Lightnovo miniRaman User manual

miniRaman microscope
version 1.0
English
lightnovo.com
User Manual

2
Table of Contents
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
General Information . . . . . . . . . . . . . . . . . . . . . 4
Warning labels . . . . . . . . . . . . . . . . . . . . . . . . 5
Safety instructions . . . . . . . . . . . . . . . . . . . . . . 5
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation . . . . . . . . . . . . . . . . . . . . . . . . 8
General Information . . . . . . . . . . . . . . . . . . . . . 8
Delivery Scope . . . . . . . . . . . . . . . . . . . . . . . . 8
Site requirements . . . . . . . . . . . . . . . . . . . . . . 9
Cable Connections . . . . . . . . . . . . . . . . . . . . . . 10
Software Installation . . . . . . . . . . . . . . . . . . . . . 11
Removal and installation of miniRaman spectrometer . . . . . . . . . 12
Overview . . . . . . . . . . . . . . . . . . . . . . . . . 13
General information. . . . . . . . . . . . . . . . . . . . . . 13
Specication . . . . . . . . . . . . . . . . . . . . . . . . 14
Instrument design . . . . . . . . . . . . . . . . . . . . . . 16
General overview of control elements and components . . . . . . . . 17
Operation . . . . . . . . . . . . . . . . . . . . . . . . . 18
Hardware connection . . . . . . . . . . . . . . . . . . . . . 18
Operation procedure . . . . . . . . . . . . . . . . . . . . . 19
Upright and Inverted operation . . . . . . . . . . . . . . . . . 28
Technical drawings. . . . . . . . . . . . . . . . . . . . 29
Service addresses . . . . . . . . . . . . . . . . . . . . 30

4
Safety
1General Information
Read the following safety instructions carefully before operating miniRaman micro-
scope and keep this manual for future reference available at any time. Always observe
the instructions described in this manual to ensure user safety and to avoid property
damage. Improper use or failure to follow these safety instructions can result in seri-
ous injuries and/or property damage. Any non-observance of the precautions will
infringe the intended use (i.e. performing measurements by Raman spectroscopy) of
miniRaman microscope. In this case Lightnovo ApS will not assume any liability. It is the
operator’s duty to plan and implement all necessary safety measures and to supervise
their observance. Moreover, the operator must ensure that miniRaman microscope is
in proper functioning condition. A safe and faultless operation can only be guaranteed
if miniRaman microscope is transported, stored, installed, operated and maintained
properly according to the procedures described in this manual. Never remove or deac-
tivate any supporting safety systems during miniRaman microscope operation. Ensure
that objects and/or material not required for the measurement is out of the instrument
operating area.
Qualied Personnel
Primary installation and all maintenance and repair works not described in this manual
should only be performed by Lightnovo service personnel. Only authorized operat-
ing personnel that have been briefed about the instrument operation and all relevant
safety aspects should operate and maintain (i.e. only maintenance works that are de-
scribed in this manual) the instrument. All repairs, adjustments and alignments on any
miniRaman microscope component must be performed in accordance with the safety
regulations and standards applied in the country in which the instrument is installed.
Correct Usage
miniRaman microscope and its components should only be used according to the
instructions described in the manual or advised by a Lightnovo engineer. In case of
accessories or components made by other manufacturers and used in connection with
the microscope, Lightnovo does not assume any liability for safe operation and proper
functioning.

5
Safety
2
3
Warning labels
Safety instructions
This warning symbol indicates the existence of laser radia-
tion. A Class 3R laser is considered safe if handled carefully,
with restricted beam viewing. With a class 3R laser, the maxi-
mum permissible exposure (MPE) can be exceeded, but with
a low risk of injury. Visible continuous lasers in Class 3R are limited to 5 milliwatts.
This warning symbol indicates the existence of laser radia-
tion. Class 3B lasers are hazardous for eye exposure. They
can heat skin and materials but are not considered a burn
hazard. For visible-light lasers, Class 3B lasers’ output power
is between 5 and 499 milliwatts. Class 3B lasers are normally
hazardous under direct beam viewing conditions, but are normally safe when viewing
diuse reections.
The following chapters describe all relevant safety aspects of the instrument operation.
Depending on the degree of hazard the safety instructions are classied as follows:
Danger
indicates that death, severe personal injury or substantial property damage WILL result
if proper precautions are not taken.
Warning
indicates that death, severe personal injury or substantial property damage CAN result
if proper precautions are not taken.
Caution
indicates that minor personal injury or property damage CAN result if proper precau-
tions are not taken. Important draws your attention to a particularly important infor-
mation.
Note
draws your attention to an useful information on the product, e.g. product operation
or to a special part of the manual.
The safety instructions Danger, Warning and Caution are marked by the corresponding
warning labels.

