LiCONiC StoreX STX40 User manual

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Operating Manual
for
StoreX
Precision Incubator
Series
STX40, STX100, STX200, STX500, STX1000
IC, HC, HR, DC, DR, DF
SA, BT, IT
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StoreX Series STX_V078.doc 2CMa, 23.11.2009
Important Notice
©1993-1995, 1995-1999, 2000, 2001, 2002 2008 LiCONiC AG Liechtenstein, all rights
reserved.
The Information contained in this document is subject to change without notice.
Information provided by LiCONiC AG is believed to be accurate and reliable. However, the
user is responsible for the proper and correct use of the product. No license is granted by
acquisition of the product for any patent or patent rights of LiCONiC. If the user does not
follow the instructions given in this manual, LiCONiC does not take any responsibility for
injuries or damages caused by the LiCONiC product.
Contact Address
LiCONiC AG
Industriestrasse 8
FL - 9493 Mauren
Principality of Liechtenstein
Telephone: [phone]
Telex: [phone]
E-mail: [email protected]
Internet: www.liconic.com
Before operating the instrument, the user must read and understand this
manual.
Installation and Servicing
Installation, servicing and reinstallation of the instrument shall only be performed by System
Integrators and / or service personnel authorized by LiCONiC AG.
LiCONiC regularly offers Operator Training Courses. We highly recommend the attendance
of such a course prior to working with a LiCONiC Instrument.
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StoreX Series STX_V078.doc 3CMa, 23.11.2009
Table of Contents
1. About the Product and its Manual .............................................................................5
1.1 Intended Use .................................................................................................................5
1.2 Unintended Use............................................................................................................. 5
1.3 Product Identification ..................................................................................................... 5
1.4 Scope of Delivery .......................................................................................................... 5
1.5 General..........................................................................................................................5
1.6 Definitions......................................................................................................................6
1.7 Target User Group......................................................................................................... 6
1.8 Importance of the Safety Instructions ............................................................................ 6
1.9 Labels ............................................................................................................................6
1.10 Safety - Basics.............................................................................................................. 7
1.11 General Safety Regulations.......................................................................................... 7
2. The StoreX Incubator Family......................................................................................8
2.1 StoreX Instruments........................................................................................................8
2.2 LiCONiC StoreX Features .............................................................................................8
2.3 StoreX Configuration and Capacity Overview ............................................................... 9
2.4 LiCONiC StoreX Climate Overview ............................................................................. 10
2.5 The StoreX 40 Handler................................................................................................ 11
2.6 The StoreX Carrousel Handler ....................................................................................12
3. Options.......................................................................................................................13
3.2 StoreX Climate Options ............................................................................................... 13
3.3 StoreX Configurations ................................................................................................. 13
3.4 RH-Option.................................................................................................................... 15
3.5 Gassing Options .......................................................................................................... 16
3.6 UHS (Ultra High Speed) Option...................................................................................19
3.7 Customized Color ........................................................................................................ 19
4. Accessories ...............................................................................................................20
4.1 STX Stackers............................................................................................................... 20
4.2 STX Transfer Stations .................................................................................................21
4.3 Slide Station ................................................................................................................23
4.4 Lift Station....................................................................................................................23
4.5 Shuttle Station .............................................................................................................24
4.6 Bar Code Reader......................................................................................................... 26
4.7 Scheduler ....................................................................................................................27
5. Packing and Transport..............................................................................................28
5.1 Unpacking the Instrument............................................................................................ 28
6. Installation of the Instrument...................................................................................30
6.1 Transfer Station Position .............................................................................................33
6.2 Electrical Connections ................................................................................................. 37
6.3 Communication Connections....................................................................................... 38
6.4 Gassing Installation .....................................................................................................40
6.5 About other Hazardous Substances............................................................................ 55
7. Manual Operation......................................................................................................57
7.1 Routine Safety Checks ................................................................................................ 57
7.2 Front Door ...................................................................................................................58
