ISG 601-502 User manual

601-502 - Microscope
Instructions
To ensure safety and obtain optimum performance and familiarize yourself with the use of this
microscope, we recommend that you read this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the microscope for further reference

Contents
1. Structure and nomenclature
2. Application
3. Technical parameter and specification
4. Configuration
5. Assemblage
6. Operation
7. Troubleshooting
8. Maintenance

Safety notice
1. Transportation
The microscope is a valuable instrument, handle with care, avoid impact or abrupt movement during
transportation. Do not push or pull the microscope during using, otherwise the optical precision may
be affected.
1_Hold the curve and keep the microscope in balance
2_Do not hold the focusing knob, eyepiece tubes and stage as these parts are movable.
Do not allow specimens or filters to fall off.
2. Working Environment
The microscope is a valuable instrument, improper use will make it reduce its optical precision.
1_Do not expose the microscope to direct sunlight
2_Temperature range is 0-40ºc and the max. humidity is 85%
3_High temperature and humidification can cause mold on the lenses
4_Avoid violent vibration as the vibration will reduce the optical quality
5_Place microscope on a stable surface and keep it balanced. Keep the environment well ventilated
and cover the microscope with the dust-cover
6_Do not place microscope in moist room to avoid short circuit.
3. Focusing Dials
Never turn the focusing dials in the adverse direction at the same time. Do not turn the coarse
focusing knob when the stage reaches max. position. These incorrect operations will make focusing
structure unworkable.
4. Clamp
The clamps fix the microscope firmly during transportation.
Please remove them before using.
5. Do not take the microscope apart
As it will be damaged. Such operations might have bad effect on the performance and may give the
an electric shock or injury. Please make contact with manufacturers if there are any problems.
6. Power Supply
Ensure the correct power input voltage is used. Incorrect input voltage may cause short circuit and the
unit burning out. Input voltage on the label: 240V 50/60Hz
7. Use the spare parts supplied by the manufactures
The bulb, fuse and electronic cord have been assembled already in the factory and please make sure
to use the spare parts of them supplied by the manufacturers. Improper bulb, fuse and electronic cord
could ruin the microscope and cause fire.

Spare bulbs: 3W LED lamp
Spare fuse: 250V 1A delayed model, 5 X 20 fuse
8. Mounting the bulb
Never touch the surface of the bulb with your hand directly. Use gloves or a cloth when you mount the
bulb to avoid leaving fingerprints. Fingerprints or stains should be wiped off with a tissue moistened
with a small amount of alcohol. The fingerprints will etch on the bulb's surface and make the
brightness lower and life cycle shorter.
Please make sure the bulb's touching points are Ok as it will not light or short circuit if the bulb's
touching points are damaged. Insert the bulb's legs into the bulb holder firmly. The bulb will go out if its
legs have not been inserted into the holder firmly. Such Operation may cause short circuit or smoking.
9. Temperature for illumination
When you use the halogen bulb, there will be high temperature when the bulb is lightened, never
move the collector when the bulb is lightened. Do not touch the bulb within 30 minutes after it has
been gout out. Make sure the bulb has been be cooling enough time (at least 30 minutes) to avoid
scald.
Do not put fibre, papers and incendive things (eg. Gasoline, aether, methanol and ethanol) close to
the bulb.
10.Make sure to turn off the power supply before assembling
microscope, replacing bulb or fuse.
11.Observing with oil immersion
Using small amount of oil immersion is enough. The redundant oil will adhere to stage or
condenser and these will reduce microscope’s performance. Get rid of the redundant oil or clean the
lens by using aether or pure alcohol according to this instruction. Pay attention during process as
these things are incentive. The instruction you buy may not including some products mentioned in this
instruction. Safety has been considered during design while users still have the possibility to be
injured and the instruction to be damaged when improper operation made. Please pay much more
attention to read this instruction carefully before using and keep it properly to make sure it will be got
when it is necessary.

