Knauer BlueShadow Series User manual

BlueShadow
Valves and valve drives
Manual
V7695A
HPLC

3Tableofcontents
Table of contents
Note: For your own safety, be sure to read the manual and
always observe the warnings and safety information on the
device and in the manual!
Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Overview valves and valve drives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Multiposition valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Injection valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Connections of the ports at the positions loading and injecting . . . . . . . . . . . 8
Series and operating modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Laboratory use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Where is it prohibited to use the device or system? . . . . . . . . . . . . . . . . . . . . . 10
Application examples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Selection of a column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Column selection with a 2-channel valve . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Backflushing of a column . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Backflushing of a precolumn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Accumulation of a sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Precleaning of a sample by a precolumn . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Precleaning of a sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Alternating precleaning of a sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Symbols and labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Laboratory regulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Solvents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
PEEK connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Protective measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Power supply and mains connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Target group . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
To what should the user pay particular attention? . . . . . . . . . . . . . . . . . . . . 23
What expertise should users have to safely operate a HPLC device
or system? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Protective film on the screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Scope of supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Checking the scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Space requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Installation site . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Front view of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Device display and buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Rear view of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Manual valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Electrical valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
V7695A

4Tableofcontents
Mounting of the valve on the valve drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Screw the adapter plate to the back side of the valve. . . . . . . . . . . . . . . . . . 27
Screw the valve onto the valve drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Mounting plate for valves and column holder . . . . . . . . . . . . . . . . . . . . . . . . . 29
Connecting capillary with valve and seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Inserting the needle seal in the syringe connection . . . . . . . . . . . . . . . . . . . . . 30
Multifunction module ‘Assistant’ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Connection of the pump with other devices . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Control of the pump with chromatography software . . . . . . . . . . . . . . . . . 32
Local area network and automatic configuration . . . . . . . . . . . . . . . . . . . . . 32
Interfaces for data transfer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Remote terminal strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Operating the valves and valve drives . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Switch-on and self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Display of the valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Control of the valve with chromatography software . . . . . . . . . . . . . . . . . . . . 36
Operating the valve drive manually . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
The keyboard for operating the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Setting the position of the valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Set the position manually on the valve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Filling the sample into the sample loop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Injecting the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Operating the valve drive externally . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Analog control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Binary control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Connecting the flat ribbon cable with the connector strip . . . . . . . . . . . . . 40
Connect valve drive to the devices Manager or Interface Box. . . . . . . . . . . . 41
Maintenance and care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Removing the valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Alignment of the rotor seal in the valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Insert new needle seal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Increasing the pressure stability of valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Cleaning and caring for the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Environmental protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Ambient storage conditions for the device . . . . . . . . . . . . . . . . . . . . . . . . . 48
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5Tableofcontents
Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Valve drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Electrical valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Manual valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Abbreviations and terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Legal information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Warranty conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Manufacturer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Table of Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6Intendeduse
Intended use
HPLC High-pressure liquid chromatography (HPLC) is a method 
for separating substance mixtures, determining substances, 
and measuring their concentration.
The device or system is suitable for high-pressure liquid chroma-
tography. It is suitable for laboratory use, for analyzing sub-
stance mixtures that can be dissolved in a solvent or solvent
mixture.
Injection valves,
multiposition valves,
and
electrical
valve drives
Injection valves or multiposition valves are used in the HPLC to
bring in the sample from the sample loop in the pump's high
pressure flow, so that the sample is transported to the column.
The sample is fed in at atmospheric pressure in the sample loop
by a syringe or via feed pump from a sample reservoir.
Injection valves can have 2 or 3 channels, which depends on
the rotor seal. Multiposition valves are 1-channel valves.
Overview valves and valve drives
Valves and valve drives are always completely assembled and
tested when they leave the factory. The following components
may be included:
Fig. 1 Overview valves and valve drives
Main areas of
application
Main areas of application for multichannel valves are the injec-
tion or column switching, the column backflushing, sample
accumulation, or the alternating sample accumulation. Multi-
channel valves are used for example to select different solvents
(solvent selection valve) without having to reestablish the con-
nections anew or laying out the capillaries again.
Single-channel valves are employed for fractioning if the form
and the size of the fraction containers should be freely select-
able. By combining a maximum of eight single-channel valve,
up to 120 positions can be used for fractioning in applications
in preparative HPLC.
Legend
A. Mounting plate
B. Valve drive
C. Mounted, electrical
injection valve
D. Valve holder for
pumps or detectors
E. Electrical injection
valve
F. Hand injection valve
A
B
C
D
E
F