7.3 Glass Door................................................................................................................... 59
7.4 Stackers.......................................................................................................................60
7.5 Bar Code Reader......................................................................................................... 63
7.6 The Robot Gate ........................................................................................................... 63
7.7 Humidity Reservoir ...................................................................................................... 64
7.8 Power-On System .......................................................................................................65
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StoreX Series STX_V078.doc 4CMa, 23.11.2009
7.9 Alarm System ..............................................................................................................66
7.10 Temperature Settings .................................................................................................66
7.11 Cleaning...................................................................................................................... 67
7.12 Disinfecting .................................................................................................................67
7.13 Defrost ........................................................................................................................67
8. Remote Operation .....................................................................................................68
8.1 RS-232 Serial Port Configuration ................................................................................68
8.2 Command Transmission Procedure ............................................................................ 68
8.3 Commands ..................................................................................................................73
8.4 Handling Status / Error Messages............................................................................... 78
8.5 Program Examples ...................................................................................................... 81
9. Utility Software ..........................................................................................................84
10. Troubleshooting........................................................................................................88
11. Maintenance...............................................................................................................89
11.1 Maintenance Guidelines .............................................................................................89
11.2 Removal of StoreX Handling ...................................................................................... 90
11.3 Lubrication of Handling Components..........................................................................91
11.4 Cleaning...................................................................................................................... 91
11.5 Bar Code Scanner ......................................................................................................92
11.6 Lubrication ..................................................................................................................92
11.7 Belt Tension................................................................................................................92
12. Technical Data...........................................................................................................93
12.1 Mechanics................................................................................................................... 93
12.2 Electronics ..................................................................................................................93
12.3 Pneumatics ................................................................................................................. 93
12.4 Overall Dimensions..................................................................................................... 93
12.5 Weights.......................................................................................................................94
12.6 Ambient Ratings ......................................................................................................... 94
13. Appendix....................................................................................................................95
13.1 Decontamination instructions......................................................................................95
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StoreX Series STX_V078.doc 5CMa, 23.11.2009
1. About the Product and its Manual
1.1 Intended Use
The LiCONiC StoreX is intended for storage, identification and transfer of
Microplates. It is to be applied exclusively in the research field. The LiCONiC
StoreX is intended to work as an element in an automated system.
1.2 Unintended Use
Due to its open architecture, the LiCONiC StoreX is NOT intended for clinical and
diagnostic applications. These applications would be carried out by less qualified
people, exposing them to dangerous liquids in the case of instrument
malfunction.
Read the safety instructions in this Manual carefully.
1.3 Product Identification
The Type Plate is located at the rear of the LiCONiC StoreX
For future reference, please read the instrument Serial No. on the rear of your
instrument and write it in the provided space.
1.4 Scope of Delivery
• Basic Unit, consisting of the Plate Handling, the Gate and the Incubation
chamber
• Product Documents
• Cables
• User Software and Drivers
• Stackers (as specified)
• Transfer Station (as specified)
1.5 General
The LiCONiC StoreX is not a complete product for end use. It is delivered
exclusively to professional System Integrators who take full responsibility for safe
installation and documentation of the entire system.
Do not operate until it has been established that the system, into which the
LiCONiC StoreX has been incorporated is in compliance with all local safety
regulations.
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StoreX Series STX_V078.doc 6CMa, 23.11.2009
1.6 Definitions
Operator: Any person who uses the equipment for its intended
purpose.
System Integrator: Authorized person or company responsible for
installation, initial start up and overall safety of the
system.
Person or company who carries out service and
maintenance tasks and is therefore contacted in the
case of any problems with the system.
1.7 Target User Group
The StoreX has been conceived for applications in the research field, requiring
highly qualified and authorized laboratory personnel.
Instrument operation requires thorough knowledge of applications, instrument
functions and software programs as well as all applicable safety rules and
regulations.
1.8 Importance of the Safety Instructions
This chapter contains general information assuring safe operation of the
instrument. More specific instructions in regard to safety are given throughout this
manual, at the respective points where observation is most important.
Make sure that all Safety Instructions in this publication are strictly followed.
