click
Help and select Hydrolab Operating Software Help.
When a calibration or a calibration check is started, the step-by-step instructions for the sensor show
on the display.
3.4.1 Use a solid secondary standard
Use a solid secondary standard to verify the calibration of the sensor when a calibration check is
done. Refer to Accessories for ordering information.
Use a solid secondary standard only to verify the calibration of the sensor on which the value is set.
Set the value of the solid secondary standard as follows:
1. Make sure that the optical surface of the sensor is clean and dry.
2. Put the sensor into a sample with a known concentration or a Rhodamine Calibration Dye
Solution.
3. Record the sensor output voltage (0 to 5 V).
4. Clean and dry the sensor.
5. Put the solid secondary standard on the optical end of the sensor.
6. Turn the solid secondary standard until it is aligned with the indexing mark on the sensor. A
clicking sound is heard when the solid secondary standard is correctly aligned.
7. Use the supplied screwdriver to turn the screw located on the side of the solid secondary
standard. Turn the screw until the sensor output voltage is the same as the value recorded.
Note: Turn the screw clockwise to increase the voltage. Turn the screw counter-clockwise to decrease the
voltage.
English 9

3.5 Sensor guidelines
Keep deposits off the sensor for best measurements. Examine the sensor to make sure that there
are no contaminants. Keep the substances shown in Table 2 away from the sensor.
Table 2 Contaminants
Substance Damage
Oil, sediment or biofilms Contaminates the sensor. If the sensor is contaminated, clean the sensor.
3.6 Maintenance
3.6.1 Clean the sensor
N O T I C E
To prevent damage to the sensor, do not use organic solvent solutions (e.g., acetone or methanol) to clean the
sensor. Organic solvents cause damage to the plastic housing and sensor lenses.
Clean the sensor after each deployment to remove unwanted material (e.g., oil, biological growth and
dirt). In addition, clean the sensor before and after calibration.
Adjust the sonde deployment time in accordance with how quickly sensor fouling occurs in the
deployment waters.
1. Clean the sensor with:
• Mild detergent
• Warm water
• Clean, soft toothbrush
• Soft cloth and/or cotton swabs
Note: Do not use dishwashing liquid to clean the sensor. Dishwashing liquid and household cleaners with skin
moisturizer can put a film on the sensor.
2. Rinse the sensor with clean water.
3. Soak the sensor in clean water for a minimum of 30 minutes.
4. Examine the optical windows. If unwanted material is seen on the optical windows, use optical
tissue or a cotton swab with soap and water to clean the optical windows.
5. Rinse the sensor with clean water.
6. If the substance cannot be removed, contact technical support.
3.7 Accessories
WARNING
Personal injury hazard. Use of non-approved parts may cause personal injury, damage to the
instrument or equipment malfunction. The replacement parts in this section are approved by the
manufacturer.
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to
the company website for contact information.
Description Quantity Item no.
Rhodamine Calibration Dye Solution, 21.33 ± 2.5 w/w% 236 mL (8 oz) 007273
Solid Secondary Standard 1 007726
10 English

Section 4 Blue-green algae marine sensors (007299, 007301,
9061000)
4.1 Specifications
Specifications are subject to change without notice.
Specification Details
Range 0 to 750 ppb
Linearity R2 = 0.9999
MDL ppb 0.083
MDL RFU 0.00026
Parameters µg/L, ppb, volts, RFU
Note: ppb is equivilent to ug/L. RFU is 0–100 based on the full detection range of the sensor.
Maximum depth 200 m (656 ft)
Operating temperature –5 to 50 °C (23 to 122 °F), non-freezing
4.2 Product overview
N O T I C E
Do not put the sensor in red pH 4 buffer. Red pH 4 buffer can cause permanent damage to the sensor lenses. As
an alternative, use pH 4 buffer with no color.
The blue-green algae marine sensor is an optical fluorometer that determines the concentration of
blue-green algae in saltwater. Refer to Figure 4.
This sensor is sensitive to light. For the best results, point the end of the sensor away from light
sources.
Figure 4 Blue-green algae marine sensor
4.3 Theory of operation
The sensor irradiates the saltwater with green light (530 nm). The blue-green algae in the water
absorbs the green light energy and gives orange light (570 nm). The sensor directly measures the
quantity of orange light in the water to determine the concentration of blue-green algae. The reading
is 0 to 750 ppb (or 0 to 5 V).
4.4 Calibration
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
3Phycoerythrin pigment from Prozyme diluted in deionized water
English 11

CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
Calibrate the sensors before initial use, at regular intervals and after sensor maintenance or
modifications. Periodically, do a calibration check to make sure that measurements are still within
tolerance range.
Refer to the Hydrolab Operating Software Manual for instructions on how to do the tasks that follow:
• Start a sensor calibration.
• Start a sensor calibration check.
• Set the calibration mode.
• Select the measurement units shown during calibration and calibration checks.
• Look at the calibration history.
To look at the Hydrolab Operating Software Manual, start the Hydrolab Operating Software, click
Help and select Hydrolab Operating Software Help.
When a calibration or a calibration check is started, the step-by-step instructions for the sensor show
on the display.
4.4.1 Use a solid secondary standard
Use a solid secondary standard to verify the calibration of the sensor when a calibration check is
done. Refer to Accessories for ordering information.
Use a solid secondary standard only to verify the calibration of the sensor on which the value is set.
Set the value of the solid secondary standard as follows:
1. Make sure that the optical surface of the sensor is clean and dry.
2. Put the sensor into a sample with a known concentration or a Rhodamine Calibration Dye
Solution.
3. Record the sensor output voltage (0 to 5 V).
4. Clean and dry the sensor.
5. Put the solid secondary standard on the optical end of the sensor.
6. Turn the solid secondary standard until it is aligned with the indexing mark on the sensor. A
clicking sound is heard when the solid secondary standard is correctly aligned.
7. Use the supplied screwdriver to turn the screw located on the side of the solid secondary
standard. Turn the screw until the sensor output voltage is the same as the value recorded.
Note: Turn the screw clockwise to increase the voltage. Turn the screw counter-clockwise to decrease the
voltage.
4.5 Sensor guidelines
Keep deposits off the sensor for best measurements. Examine the sensor to make sure that there
are no contaminants. Keep the substances shown in Table 3 away from the sensor.
Table 3 Contaminants
Substance Damage
Oil, sediment or biofilms Contaminates the sensor. If the sensor is contaminated, clean the sensor.
12 English

4.6 Maintenance
4.6.1 Clean the sensor
N O T I C E
To prevent damage to the sensor, do not use organic solvent solutions (e.g., acetone or methanol) to clean the
sensor. Organic solvents cause damage to the plastic housing and sensor lenses.
Clean the sensor after each deployment to remove unwanted material (e.g., oil, biological growth and
dirt). In addition, clean the sensor before and after calibration.
Adjust the sonde deployment time in accordance with how quickly sensor fouling occurs in the
deployment waters.
1. Clean the sensor with:
• Mild detergent
• Warm water
• Clean, soft toothbrush
• Soft cloth and/or cotton swabs
Note: Do not use dishwashing liquid to clean the sensor. Dishwashing liquid and household cleaners with skin
moisturizer can put a film on the sensor.
2. Rinse the sensor with clean water.
3. Soak the sensor in clean water for a minimum of 30 minutes.
4. Examine the optical windows. If unwanted material is seen on the optical windows, use optical
tissue or a cotton swab with soap and water to clean the optical windows.
5. Rinse the sensor with clean water.
6. If the substance cannot be removed, contact technical support.
4.7 Accessories
WARNING
Personal injury hazard. Use of non-approved parts may cause personal injury, damage to the
instrument or equipment malfunction. The replacement parts in this section are approved by the
manufacturer.
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to
the company website for contact information.
Description Quantity Item no.
Rhodamine Calibration Dye Solution, 21.33 ± 2.5 w/w% 236 mL (8 oz) 007273
Solid Secondary Standard 1 007726
Section 5 Central cleaning brush, HL7 (9195800)
5.1 Specifications
Specifications are subject to change without notice.
Specification Details
Maximum depth 200 m (656 ft)
Operating temperature –5 to 50 °C (23 to 122 °F), non-freezing
English 13