7Intendeduse
Multiposition valves
Structure and function The multiposition valve is used in chromatography, for example
in the following cases:
Selection of up to 13 different solvents
Fractioning
Column switching up to 16 columns
Fig. 2 Diagram of a multiposition valve 7P/1C
Injection valves
Versions 6 Ports with 3 channels
6 Ports with 2 channels
The structure of the rotor seal in the inside of the injection valve
specifies whether the valve can be operated as a 2- or 3-channel
valve.
Fig. 3 Injection valves 6P/2C and 6P/3C
Legend
A. Position number
B. Connection
between the central
connection and the
individual positions
1–6
C. Adapter plate for
mounting on the
valve drive
4
62
53
1
ABC
Legend
A. Position number
B. 6 Ports with 2 chan-
nels (6P/2C)
C. Adapter plate for
mounting on the
valve drive
D. 6 Ports with 3 chan-
nels (6P/3C)
1
4
62
53
1
4
62
53
ABCD

8Intendeduse
Connections of the ports at the positions
loading and injecting
Valve position
Loading (L)
In the valve position loading, the sample is filled into the sample
loop.
Valve position
Injecting (I)
In the valve position injecting, the sample is flushed for example
on a precolumn or main column for preseparation or separation.
The sample loop is filled, by injecting the sample at connection
1. This runs into the sample loop (connection 2 and 5) and
excessive sample is transported into the waste container
through connection 6.
Fig. 4 Valve positions loading and injecting
The eluent flows through the sample loop in opposite direction
during the injecting, see arrows in the figures.
Reed contact The reed contact in the injection or multiposition valve gives the
start signal for a measurement to the entire analytical system
or a detector.
Hand-operated valves For hand-operated valves, a mounting of a mounting plate or
a universal angle on the housing of a valve drive is advisable.
Electrical
valve drives
Electrical valve drives for the injection or multiposition valves
synchronize the sample injection in the analytical system. The
valves are operated by a 24 V direct current motor. The electri-
cal drive and the valve have been factory-set and can be modi-
fied by DIP switches (Dual In-line Package) on the bottom of the
device for certain applications.
By means of chromatography software or a cable for remote
control, certain positions can be controlled directly on a valve.
Delivery ex works All valve drives are delivered with a ready-mounted injection
or multiposition valve.
Loading Injecting
1
2
3
4
5
6
Waste
Pump
Column
Sample
1
2
3
4
5
6
Waste
Pump
Column
Sample

9Intendeduse
Series and operating modes
The following valves fit to the listed valve drives:
Note: The possible combinations between valves and electri-
cal drives can be queried by the technical support of
the manufacturer.
Valve Switch posi-
tions
Operating mode
6V injection valve 2 6P/3C
6 ports/3 channels
6P/2C
6 ports/2 channels
6V Multiposition
valve
67P/1C
7 ports/1 channel
12V Multiposition
valve
12 13P/1C
13 ports/1 channel
16V Multiposition
valve
16 16P/1C
16 ports/1 channel
4
62
53
1

10 Intended use
Room ventilation,
A/C system,
sunlight
Always use the device in rooms that are well-ventilated, and are
preferably equipped with an air-conditioning system. When set-
ting up the system at the installation location, make sure that it
is protected against direct sunlight.
Checking intended use Only use the device for applications that fall within the range
of the intended use. Otherwise, the protective and safety
equipment of the device could fail.
Laboratory use
Biochemistry analyses
Chemical analyses
Food analyses
Pharmaceutical analyses
Environmental analyses
Where is it prohibited to use the
device or system?
DANGER! Risk of explosion! Never use the device in poten-
tially explosive atmospheres without appropri-
ate protective equipment and approval by a
notified body! Inform the technical service
department of the manufacturer.
Application examples
Abbreviations Connections and channels of an injection or multiposition valve
are described by abbreviations.
Example 6P/3C valve: That is an injection valve with 6 connec-
tions (P = port) and 3 channels (C = channel).
6P/3C valves Selection of a column
Backflushing of a column
Backflushing of a precolumn
Sample accumulation on a precolumn
Alternating accumulation of a sample
Alternating precleaning of a sample
6P/2C valves Preseparation and analyses on several columns
after each other
Sample precleaning by a precolumn
7P/1C valves Series switching of up to 16 columns
Step gradient or selection of a eluent

11 Intended use
Selection of a column
Basics The selection of a column, also called column selection, is used
in chromatography, for example in the following cases:
Method development
Column selection
Injection From the injection valve, the sample is injected via the channel
2->1 onto the column A. From connection 5, the components of
the sample are forwarded separate to the detector.
Fig. 5 Column selection – injection from the 6P/3C valve
on column A
Note: Make sure there is no pressure when switching
between the columns so that the columns are not
damaged.
From the injection valve, the sample is injected via the channel
2->3 onto the column B. From connection 5, the components of
the sample are forwarded separate to the detector.
Fig. 6 Column selection – injection from the 6P/3C valve
on column B
Injecting on column A
1
23
4
56
Column B
Column A
Detector
Injection
Injecting on column B
1
23
4
56
Column A
Column B
Injection
Detector