1.9 Labels
Throughout this publication, Warning Notices are to be interpreted as follows:
WARNING !
Indicates the possibility of severe personal injury, loss of life or equipment
damage if instructions are not followed.
CAUTION !
Indicates the possibility of severe equipment damage if instructions are not
followed.
NOTE !
Gives helpful information about the equipment.
DANGER !
May be harmful to your eyes.
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StoreX Series STX_V078.doc 7CMa, 23.11.2009
1.10 Safety - Basics
1.10.1 General Operating Hazards
Carefully observe the following precautions:
1.10.2 Mechanical Hazards
Keep the housing and safety panels closed and never reach into the instrument
work space when the unit is in operating mode.
1.10.3 Chemical, Biological and Radioactive Hazards
All samples and test-kit components must be considered as potentially
hazardous agents. Therefore, a potential risk may arise from the liquids being
handled, such as infectious biological samples, toxic or corrosive chemicals, or
radioactive substances. Strictly apply appropriate safety precautions according to
local, state and federal regulations.
Prior to any first time application, test runs should be made with a neutral liquid to
allow optimization of all liquid handling parameters.
Handling and disposing of waste has to be in accordance with all local, state and
federal environmental, health and safety laws and regulations.
Prior to executing any maintenance task on the instrument or sending it, or parts
of it for repair, the instrument or the parts have to be thoroughly decontaminated.
1.11 General Safety Regulations
1.11.1 Legal Requirements
Please consult the Manufacturer's Declaration, delivered with each unit by
LiCONiC AG, listing all applied directives and standards.
In Europe, the System Integrator must provide a Conformity Declaration upon
final installation of the entire system.
1.11.2 General Inspection and Maintenance Duties
Only an authorized System Integrator shall carry out inspection, maintenance
and repair tasks.
1.11.3 Spare Parts to be used
Only original LiCONiC Spare Parts may be used. If other parts are used during
the normal warranty period, the manufacturer's guarantee may be invalidated.
1.11.4 Modifications
Modifications shall only be carried out by an authorized System Integrator.
LiCONiC AG will not accept responsibility for any claim resulting from
unauthorized modification or alteration.
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StoreX Series STX_V078.doc 8CMa, 23.11.2009
2. The StoreX Incubator Family
2.1 StoreX Instruments
The StoreX Series is the first compact Climate Storage with Integrated Handling
that covers the whole range of climates in laboratory applications. The StoreX
Series not only covers a wide temperature range, it also offers storage and
processes from ultra-dry to extreme humidity. A variety of gas options are
available.
The modular mechanical design in combination with extremely simple commands
eases the integration of StoreX into any environment. A growing number of
accessories are available for the StoreX Series. The combination of
environmentally controlled Storage and Automatic Plate Exchange Capability
make StoreX the Rex (Latin: king) of storage in laboratory applications.
2.2 LiCONiC StoreX Features
All StoreX units have the same compact dimensions. StoreX has a user front
door which allows comfortable and easy access for manual operation. The
internal glass door allows visual inspection of the content and operation of the
system without disturbing the internal climate. Removable stackers make the use
of the StoreX even simpler and more efficient. Stackers are available for all
common plate types.
Front User Door on the
front for manual access
Removable Stackers
Small Robotic Gate on
th
e
r
ear
Integrated plate transfer
handler
Single Port Complete
Remote Access
O
p
eration
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StoreX Series STX_V078.doc 9CMa, 23.11.2009
2.3 StoreX Configuration and Capacity Overview
The Family of StoreX is structured by their configuration, capacity in Microtiter
plates and their climate
Configuration SA StandAlone
BT BenchTop
IT Integrated
StandAlone units are intended for operation on the ground while BenchTop
models are for use on a table. The transfer position in SA units is 1000mm high
while on BT units it is ~200mm.
Capacity STX40 44 MTP capacity
STX100 110 MTP capacity
STX200 198 MTP capacity
STX500 250 DWL capacity (530 MTP)
SXT1000 500 DWL capacity (1060 MTP)
StoreX SA and BT units may be stacked.