5.2 Product overview
N O T I C E
Do not manually turn the wiper arm because wiper motor damage can occur.
The central cleaning brush (9195800) is shown in Figure 5. The central cleaning brush cleans the
surrounding sensors at the start of the logging warm-up time.
Note: The central cleaning brush is only available for the HL7 sonde.
Refer to Sensor Settings in the online help to manually clean the surrounding sensors and to set the
number of revolutions in a cleaning cycle.
Figure 5 Central cleaning brush (9195800)
5.3 Maintenance
5.3.1 Clean the central cleaning brush
N O T I C E
To prevent damage, do not use solvents that dissolve plastic to clean the central cleaning brush.
Note: It may be necessary to clean the central cleaning brush more frequently if it is used in environments with
sediment (i.e., silt), mineral precipitate, biofilms, barnacles or pollutants such as oil.
1. Clean the central cleaning brush with:
• Mild detergent
• Non-toxic, degreasing solution, such as Simple Green® (A registered trademark of Sunshine
Makers, Inc.)
• Warm water
• Clean, soft toothbrush
• Soft cloth and/or cotton swabs
If necessary, soak the central cleaning brush in clean water for at least 30 minutes to make the
contamination softer and easier to remove.
Note: Do not use dishwashing liquid to clean the central cleaning brush. Dishwashing liquid and household
cleaners with skin moisturizer can put a film on the sensors.
2. Rinse the central cleaning brush with clean water.
3. If the substance cannot be removed, contact technical support.
5.3.2 Replace the brush
Replace the brush when wear or damage is seen on the brush. Refer to the documentation supplied
with the maintenance kit (9480900) for the central cleaning brush. Clean the central cleaning brush
before maintenance.
14 English

5.4 Replacement parts
Description Item no.
Maintenance kit, central cleaning brush 9480900
Section 6 Chloride sensor (007254)
6.1 Specifications
Specifications are subject to change without notice.
Specification Details
Range 0 to 18,000 mg/L
Accuracy ± 10% or ± 5 mg/L (the larger value)
Resolution 0.01 mg/L
Parameters Cl– or sensor mV
Deployment life of sensor cartridge 1 to 2 years
Life4 of sensor cartridge 1 to 2 years
Maximum depth 15 m (49 ft)
Operating temperature 0 to 50 °C (32 to 122 °F)
6.2 Product overview
The chloride sensor (007254) is green. Refer to Figure 6.
There is a replaceable sensor cartridge installed in the sensor. Refer to Specifications on page 15 for
the deployment life of the sensor cartridge.
The sensor cartridge has a depth limit of 15 meters (49 feet). Remove the sensor cartridge and install
the sensor cap before the sonde is deployed deeper than 15 m (49 ft) to prevent damage to the
sensor cartridge. Refer to the illustrated steps in Figure 7.
To extend the life of the sensor cartridge, remove the sensor cartridge and install the sensor cap
when the sensor is not in use. Refer to the illustrated steps in Figure 7.
Figure 6 Chloride sensor
1 Sensor 3 Sensor cap
2 Sensor cartridge 4 Glass vial for sensor cartridge storage
4Deployment life plus the storage life
English 15