12.
Mark
Meaning
1. Hot surfacr, do not touch with your hands
2. Read the instruction manual carefully before operating the microscope.
Unproper operation will cause hurt or damage to the operator and instrument
___
3. Switch on
○
4. Switch off
13.Operating the microscope only indoor and under the
adtitude 2000m, IP52 grads.
If the equipment is used in manner not specified by the manufacturer, the provided by the equipment
may be impaired.

I. Structure and nomenclature
1. Eyepiece; 2. Interpupillary scale ring; 3. Diopter adjusting ring; 4. Nosepiece; 5. Objectives; 6.
Mechanical stage; 7. Condenser fixing screw; 8. Condenser; 9. Collector; 10. Observation Head; 11.
Observation head fixing screw; 12. Microscope body; 13. Slide clamp; 14. Y-direction stage movement; 15.
X-direction stage movement; 16. Fine focusing knob; 17. Coarse focusing knob;
18. Iris diaphragm; 19. Trinocular tube

II. Application
The 601-502 biological microscope is designed for clinical experiments and routine medical
examination as well as for teaching and research purpose in biology, pharmaceutical, and bacteriology
observation in medical and hygienic establishments, chemical laboratory, research institute and
college, ect. This product, with modern design, good -looking appearance, adopts many advanced
structure &technology in order that the clients can operate the instrument conveniently and safely. The
following accessories can be optional for the customer, darkfield, phase contrast, photographic and
video accessories.

III. Technical parameter and specification
1). Specification
1. Optical system:
Mechanical tube length: 160mm
2. Illumination:
inserted transmitted illumination, 3W LED bulb
(standard outfit)
Input voltage: 100V~240V 50/60Hz
Voltage undulation 10%
Rating 100V~240V 50/60Hz 0.8A
Fuse 250V 1A delayed model, 5X20 mini type fuse 2pcs
3. Focusing adjustment:
Division of fine focusing adjustment: 0.002mm
Fine focusing knob control range: stage will be up or down 0.2mm per circle
Coarse focusing knob control range: stage will be up or down 36mm
4. Mechanical stage
X-movement range: 75mm, Y-movement range: 50mm
5. Nosepiece:
Roller bear quintuple nosepiece
6. Condenser:
Abbe NA=1.25 with iris diaphragm
7. Eye tubes:
Interpupillary range 55~75mm
8. Working environment:
Temperature: 0~40°
Humidity: max. 85%, no dew
2) Optical parameter
1, Objectives: achromatic objectives
Magnification
N.A.
Cover glass thinkness(mm)
W.D. (mm)
Dry/Oil
4X
0.1
0.17
26.9
Dry
10X
0.25
0.17
6.4
Dry
40X
0.65
0.17
0.6
Dry
100X
1.25
0.17
0.098
Oil
2, Eyepiece
WF10X/18mm

Glossary
1)Total magnification
Total magnification = Eyepiece’s magnification X objective’s magnification
2)Numerical aperture (N.A.)
N.A. value will affect resolution and image’s brightness, it is the leading parameter for
objectives. N.A.= n X sina
n stands for refractive index of the medium between objective and specimen or condenser (air
or oil immersion)
a stands for half angle of max. aperture angle in the axis
The image will be sharper and brighter when the N.A. value is bigger
3)Resolution
Resolution can be measured by the distance value from one point to another one which could
be distinguished on the object surface
Resolution=λ /(2XN.A.)
λstands for wavelength ( λ= 0.55um)
4)Working distance
The distance between the object surface to the first surface of optical system when you get
the sharpest image. The W.D. will become more short when the magnification for the
objective is bigger.
5)Eyepiece view field
The max diameter measured when the diaphragm is open. 10X/18 means the magnification is
10X and the max diameter of view field is 18mm
6)Effective view field
Liner view field which is observed on the object surface
Effective view field = eyepiece view field / objective’s magnification
7)Depth of field
Depth of field stands for depth of the space in which there is sharp image on the object surface.
Depth of field will be longer when the diaphragm reduces. Depth of field will be shorter when
the N.A. becomes bigger.
IV. Configuration