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StoreX Series STX_V078.doc 10 CMa, 23.11.2009
2.4 LiCONiC StoreX Climate Overview
Climate IC Incubator ~33..50°C >95% RH
HC Humid Cooler 04..20°C 90..95% RH
DC Dry Cooler 04..20°C <5%RH
HR Humid Range 04..70°C 90..95%RH
DR Dry Range 04..50°C <5%RH
DF Deep Freezer -20°C
The graphical chart below depicts an overview of the various climate ranges
covered by each climate option. The vertical axis shows the relative humidity
while the horizontal axis shows temperature. It can be clearly seen that the units
cover both extremes: extreme humidity and extreme dryness.
HR-units are Wide Range instruments that cover a wide temperature range. They
are a combination, with integrated heater and cooler. Note that all cooled StoreX
have an integrated active cooling system. No additional external cooler is
needed.
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StoreX Series STX_V078.doc 11 CMa, 23.11.2009
2.5 The StoreX 40 Handler
Inside the StoreX climate chamber there is a handling system that allows random
transport of plates. Plates can be moved internally as well as transferred to and
from the environment. Access times are short and the internal climate is kept
stable even for short time periods between accesses.
A Handler (4) is used for vertical transport as well as loading and unloading of
samples. The Handler consists of a Vertical Positioning Drive (40), a Turn Drive
(42) and a Shovel Drive (43). The Handler has a number of vertical positions
which are determined by the number of stacker levels and the loading or
unloading level.
A Stepper motor is used for vertical positioning of the Handler. All vertical
positions are defined by a z-Initiator (15) and the software z-Offset (DM20). The
level height is determined by the number of steps of height. The actual travel
path to a certain stacker level is calculated by multiplying the level (DM5) by the
pitch (DM21) and then adding the z-Offset (DM20). The z-positions of the
Transfer Station are stored in Data Memories (DM22, DM24).
The Handler is not only used for vertical transport of the samples, it is also used
to pick and place plates in the stackers and to get from, or put plates on the
Transfer Station. The pick / place and get / put actions are vertical movements
with the extended shovel. Beware of the difference in vertical travel for pick /
place movement (DM21), get movement (DM26) and put movement (DM28).
The Turn Drive is used to face the Shovel towards the Transfer Station or
towards the desired stacker. The Turn Drive may be positioned at three turn
positions. The position at the left stacker is stored in Data Memory DM80, the
turn position at the right in Data Memory DM81 and the turn position at the
Transfer Station is stored in Data Memory DM82.
The radial Shovel motion is between two hard stops. Force and speed of this
drive is controlled by the hardware of the controller card. Speed and force can be
adjusted by authorized service personnel.
Lift Assembly
Plate Lift
Plate Shovel
Stacker Plate
Stacker
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StoreX Series STX_V078.doc 12 CMa, 23.11.2009
The correct sequence of motions is monitored by initiators, by detecting the end-
positions of motions. Initiators are foreseen for Shovel-In (11) and Shovel-Out
Position (12) and Turn Save Range (13).
For save operation, Gate-Open and Gate-Close Positions are detected. The Gate
may only be closed when the Turn Save Initiator is active indicating the Handler
is turned in.
The Transfer Station is the location where the samples are taken by or put on by
the StoreX and the surrounding system. The Transfer Station acts as the
common loading / unloading position of the StoreX and the surrounding system.
There are several different types of Transfer Stations. This will allow simple
integration of the StoreX toany existing system.
2.6 The StoreX Carrousel Handler
Inside the StoreX climate chamber of higher capacity instruments there is a
handling system that includes a positionable carrousel thus allowing random
access and transport of plates. Plates can be moved internally as well as
transferred to and from the environment. Access times are short and the internal
climate is kept stable even for short time periods between accesses.
A Handler is used for vertical transport as well as loading and unloading of
samples. The Handler consists of a Vertical Positioning Drive, a Turn Drive and a
Shovel Drive an a Rotating Drive. The Handler has a number of vertical positions
which are determined by the number of stacker levels and the loading or
unloading level.