Figure 7 Sensor cartridge removal
6.3 Calibration
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
Calibrate the sensors before initial use. At regular intervals, do a calibration check to make sure that
measurements stay within the tolerance range. Calibrate the sensors if measurements are out of the
tolerance range. The deionized (DI) water steps must be done for an accurate calibration.
Refer to the Hydrolab Operating Software Manual for instructions on how to do the tasks that follow:
• Start a sensor calibration.
• Start a sensor calibration check.
• Set the calibration mode.
• Select the measurement units shown during calibration and calibration checks.
• Look at the calibration history.
To look at the Hydrolab Operating Software Manual, start the Hydrolab Operating Software, click
Help and select Hydrolab Operating Software Help.
When a calibration or a calibration check is started, the step-by-step instructions for the sensor show
on the display.
6.4 Sensor guidelines
Keep deposits off the sensor for best measurements. Examine the sensor to make sure that there
are no contaminants. Keep the substances shown in Table 4 away from the sensor.
Table 4 Contaminants
Substance Damage
Oil, sediment or biofilms Contaminates the sensor. If the sensor is contaminated, clean the sensor.
16 English

6.5 Maintenance
6.5.1 Clean the sensor
N O T I C E
To prevent damage to the sensor, do not use organic solvent solutions (e.g., acetone or methanol) to clean the
sensor. Organic solvents cause damage to the plastic housing and sensor membrane.
N O T I C E
To prevent damage to the sensor membrane, do not use cleaning chemicals to clean the sensor membrane.
Clean the sensor after each deployment to remove unwanted material (e.g., oil, biological growth and
dirt). In addition, clean the sensor before and after calibration.
Adjust the sonde deployment time in accordance with how quickly sensor fouling occurs in the
deployment waters.
1. Clean the sensor with:
• Mild detergent
• Warm water
• Clean, soft toothbrush
Note: Do not use dishwashing liquid to clean the sensor. Dishwashing liquid and household cleaners with skin
moisturizer can put a film on the sensor.
2. Rinse the sensor with clean water.
3. Soak the sensor in clean water for a minimum of 30 minutes.
4. Examine the sensor cartridge membrane. If the sensor response is slow or unwanted material is
seen on the sensor membrane, clean the sensor membrane. If necessary, polish the sensor
membrane with 3M lapping film 261X (3.0 micron aluminum oxide particles) or an equivalent
abrasive.
5. Rinse the sensor with clean water.
6. If the substance cannot be removed, contact technical support.
6.5.2 Replace the sensor cartridge
Replace the sensor cartridge when it can no longer be calibrated or if the sensor cartridge is
damaged. Refer to the illustrated steps that follow.
English 17

6.6 Consumables and replacement parts
Consumables
Description Quantity Item no.
Low Chloride Calibration Standard, 4.62 mg/L 500 mL 013820HY
High Chloride Calibration Standard, 46.2 mg/L 500 mL 013830HY
Replacement parts
Description Item no.
Chloride sensor cartridge, green 003951
Sensor cap 003960
Section 7 Chlorophyll a sensors (007284, 007202, 9060600)
7.1 Specifications
Specifications are subject to change without notice.
Specification Details
Range 0 to 500 µg/L
Linearity R2 = 0.998
MDL ppb 0.05
MDL RFU 0.0003
Parameters µg/L, ppb, volts, RFU5
Maximum depth 200 m (656 ft)
Operating temperature –5 to 50 °C (23 to 122 °F), non-freezing
7.2 Product overview
N O T I C E
Do not put the sensor in red pH 4 buffer. Red pH 4 buffer can cause permanent damage to the sensor lenses. As
an alternative, use pH 4 buffer with no color.
The Chlorophyll a sensor is an optical fluorometer that determines the concentration of chlorophyll a
in water. Refer to Figure 8.
This sensor is sensitive to light. For the best results, point the end of the sensor away from light
sources.
Figure 8 Chlorophyll a sensor
5ppb is equivalent to µg/L. RFU is 0–100 based on the full detection range of the sensor.
18 English