Item
Content
Piece
1
Microscope’s body ( including stage, nosepiece, coaxial coarse and
fine focusing adjustment, bracket for condenser, adjusting power 1pc
supply, LED bulb and normal collector)
1 set
2
Siedentopf binocular
1 pc
3
Condenser ( with iris diaphragm, without auxiliary lens)
1 pc
4
Objectives(achromatic objectives)4X10X40X100X
1 set
5
WF10X eyepiece
2 pcs
6
Blue filter
1 pc
7
Power cord
1 pc
8
Immersion oil
1 bottle
9
Dust cover
1 pc
10
Instruction
1 pc
11
Wrench 2.5, 1.5
1 set
Optional
Item
Content
1
Collector with iris diaphragm
2
Darkfield condenser
3
WF10X eyepiece with reticle
4
WF15X eyepiece
5
Plan objectives (4X, 10X, 40X, 100X)
6
6V 20W halogen illumination
7
Trinocular head

V. Assembly
Please read the safety notice carefully before assembly and make the assembly according to
the following steps.
Tools: wrench (2 pcs)
1. Checking the input voltage
There is correct input voltage in the label on microscope. Make sure the voltage in your
position is right. Improper input voltage may cause short circuit and fire. The microscope will
be damaged. Turn off the power supply (turn the switch to O ) and insert one end of the
electronic cord (connector) into the entrance for AC. Insert the other end of the electronic
cord (pin) into the grounded AC connector and make sure that the electronic cord has been
connected safely
_ Please use the supplied electronic cord by manufacturer
_ Please make sure to use PE electronic cord when using extra-long electronic cord.
_ Pay attention that the microscope should be placed near AC connector and the AC
connector is touchable for users
2. Removing the clamps for transportation
The clamps fix the stage and focusing adjustment firmly during transportation.
Handle the grooves beside collector and pull it out.
Remove the clamps by wrenches.
_ Mechanical stage
There is one clamp fixing the mechanical stage in
Y axis direction. Remove the bolts and clamps.
_ Focusing adjustment
Pull out the collector. The bracket is fixed by one clamp. Please remove the bolts and clamps.
3. Mount the binocular head
Loosen the fixing screw in the eye tubes and insert the binocular head into the eye tubes.
Then tighten the screw.
4. Mount the blue filter
Take out the filter from the bottom of condenser and mount it in the bracket then move the
bracket into the condenser.
5. Adjustment and replacement
1_Condenser
The condenser is mounted in the microscope before shipment. Remove or replace the
condenser according to the following steps
a. Handle the grooves of the condenser and pull it out
b. Turn the lifting knob for condenser and make the bracket in a suitable position
c. Loosen the screw in the left side of condenser and take out the condenser. Mount the blue
filter in the bracket then move it into the bottom of the condenser. Make the label in the
condenser forward and move the condenser into the bracket. At last tighten the screw.
d. Handle the grooves and push the condenser in.
e. Turn the lifting knob of condenser and make the condenser to the highest position.

f. Turn the lifting knob of condenser and make the condenser a lit bit lower. The image will be
focused in the right place of object (center of light path) when the light transit condenser.
Move the condenser up and down a little to make the dispersion image disappeared.
2_Objectives
The objectives have been mounted in the microscope before shipment
Take off the specimen from the stage and make the stage lower when you replace the objectives.
Handle the objective with your two hands and remove it. Be carefully do not make
it fall off. Turn the nosepiece to get the objective you need. The magnification for the objectives
becomes bigger in clockwise direction (observing from the top of the microscope).
3_Specimen clip
The clip has been mounted in the stage before shipment
Loosen two screws by wrenches supplied by manufacturer to remove the clip.
4_Eyepieces
10X eyepieces have been in the foam box before shipment. Insert the eyepiece into the eyepiece
tubes and tighten the fixing screws by wrench
5_Other attachments
Replace other attachments (eg, camera) according to the instruction
6. Replacement
1_Replacement for bulb (halogen bulb, optional)
Turn off the power supply (turn the switch to _O_) and pull out the connector
Wait for 30 minutes till the bulb is cooling
Handle the grooves in condenser and pull it out
Pull out the original bulb
Replace the bulb by gloves or cloth material then insert the bulb into the socket entirely
Pull the condenser back to its original position
Connect the electronic cord to the power supply
2_Replacement for fuse
Turn off the power supply (turn the switch to O) and pull out the connector
Open the cover for fuse by screwdriver
Mount the new fuse
Make sure that the voltage shown in the cover for fuse is same to the working voltage
Mount the cover
VI. Operation