Lift
Assembly Carrousel
Stacker
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StoreX Series STX_V078.doc 13 CMa, 23.11.2009
3. Options
3.1.1 Overview
The LiCONiC StoreX is available with a wide selection of options. By choosing
the right options the StoreX can be optimized for almost all known applications.
The range of options is continuously growing. The list of options listed below is
not a complete list. Please contact Liconic if you need functions not listed in this
manual.
NOTE !
 In most cases it is not possible to retrofit options in the field
3.2 StoreX Climate Options
3.2.1 StoreX IC
(Incubator System)
3.2.2 StoreX HC
(Humid Cooling System)
3.2.3 StoreX DC
(Dry Cooling System)
3.2.4 StoreX HR
(Humid Wide Range Cooling and Heating System)
3.2.5 StoreX DR
(Dry Cooling and Heating System)
3.2.6 StoreX DF
(Deep Freezer System)
3.3 StoreX Configurations
3.3.1 StandAlone (SA)
StandAlone configured units are intended for ground floor operation. These units
have the Gate located in the upper part of the unit. The transfer position is at
approximately at the height of a typical table (1000mm). A StandAlone unit can
therefore be placed next to a table or a robotic instrument.
The instrument’s controls and climate generator are located below the incubation
chamber. StoreX SA (StandAlone) may easily be lifted and moved by a forklift.
The top cover may be used as a table and can hold other instruments.
The picture below shows a StoreX STX200 in StandAlone configuration. Note
that all StoreX models are available in StandAlone configuration. Larger capacity
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StoreX Series STX_V078.doc 14 CMa, 23.11.2009
models are available as StandAlones only. A StandAlone StoreX may carry a
BenchTop configured StoreX.
3.3.2 BenchTop (BT)
BenchTop (BT) configured units are intended for operation on a table or on top of
a StandAlone configured StoreX. These units have the Gate located in the lower
part of the unit. The transfer position is kept as close to the table surface (as low)
as possible (200mm). This configuration is used when the StoreX is integrated on
an existing table.
The instrument’s controls and climate generator are located above the incubation
chamber. The picture below shows a StoreX STX40 in BenchTop configuration.
Note that only low capacity (up to ~200 plates) StoreX models are available in
BenchTop. Larger capacity models are available as StandAlones only.
User Front
Door
Ventilation Slits
Display
Front Panel
Display
User Front Door
Front Panel
Ventilation Slits
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StoreX Series STX_V078.doc 15 CMa, 23.11.2009
3.3.3 Copper Climate Chamber
All StoreX are available with a full copper incubation chamber. This feature
ensures a maximum protection against cross-contamination inside the incubation
chamber.
3.4 RH-Option
The RH Option allows the display and control of humidity inside the incubation
chamber. A very high quality sensor is used in this option. Quality and accuracy
of the sensor is maintained by the Swiss Federal Office of Metrology and the
Swiss Accreditation Service.
Principle of Operation
The Humidity sensors used in the StoreX series are suitable for extremely high
humidity as well as for extreme dryness. They incorporate a humidity and a
temperature sensor allowing a relative humidity (RH) readout.
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StoreX Series STX_V078.doc 16 CMa, 23.11.2009
Specifications
Measuring Range 0..100 % RH
Accuracy@23°C ±1.5
±1.3
% RH
°K Temp.
Resolution 12
12
Bit / 0.02%
Bit / 0.1°C
Sensor PT100
C94
Temperature
Humidity
Adjustment - E2-PROM
3.5 Gassing Options
3.5.1 Gassing Conditioner
The Gassing conditioner is required for Gassing Options. Note that the Gassing
Conditioner is required one time regardless of the number of Gassing Options
used in a system.
3.5.2 CO2 Carbon Dioxide Option
As StoreX incubators have to cope with frequent accesses they use a very
advanced CO2 controller that is extremely accurate, fast and stable.