7.3 Theory of operation
The sensor irradiates the water with blue light (460 nm). The chlorophyll a in the water absorbs the
blue light energy and gives red light (620 to 715 nm). The sensor directly measures the quantity of
red light in the water to determine the concentration of chlorophyll a. The reading is 0 to 500 µg/L (or
0 to 5 V).
7.4 Calibration
CAUTION
Chemical exposure hazard. Obey laboratory safety procedures and wear all of the personal protective
equipment appropriate to the chemicals that are handled. Refer to the current safety data sheets
(MSDS/SDS) for safety protocols.
CAUTION
Chemical exposure hazard. Dispose of chemicals and wastes in accordance with local, regional and
national regulations.
Calibrate the sensors before initial use, at regular intervals and after sensor maintenance or
modifications. Periodically, do a calibration check to make sure that measurements are still within
tolerance range.
Refer to the Hydrolab Operating Software Manual for instructions on how to do the tasks that follow:
• Start a sensor calibration.
• Start a sensor calibration check.
• Set the calibration mode.
• Select the measurement units shown during calibration and calibration checks.
• Look at the calibration history.
To look at the Hydrolab Operating Software Manual, start the Hydrolab Operating Software, click
Help and select Hydrolab Operating Software Help.
When a calibration or a calibration check is started, the step-by-step instructions for the sensor show
on the display.
7.4.1 Use a solid secondary standard
Use a solid secondary standard to verify the calibration of the sensor when a calibration check is
done. Refer to Accessories for ordering information.
Use a solid secondary standard only to verify the calibration of the sensor on which the value is set.
Set the value of the solid secondary standard as follows:
1. Make sure that the optical surface of the sensor is clean and dry.
2. Put the sensor into a sample with a known concentration or a Rhodamine Calibration Dye
Solution.
3. Record the sensor output voltage (0 to 5 V).
4. Clean and dry the sensor.
5. Put the solid secondary standard on the optical end of the sensor.
6. Turn the solid secondary standard until it is aligned with the indexing mark on the sensor. A
clicking sound is heard when the solid secondary standard is correctly aligned.
7. Use the supplied screwdriver to turn the screw located on the side of the solid secondary
standard. Turn the screw until the sensor output voltage is the same as the value recorded.
Note: Turn the screw clockwise to increase the voltage. Turn the screw counter-clockwise to decrease the
voltage.
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7.5 Sensor guidelines
Keep deposits off the sensor for best measurements. Examine the sensor to make sure that there
are no contaminants. Keep the substances shown in Table 5 away from the sensor.
Table 5 Contaminants
Substance Damage
Oil, sediment or biofilms Contaminates the sensor. If the sensor is contaminated, clean the sensor.
7.6 Maintenance
7.6.1 Clean the sensor
N O T I C E
To prevent damage to the sensor, do not use organic solvent solutions (e.g., acetone or methanol) to clean the
sensor. Organic solvents cause damage to the plastic housing and sensor lenses.
Clean the sensor after each deployment to remove unwanted material (e.g., oil, biological growth and
dirt). In addition, clean the sensor before and after calibration.
Adjust the sonde deployment time in accordance with how quickly sensor fouling occurs in the
deployment waters.
1. Clean the sensor with:
• Mild detergent
• Warm water
• Clean, soft toothbrush
• Soft cloth and/or cotton swabs
Note: Do not use dishwashing liquid to clean the sensor. Dishwashing liquid and household cleaners with skin
moisturizer can put a film on the sensor.
2. Rinse the sensor with clean water.
3. Soak the sensor in clean water for a minimum of 30 minutes.
4. Examine the optical windows. If unwanted material is seen on the optical windows, use optical
tissue or a cotton swab with soap and water to clean the optical windows.
5. Rinse the sensor with clean water.
6. If the substance cannot be removed, contact technical support.
7.7 Accessories
WARNING
Personal injury hazard. Use of non-approved parts may cause personal injury, damage to the
instrument or equipment malfunction. The replacement parts in this section are approved by the
manufacturer.
Note: Product and Article numbers may vary for some selling regions. Contact the appropriate distributor or refer to
the company website for contact information.
Description Quantity Item no.
Rhodamine Calibration Dye Solution, 21.33 ± 2.5 w/w% 236 mL (8 oz) 007273
Solid Secondary Standard 1 007726
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