1. Illumination
Turn on the power supply (turn the switch to -), then the bulb will be on. The lightness can be adjusted
by turning the adjusting knob.
2. Interpupillary range
Adjust the siendentopf binocular to make sure that view field in the right and left eyepiece is consistent.
3. Placing the specimen
Put the specimen in the stage and make the cover glass on it. Make the clips clamp them entirely. Two
slides can be put in the stage in the same time
4. Focus by 10X objective
Put the 10X objective into the optic path and focus by turning coarse and fine focusing knob
_ Turn the stage and focusing knob according to the direction shown in the picture
_ There is no coarse focusing knob in one side of stage’s moving knob and in the other side
there are both coarse and fine focusing knob
_ Never turn the left and right focusing knob in the adverse direction at the same time. Never
turn the coarse focusing knob when the stage is in the end of removing range. Otherwise it will make
damage to the instrument
_ Detailed operation
It is difficult to do focusing if turning the focusing knob optionally. The specimen may be crushed when
100X objective is used. Please read the instruction carefully to avoid making damage to the cover
glass or objective
1_Put the 10X or 4X objective into optic path
2_Remove the stage to the top by turning the coarse focusing knob
3_Observe through the eyepiece and turn the coarse focusing knob slowly to let the stage down and
down till there is sharp image
4_Turn the fine focusing knob to focus finely Use the 10X objective or 4X objective firstly before 40X
and 100X objective. Turn the fine focusing knob to focus finely
5. Adjusting the diopter for eyepieces
Adjust the diopter ring according to user’s diopter for left and right eyes. This function could make use
of advantages of objectives fully. Meanwhile it also can be react as focusing
1_Put the 40X objective in the optic path and turn the coarse and fine focusing knob to do focusing
2_Put the 10X objective (or 4X objective) in the optic path
3_Let your left eye observe through the left eyepiece and do focusing by the diopter adjusting ring in
left eyepiece
4_Repeat the above steps till sharp image can be observed by left and right eyepiece at the same
time.
6. Adjusting the upright position for condenser
Turn the lifting knob for condenser till it reaches the top position then fall it a little bit down. If there is
dispersion image in the view field, please remove the condenser a little bit of up and down to make the

dispersion image disappeared
7. Objectives
Turn the nosepiece to choose the objective and adjust the view field diaphragm and aperture
accordingly
8. View field diaphragm
Adjust the view field diaphragm according to the objective till it_ image touches the edge of
view field as this could eliminate the outside light and increase the contrast of the image.
Notice: There will no image for view field diaphragm when 100X objective is used. So adjust
the diaphragm to it’s min diameter
Centering view field diaphragm
1_Use the 10X objective and focus the image. Turn the view field diaphragm adjusting
ring with ershins and reduce the diaphragm’s diameter
__Turn the lifting knob for condenser to focus the image of view field diaphragm
__Turn the two centering screws for condenser and make the image in the center of
view field
__Check the centering and open the view field diaphragm till the image of the
diaphragm touches the edge of view field. Please do the centering again if the image
of the diaphragm doesn’t touch the edge of view field
__Open the view field diaphragm and make its image a little bigger than the view field in practice
9. Adjusting aperture diaphragm
Adjust the aperture diaphragm adjusting shaft for condenser till the position which stands for
the magnification of objective
_ adjusting the aperture diaphragm
1_Adjust the aperture diaphragm by the adjusting shaft. The brightness and resolution will
be decrease while the contrast and depth of view will increase if the diameter of aperture
diaphragm reduces
2_Adjust the value of aperture diaphragm to 70%-80% of the value of objective’s N.A.
3_The aperture diaphragm could control the N.A. of condenser. Do not adjusting the
brightness by it while use the brightness adjusting knob to make the adjustment
4_N.A. is marked in each objective
e.g. 40X/0.65 stands for that magnification is 40X and N.A. is 0.65
5_Remove the eyepiece and observe through the eye tubes by eyes directly
N.A. of condenser could tell users the position of aperture diaphragm adjusting shaft in
corresponding magnification. (That is to say, the value of aperture diaphragm should be
70% to 80% of the N.A. of objective when the aperture diaphragm adjusting shaft reaches
some position) If the objective has been changed, please move the shaft to the position with
the same value which is in the objective in the optic path and this will make ideal contrast
10. Observing with oil immersion
There is_ oil_ marked in the oil immersion objective. Please use the oil immersion supplied
by the manufacturer between the objective and cover glass.