The CO2 Option of StoreX incubators consists of
• Gassing conditioner
• Gassing Controller
• Measurement Cell
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StoreX Series STX_V078.doc 17 CMa, 23.11.2009
Principle of Operation
The infra-red spectra of many gases are characterized by narrow, non-
overlapping absorption bands. Thus, the measurement of absorption within a
defined wavelength band can uniquely identify the presence and concentration of
a particular gas.
In NDIR gas sensors, an infrared optical 'sample' filter with a narrow band of
transmission is selected to overlap with the absorption band of interest, the
position of the filter determines which gas will be measured and the amount of
absorption determines the gas concentration present.
The infrared filter is chosen to ensure that the sensor is totally gas specific and
will not exhibit cross sensitivity to other gases.
Specifications
Measuring Range 0..10 % Vol.
Input Pressure 2
8
bar typ.
bar max.
Gas Flow ~10 l/min max.
Gas Consumption @ 5% CO2, 37°C S <1
~7
~18
l/h standby
l/h 2-min.-access cycles
l/h 30sec.-access cycles
Measuring Wavelength 4200 nm
Accuracy ±3 % FS
Stability ±5 % over 12 month
Repeatability ±0.5 % at zero
% at full scale
Response Time ~30 s
Warm-Up Time 5
30
min. operation
min full accuracy
MTBF 5 years
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StoreX Series STX_V078.doc 18 CMa, 23.11.2009
3.5.3 N2 Nitrogen Option
As StoreX incubators have to cope with frequent accesses they use a high
quality N2 controller. The nitrogen measurement is based on an oxygen
measurement. The N2 option therefore assumes that oxygen is displaced by
nitrogen.
The N2 Option of StoreX incubators consists of
• Gassing conditioner
• Gassing Controller
• Measurement Cell
Principle of Operation
A zirconia electrolyte disc with electrodes on both sides has the ability to pass
oxygen ions at high temperature.
By attaching a cap with an aperture on one side (cathode) of the disc, the gas
diffusion is limited and the saturated current is observed. It is termed "limiting
current". The limiting current is proportional to the ambient oxygen concentration.
Specifications
Measuring Range 75..99.9 % Vol.
Input Pressure 2
8
bar typ.
bar max.
Gas Flow ~10 l/min max.
Gas Consumption @ 95% N2, 20°C <__
~__
~__
l/h standby
l/h 2-min.-access cycles
l/h 30sec.-access cycles
Accuracy ±0.5 % FS
Stability ±0.5 % FS over 12 month
Repeatability ±1 % at full scale
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StoreX Series STX_V078.doc 19 CMa, 23.11.2009
Response Time ~30 s
Warm-Up Time 5 min. operation
MTBF 30’000 hrs
3.5.4 O2 Oxygen Option
The sensors for oxygen measurement are based on the same principles as
described above. The main difference is the measurement range of the sensor
used in the O2 option.
Specifications
Measuring Range 0.1..95 % Vol.
Input Pressure 2
8
bar typ.
bar max.
Gas Flow ~10 l/min max.
Gas Consumption @ 25% O2, 20°C <__
~__
~__
l/h standby
l/h 2-min.-access cycles
l/h 30sec.-access cycles
Accuracy ±0.5 % FS
Stability ±0.5 % FS over 12 month
Repeatability ±1 % at full scale
Response Time ~30 s
Warm-Up Time 5 min. operation
MTBF 30’000 hrs
WARNING !
O2-Option leads to increased explosion risk
The maximum concentration of oxygen allowed with this option is limited to 25%
O2 vol. for safety reasons.
3.5.5 Other Gassing Options
There are other gassing options available. Please contact Liconic applications for
further information.
3.6 UHS (Ultra High Speed) Option
Applications with very short access times. The access speed of the UHS handler
is almost double compared to standard High Speed handlers. Access time for
UHS STX40 units is maximum ~12 seconds and for larger units access time is
maximum ~20 seconds. Please ask for the latest specifications for your model.
3.7 Customized Color
Standard coating of the StoreX is RAL 9016. Other customized coating is
possible. Please specify RAL code and/or send sample. Note that this option will
increase delivery time.
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