6
4Laser Safety
General Information
The analysis system miniRaman microscope uses the light of a lasers (660 and 785nm)
that are installed in miniRaman spectrometer. The used laser diodes emit visible and
partially visible laser radiation in the near infrared region.
According to the standard EN 60825-1/10.2003, model MiniRaman microscope SERS
is laser class 3R product and it is considered safe if handled carefully, with restricted
beam viewing.
According to the standard EN 60825-1/10.2003, miniRaman microscope models:
MiniRaman microscope Standard, MiniRaman microscope Standard Dual, MiniRaman
microscope Power Dual, MiniRaman microscope Power are Class 3B lasers products.
Therefore, they are normally hazardous under direct beam viewing conditions, but are
normally safe when viewing diuse reections.
Safety Features
The laser protective enclosure shields the area around the sample stage. The enclosure
is opened and closed manually. For safety reasons, the operator has to close the laser
protective enclosure before starting measurement. This safety precaution protects the
operator against accidental exposure to hazardous laser radiation exiting the objective.
Figure 1. Open Laser Protective Enclosure Closed Laser Protective Enclosure
Safety

7
Safety Instructions
In additions to the safety instructions given below, also comply with all local regulations
concerning laser safety.
The analysis system is specied as a laser class 3R/3B product (depending on the
model, see overview section), i.e. it considered safe if handled carefully, with restricted
beam viewing. Nonetheless, observe the following safety instructions:
Warning:
Avoid eye and skin exposure to direct or scattered laser radiation under
all circumstances! Failure to do so can cause permanent and irreversible
eye damage and/or serious skin injuries!
Do NOT remove the laser protective enclosure!
Safety

8
Installation
1
2
General Information
Delivery Scope
Unpacking and initial installation including hardware setup and cable connection is
done by qualied Lightnovo service personnel. The operating company has to provide
the required utilities and an installation site that meets the site requirements described
in this chapter.
Standard Components
miniRaman microscope (including user manual and quality test report)
Several cables: power cord, USB cable. (See section Cable Connections below in this
chapter.)
Accessories (includes spares, adaptors, objective lenses, sample preparation tools etc.)
Inspecting the Packaging
After having received miniRaman microscope, inspect the packaging for damages. If
there are any signs of damage, contact your local shipping representative before open-
ing the shipping box.
Warning:
Do not put miniRaman microscope into operation if there are signs of damage. Failure
to do so may result in severe personal injuries and/or property damage.
Transportation
When transporting
the microscope, use
the original case to
avoid damages.
Figure 2. Transportation
case for miniRaman micro-
scope

9
Installation
3Site requirements
Space Requirements
miniRaman microscope requires a space of 17cm in diameter and 28cm in height. (For
the exact instrument dimensions refer to Specication.) At the rear instrument side,
take a clearance of at least 5cm into account.
When dening the installation site, take into consideration that the power supply con-
nections are easily accessible at any time.
Environmental Requirements
To ensure optimum instrument performance and long-term reliability the following
environmental conditions are essential:
• Ambient temperature range:18 - 35°C (64 - 95°F)
• Humidity (non-condensing): ≤ 70% (relative humidity)
Temperature variations can impair the results of long-term measurements. Therefore,
the temperature variations should be less than 1°C an hour and not more than 2°C per
day for high spatial resolution type of measurements.
Vibration
Ideally, the instrument is not installed near vibration sources (e.g. ventilation hoods, air
conditioners, motors, elevator).
Power Supply
The instrument power supply unit automatically adapts to the most common power
sources.
Valid voltage range:
• 100V microscope system: 100V to 120V AC 10%
• 200V microscope system: 220V to 240V AC 10%
• Valid frequency range: 50/60Hz
MiniRaman microscope is an instrument of protection class I (electric safety).
Note:
A power outlet box to which miniRaman microscope connected provides a suitable solution.
miniRaman microscope requires a stable and horizontal base which can carry the instru-
ment (ca. 7kg) and possible accessories. This is critical for applications that require high
spatial resolution.
Note:
For some kinds of application, the opportunity to black out the room is advantageous

10
Installation
4Cable Connections
Caution:
To avoid personal injury and spectrometer damage, connect miniRa-
man microscope only to a socket outlet with earthing contact.
Please unpack miniRamaman
microscope and remove it from the
transportation case (see Figure 3).
Please remove power adaptor
(Figure4) and connect it to the right
connector on the rear panel of the
miniRaman microscope (Figure 5).
Then connect USB cable to the left
connector on the rear panel and
connect it to PC from the other side.
Figure 3. miniRaman microscope with transportation case.
Figure 4. Power adaptor.