1_Operation
Condenser_
Remove the slide back and fall the condenser down a little.
One drop of oil could be placed in the top of condenser by through the long aperture of stage.
Then move the slide forward and lift the condenser up
Objectives_
Turn the nosepiece and take the objective out of the optic path. Place one drop of oil on the slide then
turn the condenser slowly and mount the objective.
2_Air bubble in oil immersion
a) Never make the oil immersion into your eyes. Please take following steps as soon as the
oil immersion touches your skin or eyes.
Use soap or clean water to wash your skin carefully.
Please wash your eyes with clean water (at least wash 15 minutes) and go to the hospital
at once.
b) Do not expose the oil immersion in the sun or ultraviolet radiation directly.
As the air bubble in the oil immersion will make bad affect to the quality of image, please
make sure that there is no air bubble in the oil immersion before use. Please check the air
bubble as following, remove the eyepieces and open the view field diaphragm and aperture
diaphragm entirely then observe the exit pupil which is light and in nummular shape.
c) Remove the air bubble as following.
Turn the nosepiece slowly and turn the oil immersion objective once or twice.
Turn the lifting knob for condenser slowly and make the condenser a little bit of up and
down.
Increase the amount of oil immersion or change the old oil immersion with new one
3_Operation for oil immersion
Use the oil immersion as little as possible. The oil immersion will be conglutinated to the
stage and condenser and this will have bad effect the performance if there is too much oil
immersion. Please clean the redundant oil on the objective and condenser after finishing
the observation otherwise the image will be affected. Use the aether to clean the oil then
use the pure alcohol (ethanol or carbinol) to make entirely cleaning. Please repeat the clean
three or four times.
_ Notice:
Please follow the instruction from manufactures when the aether or pure alcohol is used.
Keep them away from the fire or electronic spark
4_Attention
_ Keep the bottle for oil immersion sealed as much as possible and check it periodically
_ Never press the bottle overly as it may caused oil gushed out of the bottle
_ Clean the out surface of the bottle from remanent oil
11.Adjusting the coarse focusing knob’s tension

The tension of coarse focusing knob is adjustable. Please turn the tension controlling ring of
the coarse focusing knob clockwise then the tension will be increased. This ring is near the
coarse focusing knob. If you want decrease the tension, please turn the ring with ershins. The
stage will fall down automatically if the tension of the ring decreased too much
VII. Trouble Shooting
If difficulties should be encountered in the course of operation, and no major instrument
mal functions can initially be detected, please recheck the symptoms, referring to
the tables provided below, before contacting the service department representative.

1. optical
Symptoms
Causes
Countermeasures
Darkness at the preiphery,
or uneven viewfield
brightness
Revolving nosepiece not in click
stop position ( objective not
centered in optical path )
Revolve to click-stop position
( switch the objective
correctly into optical path )
Filament image not in center
Centering
Dirt or dust on the lens
( condenser, objective, eyepiece
or collector )
Cleaning
Dirt or dust in the viewfield
Dirt or dust on the lens
( condenser, objective, eyepiece
or collector )
Cleaning
Dirt or dust on the slide
Cleaning
Condenser position too low
Correct position
Poor image quality ( low
resolution poor contrast )
No cover glass on the specimen
Attach cover glass
Cover glass too thick or thin
Use cover glass of specified
thickness ( 0.17mm )
Slide upside-down
Turn over the slide
Immersion oil on dry objective
( especially 40X )
Cleaning
No immersion oil used on
immersion objective
Use immersion oil
Dirt or dust on the lens
( condenser, objective, eyepiece
or collector )
Cleaning
Air bubbles in immersion oil
Remove air bubbles
Unspecified immersion oil used
Use specified immersion oil
Condenser aperture and field
diaphragm opened too far
Close properly
Dirt or dust on the entrance lens
Clearing
Condenser position too low
Raise to the position where
the field diaphragn image in
focus
Condenser not in the center of
the view field or condenser
inclined
Reinstallation condenser and
carefully adjust with center
screw
Image shifts on one side
Revolving nosepiece not in click
stop position
Adjust the nosepiece and
centering
Floating specimen
Fasten securely
Image shifts during focus
Specimen rise from stage
surface
Place it stable
Revolving nosepiece not in click
stop position
Revolving to click-stop
position

Image tinged yellow
Blue filter not used
Use blue filter
Insufficient illumination
brightness
Condenser aperture too small
Re-adjust the aperture
Condenser position too low
Correct positioning
2. Mechanical
Symptoms
Causes
Countermeasures
Image not focusable with
high power objectives
Slide upside-down
Turn slide over
Cover glass too thick
Use coverglass of specimen
thickness ( 0.17mm )
High power objective
contacts slide when
changed-over from low
power
Slide upside down
Turn slide over
Coverglass too thick
Use coverglass of specimen
thickness ( 0.17mm)
Specimen movement
unsmooth
Mechanical stage not securely
fastened
Tighten all fastener
Binocular images not
integrated
Interpupillary distance not
correctly adjusted
Adjustment
Excessive eye fatigue
Incorrect diopter adjustment
Correct adjustment
Inadequate brightness or
illumination
Adjust brightness with control
dial
3. Electrical
Symptoms
Causes
Countermeasures
Lamp does not light when
switched on
No electrical power
Check power cord connection
Lamp bulb not inserted
Insert correctly
Lamp failure
Replacement
Sudden lamp failure
Unspecified lamp bulb used or
input voltage too high
Replace with specified lamp
bulb. If the same symptom
occurs after replacing the
lamp, contact your dealer
Insufficient illumination
brightness
Unspecified lamp bulb used
Replace with specified lamp
bulb
Voltage too low
Increase brightness with
control knob
Flickering or unstable
lamp brightness
Lamp bulb about to fail
Replacement
Lamp bulb not correctly inserted
into socket
Check for positive connection
VIII. Care and Maintenance
1. Cleaning Lenses
Dust is best removed with a soft brush or gauze.

More persistent dirt, such as fingerprints, grease and oil, may be removed with soft
cotton, lens tissue, or gauze lightly moistened with absolute alcohol (ethyl or methy
alcohol).
To clean immersion oil off the oil-immersion type objective, use lens tissue, soft cotton
or gauze lightly moistened with petroleum benzine only.
Do not use petroleum benzine to clean the entrance lens at the bottom of the eyepiece
tube or prism surfaces inside the eyepiece tube.
Absolute alcohol and petroleum benzine are quite inflammable. Take great care when
handing them and when setting the power switch on and off. Be very careful with fire.
2. Cleaning painted or plastic surfaces
Avoid use of and organic solvents (such as alcohol, ether, thinner, ect.) to clean the
painted or plastic surfaces of the instrument. We recommend the use of silicon cloth.
More persistent dirt may be cleaned with mild detergent solution.
Printed plastic surfaces should be cleaned only by soft cloth moistened with water.
3. When not in use
When the microscope is not in use, cover it up with dust cover, and store in a dry
place not subject to mold.
We especially recommend that the objectives and eyepieces be kept in a container
( such as a desecrator).
4. Periodical inspection
To maintain the performance of the microscope, periodical inspection is recommended.
International Scientific Group
Unit 1, Karoo Close
Bexwell Business Park
Bexwell
Norfolk
PE38 9GA
[phone]
[e-mail]